Myopathological features in MELAS syndrome without significant changes in muscle biopsy pathology

Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes （MELAS） are common types of mitochondrial encephalomyopathy. The involved muscular pathology is characterized by typical changes of mitochondrial abnormalities. Gene screening has been the gold diagnostic standard for MELAS diagnosis. This study presents three primary MELAS patients, with an age of onset from 13 to 18 years, including one patient with seizure, and two with headache and vomiting. All patients had a family history of disease, with maternal inheritance. Cerebral magnetic resonance imaging revealed abnormally high signals in T2-weighted images： temporal lobe in three cases, occipital lobe in two cases, and parietal lobe in one case. Migrating stroke-like lesions were confirmed in one patient. Muscle biopsy revealed several strongly succinate dehydrogenase-reactive vessels scattered in muscle sections of three patients, but ragged-red fibers and cytochrome c oxidase-negative/dense （COX-/＋） fibers were not observed. Mitochondrial DNA A3243G mutation was identified in all three cases. MELAS syndrome has obvious clinical heterogeneity, and muscle weakness was not prominent in some of the cases. Muscle pathological changes did not accompany ragged-red fibers or COX-/＋ fibers, but succinate dehydrogenase- reactive vessels are important for MELAS diagnosis.

Sonographic muscle mass assessment in patients after cardiac surgery

BACKGROUND Patients undergoing cardiac surgery particularly those with comorbidities and frailty,experience frequently higher rates of post-operative morbidity,mortality and prolonged hospital length of stay.Muscle mass wasting seems to play important role in prolonged mechanical ventilation(MV)and consequently in intensive care unit(ICU)and hospital stay.AIM To investigate the clinical value of skeletal muscle mass assessed by ultrasound early after cardiac surgery in terms of duration of MV and ICU length of stay.METHODS In this observational study,we enrolled consecutively all patients,following their admission in the Cardiac Surgery ICU within 24 h of cardiac surgery.Bedside ultrasound scans,for the assessment of quadriceps muscle thickness,were performed at baseline and every 48 h for seven days or until ICU discharge.Muscle strength was also evaluated in parallel,using the Medical Research Council(MRC)scale.RESULTS Of the total 221 patients enrolled,ultrasound scans and muscle strength assessment were finally performed in 165 patients(patients excluded if ICU stay<24 h).The muscle thickness of rectus femoris(RF),was slightly decreased by 2.2%[(95%confidence interval(CI):-0.21 to 0.15),n=9;P=0.729]and the combined muscle thickness of the vastus intermedius(VI)and RF decreased by 3.5%[(95%CI:-0.4 to 0.22),n=9;P=0.530].Patients whose combined VI and RF muscle thickness was below the recorded median values(2.5 cm)on day 1(n=80),stayed longer in the ICU(47±74 h vs 28±45 h,P=0.02)and remained mechanically ventilated more(17±9 h vs 14±9 h,P=0.05).Moreover,patients with MRC score≤48 on day 3(n=7),required prolonged MV support compared to patients with MRC score≥49(n=33),(44±14 h vs 19±9 h,P=0.006)and had a longer duration of extracorporeal circulation was(159±91 min vs 112±71 min,P=0.025).CONCLUSION Skeletal quadriceps muscle thickness assessed by ultrasound shows a trend to a decrease in patients after cardiac surgery post-ICU admission and is associated with prolonged duration of MV and ICU length of stay.

Investigation of the Distribution and Changes of VLDLR Subtype in Fibrotic Cardiac Muscles

Very low-density lipoprotein receptor (VLDLR) is the major receptor with which cells can uptake the triacylglycerol from blood. It is divided into two subtypes according to presence of O- linked sugar domain located in the VLDLR receptor immediately outside of the membrane. Type Ⅰ VLDLR contains the O-link domain, while type Ⅱhas no such domain. The type ⅠVLDLR are mainly found on the surface of human myocardial cells. The result of our quantitative polymerase chain reaction on the normal and fibrotic cardiac muscles showed that both subtypes and expression level of VLDLR on the myocardial cell surface did not vary significantly between the normal and the fibrotic cardiac muscles despite the presence of malfunction due to fibrosis. This finding suggests that fibrosis doesn't exert significant influence on the subtype and the expression of VLDLR on the sur- face of myocardial cells. Such inconsistence with the changes found in other fibrotic tissues is awaiting further studies.

Effect of preoperative inspiratory muscle training on postoperative outcomes in patients undergoing cardiac surgery:A systematic review and meta-analysis

BACKGROUND Cardiovascular disease is the most prevalent disease worldwide and places a great burden on the health and economic welfare of patients.Cardiac surgery is an important way to treat cardiovascular disease,but it can prolong mechanical ventilation time,intensive care unit(ICU)stay,and postoperative hospitalization for patients.Previous studies have demonstrated that preoperative inspiratory muscle training could decrease the incidence of postoperative pulmonary complications.AIM To explore the effect of preoperative inspiratory muscle training on mechanical ventilation time,length of ICU stay,and duration of postoperative hospitalization after cardiac surgery.METHODS A literature search of PubMed,Web of Science,Cochrane Library,EMBASE,China National Knowledge Infrastructure,WanFang,and the China Science and Technology journal VIP database was performed on April 13,2022.The data was independently extracted by two authors.The inclusion criteria were:(1)Randomized controlled trial;(2)Accessible as a full paper;(3)Patients who received cardiac surgery;(4)Preoperative inspiratory muscle training was implemented in these patients;(5)The study reported at least one of the following:Mechanical ventilation time,length of ICU stay,and/or duration of postoperative hospitalization;and(6)In English language.RESULTS We analyzed six randomized controlled trials with a total of 925 participants.The pooled mean difference of mechanical ventilation time was-0.45 h[95%confidence interval(CI):-1.59-0.69],which was not statistically significant between the intervention group and the control group.The pooled mean difference of length of ICU stay was 0.44 h(95%CI:-0.58-1.45).The pooled mean difference of postoperative hospitalization was-1.77 d in the intervention group vs the control group[95%CI:-2.41-(-1.12)].CONCLUSION Preoperative inspiratory muscle training may decrease the duration of postoperative hospitalization for patients undergoing cardiac surgery.More high-quality studies are needed to confirm our conclusion.

Effect of Cerium on Cardiac Muscle of Rat and Guinea Pig

The effect of Ce3+ on cardiac muscle of rat and guinea pig was studied. In vitro, 0.05 mmol·L-1 solution of Ce3+ inhibited the contraction of guinea pig atria. The change of action potential duration(APD) of guinea pig papillary muscle exposed to 0.4 mmol·L-1 Ce3+ was significant, and those exposed to 0.1 and 0. 2 mmol·L-1 Ce3+ were not significant. In vivo, compared with the control group, the APD for rat cardiac muscle after long-term feed on Ce3+ was significantly delayed in high dose, and that was not significantly delayed in low dose. The results suggest that Ce3+ with long-term high dose intake might affect the influx of Ca2+, Na+ and outflow of K+ for rat cardiac muscle.

Effect of Hypoxia on Ca^(2+) Concentration in Broiler's Cardiac Muscle Cells

The purpose of this research was to study the effect of hypoxia on the Ca^2＋ concentration in broiler＇s cardiac muscle cells （CMCs）. The concentration of Ca^2＋ in the CMC was observed using a laser scanning confocal microscope （LSCM）. The results showed that hypoxia could significantly increase intracellular Ca^2＋（normal oxygen, 99.3 ＋_ 13.1; hypoxia, 129.4 ＋_ 24.3, P 〈 0.01） in CMCs. The Ca^2＋ antagonist （nifedipine, verapamil） could significantly restrain the Ca^2＋ influx across the cell membrane of CMC treated by hypoxia （CMC： hypoxia ＋ verapamil, 100.9± 28.2; hypoxia ＋ nifedipine, 107.6± 27.7; P 〈 0.01）. The results showed hypoxia could increase intracellular Ca^2＋ concentration of CMC, and the Ca^2＋ antagonist could restrain the Ca^2＋ influx across the cell membrane of CMC treated by hypoxia.

The Effect of Fructose-1,6-diphosphate and HTK Solution on Protecting Primary Cardiac Muscle Cells of Rat with Cold Preservation

In this study we tried to investigate the effect of fructose-1,6-diphosphate and HTK solution on protecting primary cardiac muscle cells of rat with cold preservation. The primary cardiac muscle cells of rat were cultured in vitro with four preservation solutions respectively: 0.9 % sodium chloride solution (group A), FDP (group B), HTK solution (group C) and a mixture of FDP and HTK solution (group D). The cells were preserved for 6, 8 and 10 h at 0-4 ℃. The values of AST and LDH-L and the Na+-K+ ATPase activity in cardiac muscle cells were detected, and the survival rate of cardiac muscle cells was detected with trypan blue staining. The values of AST and LDH-L in group C and group D were remarkable lower those in group A and group B (P<0.001), while the Na+-K+ ATPase activity and the survival rate of cells in group C and group D were much higher than those in group A and group B (P<0.001). The values of AST and LDH-L after 6 hours in group D decreased much more than those in group C (P<0.01), while the Na+-K+ ATPase activity and the survival rate of cells in group D improved more than those in group C (P<0.01). Both of the HTK solution and the mixture of HTK and FDP solution have an evident effect on protecting the primary cardiac muscle cells of rat in vitro with cold preservation, Compared with the HTK solution, the mixture solution has a better short-term protective effect.

Pathological Characteristics of Liver Allografts from Donation after Brain Death Followed by Cardiac Death in Pigs

Donation after brain death followed by circulatory death （DBCD） is a unique practice in China. The aim of this study was to define the pathologic characteristics of DBCD liver allografts in a porcine model. Fifteen male pigs （25-30 kg） were allocated randomly into donation after brain death （DBD）, donation after circulatory death （DCD） and DBCD groups. Brain death was induced by aug- menting intracranial pressure. Circulatory death was induced by withdrawal of life support in DBCD group and by venous injection of 40 mL 10% potassium chloride in DCD group. The donor livers were perfused in situ and kept in cold storage for 4 h. Liver tissue and common bile duct samples were col- lected for hematoxylin and eosin staining, TUNEL testing and electron microscopic examination. Spot necrosis was found in hepatic parenchyma of DBD and DBCD groups, while a large area of necrosis was shown in DCD group. The apoptosis rate of hepatocytes in DBD [（0.56±0.30）%] and DBCD [（0.50 ±0.11）%] groups was much lower than that in DCD group [（3.78±0.33）%] （P〈0.05）. And there was no significant difference between DBD group and DBCD group （P〉0.05））. The structures of bile duct were intact in both DBD and DBCD groups, while the biliary epithelium was totally damaged in DCD group. Under electron microscope, the DBD hepatocytes were characterized by intact cell membrane, well-organized endoplasmic reticulum, mild mitochondria edema and abundant glycogens. Broken cell membrane, mild inflammatory cell infiltration and sinusoidal epithelium edema, as well as reduced glycogen volume, were found in the DBCD hepatocytes. The DCD hepatocytes had more profound cell organelle injury and much less glycogen storage. In conclusion, the preservation injury of DBCD liver allografts is much less severe than that of un-controlled DCD, but more severe than that of DBD liver allografts under electron microscope, which might reflect post-transplant liver function to some extent.

Metastatic low-grade endometrial stromal sarcoma with sex cord and smooth muscle differentiation: A case report

BACKGROUND Metastatic low-grade endometrial stromal sarcoma(LG-ESS) with sex cord-like and smooth muscle-like differentiation is rare. This article reports such a case with multiple recurrences and with extensive pelvic and abdominal metastasis.CASE SUMMARY A 47-year-old female patient was diagnosed with multiple cystic masses in the pelvic cavity by magnetic resonance imaging examination. Based on the postoperative pathological and immunohistochemical analyses of the surgical specimen, she was diagnosed with a metastatic low-grade endometrial stromal sarcoma with sex cord and smooth muscle differentiation.CONCLUSION LG-ESS is a low-grade malignant tumor with a high recurrence rate and metastasis probability. It is easily misdiagnosed initially. It is essential to distinguish LG-ESS with sex cord-like differentiation from uterine tumour resembling ovarian sex cord tumour.

Skeletal muscle metastases of hepatocellular carcinoma: A case report and literature review

BACKGROUND The metastasis of liver cancer to skeletal muscle is extremely rare compared to other sites.We herein report a case of rapidly developing skeletal metastases following liver transplantation due to primary liver cancer.CASE SUMMARY A 70-year-old male with underlying chronic hepatitis B virus infection was diagnosed with hepatocellular carcinoma(HCC),for which he underwent liver transplantation in 2014.Six years after receiving the transplant,pathological examination confirmed the presence of HCC without vascular invasion.He was admitted to the hospital with a rapidly growing mass on his right thigh.Ultrasound examination revealed a mixed echo mass in the lateral soft tissue of the middle part of the right femur.Magnetic resonance imaging showed heterogeneous iso-signal intensity on T1-weighted images and heterogeneous hyper-intensity on T2-weighted images compared to the surrounding muscles.Pathological examination of the ultrasound-guided needle biopsy specimen revealed that it was similar to the previously detected liver cancer;the diagnosis was metastasis of HCC.Surgical excision was performed.There were no other sites of metastasis,and the patient recovered well after surgery.CONCLUSION This report presents a rare case of skeletal metastasis following liver transplantation for HCC.The study suggests a possible role for skeletal muscle metastasis mechanisms,which should be the focus of future research.

Time-dependent Appearances of Myofibroblasts during the Re- pair of Contused Skeletal Muscle in Rat and Its Application for Wound Age Determination

Objective To research the relation between the time-dependent appearances of myofibroblasts during the repair of contused skeletal muscle in rat and wound age determination. Methods A total of 35 SD male rats were divided into the control and six injured groups according to wound age as follows: 12 h, 1 d, 5 d, 7 d, 10 d and 14 d after injury. The appearances of myofibroblasts were detected by HE staining, immunohistochemistry and confocal laser scanning microscopy. Masson's trichrome staining was utilized to examine collagen accumulation in the contused areas. Results Immunohistochemical staining showed that α-SMA+myofibroblasts were initially observed at 5 d post-injury. The average ratio of myofibroblasts was highest at 14 d post-injury, with all samples, ratios more than 50%. In the other five groups, the average of α-SMA positive ratios were less than 50%. The collagen stained areas in the contused zones, concomitant with myofibroblast appearance, were increasingly augmented along with advances of posttraumatic interval. Conclusion The immunohistochemical detection of myofibroblasts can be applied to wound age determination. The myofibroblasts might be involved in collagen deposition during the repair of contused skeletal muscle in rat.

Rbfox1 controls alternative splicing of focal adhesion genes in cardiac muscle cells

Alternative splicing is one of the major cellular processes that determine the tissue-specific expression of protein variants.However,it remains challenging to identify physiologically relevant and tissue-selective proteins that are generated by alternative splicing.Hence,we investigated the target spectrum of the splicing factor Rbfox1 in the cardiac muscle context in more detail.By using a combination of in silico target prediction and in-cell validation,we identified several focal adhesion proteins as alternative splicing targets of Rbfox1.We focused on the alternative splicing patterns of vinculin(metavinculin isoform)and paxillin(extended paxillin isoform)and identified both as potential Rbfox1 targets.Minigene analyses suggested that both isoforms are promoted by Rbfox1 due to binding in the introns.Focal adhesions play an important role in the cardiac muscle context,since they mainly influence cell shape,cytoskeletal organization,and cell–matrix association.Our data confirmed that depletion of Rbfox1 changed cardiomyoblast morphology,cytoskeletal organization,and multinuclearity after differentiation,which might be due to changes in alternative splicing of focal adhesion proteins.Hence,our results indicate that Rbfox1 promotes alternative splicing of focal adhesion genes in cardiac muscle cells,which might contribute to heart disease progression,where downregulation of Rbfox1 is frequently observed.

Cardiac mass in a patient with colorectal cancer:“Not all that glitters is gold!”

When performing echocardiography in a 74-year-old woman admitted with non ST elevation myocardial infarction and concomitant colorectal cancer(CC),a dense calcification of the mitral annulus was detected. Differential diagnosis between secondary metastasis and other etiologies of cardiac masses was essential for staging and therapeutic decision-making.Multimodality imaging with echocardiography alongside a computed tomography scan and cardiac magnetic resonance was crucial for the final diagnosis of caseous calcification of the mitral annulus(CCMA).CCMA is briefly reviewed and some possible explanations for the previously undescribed association of CC with CCMA are suggested.

Detection of Regional Wall Motion Abnormalities in Compressed Sensing Cardiac Cine Imaging

Background: Recently faster cardiac magnetic resonance (CMR) cine sequences basing on k-t compressed sensing have been developed. Purpose: To compare two compressed sensing CMR sequences-one in breath-hold technique and one during free breathing—with the standard SSFP sequence with respect to regional left ventricular function assessment. Material and Methods: Left ventricular short-axis stacks of two compressed sensing sequences in breath-hold technique (sparse_HB) and during free breathing (sparse_FB;both spatial resolution, 1.8 × 1.8 × 8 mm3) and a standard SSFP cine sequence (spatial resolution, 1.9 × 1.9 × 8 mm3) were acquired in 50 patients on a 1.5 T MR system. Regional wall motion abnormalities (RWMA) were rated qualitatively (normal/hypo-/a-/dyskinesia) by two experienced readers in consensus for all cardiac segments (American Heart Association’s segment model) and sequences. RWMA detection rates were compared between sequences by kappa statistic. Results: In 13 patients, RWMA were detected in at least one cardiac segment. The RWMA detection rates were similar between CMR sequences (hypokinesia, 7.2% to 7.9%;akinesia, 0.8% to 1.3%;dyskinesia 0.3% to 0.4%) and kappa statistics revealed an almost perfect agreement in RWMA detection between both sparse and the standard SSFP sequence (standard versus sparse_HB: kappa, 0.918, p value, p value, Conclusion: Compressed sensing cine CMR acquired during breath-hold or free-breathing allows reliable RWMA detection, thus, might alternatively be used in cine CMR for regional left ventricular function assessment.

Histopathological Evaluation of the Cardiotoxicity of Dihydroartemisinin-Piperaquine on Male Albino Rats

Problem Statement: Malaria’s global impact necessitates effective treatments, like dihydroartemisinin-piperaquine (DHA/PQP), though safety concerns, notably drug-induced cardiotoxicity (DICT), persist. A knowledge gap exists regarding DHA/PQP’s cardiac effects, warranting a comprehensive investigation. Approach: This study aimed to assess KROSH (DHA/PQP) impact on albino rat heart histology, examining structural changes and potential cardiotoxicity. 40 albino rats were grouped by KROSH dosage and duration, monitored for weight changes, and heart tissues were examined using hematoxylin and eosin (H & E) staining. Statistical analysis compared to control and treated groups. Results: KROSH administration led to varying rat weight effects, yet not statistically significant. Histological analysis revealed dose and duration-dependent cardiac tissue alterations, including distortion, adipose deposits, artery hypertrophy, fibrosis, and necrosis. These contrasts with prior research documenting DHA/PQP’s non-toxic effects. Conclusion/Recommendation: This study highlights potential KROSH (DHA/PQP) cardiotoxicity concerns through histological changes, underscoring the need for further research into underlying mechanisms and human health implications. Given DHA/PQP’s wide use, these findings should inform safety evaluations and administration practices.

铁死亡诱导剂RAS合成致死分子3抑制病理性瘢痕成纤维细胞的纤维化

背景:病理性瘢痕主要表现为异常的细胞外基质积累和过度的成纤维细胞增殖,成纤维细胞过度增殖就会产生大量以胶原纤维为主的细胞外基质。因此深入探讨成纤维细胞纤维化在病理性瘢痕形成中的作用,将为揭示病理性瘢痕的机制和生物学治疗提供新思路。目的:探讨铁死亡诱导剂RAS合成致死分子3(RAS-selective lethal small molecule 3,RSL3)对人病理性瘢痕成纤维细胞纤维化的影响。方法:收集10例宁夏医科大学总医院烧伤整形美容科提供的病理性瘢痕组织和同一个体正常皮肤组织,提取人病理性瘢痕成纤维细胞和人正常皮肤成纤维细胞用于后续实验;苏木精-伊红染色观察病理性瘢痕组织和正常皮肤组织的形态;倒置显微镜观察病理性瘢痕成纤维细胞和正常皮肤成纤维细胞的外观形态;免疫荧光实验验证所提取的细胞是否为成纤维细胞;用不同浓度的RSL3(1,3,5,7,9,11,13μmol/L)干预细胞,CCK-8法检测RSL3作用于成纤维细胞的半数抑制浓度(IC_(50));设置对照组(不做处理)和RSL3干预组(用7μmol/L的RSL3干预细胞24 h),qRT-PCR和Western blot检测谷胱甘肽过氧化物酶4、Ⅰ型胶原蛋白、Ⅲ型胶原蛋白和α-平滑肌肌动蛋白的mRNA和蛋白的表达;检测细胞丙二醛浓度;划痕试验检测细胞划痕后24 h剩余划痕面积,并计算剩余划痕面积百分比。结果与结论:①与正常皮肤组相比,病理性瘢痕组的谷胱甘肽过氧化物酶4高表达(mRNA:t=3.252,P<0.01;蛋白:t=5.075,P<0.01);②与正常皮肤成纤维细胞组相比,病理性瘢痕成纤维细胞组的谷胱甘肽过氧化物酶4高表达(mRNA:t=10.32,P<0.01;蛋白:t=26.22,P<0.01);③与对照组相比,RSL3干预组谷胱甘肽过氧化物酶4表达减少(mRNA:t=2.798,P<0.05;蛋白:t=4.643,P<0.01),丙二醛浓度上升(t=2.917,P<0.05),Ⅰ型胶原蛋白(mRNA:t=15.84,P<0.01;蛋白:t=4.610,P<0.01)、Ⅲ型胶原蛋白(mRNA:t=28.86,P<0.01;蛋白:t=7.713,P<0.01)和α-平滑肌肌动蛋白(mRNA:t=2.671,P<0.05;蛋白:t=7.417,P<0.01)的表达减少,迁移能力减弱(t=14.06,P<0.01);④提示RSL3通过抑制谷胱甘肽过氧化物酶4的表达,进而抑制病理性瘢痕成纤维细胞的纤维化和迁移能力。

骨骼肌单器官血管炎下肢肌肉磁共振成像特征及分型

目的:探究骨骼肌单器官血管炎(SoSMV)患者下肢肌肉磁共振成像(MRI)特征和临床分型。方法:回顾分析中日友好医院炎性肌病研究室/肌炎重点实验室2017年1月—2022年12月肌肉病理确诊的SoSMV患者的肌肉病理以及下肢肌肉MRI,包括治疗前的T1加权像(T1WI)、T2加权脂肪抑制像(T2WI-fs)和钆增强T1加权脂肪抑制像(Gd-T1WI-fs)。结果:共纳入SoSMV患者13例,其中男7例,女6例,中位发病年龄49岁。根据患者下肢肌肉MRI和肌肉病理特征,将患者分为2个亚型。1型SoSMV(n=6)患者下肢肌肉MRI均表现为受累肌肉明显肿胀,T1WI上表现为低信号伴有肌间脂肪信号数量减少,T2WI-fs上表现为弥漫分布的高信号,Gd-T1WI-fs上2例表现为比较均匀的强化;肌肉病理4例表现为坏死性血管炎,5例出现管腔闭塞,5例肌纤维变性、坏死伴炎细胞浸润和纤维脂肪组织增生。2型SoSMV(n=7),肌肉MRI均表现为受累肌肉无明显肿胀,T2WI上肌肉内可见边界不清的分枝状条纹高信号,即“鱼骨征”,相应部位在T1WI上的信号无异常,在Gd-T1WI-fs上3例表现为轻度强化;肌肉病理7例均表现为非坏死性血管炎伴血管周围炎。2型SoSMV患者的持续缓解率高于1型。结论:SoSMV患者下肢肌肉MRI和肌肉病理具有特征性表现,在SoSMV的诊断、分型和预后判断上为潜力指标。

表观弥散系数定量评估兔肢体高压电烧伤早期肌肉进行性损伤

目的观察表观弥散系数(ADC)定量评价兔肢体高压电烧伤早期肌肉进行性损伤的价值。方法选取20只健康成年兔建立兔肢体高压电烧伤模型,并将其随机分为0.5、24、48及72 h组,每组5只,分别于造模后各相应时间采集右后肢MR弥散加权成像(DWI),并于其后取双侧肢体肌肉行HE染色;对比各组电流入口及出口损伤核心肌肉与正常肌肉的ADC、肌纤维密度(MFD)及肌纤维短径(D)值,分析兔损伤核心肌肉ADC值与MFD及D值的相关性。结果各组内入口及出口损伤核心肌肉与正常肌肉ADC值、入口与出口损伤核心肌肉ADC值差异均有统计学意义(P均<0.05);入口及出口核心损伤肌肉ADC值随造模时间延长而降低(P均<0.05),而正常肌肉ADC值在不同时间点差异无统计学意义(P>0.05)。入口及出口损伤核心肌肉MFD值随造模时间延长而降低(P均<0.05),而正常肌肉MFD值,以及入口、出口损伤核心和正常肌肉D值在造模不同时间点差异均无统计学意义(P均>0.05)。损伤核心肌肉ADC与MFD值正相关(r_(s)=0.846,P<0.05)、与D值呈负相关(r=-0.507,P<0.05)。结论ADC可定量评价兔肢体高压电烧伤早期肌肉进行性损伤。

基于组织及病理变化探讨筋针疗法对慢性骨骼肌损伤的效应机制

目的基于组织及病理变化探讨筋针疗法对慢性骨骼肌损伤的效应机制。方法选取健康雄性SD大鼠12只,采用随机数字表法分为对照组6只,造模成功后不处理;实验组6只,造模成功后给予筋针疗法治疗。比较2组雌激素相关受体α(ERRα)、乙酰化酶3(SIRT3)、锰超氧化物歧化酶(SOD2)蛋白表达,肌酸激酶(CK)、乳酸脱氢酶(LDH)活性。结果实验组ERRα、SIRT3、SOD2蛋白表达均高于对照组(P<0.05);实验组CK、LDH表达均低于对照组(P<0.05)。结论筋针治疗慢性骨骼肌损伤通过调控线粒体生物合成,降低氧化应激反应,有利于慢性骨骼肌的修复。