Erxian decoction potentially prevents postmenopausal osteoporosis by modulating miR-335: a study based on bioinformatics analysis and preliminary clinical case validation

Background:miRNAs are closely related to bone metabolism.Studies have shown that Erxian decoction can improve bone metabolism,possibly achieving this regulatory effect through miRNA targets.Netinfer was used to predict the miRNA targets of Erxian decoction for the treatment of postmenopausal osteoporosis,and the results were validated by clinical trials.Methods:In this study,we identified possible targets of Erxian decoction in osteoporosis by means of network pharmacological analysis and bioinformatic prediction.Fifteen cases of postmenopausal osteoporosis with kidney Yin and Yang deficiency(In traditional Chinese medicine,kidney Yin nourishes and moistens the tissues of the internal organs of the body,while kidney Yang promotes and warms the tissues of the internal organs of the body.)were treated with Erxian decoction for four weeks,and serum bone metabolism indices(P1NP,osteocalcin,andβ-CTX)and miRNA-335-5p expression were measured before and after treatment.Results:The constructed miRNA postmenopausal osteoporosis related gene network of the effective compound of the Erxian decoction has 296 points and 981 edges.The 39 postmenopausal osteoporosis related genes regulated by miRNA-335-5p were enriched in ossification,while the signaling pathways were enriched in rheumatoid arthritis,the Toll signaling pathway,the HIF-1 signaling pathway,and the MAPK signaling pathway.After taking Erxian decoction,the expression of the serum bone formation index(P1NP,osteocalcin)and miRNA-335-5p gene expression levels increased significantly.The alterations in P1NP and osteocalcin were correlated with the changes in miRNA-335-5p.Conclusion:Circulating miRNA-335-5p may serve as an important target of Erxian decoction in the treatment of postmenopausal women.The effect of Erxian decoction on bone formation is significant,but the underlying mechanism requires further investigation.

Creutzfeldt-Jakob disease presenting as Korsakoff syndrome caused by E196A mutation in PRNP gene:A case report

BACKGROUND Prion diseases are a group of degenerative nerve diseases that are caused by infectious prion proteins or gene mutations.In humans,prion diseases result from mutations in the prion protein gene(PRNP).Only a limited number of cases involving a specific PRNP mutation at codon 196(E196A)have been reported.The coexistence of Korsakoff syndrome in patients with Creutzfeldt-Jakob disease(CJD)caused by E196A mutation has not been documented in the existing literature.CASE SUMMARY A 61-year-old Chinese man initially presented with Korsakoff syndrome,followed by rapid-onset dementia,visual hallucinations,akinetic mutism,myoclonus,and hyperthermia.The patient had no significant personal or familial medical history.Magnetic resonance imaging of the brain revealed extensive hyperintense signals in the cortex,while positron emission tomography/computed tomography showed a diffuse reduction in cerebral cortex metabolism.Routine biochemical and microorganism testing of the cerebrospinal fluid(CSF)yielded normal results.Tests for thyroid function,human immunodeficiency virus,syphilis,vitamin B1 and B12 levels,and autoimmune rheumatic disorders were normal.Blood and CSF tests for autoimmune encephalitis and autoantibody-associated paraneoplastic syndrome yielded negative results.A test for 14-3-3 protein in the CSF yielded negative results.Whole-genome sequencing revealed a diseasecausing mutation in PRNP.The patient succumbed to the illness 11 months after the initial symptom onset.CONCLUSION Korsakoff syndrome,typically associated with alcohol intoxication,also manifests in CJD patients.Individuals with CJD along with PRNP E196A mutation may present with Korsakoff syndrome.

17α-hydroxylase/17,20 carbon chain lyase deficiency caused by p.Tyr329fs homozygous mutation:Three case reports

p.Tyr329fs is a cytochrome P450c17 mutation among Chinese individuals.However,data on 17-α-hydroxylase deficiency caused by cytochrome P450c17 p.Tyr329fs homozygous mutation are lacking.This paper is a case report of three patients homozygous for p.Tyr329fs who were diagnosed with 17-α-hydroxylase deficiency between 2005 and 2019.CASE SUMMARY Case 1 presented with hypertension,hypokalemia,sexual infantilism and delayed bone age.The patient had a 46,XY karyotype,was homozygous for p.Tyr329fs and was recently treated with dexamethasone 0.375 mg qn.Case 2 presented with hypokalemia,sexual infantilism,osteoporosis and delayed bone age.The patient had a 46,XY karyotype,was homozygous for p.Tyr329fs and was treated with dexamethasone 0.75 mg qn at the last follow-up.Serum potassium and blood pressure could be maintained within normal range for cases 1 and 2.Case 3 presented with amenorrhea,sexual infantilism,osteopenia and delayed bone age.The patient had a 46,XX karyotype,was homozygous for p.Tyr329fs and was treated with dexamethasone 0.75 mg qn and progynova 1 mg qd.Outpatient follow-up revealed an adrenocorticotropic hormone(8 AM)of<5.00 pg/mL.CONCLUSION The homozygous p.Tyr329fs mutation usually manifests as a combined deficiency,and definitive diagnosis depends primarily on genetic testing.

基于Omega方法的ASME CC2605-4&3蠕变计算对比分析

规范案例ASME CC2605-4于2021年7月实施。与CC2605-3相比,新标准的蠕变参数(Omega参数和应变率参数)有较大变化。通过解析计算,探讨了温度和等效应力对新旧规范蠕变参数的影响;结合算例,利用Ansys Workbench验算了新旧规范的蠕变损伤,并与解析计算形成对比。结果表明,有限元与解析计算结果吻合,新旧规范中蠕变参数随温度、等效应力变化的规律是一致的,且新规范的Omega参数随等效应力单调递增变化的起点更低,可进一步降低蠕变子程序开发时的最低应力限制,更贴合实际;当温度恒定400℃,初始应力高于220 MPa时,CC2605-4的蠕变损伤总是高于CC2605-3的数值。

ASME规范案例2605-2的修订解读及算法实现

ASME规范案例2605于2008年10月出版,其目的是为了满足炼化行业中加氢工艺设备蠕变疲劳寿命设计的需要。2010年1月第1次修订,即ASME规范案例2605-1,目前,最新的版本为2015年6月第2次修订的ASME规范案例2605-2,这一版修订中有如下重要修改:温度上限由454℃(850℉)提高到了482℃(900℉);对上版有误的公式和表格数据及温度单位进行了修正;在蠕变棘轮的校核中引入了弹性分析来代替非弹性分析。将对ASME规范案例2605-2的分析步骤、修订要点和算法实现进行解读和探讨,为国内工程应用和规范修订提供参考。

基于EERD的建模CASE工具的体系结构

基于EERD建模的CASE工具主要是针对数据库应用的开发 ,它可以提高数据库应用的开发效率和正确性。主要对CASE工具应具有的基本体系结构及其功能进行了介绍 ,并对其实现技术等问题进行了详细阐述 ,最后综合多种流行的CASE工具 ,给出了CASE工具本身的数据字典的概念数据模型的整体结构 。

空间数据库数据模型的研究与应用——以巴尔喀什-准噶尔地区为例

空间数据库是GIS的核心与关键,而数据模型是空间数据库的前提与基础。因此,设计并创建空间数据库数据模型显得尤为重要。研究了基于空间数据库的概念模型,逻辑模型,定义了构成数字地质图各要素间的逻辑关系,重点将ArcGIS的CASE工具与Visio 2003相结合详细构建跨越国境巴尔喀什-准噶尔地区地质图的物理模型,合理地将空间数据和属性数据有效地融合在一起,完成了面向对象数字地质图数据模型的建立。

考虑蠕变作用的2.25Cr-1Mo-V钢加氢反应器设计方法

以某台2.25Cr-1Mo-V钢加氢反应器为例,从工程角度介绍现行设计方法与ASME Code Case 2605-3在反应器设计中的应用,说明过去留有一定余量的设计是设备安全运行的原因之一。探讨了压力和温度载荷对蠕变参数的影响,其中压力对三轴度系数影响较大,而温度对蠕变松弛影响显著。最后提出在业主非强制要求进行完整分析的前提下,ASME Code Case 2605-3在工程上的简化应用。

Unexpected metastasis of intraductal papillary neoplasm of the bile duct without an invasive component to the brain and lungs:A case report

BACKGROUND Despite an expanding number of studies on intraductal papillary neoplasm of the bile duct(IPNB),distant metastasis remains unexplained especially in cases of carcinoma in situ.In the present study,we report a rare and interesting case of IPNB without invasive components that later metastasized to lungs and brain.CASE SUMMARY A 69-year-old male was referred to our hospital due to suspected cholangiocarcinoma.Laboratory tests on admission reported a mild elevation of alkaline phosphatase,γ-glutamyl transpeptidase,and total bilirubin in serum.Endoscopic retrograde cholangiography revealed a filling defect in the common bile duct(CBD)extending to the left hepatic duct.Peroral cholangioscopy delineated a tumor in the CBD that had a papillary pattern.Multidetector computed tomography and magnetic resonance cholangiopancreatography detected partial blockage ot interlude in the CBD leading to cholestasis without evidence of metastasis.Therefore,a diagnosis of IPNB cT1N0M0 was established.Left hepatectomy with bile duct reconstruction was performed.Pathological examination confirmed an intraepithelial neoplasia pattern without an invasive component and an R0 resection achievement.The patient was monitored carefully by regular examinations.However,at 32 mo after the operation,a 26 mm tumor in the lungs and a 12 mm lesion in the brain were detected following a suspicious elevated CA 19-9 level.Video-assisted thoracoscopic surgery of left upper lobectomy and stereotactic radiotherapy are indicated.In addition to histopathological results,a genomic profiling analysis using whole exome sequencing subsequently confirmed lung metastasis originating from bile duct cancer.CONCLUSION This case highlights the important role of genomic profiling analysis using whole exome sequencing in identifying the origin of metastasis in patients with IPNB.

Giant simple hepatic cyst with multiple elevated serum tumormarkers: A case report

BACKGROUND Simple hepatic cysts are relatively common in adults, and mostly appear asasymptomatic incidental radiologic findings. Occasionally, a large cyst will causesymptoms. Elevations in the serum biomarkers protein induced by vitamin Kabsence (PIVKA)-II, cancer antigen (CA) 12-5, and CA19-9 are often associatedwith malignant tumors in the liver or bile ducts. This is the first report to describea case of hepatic cyst with elevated levels of PIVKA-II and CA12-5.CASE SUMMARY An 84-year-old Chinese woman was admitted with gradual abdominal distension.Her symptoms started 1 year ago, and she had poor appetite and a weight loss of5 kg within the past 2 wk. She denied any symptoms associated with abdominalpain, fever and chills, nausea and vomiting, etc. The abdomen was enlarged, morein the right upper quadrant, without tenderness. Laboratory examination showedsignificantly increased serum levels of PIVKA-II, CA12-5, and CA19-9. Acomputed tomography scan revealed multiple round cysts in the liver with clearboundaries. The largest cyst was 20.1 cm × 12.2 cm × 19.6 cm in size, located in theright lobe of the liver with mild dilatation of the intrahepatic bile duct, but therewas no contrast enhancement. Percutaneous drainage on the largest hepatic cystand polycinnamol sclerosing agent injection into the cyst cavity were performed.After treatment, the patient’s symptoms relieved and the elevated serum tumormakers reduced to the normal levels dramatically.CONCLUSION The present report identifies an unusual case of a giant hepatic cyst with markedelevation of serum tumor marker levels of PIVKA-II, CA12-5, and CA19-9. Aftertreatment, these three serum markers dramatically decreased to normal levels.The mechanisms for the elevation of these tumor markers may be as follows: (1) Agiant hepatic cyst compresses the liver, causing injury to the hepatocytes, whichmay lead to secretion of a large amount of PIVKA-II;and (2) Some tumorassociatedantigens, such as carcinoembryonic antigen, CA19-9, CA12-5, andCA15-3, are expressed on inflammatory cells.

Effect of abrasive blasting on the internal surface of L80-13Cr tubing and casing

In this study,two sandblasting textures,namely,quartz and high-chromium(Cr)stainless steel sands,were used for sandblasting of the internal surface L80-13Cr tubing and casing.The contrastive analysis on the properties of the pipes before and after abrasive blasting was conducted,including the metallographic phase detection of the internal surface,simulating accelerated corrosion tests,and residual stress tests for the pipe after abrasive blasting.Based on the analysis results,it is explained why the API standard requires that no scale appears on the internal surface of L80-13Cr and iron contamination when internal sandblasting media are applied.The results show that abrasive blasting can effectively remove scales on the internal surface of L80-13Cr tubing and casing and improve corrosion resistance.The abrasive blasting process does not produce obvious residual stress on the internal surface of the tubing and casing.Also,the de-rusting effect of stainless sand is better than that of quartz sand,and the former does not produce iron contamination.

ASME Code Case 2843技术基础分析

ASME Code Case 2843(以下简称为“案例”)提供了针对高温工况和交变载荷下承受蠕变和疲劳损伤交互作用的受压元件进行评定的方法。在应用该案例方法的过程中,如不能理解这些方法的技术基础,则可能造成评定结果的不安全。案例中所提出的蠕变-疲劳损伤评定方法主要利用线弹性分析得到的结果对弹塑性材料构成的受压元件进行评定。整套方法包括载荷控制极限的判定、应变控制极限的判定以及蠕变-疲劳损伤极限的判定三个步骤,每个步骤中采用的方法均依据多年来有关研究人员发表文献中的理论和实验结果。拟对案例中所采用方法的技术基础进行分析,为工程中应用案例的方法对承受高温和交变载荷作用的压力容器进行设计提供一定的参考。

Iatrogenic giant pseudomeningocele of the cervical spine:A case report

BACKGROUND Only a few cases of giant pseudomeningoceles have been reported in the literature.Herein,we report a giant pseudomeningocele of the cervical spine that was found after cervical laminectomy for an epidural hematoma following epidural blockade.CASE SUMMARY A 47-year-old man presented with recurrent neck pain and posterior neck swelling after spinal surgery.Magnetic resonance imaging of the cervical spine revealed fluid collection(5.6 cm×6.6 cm×11.2 cm)at the C3-6 level;this proved to be a pseudomeningocele.Symptoms related to the pseudomeningocele resolved following dural repair and fat graft transplantation.CONCLUSION Although rare,pseudomeningocele is a possibility in patients with recurrent back pain,radicular pain,or a persistent headache following spinal surgery.Continuous attention should be paid throughout the spinal procedure,whether conservative or non-conservative.

基于情境知晓的知识管理系统设计与应用

从本体出发,设计了基于情境知晓的知识管理系统的模型框架,将用户本体、文档本体、任务本体、领域知识本体、应用情景本体作为一个明晰的概念在模型中加以体现,在此基础上架构了基于情境知晓的知识管理系统,并将其应用到实际企业中。

Analysis of stable components for extended-range (10-30 days) weather forecast:A case study of continuous overcast-rainy process in early 2009 over the mid-lower reaches of the Yangtze River

A continuous overcast-rainy weather(CORW) process occurred over the mid-lower reaches of the Yangtze River(MLRYR) in China from February 14 to March 9 in 2009,with a large stretch and long duration that was rarely seen in historical records.Using the empirical orthogonal function(EOF),we analyzed the geopotential height anomaly field of the NCEP-DOE Reanalysis II in the same period,and defined the stable components of extended-range(10-30 days) weather forecast(ERWF).Furthermore,we defined anomalous and climatic stable components based on the variation characteristics of the variance contribution ratio of EOF components.The climatic stable components were able to explain the impact of climatically averaged information on the ERWF,and the anomalous stable components revealed the abnormal characteristics of the continuous overcast-rainy days.Our results show that the stable components,especially the anomalous stable components,can maintain the stability for a longer time(more than 10 days) and manifest as monthly scale low-frequency variation and ultra-long-wave activities.They also behave as ultra-long waves of planetary scale with a stable and vertically coherent structure,reflect the variation of general circulation in mid-high latitudes,display the cycle of the zonal circulation and the movement and adjustment of the ultra-long waves,and are closely linked to the surface CORW process.

The first laboratory-confirmed neonatal Mpox infection in Sri Lanka

In 2022-2023,a global outbreak of Mpox was reported especially in nonendemic countries.We report the first laboratory-confirmed neonatal case of Mpox infection complicated by bronchopneumonia in Sri Lanka.

STUDY ON CRYSTAL STRUCTURE OF DESHEPTAPEPTIDE(B24-30) INSULIN——FINDING OF A NEW CRYSTAL FORM

The structure-function relationship of insulin to be understood at three-dimensional level has already been a challenging problem since the elucidation of fine crystal structures of insulin. Of course, it is a better experimental approach to such a problem that certain insulin-receptor complexes were prepared and their structures were

CASE革命之 开篇 自动驾驶,驶向何方?

未来汽车“四化”之间的关系可以高度概括为电动化是基础,网联化是条件,智能化是关键,共享化是趋势。“未来汽车‘四化’之间的关系可以高度概括为‘电动化是基础,网联化是条件,智能化是关键,共享化是趋势。”一汽-大众奥迪在回复《商学院》记者采访时表示。如今,汽车产业正经历前所未有的变革,人工智能、新能源、共享等新技术、新趋势,正推动汽车产业进入新时代。“未来汽车的‘四化’是必然趋势,完全符合时代发展和社会进步,