Objective To study the expression of a inhibito r gene,survivin , in pituitary adenomas and its relationship with the expression of caspase-3gen e.Method Using streptavidin-biotin peroxid ase(SP),the expression of sur...Objective To study the expression of a inhibito r gene,survivin , in pituitary adenomas and its relationship with the expression of caspase-3gen e.Method Using streptavidin-biotin peroxid ase(SP),the expression of surviv ing,caspa se-3protein was examined in 8normal pituitary tissue and 38pitu itary adenomas.Result Survivin was expressed in 23of 38cases of pituitary a denomas,in contrast,normal pituitary tissue did not express surviving.Positiv e expression rate of PRL group,GHgroup,Mixed group in pituitary adenomas was 12/17,7/13,4/8,respective-ly.Overexpression of surviving was no t related with the tumor type(P >0.05).Meanwhile,surviving positive rat e in caspase-3positive and negative groups was 5/14,79%.The result s how ed it was strongly associated with ca spase-3expression(P <0.05).C onclusions Apoptosis suppression by surviving play an important role in a popto sis of pituitary adenomas.Sur vivin could be a new diagnostic /therapeutic tar get in pituitary adenoma s.展开更多
目的探讨三黄连合剂通过NOD样受体蛋白3(NOD-like receptor protein domain associated protein 3,NLRP3)/凋亡相关颗粒样蛋白(apoptosis-associated speck-like protein containing a CARD,ASC)/半胱氨酸天冬氨酸蛋白酶1(cysteine aspa...目的探讨三黄连合剂通过NOD样受体蛋白3(NOD-like receptor protein domain associated protein 3,NLRP3)/凋亡相关颗粒样蛋白(apoptosis-associated speck-like protein containing a CARD,ASC)/半胱氨酸天冬氨酸蛋白酶1(cysteine aspartic acid specific protease-1,Caspase-1)通路对哮喘患儿气道炎症的作用及机制。方法选取2021年1月—2023年10月就诊的90例哮喘患儿,采用数字表法随机分为观察组与对照组,各45例。观察2组哮喘患儿不良反应、1 s用力呼气容积(forced expiratory volume in the first second,FEV1%)、最大呼气流量(peak expiratory flow,PEF)、用力肺活量(forced vital capacity,FVC)、白细胞介素-1β(interleukin-1β,IL-1β)及TNF-α(tumor necrosis factor-α,TNF-α)表达水平差异。结果观察组不良反应发生率为6.66%,对照组为11.11%,差异无统计学意义(P>0.05)。观察组总有效率为95.56%,高于对照组的80.00%(P<0.05)。治疗后FEV1%、PEF、FVC、哮喘控制测试表(asthma control test,ACT)评分高于对照组(P<0.05)。治疗后观察组血清IL-1β、IL-6、IL-8、IL-17、IL-18水平低于对照组(P<0.05)。治疗后观察组患儿血清NLRP3、ASC、Caspase-1、IL-1β蛋白表达水平低于对照组(P<0.05)。结论三黄连合剂治疗能减轻哮喘患儿气道炎症反应,降低炎症因子水平,改善肺功能,减轻病情,提高疗效,其可能通过NLRP3/ASC/Caspase-1通路发挥作用。展开更多
文摘Objective To study the expression of a inhibito r gene,survivin , in pituitary adenomas and its relationship with the expression of caspase-3gen e.Method Using streptavidin-biotin peroxid ase(SP),the expression of surviv ing,caspa se-3protein was examined in 8normal pituitary tissue and 38pitu itary adenomas.Result Survivin was expressed in 23of 38cases of pituitary a denomas,in contrast,normal pituitary tissue did not express surviving.Positiv e expression rate of PRL group,GHgroup,Mixed group in pituitary adenomas was 12/17,7/13,4/8,respective-ly.Overexpression of surviving was no t related with the tumor type(P >0.05).Meanwhile,surviving positive rat e in caspase-3positive and negative groups was 5/14,79%.The result s how ed it was strongly associated with ca spase-3expression(P <0.05).C onclusions Apoptosis suppression by surviving play an important role in a popto sis of pituitary adenomas.Sur vivin could be a new diagnostic /therapeutic tar get in pituitary adenoma s.
文摘目的探讨三黄连合剂通过NOD样受体蛋白3(NOD-like receptor protein domain associated protein 3,NLRP3)/凋亡相关颗粒样蛋白(apoptosis-associated speck-like protein containing a CARD,ASC)/半胱氨酸天冬氨酸蛋白酶1(cysteine aspartic acid specific protease-1,Caspase-1)通路对哮喘患儿气道炎症的作用及机制。方法选取2021年1月—2023年10月就诊的90例哮喘患儿,采用数字表法随机分为观察组与对照组,各45例。观察2组哮喘患儿不良反应、1 s用力呼气容积(forced expiratory volume in the first second,FEV1%)、最大呼气流量(peak expiratory flow,PEF)、用力肺活量(forced vital capacity,FVC)、白细胞介素-1β(interleukin-1β,IL-1β)及TNF-α(tumor necrosis factor-α,TNF-α)表达水平差异。结果观察组不良反应发生率为6.66%,对照组为11.11%,差异无统计学意义(P>0.05)。观察组总有效率为95.56%,高于对照组的80.00%(P<0.05)。治疗后FEV1%、PEF、FVC、哮喘控制测试表(asthma control test,ACT)评分高于对照组(P<0.05)。治疗后观察组血清IL-1β、IL-6、IL-8、IL-17、IL-18水平低于对照组(P<0.05)。治疗后观察组患儿血清NLRP3、ASC、Caspase-1、IL-1β蛋白表达水平低于对照组(P<0.05)。结论三黄连合剂治疗能减轻哮喘患儿气道炎症反应,降低炎症因子水平,改善肺功能,减轻病情,提高疗效,其可能通过NLRP3/ASC/Caspase-1通路发挥作用。
