To test the efficacy of two calcium phosphate pastes compared to that of fluoride toothpaste on remineralizing artificial caries in situ, this study had a double-blind crossover in situ design, involving three experim...To test the efficacy of two calcium phosphate pastes compared to that of fluoride toothpaste on remineralizing artificial caries in situ, this study had a double-blind crossover in situ design, involving three experimental phases of 14 days each, with an 8-day washout period between phases. Nine healthy subjects participated in the study. The subjects wore removable palatal appliances mounted with six human enamel slabs with artificial caries lesions, and in each of the experimental phases, used one of the following methods two times/day： group A, brushing with 1.0 g of Colgate Regular Flavor, followed by applying 0.25 g of Tooth Mousse Plus; group B, brushing with 0.25 g of Clinpro Tooth Crbme; and group C, brushing with 1.0 g of Colgate Regular Flavor. After 14 days, the enamel slabs （54 slabs/ group） were embedded in resin, sectioned and examined with a polarized-light microscope, and the lesion areas were quantified using Image-Pro Plus. All experimental groups showed a significant reduction in lesion area compared to the initial lesion area （paired t-test, P〈O.O01）. The mean reduction in lesion area of Groups A, B and C were （0.029__.0.010）, （0.030_＋0.009） and （0.027_＋0.009） mm2, respectively. There were no statistical differences between groups （KruskaI-Wallis test, P〉0.05）. All three groups remineralized the enamel slab lesions, indicating model sensitivity to fluoride. Given the differences in usage amounts and treated regimens, Clinpro Tooth Crbme provided similar benefits to the fluoride toothpaste; however, no additional benefit of Tooth Mousse Plus was observed when used in conjunction with the fluoride toothpaste.展开更多
The aim of this study is to evaluate the influence of Tooth Mousse （TM） application, smear layer removal, and storage time on resin-dentin microtensile bond strength （pTBS）. Dentin specimens were divided into two ...The aim of this study is to evaluate the influence of Tooth Mousse （TM） application, smear layer removal, and storage time on resin-dentin microtensile bond strength （pTBS）. Dentin specimens were divided into two groups： （1） smear layer covered; （2） smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive （Clearfil SE Bond （CSE）; Kuraray Medical, Tokyo, Japan）, an all-in-one adhesive （G-Bond （GB）; GC Corp, Tokyo, Japan）, and a total-etch adhesive （Adper Single Bond 2 （SB）; 3M ESPE, St. Paul, MN, USA）, the specimens were stored for 3 d or 6 months in deionized water at 37 ℃, and pTBS was tested and analyzed. With the exception of SB （no TM application） and GB, the pTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage （P≤0.001）. Bond strength of GB decreased significantly when using EDTA （3 d storage, P〈0.05）. TM application only increased the pTBS of GB （no EDTA） and SB （with EDTA） after 3 d （P≤0.02）. Comparing the adhesives after 3 d of storage, CSE exhibited the greatest pTBS values followed by GB and SB （P≤0.02）. The factors of adhesive, EDTA, and TM did not show any significant impact on pTBS when specimens were stored for 6 months （P〉0.05）. The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on pTBS of dentin bonds after a period of 6 months.展开更多
文摘To test the efficacy of two calcium phosphate pastes compared to that of fluoride toothpaste on remineralizing artificial caries in situ, this study had a double-blind crossover in situ design, involving three experimental phases of 14 days each, with an 8-day washout period between phases. Nine healthy subjects participated in the study. The subjects wore removable palatal appliances mounted with six human enamel slabs with artificial caries lesions, and in each of the experimental phases, used one of the following methods two times/day： group A, brushing with 1.0 g of Colgate Regular Flavor, followed by applying 0.25 g of Tooth Mousse Plus; group B, brushing with 0.25 g of Clinpro Tooth Crbme; and group C, brushing with 1.0 g of Colgate Regular Flavor. After 14 days, the enamel slabs （54 slabs/ group） were embedded in resin, sectioned and examined with a polarized-light microscope, and the lesion areas were quantified using Image-Pro Plus. All experimental groups showed a significant reduction in lesion area compared to the initial lesion area （paired t-test, P〈O.O01）. The mean reduction in lesion area of Groups A, B and C were （0.029__.0.010）, （0.030_＋0.009） and （0.027_＋0.009） mm2, respectively. There were no statistical differences between groups （KruskaI-Wallis test, P〉0.05）. All three groups remineralized the enamel slab lesions, indicating model sensitivity to fluoride. Given the differences in usage amounts and treated regimens, Clinpro Tooth Crbme provided similar benefits to the fluoride toothpaste; however, no additional benefit of Tooth Mousse Plus was observed when used in conjunction with the fluoride toothpaste.
基金Project supported by the National Natural Science Foundation of China(Nos.81271955 and 30973350)the Zhejiang Provincial Natural Science Foundation of China(No.Y2080338)
文摘The aim of this study is to evaluate the influence of Tooth Mousse （TM） application, smear layer removal, and storage time on resin-dentin microtensile bond strength （pTBS）. Dentin specimens were divided into two groups： （1） smear layer covered; （2） smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive （Clearfil SE Bond （CSE）; Kuraray Medical, Tokyo, Japan）, an all-in-one adhesive （G-Bond （GB）; GC Corp, Tokyo, Japan）, and a total-etch adhesive （Adper Single Bond 2 （SB）; 3M ESPE, St. Paul, MN, USA）, the specimens were stored for 3 d or 6 months in deionized water at 37 ℃, and pTBS was tested and analyzed. With the exception of SB （no TM application） and GB, the pTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage （P≤0.001）. Bond strength of GB decreased significantly when using EDTA （3 d storage, P〈0.05）. TM application only increased the pTBS of GB （no EDTA） and SB （with EDTA） after 3 d （P≤0.02）. Comparing the adhesives after 3 d of storage, CSE exhibited the greatest pTBS values followed by GB and SB （P≤0.02）. The factors of adhesive, EDTA, and TM did not show any significant impact on pTBS when specimens were stored for 6 months （P〉0.05）. The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on pTBS of dentin bonds after a period of 6 months.
