The mechanism of Callistephus chinensis flavonoid compounds in the treatment of diabetes using network pharmacology and molecular docking

The purpose of this project is used for exploring the mechanism of Callistephus chinensis in the treatment of diabetes by network pharmacology and molecular docking methods.The target of Callistephus chinensis was obtained from SwissTargetPrediction database,while the target related to diabetes was obtained from GeneCards and OMIM databases.The target was added in String database to build the protein interaction network.GO biological process enrichment analysis and KEGG pathway enrichment analysis were carried out by Metascape software,then the target-pathway network was constructed.Molecular docking was carried out in Discovery Studio 2016 Client software to verify the binding force of Callistephus chinensis flavonoid compounds with key targets.In this study,10 potential active components were selected from the flavonoid monomer compounds of Callistephus chinensis.1847 biological processes(BP),126 cell compositions(CC)and 256 molecular functions(MF)were obtained by GO enrichment analysis;a total of 194 pathways were involved in KEGG enrichment analysis of 192 cross targets.Network analysis showed that quercetin was the main active component of flavonoids in the treatment of diabetes,AKT1,TNF,VEGFA,EGFR,SRC and other related signals were in relation to the treatment of diabetes.This study showed that Callistephus chinensis flavonoid compounds play a role in the treatment of diabetes by regulating multi-target and multi-pathway.

Effects of osmopriming and hydropriming on vigour and germination of China aster(Callistephus chinensis(L.) Nees.) seeds

The aim of this research was to evaluate the effects of osmopriming and different hydropriming treatments on the vigour and germination of China aster （Callistephus chinensis） seeds. Seed vigour and germination tests were conducted at 10, 20 and 30℃ in darkness for untreated, osmoprimed and hydroprimed seeds. The following parameters and categories of seeds and seedlings were evaluated： the mean germination time （MGT）, T10, T50, U75-25, U90-10, the percentage of germinating seeds （Gmax）, germination capacity, percentage of abnormal seedlings and dead seeds. The results showed that osmopriming accelerated seed germination to the largest extent and improved the uniformity of germination at 10 and 30℃. Among the hydropriming treatments the highest speed of germination was observed for seeds hydrated in 500 μL of water per 1 g of seeds for 48 h at 15℃. This treatment accelerated seed germination at 10℃ compared with the control. Osmopriming increased seed germination capacity at 30℃. None of the applied hydropriming treatments improved this parameter.

FLAVONOIDS AND ITS DERIVATIVES FROM CALLISTEPHUS CHINENSIS FLOWERS AND SIMULTANEOUS DERTERMINATION BY HPLC

The aim of the study was to study the chemical constituents of Callistephus chinensis flowers(CCF)(Chinese name is Cui Ju)and establish a method for determination of seven flavonoids in CCF by using HPLC simultaneously.The compounds were isolated by repeated column chromatography with silica gel,and HPLC.