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Glacial lakes under climate change: the imperative for comparative studies of microbial carbon metabolism in the Qinghai-Xizang Plateau and the Arctic
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作者 LIU Keshao LIU Yongqin 《Advances in Polar Science》 CSCD 2024年第3期324-326,共3页
Glacial lakes,intimately linked to glacier termini,are crucial landscape features of the Qinghai-Xizang Plateau(QXP,Qinghai-Tibet Plateau)and the Arctic.Climate warming has accelerated glacier retreat and the rapid ex... Glacial lakes,intimately linked to glacier termini,are crucial landscape features of the Qinghai-Xizang Plateau(QXP,Qinghai-Tibet Plateau)and the Arctic.Climate warming has accelerated glacier retreat and the rapid expansion of glacial lakes in both regions.Despite being typically considered harsh environments,these lakes serve as vital reservoirs for microbial biodiversity and carbon metabolism.In the face of climate change,glacial lake ecosystems over the QXP and the Arctic are undergoing unprecedentedtransformations.Thisopinioneditorial highlights the significance of conducting research and establishing long-term monitoring programs focused on microbial carbon metabolism in these glacial lakes. 展开更多
关键词 glacial lakes Qinghai-Xizang Plateau ARCTIC microbial carbon metabolism climate change glacier retreat
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Influences of Nitrogen Level on Carbon Metabolism of Spring Maize 被引量:5
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作者 其其格 李可 +2 位作者 李刚 李春艳 曹国军 《Agricultural Science & Technology》 CAS 2010年第4期34-36,共3页
[Objective]To study the effect of supplies of nitrogen level on spring maize leaf blade carbon metabolism.[Method]In this experiment,field trail and biochemistry analysis were used to study the effect of the diference... [Objective]To study the effect of supplies of nitrogen level on spring maize leaf blade carbon metabolism.[Method]In this experiment,field trail and biochemistry analysis were used to study the effect of the diference-nitrogen level on the content of chlorophyl and carboxylase activity of RuBP and PEP in the leaf of spring maize during main growing period.[Result]Applying proper amount of N could keep relative higher content of chlorophyl and higher activity of carboxylase of RuBP and PEP in the leaf of spring maize,insufficient or excessive(N 400 kg/hm^2) of nitrogenous fertilizer has the adverse effect.[Conclusion]In this experiment,applying 300 kg/hm^2 amount of N could keep relative higher content of chlorophyl and higher activity of carboxylase of RuBP and PEP in the leaf of spring maize during main growing period.It was important to strengthens the leaf blade photosynthesis ability,promote the yield formation and postpone the decline of leaf blade. 展开更多
关键词 Nitrogen nutrition Spring maize carbon metabolism
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Response of leaf carbon metabolism and dry matter accumulation to density and row spacing in two rapeseed(Brassica napus L.)genotypes with differing plant architectures 被引量:6
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作者 Jie Kuai Xiaoyong Li +4 位作者 Jianli Ji Zhen Li Yan Xie Bo Wang Guangsheng Zhou 《The Crop Journal》 SCIE CSCD 2022年第3期680-691,共12页
Biological yield indicates the potential for increasing yield.Leaf carbon metabolism plays an important role in the biomass accumulation of rapeseed(Brassica napus L.).Field experiments with the hybrid HZ62(with a con... Biological yield indicates the potential for increasing yield.Leaf carbon metabolism plays an important role in the biomass accumulation of rapeseed(Brassica napus L.).Field experiments with the hybrid HZ62(with a conventional plant architecture)grown in 2016–2017,and HZ62 and accession 1301(with a compact plant architecture)grown in 2017–2018 were conducted to characterize the physiological and proteomic responses of leaf photosynthetic carbon metabolism to density and row spacing configurations.The densities were set at 15×10;ha^(-1)(D1),30×10^(4)ha^(-1)(D2),and 45×10^(4)ha^(-1)(D3)(main plot),with row spacings of 15 cm(R15),25 cm(R25),and 35 cm(R35)(subplot).Individual and plant population biomass accumulation was greatest at R25,R15,and R15 for D1,D2,and D3,respectively,for both genotypes.In comparison with D1 R25,the individual aboveground biomass of HZ62 decreased by60.2%,whereas the population biomass increased by 31.9%,and the individual biomass of genotype1301 decreased by 54.0%and the population biomass increased by 53.9%at D3 R15.Leaf carbon metabolic enzymes varied between genotypes at flowering stage.In contrast to D1 R25,at D3 R15 the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)and sucrose phosphate synthase(SPS)and the contents of starch,sucrose and soluble sugars in leaves were significantly decreased in HZ62 and increased in genotype 1301.The activities of fructose-1,6-bisphosphatase(FBPase)decreased,in consistency with the abundance of fructose-bisphosphate aldolase in HZ62.In contrast,sucrose synthase(Su Sy)activity appeared to decrease in both genotypes,but a significant increase in abundance of a protein with sucrose synthase was found in the 1301 genotype by proteomic analysis.With increased density and reduced row spacing,the expression of most key proteins involved in carbon metabolism was elevated,and enzyme activity and carbon assimilate content were increased in 1301,whereas HZ62 showed the opposite trend,indicating that the compact plant type can accumulate more population biomass with denser planting. 展开更多
关键词 RAPESEED DENSITY Row spacing Dry matter Leaf carbon metabolism
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Effects of Biological Bacterial Fertilizer on Carbon Metabolism Characteristics of Rhizosphere Soil Bacteria in Rice 被引量:3
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作者 ZHAO Jie ZHU Xiao-lei +2 位作者 YE Ming HE Xing-wu HUANG Wu-jian 《Agricultural Science & Technology》 CAS 2020年第3期25-29,共5页
The effects of biological bacterial fertilizer and chemical fertilizer on carbon metabolism characteristics of rhizosphere soil bacteria in rice were studied through a plot experiment.The results showed that the numbe... The effects of biological bacterial fertilizer and chemical fertilizer on carbon metabolism characteristics of rhizosphere soil bacteria in rice were studied through a plot experiment.The results showed that the number and Mcintosh index of bacteria in rice rhizosphere soil increased significantly with the application of biological bacterial fertilizer.It was found that the AWCD(average well color development)value of the bacteria remarkably increased and the decomposition of carboxylic acids,amines and heterozygotes were accelerated when adding biological bacterial fertilizer at a proper weight percent.All in all,proper addition of biological bacterial fertilizer could increase the number and carbon metabolism of bacteria.The most appropriate application rate was 70%chemical fertilizer nitrogen+30%biological bacterial fertilizer nitrogen for rice production in Northern Jiangsu Province. 展开更多
关键词 Biological bacterial fertilizer Rhizosphere soil BACTERIA carbon metabolism characteristics
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Effects of Drought Stress on Key Enzymes of Carbon Metabolism, Photosynthetic Characteristics and Agronomic Traits of Soybean at the Flowering Stage under Different Soil Substrates 被引量:1
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作者 Shuang Song Xiaomei Li +4 位作者 Xin Wang Qi Zhou Yongping Li Xiaojing Wang Shoukun Dong 《Phyton-International Journal of Experimental Botany》 SCIE 2022年第11期2475-2490,共16页
Soybean is an important legume food crop,and its seeds are rich in nutrients,providing humans and animals with edible oil and protein feed.However,soybean is sensitive to water requirements,and drought is an important... Soybean is an important legume food crop,and its seeds are rich in nutrients,providing humans and animals with edible oil and protein feed.However,soybean is sensitive to water requirements,and drought is an important factor limiting soybean yield and quality.This study used Heinong 84(drought resistant variety)and Hefeng 46(intermediate variety)as tested varieties planted in chernozem,albic,and black soils.The effects of drought stress on the activities of key enzymes in carbon metabolism and photosynthetic characteristics of soybean were studied during the flowering stage,most sensitive to water.(1)The activities of SS-1,6PGDH,and G6PDH enzymes in soybean leaves first increased and then decreased under drought stress.The enzyme activity was the highest under moderate drought stress and weakest in the blank group.(2)Drought stress increased Phi2,PhiNO,and Fm in soybean leaves and reached the highest value under severe drought;with the increase in drought stress,PhiNPQ and Fv/Fm of soybean leaves gradually decreased,reaching the lowest under severe drought.(3)With the increase in drought stress,F0 and Fs of soybean leaves showed a single peak curve,and the maximum was at moderate drought.(4)Correlation analysis showed that F0 was greatly affected by varieties and soil types;Fs,F0,and Fm soil varieties had a great influence,and chlorophyll fluorescence parameters were affected differently under drought stress with different drought degrees.(5)Drought stress changed the agronomic traits and yield of soybean.With the increase of drought degree,plant height,node number of main stem,effective pod number,100-seed weight and total yield decreased continuously.(6)Drought stress affected the dry matter accumulation of soybean.With the increase of drought degree,the dry matter accumulation gradually decreased.Among them,the leaf was most seriously affected by drought,and SD decreased by about 55%compared with CK.Under the condition of black soil,the dry matter accumulation of soybean was least affected by drought. 展开更多
关键词 SOYBEAN DROUGHT chlorophyll fluorescence parameters agronomic character key enzymes of carbon metabolism correlation analysis
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Carbon Metabolism and Bioplastic Production by Halophilic Archaea
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作者 XIANG Hua HAN Jing +3 位作者 HOU Jing ZHAO Dahe CAI Shuangfeng CAI Lei 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2014年第S1期116-118,共3页
1 Introduction Haloarchaea represents a distinct group of Archaea that typically inhabits hypersaline environments,such as salt lakes and sea salterns.They are easy to culture and many haloarchaea are genetically trac... 1 Introduction Haloarchaea represents a distinct group of Archaea that typically inhabits hypersaline environments,such as salt lakes and sea salterns.They are easy to culture and many haloarchaea are genetically tractable,hence they are excellent model systems for research of archaeal genetics, 展开更多
关键词 HALOARCHAEA carbon metabolism bioplastic production
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Synergistic effects of carbon cycle metabolism and photosynthesis in Chinese cabbage under salt stress 被引量:1
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作者 Hao Liang Qiling Shi +8 位作者 Xing Li Peipei Gao Daling Feng Xiaomeng Zhang Yin Lu Jingsen Yan Shuxing Shen Jianjun Zhao Wei Ma 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期461-472,共12页
Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and horm... Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress. 展开更多
关键词 Chinese cabbage Salt stress carbon metabolism PHOTOSYNTHESIS CHLOROPLAST
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Mepiquat chloride increases the Cry1Ac protein content of Bt cotton under high temperature and drought stress by regulating carbon and amino acid metabolism
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作者 Dian Jin Yuting Liu +7 位作者 Zhenyu Liu Yuyang Dai Jianing Du Run He Tianfan Wu Yuan Chen Dehua Chen Xiang Zhang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第12期4032-4045,共14页
The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt ... The effects of mepiquat chloride(DPC)on the Cry1Ac protein content in Bacillus thuringiensis(Bt)cotton boll shells under high temperature and drought stress were investigated to provide a theoretical reference for Bt cotton breeding and high-yield and-efficiency cotton cultivation.This study was conducted using Bt cotton cultivar‘Sikang 3'during the 2020 and 2021 growing seasons at Yangzhou University Farm,Yangzhou,Jiangsu Province,China.Potted cotton plants were exposed to high temperature and drought stress,and sprayed with either 20 mg L^(-1)DPC or water(CK).Seven days after treatment,the Cry1Ac protein content,α-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content were measured,and transcriptome sequencing was performed.DESeq was used for differential gene analysis.Under the DPC treatment,the Cry1Ac protein content increased by 4.7-11.9% compared to CK.Theα-ketoglutarate content,pyruvic acid content,glutamate synthase activity,glutamic oxaloacetic transaminase activity,soluble protein content,and amino acid content all increased.Transcriptome analysis revealed 7,542 upregulated genes and 10,449 downregulated genes for DPC vs.CK.Gene ontology(GO)and Kyoto Encyclopedia of Gene and Genomes(KEGG)analyses showed that the differentially expressed genes were mainly involved in biological processes,such as carbon and amino acid metabolism.For example,genes encoding 6-phosphofructokinase,pyruvate kinase,glutamic pyruvate transaminase,pyruvate dehydrogenase,citrate synthase,isocitrate dehydrogenase,2-oxoglutarate dehydrogenase,glutamate synthase,1-pyrroline-5-carboxylate dehydrogenase,glutamic oxaloacetic transaminase,amino-acid N-acetyltransferase,and acetylornithine deacetylase were all significantly upregulated.The DPC treatment increased pyruvate,α-ketoglutarate,and oxaloacetate by increasing the operational rate of the glycolytic pathway of the citric acid cycle.It also significantly upregulated the genes encoding glutamate synthase,pyrrolidine-5-carboxylic acid dehydrogenase,glutamate oxaloacetate transaminase,and N-acetylglutamate synthetase,while it downregulated the genes encoding glutamine synthetase.Therefore,the synthesis of aspartic acid,glutamic acid,pyruvate,and arginine increased after treatment with DPC,and the Cry1Ac protein content was increased by regulating carbon and amino acid metabolism. 展开更多
关键词 amino acid metabolism Bt cotton carbon metabolism Cry1Ac protein mepiquat chloride
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Community structure and carbon metabolism functions of bacterioplankton in the Guangdong coastal zone
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作者 Ziqi Peng Pandeng Wang +9 位作者 Xiaoqing Luo Qiqi Deng Ziwen Yang Jiaxue Wu Wendong Xian Weicong Yan Xiaozhen Mou Yang Yuan Wenjun Li Jialing Li 《Marine Life Science & Technology》 SCIE CSCD 2024年第3期547-561,共15页
Coastal ecosystems are an important region for biogeochemical cycling,are a hotspot of anthropogenic disturbance and play a crucial role in global carbon cycling through the metabolic activities of bacterioplankton.Ba... Coastal ecosystems are an important region for biogeochemical cycling,are a hotspot of anthropogenic disturbance and play a crucial role in global carbon cycling through the metabolic activities of bacterioplankton.Bacterioplankton can be broadly classified into two lifestyles:free-living(FL)and particle-attached(PA).However,how coastal bacterioplankton the community structure,co-occurrence networks and carbon metabolic functions with different lifestyles are differentiated is still largely unknown.Understanding these processes is necessary to better determine the contributions of coastal bacterioplankton to carbon cycling.Here,the characteristics of community structure and carbon metabolism function of bacterioplankton with two lifestyles in the coastal areas of Guangdong Province were investigated using amplicon sequencing,metagenomic,and metatranscriptomic techniques.The results show that the main bacterioplankton responsible for carbon metabolism were the Pseudomonadota,Bacteroidota,and Actinomycetota.The microbial community structure,carbon metabolic function,and environmental preferences differ between different lifestyles.FL and PA bacteria exhibited higher carbon fixation and degradation potentials,respectively.A range of environmental factors,such as dissolved oxygen,pH,and temperature,were associated with the community structure and carbon metabolic functions of the bacterioplankton.Human activities,such as nutrient discharge,may affect the distribution of functional genes and enhance the carbon degradation functions of bacterioplankton.In conclusion,this study increased the understanding of the role of microorganisms in regulating carbon export in coastal ecosystems with intense human activity. 展开更多
关键词 Particle-attached and free-living bacterioplankton Community structure carbon metabolism Coastal ecosystem
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Combined effect of bicarbonate and water in photosynthetic oxygen evolution and carbon neutrality 被引量:1
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作者 Yanyou Wu 《Acta Geochimica》 EI CAS CSCD 2023年第1期77-88,共12页
Carbon neutrality is widely concerned and highly valued by many countries.Biosphere has always maintained the balance between oxidized organic substances and assimilated organic matter,resulting in netzero carbon diox... Carbon neutrality is widely concerned and highly valued by many countries.Biosphere has always maintained the balance between oxidized organic substances and assimilated organic matter,resulting in netzero carbon dioxide(CO_(2)) emissions and maintaining its own carbon neutrality.Nature has set a good example for human beings to coordinate oxygen(O_(2)) balance and CO_(2)balance,and achieve carbon neutrality.How does photosynthetic oxygen evolution initiate carbon and water neutrality?My synthesis shows that photo system Ⅱ functions as carbonic anhydrase to catalyze the reaction of CO_(2)hydration under physiological conditions,and CO_(2)hydration coupled with chemical equilibrium,H^(+)+HCO_(3)^(-)→1/2O_(2)+2e^(-)+2H^(+)+CO_(2),occurs in a photosystem Ⅱ corecomplex.Meanwhile,I focused on the revisiting of four classical heavy oxygen(O^(18)) labeling experiments and found that bicarbonate can promote photo synthetic oxygen evolution,and that photo synthetic oxygen evolution can alternately come from bicarbonate and water,not only water.Bicarbonate photolysis and water photolysis account for half of the photo synthetic oxygen evolution respectively,which can well explain the bicarbonate effect,Dole effect and plants’ environmental adaptability.Photosynthetic oxygen evolution initiated the journey of water metabolism and carbon metabolism in nature,which led to the coupling as 1:1(mol/mol) stoichiometric relationship between the reduction of CO_(2)and oxidation of organic carbon,coordinated the evolution of the atmosphere,hydrosphere,lithosphere and biosphere,and realized "carbon neutrality" in the whole Earth system. 展开更多
关键词 Bicarbonate photolysis Bicarbonate effect carbonic anhydrase Dole effect Water photolysis Stoichiometric relationship carbon metabolism
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Response of growth,metabolism and yield of Dendrocalamopsis oldhami to long-day photoperiod and fertilizer compensation 被引量:2
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作者 Zixu Yin Shaohui Fan +5 位作者 Wen Xia Yang Zhou Xiao Zhou Xuan Zhang Chengji Li Fengying Guan 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第1期151-166,共16页
The effects of long-day photoperiod on growth,photo synthetic fluorescence,carbon and nitrogen metabolism,and yield of Dendrocalamopsis oldhami and the compensation effects of fertilization were investigated.A complet... The effects of long-day photoperiod on growth,photo synthetic fluorescence,carbon and nitrogen metabolism,and yield of Dendrocalamopsis oldhami and the compensation effects of fertilization were investigated.A completely randomized design was used with two light factors(bamboo culms cultivated in solar greenhouse under long-day[Ls]and short-day[Ln]treatments);two organic nitrogen fertilizer levels(application of organic fertilizer[OF]and no organic fertilizer[NF]);and three nitrogen fertilizer levels(Low[N0],medium[N1]and high nitrogen[N2]).Leaf chlorophyll and fluorescence parameters(φPo,PIABS,and ETo/CSm)decreased and DIo/CSmincreased in Ls compared to Ln.Indole acetic acid(IAA)and gibberellic acid(GA3)levels decreased,whereas abscisic acid(ABA)increased.Leaf area decreased and leaf dry mass increased.The contents of carbon and nitrogen metabolism-related enzymes(nitrate reductase,glutamine synthetase,amylase,and sucrose synthase)and products(total nitrogen,organic carbon,soluble sugar,and starch)increased.Single bamboo shoot weight and diameter at breast height decreased,whereas shoot quantity and total yield increased.Fertilizer application significantly affected physiological growth and yield in the two light treatments,thus promoting carbon and nitrogen metabolism.TheφPo,PIABS,IAA,and GA3contents increased slightly,whereas ABA levels decreased.Shoot quantity,individual weight,and total yield improved.IA A,soluble sugar,and total yield to organic manure and light were lower than those of nitrogen levels(FN>FL,FO).Other indicators showed lower responses to different fertilization treatments than the light factor(FL>FN,FO).The ability of D.oldhami to alter its morphological and physiobiochemical traits and yield in response to variations in light applications may translate into high phenotypic plasticity.Fertilization significantly improved photoplasticity of D.oldhami.Under Ls,D.oldhami had high metabolic rates.was easily inhibited by light,and showed accelerated leaf senescence,and shoot quantity and total output increased.However,the quality of individual shoots decreased.Different fertilization treatments affected D.oldhami differently under the two light intensities.Ls sensitivity to nitrogen was higher.Fertilization could delay leaf dormancy and senescence under Ls treatment.Organic fertilizer addition could improve yield more effectively,with OFN1being the optimal fertilization level. 展开更多
关键词 Light NITROGEN Organic fertilizer Fluorescence parameters carbon and nitrogen metabolism YIELD
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Effects of Sodium Nitroprussiate on the Metabolites and Key Enzyme Activities of Carbon-nitrogen Metabolism from Seed Germination to Seedling Period of Cherry Tomato
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作者 Yongfu ZHANG Lihong GUO +3 位作者 Shuping JIANG Song JIN Liyuan YAO Mingfang ZHAO 《Agricultural Biotechnology》 CAS 2018年第1期20-25,29,共7页
This study was conducted to elucidate the regulating mechanism of sodium nitroprussiate on seed germination and seedling growth of cherry tomato. After the treatment with different concentrations of sodium nitroprussi... This study was conducted to elucidate the regulating mechanism of sodium nitroprussiate on seed germination and seedling growth of cherry tomato. After the treatment with different concentrations of sodium nitroprusside, the effects on the metabolites and key enzyme activities in carbon-nitrogen metabolism of cherry tomato cuhivar Zhuyun as an experimental material were investigated. The results showed that from seed germination to seedling period, the contents of starch and total nitrogen decreased, but the contents of soluble sugar, reducing sugar, sucrose and soluble protein increased firstly and then decreased; and free amino acids content increased gradually. Sodium nitroprusside made the contents of starch, sucrose and free amino acids higher than CK. From seed germination to seedling period in cherry tomato, the activities of amylase, glutamine synthetase (GS) and sucrose phosphate synthase (SPS) decreased; the activities of nitrate reductase (NR) and glutamate synthase (GOGAT) increased at first and decreased then; and the CK and the 0.25 mmol/L sodium nitroprusside treatments exhibited de- creased sucrose synthase (SS) activity, and the trend was increasing at first and decreasing then after the treatment with 0.50 and 1.00 mmol/L sodium nitroprus- side. Sodium nitroprusside treatment improved amylase activity; and the carbon-nitrogen ratio of the CK increased at first and decreased then, while the values of other treatments tended to decrease. In addition, sodium nitroprusside did not affect seed germination potential and germination rate, but significantly improved biomass accumulation, root length and height of seedlings. These data suggest that sodium nitroprusside could affect the conversion of starch and sugar accumulation, delay the decomposition of total nitrogen and soluble protein, and achieve the effects of accelerating the accumulation of free amino acids, and promoting seed germination and seedling growth, and 0.50 mmol/L sodium nitroprusside has the best effect. 展开更多
关键词 Cherry tomatoes Sodium nitroprusside Seed germination SEEDLING carbon and nitrogen metabolism Key enzymes
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Effects of different doses of glucose and fructose on central metabolic pathways and intercellular wireless communication networks in humans
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作者 Dingqiang Lu Yujiao Liu +9 位作者 Miao Zhao Shuai Yuan Danyang Liu Xinqian Wang Yixuan Liu Yifei Zhang Ming Li Yufeng Lü Guangchang Pang Ruijuan Ren 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第4期1906-1916,共11页
Fructose and glucose are often widely used in food processing and may contribute to many metabolic diseases.To observe the effects of different doses of glucose and fructose on human metabolism and cellular communicat... Fructose and glucose are often widely used in food processing and may contribute to many metabolic diseases.To observe the effects of different doses of glucose and fructose on human metabolism and cellular communication,volunteers were given low,medium,and high doses of glucose and fructose.Serum cytokines,glucose,lactate,nicotinamide adenine dinucleotide(NADH)and metabolic enzymes were assayed,and central carbon metabolic pathway networks and cytokine communication networks were constructed.The results showed that the glucose and fructose groups basically maintained the trend of decreasing catabolism and increasing anabolism with increasing dose.Compared with glucose,low-dose fructose decreased catabolism and increased anabolism,significantly enhanced the expression of the inflammatory cytokine interferon-γ(IFN-γ),macrophage-derived chemokine(MDC),induced protein-10(IP-10),and eotaxin,and significantly reduced the activity of isocitrate dehydrogenase(ICDH)and pyruvate dehydrogenase complexes(PDHC).Both medium and high doses of fructose increase catabolism and anabolism,and there are more cytokines and enzymes with significant changes.Furthermore,multiple cytokines and enzymes show strong relevance to metabolic regulation by altering the transcription and expression of enzymes in central carbon metabolic pathways.Therefore,excessive intake of fructose should be reduced to avoid excessive inflammatory responses,allergic reactions and autoimmune diseases. 展开更多
关键词 FRUCTOSE GLUCOSE Central carbon metabolic pathway Metabolic enzymes Cytokine network
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Microbial community structure and functional metabolic diversity are associated with organic carbon availability in an agricultural soil 被引量:6
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作者 LI Juan LI Yan-ting +3 位作者 YANG Xiang-dong ZHANG Jian-jun LIN Zhi-an ZHAO Bing-qiang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第12期2500-2511,共12页
Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental ... Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included: a non-amended control(CK), a commonly used application rate of inorganic fertilizer treatment(NPK); a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment(NPKM), and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment(NPKS). Denaturing gradient gel electrophoresis(DGGE) of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term(NPKM, NPKS) significantly promoted soil bacterial structure than the application of inorganic fertilizer only(NPK), and NPKM treatment was the most important driver for increases in the soil microbial community richness(S) and structural diversity(H). Overall utilization of carbon sources by soil microbial communities(average well color development, AWCD) and microbial substrate utilization diversity and evenness indices(H' and E) indicated that long-term inorganic fertilizer with organic amendments incorporated(NPKM, NPKS) could significantly stimulate soil microbial metabolic activity and functional diversity relative to CK, while no differences of them were found between NPKS and NPK treatments. Principal component analysis(PCA) based on carbon source utilization profiles also showed significant separation of soil microbial community under long-term fertilization regimes and NPKM treatment was significantly separated from the other three treatments primarily according to the higher microbial utilization of carbohydrates, carboxylic acids, polymers, phenolic compounds, and amino acid, while higher utilization of amines/amides differed soil microbial community in NPKS treatment from those in the other three treatments. Redundancy analysis(RDA) indicated that soil organic carbon(SOC) availability, especially soil microbial biomass carbon(Cmic) and Cmic/SOC ratio are the key factors of soil environmental characteristics contributing to the increase of both soil microbial community structure and functional metabolic diversity in the long-term fertilization trial. Our results showed that long-term inorganic fertilizer and swine manure application could significantly improve soil bacterial community structure and soil microbial metabolic activity through the increases in SOC availability, which could provide insights into the sustainable management of China's soil resource. 展开更多
关键词 long-term fertilization regimes organic amendment soil microbial community structure microbial functional metabolic activity carbon substrate utilization
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Effects of Mg on C and N Metabolism of Soybean at Different Nitrogen Supplying Levels
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作者 LANG Man LIU Yuanying PENG Xianlong ZHANG Wenzhao 《Journal of Northeast Agricultural University(English Edition)》 CAS 2006年第1期31-35,共5页
A pot experiment was conducted to study the effects of magnesium on carbon and nitrogen metabolism of soybean at different nitrogen supplying levels. The results showed that the effects of magnesium at low nitrogen ra... A pot experiment was conducted to study the effects of magnesium on carbon and nitrogen metabolism of soybean at different nitrogen supplying levels. The results showed that the effects of magnesium at low nitrogen rate on N content, soluble protein, soluble sugar contents were not alike at different growth stage, although nodule dry weights raised, the yield and protein content of seeds decreased, however, the oil content was improved. The application of magnesium at medium and high nitrogen supplying levels promoted the uptake of N effectively, increased the soluble protein and soluble sugar contents, but the nodule dry weights of application magnesium at medium nitrogen supplying level decreased and the yield increased only a little despite the improved quality. Application of magnesium at high nitrogen supplying level raised nodule dry weights and soybean yield significantly, the quality of seeds was also improved. 展开更多
关键词 SOYBEAN magnesium(Mg) nitrogen supplying carbon and nitrogen metabolism
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Impact of dietary fat levels and fatty acid composition on milk fat synthesis in sows at peak lactation
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作者 Li Zhe Uffe Krogh +3 位作者 Charlotte Lauridsen Mette Olaf Nielsen Zhengfeng Fang Peter Kappel Theil 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第3期1134-1149,共16页
Background Dietary fat is important for energy provision and immune function of lactating sows and their progeny.However,knowledge on the impact of fat on mammary transcription of lipogenic genes,de novo fat synthesis... Background Dietary fat is important for energy provision and immune function of lactating sows and their progeny.However,knowledge on the impact of fat on mammary transcription of lipogenic genes,de novo fat synthesis,and milk fatty acid(FA)output is sparse in sows.This study aimed to evaluate impacts of dietary fat levels and FA composition on these traits in sows.Forty second-parity sows(Danish Landrace×Yorkshire)were assigned to 1 of 5 dietary treatments from d 108 of gestation until weaning(d 28 of lactation):low-fat control diet(3%added animal fat);or 1 of 4 high-fat diets with 8%added fat:coconut oil(CO),fish oil(FO),sunflower oil(SO),or 4%octanoic acid plus 4%FO(OFO).Three approaches were taken to estimate de novo milk fat synthesis from glucose and body fat.Results Daily intake of FA was lowest in low-fat sows within fat levels(P<0.01)and in OFO and FO sows within highfat diets(P<0.01).Daily milk outputs of fat,FA,energy,and FA-derived carbon reflected to a large extent the intake of those.On average,estimates for de novo fat synthesis were 82 or 194 g/d from glucose according to method 1 or 2 and 255 g de novo+mobilized FA/d according to method 3.The low-fat diet increased mammary FAS expression(P<0.05)and de novo fat synthesis(method 1;P=0.13)within fat levels.The OFO diet increased de novo fat synthesis(method 1;P<0.05)and numerically upregulated mammary FAS expression compared to the other high-fat diets.Across diets,a daily intake of 440 g digestible FA minimized milk fat originating from glucose and mobilized body fat.Conclusions Sows fed diets with low-fat or octanoic acid,through upregulating FAS expression,increased mammary de novo fat synthesis whereas the milk FA output remained low in sows fed the low-fat diet or high-fat OFO or FO diets,indicating that dietary FA intake,dietary fat level,and body fat mobilization in concert determine de novo fat synthesis,amount and profiles of FA in milk. 展开更多
关键词 carbon metabolism De novo fat synthesis Dietary fatty acid Fat balance Mammary gene expression Mammary lipogenesis Milk fat production Piglet growth
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Transcriptomic analysis of biofilm formation by Bacillus cereus under different carbon source conditions
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作者 祁亚劲 周建伟 +3 位作者 吴雨豪 韩敬增 刘东红 吕瑞玲 《Food Quality and Safety》 SCIE CAS CSCD 2024年第1期55-67,共13页
Background:Previous studies found differences in the utilization of different carbon sources during biofilm formation by Bacillus cereus.Illumina HiSeq high-throughput sequencing technology was used to investigate the... Background:Previous studies found differences in the utilization of different carbon sources during biofilm formation by Bacillus cereus.Illumina HiSeq high-throughput sequencing technology was used to investigate the changes in gene transcript levels in Bacillus cereus biofilm bacteria under different carbon source conditions.Results:Compared with the control group,the number of differentially expressed genes in the glucose,maltose,lactose,and skim milksupplemented groups was 351,1136,133,and 487,respectively.The results showed that the pathways involved in the differentially expressed genes were mainly distributed in glycolysis and pentose phosphate pathway,tricarboxylic acid cycle,amino acid metabolism,and fatty acid metabolism.The gene expression of enzymes related to acetoin synthesis from pyruvate was mostly upregulated in the glucose-supplemented group.The gene expression of enzymes related to pyruvate synthesis of branched-chain amino acids in the maltose-supplemented group was mostly upregulated.In the lactose-supplemented group,the gene expression of acetoin biosynthesis from pyruvate was upregulated.Pyruvate production through glycolysis pathway increased in the skim milk-supplemented group,but the metabolic capacity of the tricarboxylic acid cycle did not change significantly.Conclusion:The content of pyruvate stored by Bacillus cereus biofilm bacteria through glycolysis or pentose phosphate pathway increased,but the carbon flux into the tricarboxylic acid cycle did not increase,which suggested that carbon fluxes in the extracellular polysaccharide synthesis pathway of the biofilm may be increased,resulting in increased biofilm biomass formation. 展开更多
关键词 Bacterial biofilm Bacillus cereus carbon metabolism TRANSCRIPTOMICS
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Twice-split phosphorus application alleviates low-temperature impacts on wheat by improved spikelet development and setting 被引量:1
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作者 XU Hui HOU Kuo-yang +7 位作者 FANG Hao LIU Qian-qian WU Qiu LIN Fei-fei DENG Rui ZHANG Lin-jie CHEN Xiang LI Jin-cai 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第12期3667-3680,共14页
Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the an... Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the anther interval stage)has resulted in significant yield losses of winter wheat.This study attempts to develop an economical,feasible,and efficient cultivation technique for improving the low-temperature(LT)resistance of wheat by exploring the effects of twice-split phosphorus application(TSPA)on wheat antioxidant characteristics and carbon and nitrogen metabolism physiology under LT treatment at the anther interval stage using Yannong 19 as the experimental material.The treatments consisted of traditional phosphorus application and TSPA,followed by a-4℃ LT treatment and natural temperature(NT)control at the anther interval stage.Our analyses showed that,compared with the traditional application,the TSPA increased the net photosynthetic rate(P_(n)),stomatal conductance(Gs),and transpiration rate(T_(r))of leaves and reduced the intercellular carbon dioxide concentration(C_(i)).The activity of carbon and nitrogen metabolism enzymes in the young wheat spikes was also increased by the TSPA,which promoted the accumulation of soluble sugar(SS),sucrose(SUC),soluble protein(SP),and proline(Pro)in young wheat spike and reduced the toxicity of malondialdehyde(MDA).Due to the improved organic nutrition for reproductive development,the young wheat spikes exhibited enhanced LT resistance,which reduced the sterile spikelet number(SSN)per spike by 11.8%and increased the spikelet setting rate(SSR)and final yield by 6.0 and 8.4%,respectively,compared to the traditional application.The positive effects of split phosphorus application became more pronounced when the LT treatment was prolonged. 展开更多
关键词 optimizing phosphorus application low-temperature stress carbon and nitrogen metabolism young spike development WHEAT
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Metabolic diversity and seasonal variation of soil microbial communities in natural forested wetlands
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作者 Di Wu Qi Chi +3 位作者 Xin Sui Mengmeng Zhang Hongbai Jia Guangyu Sun 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第6期2619-2631,共13页
This study explores the effects of vegetation and season on soil microorganisms and enzymatic activity of different wetlands in a temperate climate.Microbial carbon metabolism diversity was assessed using community-le... This study explores the effects of vegetation and season on soil microorganisms and enzymatic activity of different wetlands in a temperate climate.Microbial carbon metabolism diversity was assessed using community-level physiological profiles(CLPP)with 31 different carbon substrates.CLPP indicated that significant interactions occur during carbon substrate metabolism of the microorganisms.Furthermore,the different types of vegetation present in the wetland ecosystem combined with the seasonal effects to influence microbial carbon metabolism and enzymatic activity.The most significant differences occurred to carbohydrates,carboxylic acids,and amino acids.The Mantel test confirmed positive correlations between soil enzymatic activities and microbial carbon metabolism.Soil microorganisms in Betula ovalifolia and Carex schmidtii wetlands used carbon substrates more efficiently in summer than those in other forested wetlands during other periods.Enzymatic activities also showed a similar trend as microbial carbon metabolism.The results demonstrate that microbial carbon metabolism patterns can be used as biological indicators in wetland ecological alterations due to vegetation type or to seasonal factors. 展开更多
关键词 Forested wetlands Community-level physiological profiles(CLPP) carbon metabolism diversity Soil enzymatic activity SEASONALITY
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Transcriptomic analysis of biofilm formation by Bacillus cereus under different carbon source conditions
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作者 祁亚劲 周建伟 +3 位作者 吴雨豪 韩敬增 刘东红 吕瑞玲 《Food Quality and Safety》 SCIE CAS CSCD 2023年第4期553-565,共13页
Background:Previous studies found differences in the utilization of different carbon sources during biofilm formation by Bacillus cereus.Illumina HiSeq high-throughput sequencing technology was used to investigate the... Background:Previous studies found differences in the utilization of different carbon sources during biofilm formation by Bacillus cereus.Illumina HiSeq high-throughput sequencing technology was used to investigate the changes in gene transcript levels in Bacillus cereus biofilm bacteria under different carbon source conditions.Results:Compared with the control group,the number of differentially expressed genes in the glucose,maltose,lactose,and skim milksupplemented groups was 351,1136,133,and 487,respectively.The results showed that the pathways involved in the differentially expressed genes were mainly distributed in glycolysis and pentose phosphate pathway,tricarboxylic acid cycle,amino acid metabolism,and fatty acid metabolism.The gene expression of enzymes related to acetoin synthesis from pyruvate was mostly upregulated in the glucose-supplemented group.The gene expression of enzymes related to pyruvate synthesis of branched-chain amino acids in the maltose-supplemented group was mostly upregulated.In the lactose-supplemented group,the gene expression of acetoin biosynthesis from pyruvate was upregulated.Pyruvate production through glycolysis pathway increased in the skim milk-supplemented group,but the metabolic capacity of the tricarboxylic acid cycle did not change significantly.Conclusion:The content of pyruvate stored by Bacillus cereus biofilm bacteria through glycolysis or pentose phosphate pathway increased,but the carbon flux into the tricarboxylic acid cycle did not increase,which suggested that carbon fluxes in the extracellular polysaccharide synthesis pathway of the biofilm may be increased,resulting in increased biofilm biomass formation. 展开更多
关键词 Bacterial biofilm Bacillus cereus carbon metabolism TRANSCRIPTOMICS
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