Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing b...Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.展开更多
The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells.The extraction of Camel...The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells.The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C_(18) column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode.The mobile phase was consisted of methanol-water(0.1% formic acid)(55:45,V/V).The total run time was 5.0 min.The method was linear in the concentration range of 0.25-250.0 ng·mL^(-1).The lower limit of quantification was 0.25 ng·mL^(-1).The intra-and inter-day relative standard deviations of entire concentration range were less than 9.3%.The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.展开更多
基金funded by the “14th Five-Year Plan” team-building projects of Guangdong Academy Agricultural Sciences (202126TD)National Natural Science Foundation of China (81903319, 81803236, 31800295)+10 种基金Guangdong Science and Technology program (2018KJYZ002)Guangdong Basic and Applied Basic Research Foundation (2020A1515011266)Qingyuan Science and Technology Program (DZXQY021, 181022114566189, 181022114566189, 2019A039, 2020KJJH042)Shaoguan Science and Technology Program (2018CS11902, 2018sn081)Guangzhou Science and Technology Program (202002030202)Zhaoqing Science and Technology Program (2019N001, 2019N013)Maoming Science and Technology Program (mmkj2020045)Zhanjiang Science and Technology Program (2020A03014)Yingde Science and Technology Board (JHXM2018029)Innovation Fund projects of Guangdong Academy of Agricultural Sciences (202115)Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science (R2019PY-JX004, R2018YJ-YB3002, R2016YJYB3003, R2018PY-QF005, R2018QD-101)。
文摘Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.
基金supported by Macao Science and Technology Development Fund(No.FDCT,006/2015/A1)Open Project of Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards(No.Guizhongzhongkai201501)
文摘The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells.The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C_(18) column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode.The mobile phase was consisted of methanol-water(0.1% formic acid)(55:45,V/V).The total run time was 5.0 min.The method was linear in the concentration range of 0.25-250.0 ng·mL^(-1).The lower limit of quantification was 0.25 ng·mL^(-1).The intra-and inter-day relative standard deviations of entire concentration range were less than 9.3%.The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.
