Objective:To screen the best prescription and technology of Callicarpa nudiflora film,and to study and establish the quality standard of the flim.Methods:The orthogonal test was used to investigate the effect of PVA c...Objective:To screen the best prescription and technology of Callicarpa nudiflora film,and to study and establish the quality standard of the flim.Methods:The orthogonal test was used to investigate the effect of PVA content,glycerin content,ethanol and water ratio on the comprehensive score of the coating agent;the general inspection method and content determination method were used to control the quality of Callicarpa nudiflora film,and the quality standard of the film was established.Results:The best formulation was selected as polyvinyl alcohol 6.0g,glycerin 2ml,ethanol:water 1:1.The appearance,stability,heat and cold resistance of the film were good,and the quality standard of the film was established.Conclusion:The formulation is simple and feasible,and the quality control method of the film is stable and feasible.展开更多
Objective:To evaluate the antidiabetic effect of Callicarpa nudiflora extract in streptozotoc-ininduced diabetic rats.Methods:The chemical constituents in Callicarpa nudiflora extract were identified by UPLCQ-TOF-MS.C...Objective:To evaluate the antidiabetic effect of Callicarpa nudiflora extract in streptozotoc-ininduced diabetic rats.Methods:The chemical constituents in Callicarpa nudiflora extract were identified by UPLCQ-TOF-MS.Callicarpa nudiflora extract(0.15 and 0.3 g/kg)was orally administered to streptozotocin-induced diabetic rats for 42 d.The effects of Callicarpa nudiflora extract on body weight,blood glucose,serum insulin,total cholesterol,triglyceride,LDL-C and HDL-C were investigated,and its effect on liver and pancreatic pathology was assessed by histopathological analysis.Moreover,the expression levels of adenosine 5’-monophosphate-activated protein kinase(AMPK),glucose transporter type 4(GLUT4),phospho-AMPK/AMPK,and p-acetylcoA carboxylase(P-ACC)/ACC in the skeletal muscles and liver were determined by reverse transcription-polymerase chain reaction,Western blotting,and immunohistochemistry.Results:A total of 34 compounds,including 8 iridoids,14 phenylpropanoids,and 12 flavonoids,were identified from Callicarpa nudiflora extract.Callicarpa nudiflora extract significantly reduced blood glucose and significantly restored all other biochemical parameters to near normal levels,including serum insulin,total cholesterol,triglyceride,LDL-C,and HDL-C.Callicarpa nudiflora extract improved insulin resistance and reversed the damage in the liver and pancreas caused by diabetes.Furthermore,Callicarpa nudiflora extract increased the expression levels of phospho-AMPK,GLUT4,P-ACC,and insulin receptor substrate-1 and decreased the expression level of PPAR r in diabetic rats.Conclusions:Callicarpa nudiflora extract improved oral glucose tolerance,lipid metabolism,insulin resistance,and reversed the diabetes-related damage in the liver and pancreas by activating the AMPK-ACC pathway.展开更多
A phytochemical investigation was carried out on the extract of a medicinal plant Callicarpa nudiflora,resulting in the characterization of five new 3,4-seco-isopimarane(1-5)and one new 3,4-seco-pimarane diterpenoid(6...A phytochemical investigation was carried out on the extract of a medicinal plant Callicarpa nudiflora,resulting in the characterization of five new 3,4-seco-isopimarane(1-5)and one new 3,4-seco-pimarane diterpenoid(6),together with four known compounds.The structures of the new compounds were fully elucidated by extensive analysis of MS,1 D and 2 D NMR spectroscopic data,and time-dependent density functional theory(TDDFT)calculation of electronic circular dichroism(ECD)spectra,and DFT calculations for NMR chemical shifts and optical rotations.展开更多
Four new 3,4-seco-labdane diterpenoids,nudiflopenes J-M,were isolated from the leaves of Callicarpa nudiflora along with six known compounds.The structures of these diterpenoids were determined by comprehensive spectr...Four new 3,4-seco-labdane diterpenoids,nudiflopenes J-M,were isolated from the leaves of Callicarpa nudiflora along with six known compounds.The structures of these diterpenoids were determined by comprehensive spectroscopic analysis.All the isolated compounds were evaluated for their inhibitory effects on NO production in LPS-stimulated RPMs and RAW264.7 cells.The results suggest that nudiflopenes J-M and other four known compounds showed significant inhibitory effects against NO production comparable to the positive control dexamethasone.展开更多
基金Hainan Natural Science Foundation 2018(No.2018CXTD339)
文摘Objective:To screen the best prescription and technology of Callicarpa nudiflora film,and to study and establish the quality standard of the flim.Methods:The orthogonal test was used to investigate the effect of PVA content,glycerin content,ethanol and water ratio on the comprehensive score of the coating agent;the general inspection method and content determination method were used to control the quality of Callicarpa nudiflora film,and the quality standard of the film was established.Results:The best formulation was selected as polyvinyl alcohol 6.0g,glycerin 2ml,ethanol:water 1:1.The appearance,stability,heat and cold resistance of the film were good,and the quality standard of the film was established.Conclusion:The formulation is simple and feasible,and the quality control method of the film is stable and feasible.
基金supported by the Key Research&Development Plan of Hainan Province(No.ZDYF2018228)Natural Science Foundation of Guangdong Province(No.2016A030313034).
文摘Objective:To evaluate the antidiabetic effect of Callicarpa nudiflora extract in streptozotoc-ininduced diabetic rats.Methods:The chemical constituents in Callicarpa nudiflora extract were identified by UPLCQ-TOF-MS.Callicarpa nudiflora extract(0.15 and 0.3 g/kg)was orally administered to streptozotocin-induced diabetic rats for 42 d.The effects of Callicarpa nudiflora extract on body weight,blood glucose,serum insulin,total cholesterol,triglyceride,LDL-C and HDL-C were investigated,and its effect on liver and pancreatic pathology was assessed by histopathological analysis.Moreover,the expression levels of adenosine 5’-monophosphate-activated protein kinase(AMPK),glucose transporter type 4(GLUT4),phospho-AMPK/AMPK,and p-acetylcoA carboxylase(P-ACC)/ACC in the skeletal muscles and liver were determined by reverse transcription-polymerase chain reaction,Western blotting,and immunohistochemistry.Results:A total of 34 compounds,including 8 iridoids,14 phenylpropanoids,and 12 flavonoids,were identified from Callicarpa nudiflora extract.Callicarpa nudiflora extract significantly reduced blood glucose and significantly restored all other biochemical parameters to near normal levels,including serum insulin,total cholesterol,triglyceride,LDL-C,and HDL-C.Callicarpa nudiflora extract improved insulin resistance and reversed the damage in the liver and pancreas caused by diabetes.Furthermore,Callicarpa nudiflora extract increased the expression levels of phospho-AMPK,GLUT4,P-ACC,and insulin receptor substrate-1 and decreased the expression level of PPAR r in diabetic rats.Conclusions:Callicarpa nudiflora extract improved oral glucose tolerance,lipid metabolism,insulin resistance,and reversed the diabetes-related damage in the liver and pancreas by activating the AMPK-ACC pathway.
基金supported from the China National Key R&D Program(Nos.2017YFE0195100 and 2018YFC.707000)the National Natural Science Foundation of China(Nos.21920102003 and 81903485)。
文摘A phytochemical investigation was carried out on the extract of a medicinal plant Callicarpa nudiflora,resulting in the characterization of five new 3,4-seco-isopimarane(1-5)and one new 3,4-seco-pimarane diterpenoid(6),together with four known compounds.The structures of the new compounds were fully elucidated by extensive analysis of MS,1 D and 2 D NMR spectroscopic data,and time-dependent density functional theory(TDDFT)calculation of electronic circular dichroism(ECD)spectra,and DFT calculations for NMR chemical shifts and optical rotations.
基金supported by the National Natural Science Foundation of China(No.81503224)Guangdong Key Laboratory for translational Cancer research of Chinese Medicine(No.2018B030322011)+1 种基金Department of education of Guangdong Province(No.2016KZD-XM031)Science and Technology Project of Guangzhou City(No.201707010467)
文摘Four new 3,4-seco-labdane diterpenoids,nudiflopenes J-M,were isolated from the leaves of Callicarpa nudiflora along with six known compounds.The structures of these diterpenoids were determined by comprehensive spectroscopic analysis.All the isolated compounds were evaluated for their inhibitory effects on NO production in LPS-stimulated RPMs and RAW264.7 cells.The results suggest that nudiflopenes J-M and other four known compounds showed significant inhibitory effects against NO production comparable to the positive control dexamethasone.
