[Objective] This study aimed to investigate the effects of drought stress on osmotic regulation substances of Catalpa bungei clones. [Method] Potting experimental was carried out with plastic film sealing method, to d...[Objective] This study aimed to investigate the effects of drought stress on osmotic regulation substances of Catalpa bungei clones. [Method] Potting experimental was carried out with plastic film sealing method, to differentiate the degrees of drought stress based on changes in soil water content and leaf water potential and investigate variations in contents of osmotic regulation substances（free proline,soluble sugar and soluble protein） in leaves of five Catalpa bungei clones under different degrees of drought stresses. [Result] According to changes in soil water content and leaf water potential, the whole process of drought stress was divided into normal level（CK, with soil water content of 97.49% and leaf water potential of-0.54MPa）, light drought（LD with soil water content of 59.96% and leaf water potential of-1.28 MPa）, mediate drought（MD with soil water content of 34.19% and leaf water potential of-2.32 MPa） and severe drought（SD with soil water content of 14.52%and leaf water potential of-2.99 MPa）. The soil water content and leaf water potential of five Catalpa bungei clones reached the highest correlation in exponential fitting, with an average R2of 0.989 3（P0.001）. The free proline content in leaves of five Catalpa bungei clones increased rapidly with the increasing degree of drought stress（P 0.001）. To be specific, free proline contents of Catalpa bungei clones015-1 and 7080 were 34.39 and 33.41 times of the normal level under severe drought conditions, which reached an extremely significant level（P0.001）; the free proline content of Catalpa bungei clone 1-3 rapidly increased to（855.46±227.52） μg/g Fw under light drought conditions. The soluble protein content in leaves showed different variation trends. To be specific, the soluble protein content of Catalpa bungei clone 7080 was the lowest at various drought stages and reached（1.644 ±0.137）mg/g Fw under normal conditions; the soluble protein content of Catalpa bungei clone 1-3 was relatively high under normal conditions and was reduced rapidly under light drought conditions, showing different response patterns. [Conclusion] Osmotic regulation substances in leaves of five Catalpa bungei clones all have certain response to drought stress. Free proline is the most important osmotic regulation substance, followed by soluble protein, while soluble sugar makes no significant contribution. According to changes in content of osmotic regulation substances, Catalpa bungei clone 7080 has relatively high capacity and good drought resistance, while Catalpa bungei clone 1-3 has the rapidest response to drought stress.展开更多
[Objective] This study aimed to provide scientific basis for the early selec-tion and breeding of excellent Catalpa bungei clones. [Method] The Li-6400 portable photosynthesis system was used to analyze the photosynth...[Objective] This study aimed to provide scientific basis for the early selec-tion and breeding of excellent Catalpa bungei clones. [Method] The Li-6400 portable photosynthesis system was used to analyze the photosynthetic characteristics of five Catalpa bungei clones （1-3, 1-4, 004-1, 015-1 and 7080）. [Result] Al five Catalpa bungei clones had similar change trends of light response curves, but their maxi-mum net photosynthetic rates （Pmax） were in the order of 1-3＆gt;7080＆gt;015-1＆gt;004-1＆gt;1-4, showing that clone 1-3 and clone 7080 had strong light environmental adapt-ability with the light saturation points （LSP） of 1 147.97 μmol/m2＆#183;s and 1 196.31μmol/m2＆#183;s, respectively, while clone 1-4 had the narrowest range of adaptation to light. There were differences in the total photosynthetic amount per area among the five clones that clone 1-4 was the best of 433 974.24 μmol/m2, fol owed by clone 1-3 of 401 290.20 μmol/m2. The transpiration rates （Tr） and stomatal conductances （Cond） of the five clones presented the same change trend. And the diurnal varia-tion curves of net photosynthetic rate （Pn） of clone 1-3, 1-4 and 015-1 showed two typical peaks with obvious midday depression, while clone 004-1 and 7080 didn’t. [Concluson] Considering a variety of photosynthetic characteristics of indica-tors, photosynthetic capacity of clone 1-3 was the best among the five Catalpa bungei clones.展开更多
Lysine acetylation,a reversible and dynamic post-translational modification,plays pivotal roles in regulating many biological processes in plants.However,the information of lysine acetylation during flower development...Lysine acetylation,a reversible and dynamic post-translational modification,plays pivotal roles in regulating many biological processes in plants.However,the information of lysine acetylation during flower development of woody angiosperm remains unclear.Herein,we identified a total of 667 lysine acetylation sites in 467 proteins in flower buds of Catalpa Bungei.The acetylated proteins were mainly involved in the pathways of carbon metabolism,glycometabolism and oxidative phosphorylation.Using functional enrichment analysis,61 and 13 acetylated proteins were involved in the glycometabolism and oxidative phosphorylation pathways,respectively.This suggests that lysine acetylated proteins play critical roles in the energy metabolism pathways.Accordingly,the protein–protein interactions were also highly enriched in the carbon metabolic pathway.Integrative analysis of lysine acetylome and transcriptome showed that transcript level changes of seven key lysine-acetylated proteins/genes involved in energy metabolism were significant different during floral transition.Importantly,compared with the normal flowering variety,the expression levels of phosphoribulokinase,phosphoenolpyruvate carboxykinase,ATP synthase and pyruvate dehydrogenase complex genes were significantly up-regulated at the stages of vegetative and transition buds during early flowering of C.bungei.These data represent the first lysine acetylome in the C.bungei flowers,and can serve as an essential basis for exploring the function of lysine acetylated proteins in the flower bud development of plants.展开更多
为探讨不同埋干深度对嫁接楸树根系生长特性的影响,选用无性系楸树品种中的中豫楸1号作为试验研究对象,共设置8,16,24,32,40 cm 5个埋干深度小组,对各个埋干深度小组楸树苗的苗高、直径、根系数量、根系长度、根系生物量数据进行测定。...为探讨不同埋干深度对嫁接楸树根系生长特性的影响,选用无性系楸树品种中的中豫楸1号作为试验研究对象,共设置8,16,24,32,40 cm 5个埋干深度小组,对各个埋干深度小组楸树苗的苗高、直径、根系数量、根系长度、根系生物量数据进行测定。结果发现,不同埋干深度处理小组间的粗根、中根和细根的数量和长度之间差异不显著(P<0.05),但是随着埋干深度的加深,楸树苗粗根的生物量呈递减趋势,以埋干深度为24 cm组的生物量最高;不同埋干处理小组的粗根、中根、细根的生物量主要集中在0~40 cm的嫁接根下根桩范围;随着埋干深度的不断加深,深层土壤中的生物量则越少。展开更多
The article investigated the changes of nutrients(total sugar,total nitrogen,total sugar/total N)and oxidases(IAAO,POD,PPO)of Catalpa bungei cl.’Yu-1’ in the cutting rooting process.The results showed that:C.bungei ...The article investigated the changes of nutrients(total sugar,total nitrogen,total sugar/total N)and oxidases(IAAO,POD,PPO)of Catalpa bungei cl.’Yu-1’ in the cutting rooting process.The results showed that:C.bungei cl.’Yu-1’ cutting rooting initiated from the lenticel.The soluble protein in the forced-sprouting cuttings decreased dramatically in the early stage,and then the decrease tended to slow.The total sugar content slowly increased throughout.The total nitrogen had a little change.The total sugar/total nitrogen ratio had same trend with total sugar.The IAAO,PPO activity of the forced-sprouting cuttings showed up-down-up-down pattern in the rooting process,The POD activity rose in undulation pattern.The role of three oxidases was both independent with each other,and related to one another in the process of rooting by which affected rooting in integration.展开更多
基金Supported by National Scientific and Technological Project in Rural Areas in the "Twelfth Five Year" Plan(2012BAD21B03,2012BAD21B0304)~~
文摘[Objective] This study aimed to investigate the effects of drought stress on osmotic regulation substances of Catalpa bungei clones. [Method] Potting experimental was carried out with plastic film sealing method, to differentiate the degrees of drought stress based on changes in soil water content and leaf water potential and investigate variations in contents of osmotic regulation substances（free proline,soluble sugar and soluble protein） in leaves of five Catalpa bungei clones under different degrees of drought stresses. [Result] According to changes in soil water content and leaf water potential, the whole process of drought stress was divided into normal level（CK, with soil water content of 97.49% and leaf water potential of-0.54MPa）, light drought（LD with soil water content of 59.96% and leaf water potential of-1.28 MPa）, mediate drought（MD with soil water content of 34.19% and leaf water potential of-2.32 MPa） and severe drought（SD with soil water content of 14.52%and leaf water potential of-2.99 MPa）. The soil water content and leaf water potential of five Catalpa bungei clones reached the highest correlation in exponential fitting, with an average R2of 0.989 3（P0.001）. The free proline content in leaves of five Catalpa bungei clones increased rapidly with the increasing degree of drought stress（P 0.001）. To be specific, free proline contents of Catalpa bungei clones015-1 and 7080 were 34.39 and 33.41 times of the normal level under severe drought conditions, which reached an extremely significant level（P0.001）; the free proline content of Catalpa bungei clone 1-3 rapidly increased to（855.46±227.52） μg/g Fw under light drought conditions. The soluble protein content in leaves showed different variation trends. To be specific, the soluble protein content of Catalpa bungei clone 7080 was the lowest at various drought stages and reached（1.644 ±0.137）mg/g Fw under normal conditions; the soluble protein content of Catalpa bungei clone 1-3 was relatively high under normal conditions and was reduced rapidly under light drought conditions, showing different response patterns. [Conclusion] Osmotic regulation substances in leaves of five Catalpa bungei clones all have certain response to drought stress. Free proline is the most important osmotic regulation substance, followed by soluble protein, while soluble sugar makes no significant contribution. According to changes in content of osmotic regulation substances, Catalpa bungei clone 7080 has relatively high capacity and good drought resistance, while Catalpa bungei clone 1-3 has the rapidest response to drought stress.
基金Supported by the National Key Technology R&D Program(2012BAD21B03)Specialized Research Fund for The Doctoral Program of Higher Education(20124404120008)~~
文摘[Objective] This study aimed to provide scientific basis for the early selec-tion and breeding of excellent Catalpa bungei clones. [Method] The Li-6400 portable photosynthesis system was used to analyze the photosynthetic characteristics of five Catalpa bungei clones （1-3, 1-4, 004-1, 015-1 and 7080）. [Result] Al five Catalpa bungei clones had similar change trends of light response curves, but their maxi-mum net photosynthetic rates （Pmax） were in the order of 1-3＆gt;7080＆gt;015-1＆gt;004-1＆gt;1-4, showing that clone 1-3 and clone 7080 had strong light environmental adapt-ability with the light saturation points （LSP） of 1 147.97 μmol/m2＆#183;s and 1 196.31μmol/m2＆#183;s, respectively, while clone 1-4 had the narrowest range of adaptation to light. There were differences in the total photosynthetic amount per area among the five clones that clone 1-4 was the best of 433 974.24 μmol/m2, fol owed by clone 1-3 of 401 290.20 μmol/m2. The transpiration rates （Tr） and stomatal conductances （Cond） of the five clones presented the same change trend. And the diurnal varia-tion curves of net photosynthetic rate （Pn） of clone 1-3, 1-4 and 015-1 showed two typical peaks with obvious midday depression, while clone 004-1 and 7080 didn’t. [Concluson] Considering a variety of photosynthetic characteristics of indica-tors, photosynthetic capacity of clone 1-3 was the best among the five Catalpa bungei clones.
基金This work was financially supported by the Open Fund of State Key Laboratory of Tree Genetics and Breeding(Chinese Academy of Forestry)(No.TGB2019005)Fundamental Research Funds for the Central Universities(XDJK2020B058).The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
文摘Lysine acetylation,a reversible and dynamic post-translational modification,plays pivotal roles in regulating many biological processes in plants.However,the information of lysine acetylation during flower development of woody angiosperm remains unclear.Herein,we identified a total of 667 lysine acetylation sites in 467 proteins in flower buds of Catalpa Bungei.The acetylated proteins were mainly involved in the pathways of carbon metabolism,glycometabolism and oxidative phosphorylation.Using functional enrichment analysis,61 and 13 acetylated proteins were involved in the glycometabolism and oxidative phosphorylation pathways,respectively.This suggests that lysine acetylated proteins play critical roles in the energy metabolism pathways.Accordingly,the protein–protein interactions were also highly enriched in the carbon metabolic pathway.Integrative analysis of lysine acetylome and transcriptome showed that transcript level changes of seven key lysine-acetylated proteins/genes involved in energy metabolism were significant different during floral transition.Importantly,compared with the normal flowering variety,the expression levels of phosphoribulokinase,phosphoenolpyruvate carboxykinase,ATP synthase and pyruvate dehydrogenase complex genes were significantly up-regulated at the stages of vegetative and transition buds during early flowering of C.bungei.These data represent the first lysine acetylome in the C.bungei flowers,and can serve as an essential basis for exploring the function of lysine acetylated proteins in the flower bud development of plants.
文摘为探讨不同埋干深度对嫁接楸树根系生长特性的影响,选用无性系楸树品种中的中豫楸1号作为试验研究对象,共设置8,16,24,32,40 cm 5个埋干深度小组,对各个埋干深度小组楸树苗的苗高、直径、根系数量、根系长度、根系生物量数据进行测定。结果发现,不同埋干深度处理小组间的粗根、中根和细根的数量和长度之间差异不显著(P<0.05),但是随着埋干深度的加深,楸树苗粗根的生物量呈递减趋势,以埋干深度为24 cm组的生物量最高;不同埋干处理小组的粗根、中根、细根的生物量主要集中在0~40 cm的嫁接根下根桩范围;随着埋干深度的不断加深,深层土壤中的生物量则越少。
文摘The article investigated the changes of nutrients(total sugar,total nitrogen,total sugar/total N)and oxidases(IAAO,POD,PPO)of Catalpa bungei cl.’Yu-1’ in the cutting rooting process.The results showed that:C.bungei cl.’Yu-1’ cutting rooting initiated from the lenticel.The soluble protein in the forced-sprouting cuttings decreased dramatically in the early stage,and then the decrease tended to slow.The total sugar content slowly increased throughout.The total nitrogen had a little change.The total sugar/total nitrogen ratio had same trend with total sugar.The IAAO,PPO activity of the forced-sprouting cuttings showed up-down-up-down pattern in the rooting process,The POD activity rose in undulation pattern.The role of three oxidases was both independent with each other,and related to one another in the process of rooting by which affected rooting in integration.
