北川驴蹄草(Caltha dysosmoides Tao Zhang et al)是2016年新命名的物种(四川特有),为保护利用这一新种,急需研究其生殖生物学特征。本研究于不同种群中选取50株进行标记,观察其花部特征,测试花粉活力以及柱头可授性。采用TTC法测定花...北川驴蹄草(Caltha dysosmoides Tao Zhang et al)是2016年新命名的物种(四川特有),为保护利用这一新种,急需研究其生殖生物学特征。本研究于不同种群中选取50株进行标记,观察其花部特征,测试花粉活力以及柱头可授性。采用TTC法测定花粉活力;采用液体培养基对北川驴蹄草花粉进行离体培养;采用联苯胺-过氧化氢法测定柱头可授性(直到无活力为止)。实验结果表明:在自然条件下,北川驴蹄草4月~5月开花,单花花期一般为2 d。雄蕊短于雌蕊,花药紧贴花柱外侧,花药与柱头相距约3 mm,在整个花朵开放的过程中雌蕊和雄蕊的相对位置不变;花粉活力早上明显低于下午,且于花后1 h^4 h内增长较快,4h之后花粉活力增长较慢;柱头具有可授性,于花后3 h时柱头可授性相对较强。展开更多
The methanolic extract obtained from the root portion of Caltha palustris var. alba was evaluated for its anthelmintic efficacy against gastrointestinal nematodes of sheep under both in vitro and in vivo conditions us...The methanolic extract obtained from the root portion of Caltha palustris var. alba was evaluated for its anthelmintic efficacy against gastrointestinal nematodes of sheep under both in vitro and in vivo conditions using worm motility inhibition(WMI) assay and fecal egg count reduction(FECR) assay, respectively. The extract was subjected to antimicrobial activity using agar-well diffusion method against different bacterial strains. In addition the extract was evaluated for cytotoxic and antioxidant activity against cultured THP-1(Leukemia), A-549(Lung), HCT-15(Colon), Cervix(HeLa) and PC-3(Prostrate) cell lines by SRB and DPPH radical scavenging assays. The extract used resulted in mean %WMI of 94.44%, as observed when the worms were put in lukewarm buffer for 30 min after exposure to different treatments. The mean mortality index of the sample was 0.95. The lethal concentration(LC50) was 0.11 mg·mL-1. Cell lines were exposed to concentration of 100 μg·mL-1 of extract for 48 h, which reduced the viability of these cell lines. The same plant extract also showed 55.58% DPPH radical scavenging activity.展开更多
文摘The methanolic extract obtained from the root portion of Caltha palustris var. alba was evaluated for its anthelmintic efficacy against gastrointestinal nematodes of sheep under both in vitro and in vivo conditions using worm motility inhibition(WMI) assay and fecal egg count reduction(FECR) assay, respectively. The extract was subjected to antimicrobial activity using agar-well diffusion method against different bacterial strains. In addition the extract was evaluated for cytotoxic and antioxidant activity against cultured THP-1(Leukemia), A-549(Lung), HCT-15(Colon), Cervix(HeLa) and PC-3(Prostrate) cell lines by SRB and DPPH radical scavenging assays. The extract used resulted in mean %WMI of 94.44%, as observed when the worms were put in lukewarm buffer for 30 min after exposure to different treatments. The mean mortality index of the sample was 0.95. The lethal concentration(LC50) was 0.11 mg·mL-1. Cell lines were exposed to concentration of 100 μg·mL-1 of extract for 48 h, which reduced the viability of these cell lines. The same plant extract also showed 55.58% DPPH radical scavenging activity.
基金Supported by the National Natural Science Foundation of China(31800189,31500193)Key Program of Natural Science Research of Education Department in Anhui Province(KJ2017A358)~~