Plant calmodulin(CaM) has been extracted from cauliflower, and the purified CaM has been identified with the activation of NAD kinase(NADK) and the inhibition effect of CaM antagonist W 7. CaM′s intrinsic fluorescen...Plant calmodulin(CaM) has been extracted from cauliflower, and the purified CaM has been identified with the activation of NAD kinase(NADK) and the inhibition effect of CaM antagonist W 7. CaM′s intrinsic fluorescence and Tb 3+ fluorescence showed that there was one tyrosine residue and four metal binding sites in cauliflower CaM. Based on Frster type nonradiative energy theory, the distances of Tyr→site Ⅲ, Ⅳ have been determined , and these are 1 23 nm(Tyr→site Ⅲ) and 1 18 nm(Tyr→site Ⅳ). The Eu 3+ and Tb 3+ fluorescence probes showed that the combination of CaM with W 7 resulted in significant change on CaM′s conformation, but did not affect coordination environment of metal binding sites.展开更多
文摘Plant calmodulin(CaM) has been extracted from cauliflower, and the purified CaM has been identified with the activation of NAD kinase(NADK) and the inhibition effect of CaM antagonist W 7. CaM′s intrinsic fluorescence and Tb 3+ fluorescence showed that there was one tyrosine residue and four metal binding sites in cauliflower CaM. Based on Frster type nonradiative energy theory, the distances of Tyr→site Ⅲ, Ⅳ have been determined , and these are 1 23 nm(Tyr→site Ⅲ) and 1 18 nm(Tyr→site Ⅳ). The Eu 3+ and Tb 3+ fluorescence probes showed that the combination of CaM with W 7 resulted in significant change on CaM′s conformation, but did not affect coordination environment of metal binding sites.
