Background: Bringing free-living animals into captivity subjects them to the stress of both capture and captivity, leading to the alteration of normal physiological processes and behaviors through activation of the hy...Background: Bringing free-living animals into captivity subjects them to the stress of both capture and captivity, leading to the alteration of normal physiological processes and behaviors through activation of the hypothalamic– pituitary–adrenal axis. In free-living birds, although elevated plasma corticosterone (CORT) is an important adaptation regulating physiological and behavioral responses during the process of capture and captivity stress, little information is currently available on the effects of such stress on plasma metabolite levels. Methods: We examined the effects of immediate capture and 24-h captivity on body mass, body condition, plasma CORT, and metabolite levels including glucose (Glu), triglyceride (TG), total cholesterol (TC), uric acid (UA), in breeding Eurasian Tree Sparrows (Passer montanus). Results: CORT and Glu levels were increased significantly by the stress of capture, whereas TC and UA levels decreased. Body mass, body condition declined notably after 24 h in captivity, but CORT, Glu, and UA levels increased. Furthermore, male sparrows had lower TG levels after both capture and captivity than those of females. The relationships between plasma CORT and metabolite levels varied between sexes. Conclusions: Our results revealed that the metabolic status of Eurasian Tree Sparrows could be dramatically altered by capture and captivity. Monitoring the dynamic effects of both capture and captivity on plasma CORT, metabolite levels in a free-living bird contributes to a better understanding of the stress-induced pathways involved in sexdependent energy mobilization.展开更多
The breeding process, management norms and reproduction measures of African gray were briefly introduced, and the problems in breed-ing environment and reproduction process were put forward, so as to provide a referen...The breeding process, management norms and reproduction measures of African gray were briefly introduced, and the problems in breed-ing environment and reproduction process were put forward, so as to provide a reference for captive breeding of African gray.展开更多
Between June 2005 and February 2006, focal sampling and all occurrence behavior recording were used to quantify the behavioral patterns of captive female alpine musk deer (Moschus sifanicus) at Xinglongshan Musk Dee...Between June 2005 and February 2006, focal sampling and all occurrence behavior recording were used to quantify the behavioral patterns of captive female alpine musk deer (Moschus sifanicus) at Xinglongshan Musk Deer Farm, Gansu Province, China. Copulation success was used to differentiate individuals into two groups (successful and unsuccessful) and to provide a basis for behavioral comparisons, throughout both mating (rut) and non mating seasons. The results indicated significant differences between the behavior patterns of successful and unsuccessful females; however, the reproductive season played an important environmental factor. Pooling results across reproductive seasons, successfully copulating females showed significantly higher frequencies of vigilance and lower frequency of feeding behavior as compared with unsuccessfully copulating females. In the non-mating season, unsuccessfully copulating females had higher frequency of self-directed behavior, environment sniffing, and were less aggressive than successful copulating females. Furthermore, females who were successful at copulating also demonstrated tail-pasting behavior; however, this only occurred during the rut season. The results of this study can improve management practices for musk deer farms through increasing mating success and reducing maintenance costs. Furthermore, variation in behavior may also be used as a predictor of copulation success and reproductive potential, whereby females can be grouped and separated according to their reproductive history and past reproduction success.展开更多
Captivity of wild animals and restriction of their movement can lead to stress, resulting in suppressed immune response and reduced disease resistance ability. Wild animals in zoological gardens can predominately affe...Captivity of wild animals and restriction of their movement can lead to stress, resulting in suppressed immune response and reduced disease resistance ability. Wild animals in zoological gardens can predominately affected by various gastro-intestinal parasites. This study was therefore, conducted to determine occurrence, identify types and assess seasonal variation of gastrointestinal parasites in captive animals at the Zoological garden of Haramaya University. The study involved a retrospective investigation of zoo records and fecal examinations. Twenty two fecal samples were collected in December, 2013 from all the animals in the garden and examined using concentration and fecal culture techniques. Sampling was repeated after four months in April, 2014 to assess seasonal variations of the parasites. The result from the retrospective study showed death of 21 animals in the last five years. Fecal examination confirmed presence of gastrointestinal parasites with an overall mean prevalence rate of 73.8%. A total of 9 parasite taxa were identified of which 6 (66.67%) and 4 (33.33%) were helminths and protozoa respectively. No Cestode and Trematode species were recovered. <i>Trichuris spp.</i>, <i>Toxocara spp.</i>, <i>Strongyloides spp.</i>, <i>Toxascaris leonine</i>, <i>Passalurus ambiguous</i> and <i>Ascaridia spp.</i> were the helminth parasites observed in the garden. <i>Entamoeba spp.</i>, <i>Isospora spp.</i> and <i>Eimeria spp.</i> were the protozoan parasites encountered. No remarkable seasonal variation in parasitic infection was noticed in the garden. The study revealed occurrence of gastrointestinal parasites in the zoological garden, most of which are zoonotic and hence future studies are needed to determine risks of cross-transmission.展开更多
提出了滤过型固相萃取柱净化-超高效液相色谱-串联质谱法同时测定鸡蛋中全氟丁酸、全氟戊酸、全氟己酸、全氟庚酸、全氟辛酸、全氟壬酸、全氟癸酸、全氟十一酸、全氟十二酸、全氟十三酸、全氟十四酸、全氟丁烷磺酸、全氟己烷磺酸、全氟...提出了滤过型固相萃取柱净化-超高效液相色谱-串联质谱法同时测定鸡蛋中全氟丁酸、全氟戊酸、全氟己酸、全氟庚酸、全氟辛酸、全氟壬酸、全氟癸酸、全氟十一酸、全氟十二酸、全氟十三酸、全氟十四酸、全氟丁烷磺酸、全氟己烷磺酸、全氟庚烷磺酸、全氟辛烷磺酸等15种全氟化合物含量的方法。鸡蛋样品(2 g)中加入0.1 mL 20.0μg·L^(-1)同位素内标混合溶液,经10 mL 80%(体积分数)乙腈溶液振荡和超声提取后,离心;分取5 mL滤液,直接过滤过型Captive EMR-Lipid柱净化,收集流出液,氮吹至近干,加入500μL甲醇复溶,经涡旋、离心处理后测定。采用Agilent Eclipse Plus C_(18)RRHD色谱柱(100 mm×2.1 mm,1.8μm)分离,以不同体积比的2 mmol·L^(-1)乙酸铵溶液和甲醇的混合溶液梯度洗脱,在电喷雾离子源负离子扫描模式下,以多反应监测模式检测,同位素内标法定量。结果表明:15种全氟化合物标准曲线的线性范围均为0.125~20.0μg·L^(-1),测定下限(10S/N)为0.05~0.16μg·kg^(-1);在0.500,4.00,16.0μg·kg^(-1)加标浓度水平下,15种目标物的回收率为78.0%~111%,测定值的相对标准偏差(n=6)为0.87%~14%。展开更多
A healthy population of captive Amur tigers might assist recovery of the wild population in Northeast China if individuals were properly prepared and considered suitable for release in the wild.We analyzed the breedin...A healthy population of captive Amur tigers might assist recovery of the wild population in Northeast China if individuals were properly prepared and considered suitable for release in the wild.We analyzed the breeding records of 68 female Amur tigers from 1995 to 2010 in the Hengdaohezi Felid Breeding Center of China and compared the reproductive parameters of this population to wild female Amur tigers.We found that the reproductive parameters of the captive population(the age of first parturition,length of gestation and litter survival rate)were not significantly different from those of wild Amur tigers.Differences in birth date and litter size between wild and captive populations may be caused by management protocols for the captive population or insufficient field data from the wild population.Reproductive parameters of females giving birth after losing a litter were similar to parameters of females that did not lose a litter,except for birth date.These results provide no indication of major problems in using captive females for a breeding program for release of cubs into the wild,but additional information is still needed to assess their suitability.展开更多
Governmental, educational, environmental and other nonprofit organizations have placed efforts on conservation action for the threatened Mojave desert tortoise (Gopherus agassizii). While federal and state institution...Governmental, educational, environmental and other nonprofit organizations have placed efforts on conservation action for the threatened Mojave desert tortoise (Gopherus agassizii). While federal and state institutions have focused on the desert tortoise in its native habitat of the southwestern United States, there are other conservation groups whose work has been dedicated to the rescue, rehabilitation, rescue, placement and adoption of the desert tortoise in civilian homes and neighborhoods as well as sanctuaries, reserves and exhibits. AWAKE Community (AWAKE), a nonprofit organization dedicated to natural wildlife habitats and endangered species, has focused its research on desert tortoises in captivity. The California Turtle and Tortoise Club (CTTC), the official adoption arm of California’s Department of Fish and Wildlife (CDFW), has provided instruction on methods of care for desert tortoises raised in captivity including housing, feeding, sexing, health and hibernation for both adults and hatchlings. AWAKE’s research has been aimed at benefiting and supplementing the guidelines provided by CTTC, specifically for coastal climates. AWAKE’s research has also aimed to provide insight to be considered for expanding adoption possibilities to other locations, specifically tropical climates, as adoption needs for desert tortoises have increased. This manuscript presents an analysis of one coastal and one tropical climate that can serve as a basis for: 1) understanding and evaluating climate in both existing and potential habitats for desert tortoise adoptions, rescues and exhibits, 2) providing insight and special instruction for desert tortoise adoptions in coastal communities, and 3) expanding awareness around desert tortoise needs in captivity.展开更多
基金supported by the National Natural Science Foundation of China(NSFC,31672292)the Natural Science Foundation of Hebei Province(C2017205059)+1 种基金the foundation of China Scholarship Council(201408130068) to D.Lithe NSFC(31770445)to Y.Wu,the NSFC(31372201)to X.Gao
文摘Background: Bringing free-living animals into captivity subjects them to the stress of both capture and captivity, leading to the alteration of normal physiological processes and behaviors through activation of the hypothalamic– pituitary–adrenal axis. In free-living birds, although elevated plasma corticosterone (CORT) is an important adaptation regulating physiological and behavioral responses during the process of capture and captivity stress, little information is currently available on the effects of such stress on plasma metabolite levels. Methods: We examined the effects of immediate capture and 24-h captivity on body mass, body condition, plasma CORT, and metabolite levels including glucose (Glu), triglyceride (TG), total cholesterol (TC), uric acid (UA), in breeding Eurasian Tree Sparrows (Passer montanus). Results: CORT and Glu levels were increased significantly by the stress of capture, whereas TC and UA levels decreased. Body mass, body condition declined notably after 24 h in captivity, but CORT, Glu, and UA levels increased. Furthermore, male sparrows had lower TG levels after both capture and captivity than those of females. The relationships between plasma CORT and metabolite levels varied between sexes. Conclusions: Our results revealed that the metabolic status of Eurasian Tree Sparrows could be dramatically altered by capture and captivity. Monitoring the dynamic effects of both capture and captivity on plasma CORT, metabolite levels in a free-living bird contributes to a better understanding of the stress-induced pathways involved in sexdependent energy mobilization.
文摘The breeding process, management norms and reproduction measures of African gray were briefly introduced, and the problems in breed-ing environment and reproduction process were put forward, so as to provide a reference for captive breeding of African gray.
基金the National Natural Science Foundation of China (30500060, 30640023,30770286)China Post-doctoral Foundation(2005038431)+1 种基金Shanghai Post-doctoral Scientific Pro-gram of China (2005038294)the 985 Research Projects (CUN 985-03-03) of the Central University for Nationalities of China
文摘Between June 2005 and February 2006, focal sampling and all occurrence behavior recording were used to quantify the behavioral patterns of captive female alpine musk deer (Moschus sifanicus) at Xinglongshan Musk Deer Farm, Gansu Province, China. Copulation success was used to differentiate individuals into two groups (successful and unsuccessful) and to provide a basis for behavioral comparisons, throughout both mating (rut) and non mating seasons. The results indicated significant differences between the behavior patterns of successful and unsuccessful females; however, the reproductive season played an important environmental factor. Pooling results across reproductive seasons, successfully copulating females showed significantly higher frequencies of vigilance and lower frequency of feeding behavior as compared with unsuccessfully copulating females. In the non-mating season, unsuccessfully copulating females had higher frequency of self-directed behavior, environment sniffing, and were less aggressive than successful copulating females. Furthermore, females who were successful at copulating also demonstrated tail-pasting behavior; however, this only occurred during the rut season. The results of this study can improve management practices for musk deer farms through increasing mating success and reducing maintenance costs. Furthermore, variation in behavior may also be used as a predictor of copulation success and reproductive potential, whereby females can be grouped and separated according to their reproductive history and past reproduction success.
文摘Captivity of wild animals and restriction of their movement can lead to stress, resulting in suppressed immune response and reduced disease resistance ability. Wild animals in zoological gardens can predominately affected by various gastro-intestinal parasites. This study was therefore, conducted to determine occurrence, identify types and assess seasonal variation of gastrointestinal parasites in captive animals at the Zoological garden of Haramaya University. The study involved a retrospective investigation of zoo records and fecal examinations. Twenty two fecal samples were collected in December, 2013 from all the animals in the garden and examined using concentration and fecal culture techniques. Sampling was repeated after four months in April, 2014 to assess seasonal variations of the parasites. The result from the retrospective study showed death of 21 animals in the last five years. Fecal examination confirmed presence of gastrointestinal parasites with an overall mean prevalence rate of 73.8%. A total of 9 parasite taxa were identified of which 6 (66.67%) and 4 (33.33%) were helminths and protozoa respectively. No Cestode and Trematode species were recovered. <i>Trichuris spp.</i>, <i>Toxocara spp.</i>, <i>Strongyloides spp.</i>, <i>Toxascaris leonine</i>, <i>Passalurus ambiguous</i> and <i>Ascaridia spp.</i> were the helminth parasites observed in the garden. <i>Entamoeba spp.</i>, <i>Isospora spp.</i> and <i>Eimeria spp.</i> were the protozoan parasites encountered. No remarkable seasonal variation in parasitic infection was noticed in the garden. The study revealed occurrence of gastrointestinal parasites in the zoological garden, most of which are zoonotic and hence future studies are needed to determine risks of cross-transmission.
文摘提出了滤过型固相萃取柱净化-超高效液相色谱-串联质谱法同时测定鸡蛋中全氟丁酸、全氟戊酸、全氟己酸、全氟庚酸、全氟辛酸、全氟壬酸、全氟癸酸、全氟十一酸、全氟十二酸、全氟十三酸、全氟十四酸、全氟丁烷磺酸、全氟己烷磺酸、全氟庚烷磺酸、全氟辛烷磺酸等15种全氟化合物含量的方法。鸡蛋样品(2 g)中加入0.1 mL 20.0μg·L^(-1)同位素内标混合溶液,经10 mL 80%(体积分数)乙腈溶液振荡和超声提取后,离心;分取5 mL滤液,直接过滤过型Captive EMR-Lipid柱净化,收集流出液,氮吹至近干,加入500μL甲醇复溶,经涡旋、离心处理后测定。采用Agilent Eclipse Plus C_(18)RRHD色谱柱(100 mm×2.1 mm,1.8μm)分离,以不同体积比的2 mmol·L^(-1)乙酸铵溶液和甲醇的混合溶液梯度洗脱,在电喷雾离子源负离子扫描模式下,以多反应监测模式检测,同位素内标法定量。结果表明:15种全氟化合物标准曲线的线性范围均为0.125~20.0μg·L^(-1),测定下限(10S/N)为0.05~0.16μg·kg^(-1);在0.500,4.00,16.0μg·kg^(-1)加标浓度水平下,15种目标物的回收率为78.0%~111%,测定值的相对标准偏差(n=6)为0.87%~14%。
基金We are grateful for support provided through the Fundamental Research Funds for the Central Universities of China(2572014EA06 and 2572014AA14),the National Natural Science Foundation of China(NSFC31272336,31572285)and the"Study on Resource Survey Technology for Tiger and Amur Leopard Population"and"Standard of Tiger and Amur Leopard Population and Habitat Survey and Data Summarization"(State Forestry Administration).
文摘A healthy population of captive Amur tigers might assist recovery of the wild population in Northeast China if individuals were properly prepared and considered suitable for release in the wild.We analyzed the breeding records of 68 female Amur tigers from 1995 to 2010 in the Hengdaohezi Felid Breeding Center of China and compared the reproductive parameters of this population to wild female Amur tigers.We found that the reproductive parameters of the captive population(the age of first parturition,length of gestation and litter survival rate)were not significantly different from those of wild Amur tigers.Differences in birth date and litter size between wild and captive populations may be caused by management protocols for the captive population or insufficient field data from the wild population.Reproductive parameters of females giving birth after losing a litter were similar to parameters of females that did not lose a litter,except for birth date.These results provide no indication of major problems in using captive females for a breeding program for release of cubs into the wild,but additional information is still needed to assess their suitability.
文摘Governmental, educational, environmental and other nonprofit organizations have placed efforts on conservation action for the threatened Mojave desert tortoise (Gopherus agassizii). While federal and state institutions have focused on the desert tortoise in its native habitat of the southwestern United States, there are other conservation groups whose work has been dedicated to the rescue, rehabilitation, rescue, placement and adoption of the desert tortoise in civilian homes and neighborhoods as well as sanctuaries, reserves and exhibits. AWAKE Community (AWAKE), a nonprofit organization dedicated to natural wildlife habitats and endangered species, has focused its research on desert tortoises in captivity. The California Turtle and Tortoise Club (CTTC), the official adoption arm of California’s Department of Fish and Wildlife (CDFW), has provided instruction on methods of care for desert tortoises raised in captivity including housing, feeding, sexing, health and hibernation for both adults and hatchlings. AWAKE’s research has been aimed at benefiting and supplementing the guidelines provided by CTTC, specifically for coastal climates. AWAKE’s research has also aimed to provide insight to be considered for expanding adoption possibilities to other locations, specifically tropical climates, as adoption needs for desert tortoises have increased. This manuscript presents an analysis of one coastal and one tropical climate that can serve as a basis for: 1) understanding and evaluating climate in both existing and potential habitats for desert tortoise adoptions, rescues and exhibits, 2) providing insight and special instruction for desert tortoise adoptions in coastal communities, and 3) expanding awareness around desert tortoise needs in captivity.