The effects of various toxic carbonyls such as malondialdehyde(MDA),a secondary product of lipid peroxidation,and other aldehydes on rheological parameters and their relationship with aging-associated alterations were...The effects of various toxic carbonyls such as malondialdehyde(MDA),a secondary product of lipid peroxidation,and other aldehydes on rheological parameters and their relationship with aging-associated alterations were studied.Both MDA and glutaraldehyde(Glu) in different concentrations significantly increase viscosity,plastic viscosity and yield stress of human plasma and erythrocyte suspensions.MDA(20 mmol/L) reduces sharply the typical fluorescence of proteins(excitation 280 nm/emission 350 nm),and produces age pigment-like fluorescence with a strong emission peak at 460 nm when excites at 395 nm by only being incubated for some hours.In contrast,Glu decreases merely the fluorescence of proteins without producing age pigment-like fluorescence.These data suggest interestingly that the MDA-induced gradual protein cross linking seems to form from different mechanisms compared to the fast rheological changes of blood materials which may take place either in acute and chronic diseases or during aging.On the other hand,MDA induces various deleterious alterations of erythrocytes whereas glutathione(GSH) inhibits the MDA-related carbonyl stress in a concentration-dependent manner.The results indicate that carbonyl-amino reaction exists in the blood widely and GSH has the ability to interrupt or reverse this reaction in a certain way.It implies that carbonyl stress may be one of the important factors in blood stasis and suggests a theoretical and practical approach in anti-stresses and anti-aging.展开更多
The induction of tumor carbonyl stress is reported to efficiently revert immune suppression in the tumor microenvironment and enhance cancer immunotherapy.However,low oxygen concentration due to inherent tumor hypoxia...The induction of tumor carbonyl stress is reported to efficiently revert immune suppression in the tumor microenvironment and enhance cancer immunotherapy.However,low oxygen concentration due to inherent tumor hypoxia limits its catalytic effect.Herein,an injectable thermosensitive hydrogel system(named APH)is developed for co-loading of near-infrared(NIR)aggregation-induced emission(AIE)nanoparticles and plasma amine oxidase(PAO)for boosting carbonyl stress and enhancing antitumor immunity.Upon 808 nm NIR laser irradiation,the AIE nanoparticles trigger a mild-temperature(around 45◦C)photothermal effect in the tumor site,which significantly relieves tumor hypoxia and promotes the catalytic effect of released PAO to inhibit the growth of Myeloid-derived suppressor cells.Remarkably,the synergistic therapeutic effect of APH is verified through a significant inhibitory effect on the distant tumor,enhanced immune memory,and effective suppression of postoperative recurrence,rechallenge,and metastasis.Overall,the combined effect of AIE-mediated photothermal therapy and carbonyl stress by APH upon NIR irradiation therapy can significantly activate cancer immunotherapy,making it a promising treatment approach for cancer treatment.展开更多
Foxtail millet (Setaria italica L.) is a drought-tolerant millet crop of arid and semi-arid regions. Aldo-keto reductases (AKRs) are significant part of plant defence mechanism, having an ability to confer multiple st...Foxtail millet (Setaria italica L.) is a drought-tolerant millet crop of arid and semi-arid regions. Aldo-keto reductases (AKRs) are significant part of plant defence mechanism, having an ability to confer multiple stress tolerance. In this study, AKR1 gene expression was studied in roots and leaves of foxtail millet subjected to different regimes of PEG- and NaCl-stress for seven days. The quantitative Real-time PCR expression analysis in both root and leaves showed upregulation of AKR1 gene during PEG and salt stress. A close correlation exits between expression of AKR1 gene and the rate of lipid peroxidation along with the retardation of growth. Tissue-specific differences were found in the AKR1 gene expression to the stress intensities studied. The reduction in root and shoot growth under both stress conditions were dependent on stress severity. The level of lipid peroxidation as indicated by MDA formation was significantly increased in roots and leaves along with increased stress levels. Finally, these findings support the early responsive nature of AKR1 gene and seem to be associated at least in part with its ability to contribute in antioxidant defence related pathways which could provide a better protection against oxidative stress under stress conditions.展开更多
Oxidative stress is considered a central factor in endothelial dysfunction and plaque formation in coronary heart disease (CHD) patients. In this cross-?sectional study, the stress-induced protein and lipid oxidation ...Oxidative stress is considered a central factor in endothelial dysfunction and plaque formation in coronary heart disease (CHD) patients. In this cross-?sectional study, the stress-induced protein and lipid oxidation and production of signaling molecule, nitric oxide has been investigated in CHD patients in Bangladesh. A total of 140 participants were enrolled of which 70 were CHD patients, and the remaining 70 were control subjects having no cardiovascular disease. The diagnosis of CHD was made by expert physicians through characteristic electrocardiogram and troponin changes. The protein carbonyls and thiobarbituric acid reactive substances (TBARS) levels in plasma of both groups were measured by spectrophotometric methods. The plasma nitric oxide levels were measured by reaction with Griess reagent. Statistical non-parametric tests and t-tests were used to analyze the results between the CHD patients and controls. In the patients, the mean protein carbonyls and TBARS, two important markers of oxidative stress, were 2.87 ± 0.97 nmol/mg and 6.92 ± 3.56 nmol/mL, respectively, which were significantly higher than in the control subjects, 1.71 ± 0.82 nmol/mg and 3.15 ± 1.42 nmol/mL, respectively. Assessment of the cardiovascular signaling molecule nitric oxide showed significantly lower value in the CHD patients (6.29 ± 4.78 μM) compared to the controls (10.75 ± 6.03 μM). There was a significant negative correlation between plasma TBARS and total plasma protein, and a weak negative correlation between TBARS and nitric oxide levels in the CHD patients. Evaluation of the smoking habit strongly suggested that smoking was a risk factor for the development of CHD among the Bangladeshi population. These results provide an overview of oxidative?stress-mediated damages on proteins, lipids and effects on signaling molecule nitric oxide in CHD patients.展开更多
Oxidative stress, a pervasive condition of increased number of reactive oxygen species, is now recognized to be prominent feature of various diseases and their progression. The relationship between antioxidants and le...Oxidative stress, a pervasive condition of increased number of reactive oxygen species, is now recognized to be prominent feature of various diseases and their progression. The relationship between antioxidants and levels of well-known markers of oxidative stress, measured as lipid peroxides and oxidized proteins reflect health indices. The aim of this study is to evaluate the extent of oxidative stress and antioxidant status in acute and chronic myeloid leukemia patients. The present study included 60 patients selected using standard questionnaire based on age, family history, Body Mass Index (BMI), dietary intake, with no other complications and 30 age and sex-matched healthy subjects. The median age of myeloid leukemia patients was 43 years and that of controls was 42 years. Out of 60 myeloid leukemia patients, 30 were in acute and 30 were in chronic state. Oxidative stress and antioxidant status were evaluated in the patients and in the controls by assessing standard oxidative stress markers viz. plasma and erythrocyte lipid peroxide levels in terms of malondialdehyde and oxidized proteins as protein carbonyls whereas antioxidant status was assessed in terms of serum non enzymatic antioxidant levels. There was a significant increase (p 0.01) in plasma and erythrocyte lipid peroxidation and protein oxidation in acute and chronic myeloid leukemia patients as compared to healthy subjects. Antioxidant status as indicated by the levels of non-enzymatic antioxidants viz. erythrocyte reduced glutathione (GSH), serum β carotene, vitamin A & C and ceruloplasmin was found to be significantly decreased (p 0.01) in both the leukemia patients as compared to healthy participants. However, chronic myeloid leukemia patients had significantly (p 0.05) higher oxidative stress and lower antioxidant status as compared to acute myeloid leukemia patients.展开更多
基金Project(2007AA02Z433) supported by the National High Technology Research and Development Program of ChinaProject(2007RS4017) supported by the Postdoctoral Science Foundation of Hunan Province,China
文摘The effects of various toxic carbonyls such as malondialdehyde(MDA),a secondary product of lipid peroxidation,and other aldehydes on rheological parameters and their relationship with aging-associated alterations were studied.Both MDA and glutaraldehyde(Glu) in different concentrations significantly increase viscosity,plastic viscosity and yield stress of human plasma and erythrocyte suspensions.MDA(20 mmol/L) reduces sharply the typical fluorescence of proteins(excitation 280 nm/emission 350 nm),and produces age pigment-like fluorescence with a strong emission peak at 460 nm when excites at 395 nm by only being incubated for some hours.In contrast,Glu decreases merely the fluorescence of proteins without producing age pigment-like fluorescence.These data suggest interestingly that the MDA-induced gradual protein cross linking seems to form from different mechanisms compared to the fast rheological changes of blood materials which may take place either in acute and chronic diseases or during aging.On the other hand,MDA induces various deleterious alterations of erythrocytes whereas glutathione(GSH) inhibits the MDA-related carbonyl stress in a concentration-dependent manner.The results indicate that carbonyl-amino reaction exists in the blood widely and GSH has the ability to interrupt or reverse this reaction in a certain way.It implies that carbonyl stress may be one of the important factors in blood stasis and suggests a theoretical and practical approach in anti-stresses and anti-aging.
基金National Natural Science Foundation of China,Grant/Award Number:82002779Guangxi Natural Science Foundation,Grant/Award Number:2023GXNSFBA026137China Postdoctoral Science Foundation,Grant/Award Number:2022M710853。
文摘The induction of tumor carbonyl stress is reported to efficiently revert immune suppression in the tumor microenvironment and enhance cancer immunotherapy.However,low oxygen concentration due to inherent tumor hypoxia limits its catalytic effect.Herein,an injectable thermosensitive hydrogel system(named APH)is developed for co-loading of near-infrared(NIR)aggregation-induced emission(AIE)nanoparticles and plasma amine oxidase(PAO)for boosting carbonyl stress and enhancing antitumor immunity.Upon 808 nm NIR laser irradiation,the AIE nanoparticles trigger a mild-temperature(around 45◦C)photothermal effect in the tumor site,which significantly relieves tumor hypoxia and promotes the catalytic effect of released PAO to inhibit the growth of Myeloid-derived suppressor cells.Remarkably,the synergistic therapeutic effect of APH is verified through a significant inhibitory effect on the distant tumor,enhanced immune memory,and effective suppression of postoperative recurrence,rechallenge,and metastasis.Overall,the combined effect of AIE-mediated photothermal therapy and carbonyl stress by APH upon NIR irradiation therapy can significantly activate cancer immunotherapy,making it a promising treatment approach for cancer treatment.
文摘Foxtail millet (Setaria italica L.) is a drought-tolerant millet crop of arid and semi-arid regions. Aldo-keto reductases (AKRs) are significant part of plant defence mechanism, having an ability to confer multiple stress tolerance. In this study, AKR1 gene expression was studied in roots and leaves of foxtail millet subjected to different regimes of PEG- and NaCl-stress for seven days. The quantitative Real-time PCR expression analysis in both root and leaves showed upregulation of AKR1 gene during PEG and salt stress. A close correlation exits between expression of AKR1 gene and the rate of lipid peroxidation along with the retardation of growth. Tissue-specific differences were found in the AKR1 gene expression to the stress intensities studied. The reduction in root and shoot growth under both stress conditions were dependent on stress severity. The level of lipid peroxidation as indicated by MDA formation was significantly increased in roots and leaves along with increased stress levels. Finally, these findings support the early responsive nature of AKR1 gene and seem to be associated at least in part with its ability to contribute in antioxidant defence related pathways which could provide a better protection against oxidative stress under stress conditions.
文摘Oxidative stress is considered a central factor in endothelial dysfunction and plaque formation in coronary heart disease (CHD) patients. In this cross-?sectional study, the stress-induced protein and lipid oxidation and production of signaling molecule, nitric oxide has been investigated in CHD patients in Bangladesh. A total of 140 participants were enrolled of which 70 were CHD patients, and the remaining 70 were control subjects having no cardiovascular disease. The diagnosis of CHD was made by expert physicians through characteristic electrocardiogram and troponin changes. The protein carbonyls and thiobarbituric acid reactive substances (TBARS) levels in plasma of both groups were measured by spectrophotometric methods. The plasma nitric oxide levels were measured by reaction with Griess reagent. Statistical non-parametric tests and t-tests were used to analyze the results between the CHD patients and controls. In the patients, the mean protein carbonyls and TBARS, two important markers of oxidative stress, were 2.87 ± 0.97 nmol/mg and 6.92 ± 3.56 nmol/mL, respectively, which were significantly higher than in the control subjects, 1.71 ± 0.82 nmol/mg and 3.15 ± 1.42 nmol/mL, respectively. Assessment of the cardiovascular signaling molecule nitric oxide showed significantly lower value in the CHD patients (6.29 ± 4.78 μM) compared to the controls (10.75 ± 6.03 μM). There was a significant negative correlation between plasma TBARS and total plasma protein, and a weak negative correlation between TBARS and nitric oxide levels in the CHD patients. Evaluation of the smoking habit strongly suggested that smoking was a risk factor for the development of CHD among the Bangladeshi population. These results provide an overview of oxidative?stress-mediated damages on proteins, lipids and effects on signaling molecule nitric oxide in CHD patients.
文摘Oxidative stress, a pervasive condition of increased number of reactive oxygen species, is now recognized to be prominent feature of various diseases and their progression. The relationship between antioxidants and levels of well-known markers of oxidative stress, measured as lipid peroxides and oxidized proteins reflect health indices. The aim of this study is to evaluate the extent of oxidative stress and antioxidant status in acute and chronic myeloid leukemia patients. The present study included 60 patients selected using standard questionnaire based on age, family history, Body Mass Index (BMI), dietary intake, with no other complications and 30 age and sex-matched healthy subjects. The median age of myeloid leukemia patients was 43 years and that of controls was 42 years. Out of 60 myeloid leukemia patients, 30 were in acute and 30 were in chronic state. Oxidative stress and antioxidant status were evaluated in the patients and in the controls by assessing standard oxidative stress markers viz. plasma and erythrocyte lipid peroxide levels in terms of malondialdehyde and oxidized proteins as protein carbonyls whereas antioxidant status was assessed in terms of serum non enzymatic antioxidant levels. There was a significant increase (p 0.01) in plasma and erythrocyte lipid peroxidation and protein oxidation in acute and chronic myeloid leukemia patients as compared to healthy subjects. Antioxidant status as indicated by the levels of non-enzymatic antioxidants viz. erythrocyte reduced glutathione (GSH), serum β carotene, vitamin A & C and ceruloplasmin was found to be significantly decreased (p 0.01) in both the leukemia patients as compared to healthy participants. However, chronic myeloid leukemia patients had significantly (p 0.05) higher oxidative stress and lower antioxidant status as compared to acute myeloid leukemia patients.
