[Objectives]This study was conducted to explore active components and molecular biological mechanisms of Camellia nitidissima Chi in the treatment of premature ovarian failure(POF).[Methods]The active components and c...[Objectives]This study was conducted to explore active components and molecular biological mechanisms of Camellia nitidissima Chi in the treatment of premature ovarian failure(POF).[Methods]The active components and corresponding targets of C.nitidissima Chi were retrieved through literature and the TCMSP database.POF-related disease targets were identified using the OMIM and Genecards databases.A PPI network was constructed using the STRING database and Cytoscape 3.10.2.A herb-active component-target-pathway network diagram was also constructed with Cytoscape 3.10.2.The CytoNCA plugin was used to screen out the top five core targets and core active components.GO and KEGG enrichment analyses of the intersecting targets were performed using DAVID.Finally,molecular docking was conducted using Auto Dock to verify the interaction between core targets and active components,and visualization was done using PyMol.[Results]A total of 26 active components and 461 targets of C.nitidissima Chi were identified,with 154 intersecting targets related to POF.The core components of the herb included 1,1'-bi-2-naphthol,3',4-O-dimethylcedrusin,eriodictyol,quercetin,and vanillin.The PPI network revealed that the main targets were epidermal growth factor receptor(EGFR),protein kinase B1(AKT1),proto-oncogene Src(SRC),hypoxia-inducible factor 1α(HIF1A),and estrogen receptor 1(ESR1).KEGG enrichment analysis revealed 10 pathways closely related to POF,mainly involving the PI3K-Akt signaling pathway,chemical carcinogenesis-reactive oxygen species,endocrine resistance,and the HIF-1 signaling pathway.Molecular docking results showed that the core active components had strong binding activity with the targets.[Conclusions]C.nitidissima Chi has multi-component,multi-target,and multi-pathway characteristics in the comprehensive treatment of POF,providing informational support for its clinical application.展开更多
Camellia nitidissima is a species of shrub or small tree whose leaves and leaf buds are used to produce tea. In recent years, C. nitidissima planting area has been increasing, resulting in the growing gap between the ...Camellia nitidissima is a species of shrub or small tree whose leaves and leaf buds are used to produce tea. In recent years, C. nitidissima planting area has been increasing, resulting in the growing gap between the supply and demand of C. nitidissima seedlings. At present, C. nitidissima can be propagated through seeding, cutting, grafting, plant tissue culture and air layering. Among them, cutting and tissue culture are the most commonly used methods for rapid propagation of C. nitidissima. Optimal rooting medium, type and concentration of hormones and environmental conditions are critical factors for C. nitidissima propagation by cuttings, while detoxification and optimization of medium composition are important for C. nitidissima propagation by tissue culture. This paper reviews recent pro- gress in propagation techniques for C. nitidissima, and finally puts forward some prospects and suggestions in C. nitidissima propagation.展开更多
Objective To isolate and identify the bioactive phytochemicals from the leaves of Camellia nitidissima. Methods The chemical constituents were isolated and purified by repeated silica gel, Sephadex LH-20, MCI gel colu...Objective To isolate and identify the bioactive phytochemicals from the leaves of Camellia nitidissima. Methods The chemical constituents were isolated and purified by repeated silica gel, Sephadex LH-20, MCI gel columns, recrystallization, and semi-preparative HPLC techniques. The chemicl structures of these compounds were identified on the basis of spectral data including NMR and MS. Then quorum sensing inhibition （QSI） activities of these compounds were tested using Chromobacterium violaceum CV026 as the bioindicator strain. The antitumor activities of these compounds were measured using SGC7901 as cell proliferation and cytotoxicity. Results cx-Spinasteryl-I^-D-glucopyranoside （1）, stigmasta-7,22-diene-3-O-[c^-L-arabinopyranosyl （1 -2）]-β-D-galactopyranoside （2）, kaempferol 3-O-[2-O-（trans-p-coumaroyl）-3-O-α -D-glucopyranosyl]-α-D-glucopyranoside （3）, aromadendrin （4）, catechin （5）, phlorizin 4＇-O-β-D-glucopyranoside （6）, （3R,6R,7Lg-3-hydroxy-4,7-megastigmadien- 9-one （7）, dodecanoic acid （8）, 3α-acetoxy-20-1upanol （9）, and 3β,6α- trihydroxyolean- 7-one （1 0） were successively isolated from the leaves of C. nitidissima. Unfortunately, these compounds had no QSI activity. Based on Cell Counting Kit-8 （CCK-8） assay, compound 10 showed the best anti-tumor activity or all compounds （ICs0 = 91.7 μg/mL）. Conclusion Apart from compounds 4 and 5, other eight compounds are reported in this plant for the first time. All compounds show no QSI activity, compound 10 shows potential cytotoxic activity on SGC7901 cells in vitro.展开更多
In the study,the volatile compounds in two Camellia chrysantha(Hu) Tuyama species were extracted and analysed by simultaneous steam distillation and extraction(SDE) and gas chromatography mass spectrometry(GCMS),respe...In the study,the volatile compounds in two Camellia chrysantha(Hu) Tuyama species were extracted and analysed by simultaneous steam distillation and extraction(SDE) and gas chromatography mass spectrometry(GCMS),respectively.The relative contents of the chemical constituents in the volatile components were quantified by peak area normalization and NIST database.A total of 60 volatile components were identified,45 for Camellia nitidissima and 26 for Camellia euphlebia.Aldehydes and acids were the most dominant volatiles in Camellia nitidissima,but for Camellia euphlebia there were hydrocarbons and ketones.Hexanoic acid,4-(2,6,6-trimethyl-1-cyclohexenyl)-3-buten-2-one,cis-2-decenal,4-[2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3-buten-2-one,phenylacetaldehyde,eicosane,alpha-ionone,geranylacetone 2,6-di-tert-butyl-4-methylphenol,7,9-di-tertbutyl-1-oxaspiro[4,5]deca-6,9-diene-2,8-dioi and tetradecanal were both in two Camellia chrysantha(Hu)Tuyama species.展开更多
基金Supported by Guangxi Natural Science Foundation(2020GXNSFAA238022)National Natural Sciences Foundation of China(82060280)Science and Technology Plan Project of Guangxi University of Chinese Medicine(2018ZD003).
文摘[Objectives]This study was conducted to explore active components and molecular biological mechanisms of Camellia nitidissima Chi in the treatment of premature ovarian failure(POF).[Methods]The active components and corresponding targets of C.nitidissima Chi were retrieved through literature and the TCMSP database.POF-related disease targets were identified using the OMIM and Genecards databases.A PPI network was constructed using the STRING database and Cytoscape 3.10.2.A herb-active component-target-pathway network diagram was also constructed with Cytoscape 3.10.2.The CytoNCA plugin was used to screen out the top five core targets and core active components.GO and KEGG enrichment analyses of the intersecting targets were performed using DAVID.Finally,molecular docking was conducted using Auto Dock to verify the interaction between core targets and active components,and visualization was done using PyMol.[Results]A total of 26 active components and 461 targets of C.nitidissima Chi were identified,with 154 intersecting targets related to POF.The core components of the herb included 1,1'-bi-2-naphthol,3',4-O-dimethylcedrusin,eriodictyol,quercetin,and vanillin.The PPI network revealed that the main targets were epidermal growth factor receptor(EGFR),protein kinase B1(AKT1),proto-oncogene Src(SRC),hypoxia-inducible factor 1α(HIF1A),and estrogen receptor 1(ESR1).KEGG enrichment analysis revealed 10 pathways closely related to POF,mainly involving the PI3K-Akt signaling pathway,chemical carcinogenesis-reactive oxygen species,endocrine resistance,and the HIF-1 signaling pathway.Molecular docking results showed that the core active components had strong binding activity with the targets.[Conclusions]C.nitidissima Chi has multi-component,multi-target,and multi-pathway characteristics in the comprehensive treatment of POF,providing informational support for its clinical application.
基金Supported by Guangxi Agricultural Comprehensive Development and Utilization Project from the International Fund for Agricultural Development(L-I-CN855)Science and Technology Development Project of Nanning City(20162090)+1 种基金Science and Technology Development Project of Fangchenggang City(Fang Ke AB17053004)the Fund of Guangxi Academy of Agricultural Science(Gui Nong ke 2015YZ20,Gui Nong Ke 2015YT89)
文摘Camellia nitidissima is a species of shrub or small tree whose leaves and leaf buds are used to produce tea. In recent years, C. nitidissima planting area has been increasing, resulting in the growing gap between the supply and demand of C. nitidissima seedlings. At present, C. nitidissima can be propagated through seeding, cutting, grafting, plant tissue culture and air layering. Among them, cutting and tissue culture are the most commonly used methods for rapid propagation of C. nitidissima. Optimal rooting medium, type and concentration of hormones and environmental conditions are critical factors for C. nitidissima propagation by cuttings, while detoxification and optimization of medium composition are important for C. nitidissima propagation by tissue culture. This paper reviews recent pro- gress in propagation techniques for C. nitidissima, and finally puts forward some prospects and suggestions in C. nitidissima propagation.
基金National High Technology Research and Development Program of China(863 Program)(2014AA022208)National Natural Science Foundation of China(31170131 and 31070312)Jiangsu Qinglan Project
文摘Objective To isolate and identify the bioactive phytochemicals from the leaves of Camellia nitidissima. Methods The chemical constituents were isolated and purified by repeated silica gel, Sephadex LH-20, MCI gel columns, recrystallization, and semi-preparative HPLC techniques. The chemicl structures of these compounds were identified on the basis of spectral data including NMR and MS. Then quorum sensing inhibition （QSI） activities of these compounds were tested using Chromobacterium violaceum CV026 as the bioindicator strain. The antitumor activities of these compounds were measured using SGC7901 as cell proliferation and cytotoxicity. Results cx-Spinasteryl-I^-D-glucopyranoside （1）, stigmasta-7,22-diene-3-O-[c^-L-arabinopyranosyl （1 -2）]-β-D-galactopyranoside （2）, kaempferol 3-O-[2-O-（trans-p-coumaroyl）-3-O-α -D-glucopyranosyl]-α-D-glucopyranoside （3）, aromadendrin （4）, catechin （5）, phlorizin 4＇-O-β-D-glucopyranoside （6）, （3R,6R,7Lg-3-hydroxy-4,7-megastigmadien- 9-one （7）, dodecanoic acid （8）, 3α-acetoxy-20-1upanol （9）, and 3β,6α- trihydroxyolean- 7-one （1 0） were successively isolated from the leaves of C. nitidissima. Unfortunately, these compounds had no QSI activity. Based on Cell Counting Kit-8 （CCK-8） assay, compound 10 showed the best anti-tumor activity or all compounds （ICs0 = 91.7 μg/mL）. Conclusion Apart from compounds 4 and 5, other eight compounds are reported in this plant for the first time. All compounds show no QSI activity, compound 10 shows potential cytotoxic activity on SGC7901 cells in vitro.
文摘In the study,the volatile compounds in two Camellia chrysantha(Hu) Tuyama species were extracted and analysed by simultaneous steam distillation and extraction(SDE) and gas chromatography mass spectrometry(GCMS),respectively.The relative contents of the chemical constituents in the volatile components were quantified by peak area normalization and NIST database.A total of 60 volatile components were identified,45 for Camellia nitidissima and 26 for Camellia euphlebia.Aldehydes and acids were the most dominant volatiles in Camellia nitidissima,but for Camellia euphlebia there were hydrocarbons and ketones.Hexanoic acid,4-(2,6,6-trimethyl-1-cyclohexenyl)-3-buten-2-one,cis-2-decenal,4-[2,2,6-trimethyl-7-oxabicyclo[4.1.0]hept-1-yl]-3-buten-2-one,phenylacetaldehyde,eicosane,alpha-ionone,geranylacetone 2,6-di-tert-butyl-4-methylphenol,7,9-di-tertbutyl-1-oxaspiro[4,5]deca-6,9-diene-2,8-dioi and tetradecanal were both in two Camellia chrysantha(Hu)Tuyama species.
