The aim of this paper was to develop a cataplasma matrix that can be applicable to both watersoluble and liposoluble drugs.The gellan gum and konjaku were employed as the scaffold materials of the matrix.With polyacry...The aim of this paper was to develop a cataplasma matrix that can be applicable to both watersoluble and liposoluble drugs.The gellan gum and konjaku were employed as the scaffold materials of the matrix.With polyacrylic acid sodium and oligosaccharides as tacktifier,the formula of the cataplasma matrix was optimized in the orthogonal method as:gellan gum 0.4 g,xanthan gum 0.03 g,konjac glue 0.1 g,glycerin 4 g,Gluco-Adhesive T(GAT)6 g,Gluco-Adhesive E(GAE)6 g,polyacrylic acid sodium 0.22 g,and sorbitol 3 g.The 180°peel strength,the tensile strength and the elongation at break was 3.043 N,0.275 MPa and 91.05%,respectively.Furthermore,the drug-compatibilities of the matrix were investigated with baicalin,berberine and curcumin,which were used as the models of hydrophilic,poor-water-soluble and hydrophobic ingredients.The drug contents could reach 4.12%,2.42%and 3.75%,while the in vitro release rate were measured as,361.79,55.85 and 104.41μg·cm^(-2)·h^(-1)for baicalin,berberine and curcumin,respectively.These results indicated that the obtained matrix had good drug-compatibility and drug-release properties for different ingredients.展开更多
为了研究治伤巴布剂对大鼠早期骨折愈合及对肿瘤坏死因子-α(tumornecrosisfactor-α,TNF-α)表达的影响,将45只SPF级雄性大鼠随机分为空白组、模型组、治伤巴布剂组3个组,每组15只。空白组不造模,其余2组予以闭合造模,造模成功后治伤...为了研究治伤巴布剂对大鼠早期骨折愈合及对肿瘤坏死因子-α(tumornecrosisfactor-α,TNF-α)表达的影响,将45只SPF级雄性大鼠随机分为空白组、模型组、治伤巴布剂组3个组,每组15只。空白组不造模,其余2组予以闭合造模,造模成功后治伤巴布剂组在骨折标记部位予治伤巴布剂局部外敷;模型组则予等剂量赋形剂局部外敷(每天换药一次),干预7 d。造模后分别于1、3、7 d 3个时相点观测大鼠骨折肢体肿胀度、苏木精-伊红染色法(HE)观察骨折断端病理形态变化、酶联免疫吸附试验(ELISA)检测血清中TNF-α水平。结果表明:(1)与空白组对比,模型组及治伤巴布剂组在1、3、7 d 3个时相点大鼠右侧股骨明显肿胀(P<0.01),治伤巴布剂组在1、3、7 d 3个时相点肢体肿胀度较模型组明显减轻(P<0.01)。(2)HE染色结果显示模型组中可见少量软骨细胞和肉芽组织,未见骨小梁形成;治伤巴布剂可见大量软骨细胞和肉芽组织,并可见少量骨小梁形成。(3)ELISA结果显示与空白组相比,模型组及治伤巴布剂组TNF-α水平明显升高(P<0.01);与模型组相比,治伤巴布剂组TNF-α水平明显降低(P<0.01)。治伤巴布剂可以减轻大鼠股骨中段的肿胀度,对骨折的早期愈合具有一定促进作用,且降低TNF-α浓度水平抑制炎症反应。展开更多
基金the National Natural Science Foundation of China for the financial support(Grant No.20606024).
文摘The aim of this paper was to develop a cataplasma matrix that can be applicable to both watersoluble and liposoluble drugs.The gellan gum and konjaku were employed as the scaffold materials of the matrix.With polyacrylic acid sodium and oligosaccharides as tacktifier,the formula of the cataplasma matrix was optimized in the orthogonal method as:gellan gum 0.4 g,xanthan gum 0.03 g,konjac glue 0.1 g,glycerin 4 g,Gluco-Adhesive T(GAT)6 g,Gluco-Adhesive E(GAE)6 g,polyacrylic acid sodium 0.22 g,and sorbitol 3 g.The 180°peel strength,the tensile strength and the elongation at break was 3.043 N,0.275 MPa and 91.05%,respectively.Furthermore,the drug-compatibilities of the matrix were investigated with baicalin,berberine and curcumin,which were used as the models of hydrophilic,poor-water-soluble and hydrophobic ingredients.The drug contents could reach 4.12%,2.42%and 3.75%,while the in vitro release rate were measured as,361.79,55.85 and 104.41μg·cm^(-2)·h^(-1)for baicalin,berberine and curcumin,respectively.These results indicated that the obtained matrix had good drug-compatibility and drug-release properties for different ingredients.
文摘为了研究治伤巴布剂对大鼠早期骨折愈合及对肿瘤坏死因子-α(tumornecrosisfactor-α,TNF-α)表达的影响,将45只SPF级雄性大鼠随机分为空白组、模型组、治伤巴布剂组3个组,每组15只。空白组不造模,其余2组予以闭合造模,造模成功后治伤巴布剂组在骨折标记部位予治伤巴布剂局部外敷;模型组则予等剂量赋形剂局部外敷(每天换药一次),干预7 d。造模后分别于1、3、7 d 3个时相点观测大鼠骨折肢体肿胀度、苏木精-伊红染色法(HE)观察骨折断端病理形态变化、酶联免疫吸附试验(ELISA)检测血清中TNF-α水平。结果表明:(1)与空白组对比,模型组及治伤巴布剂组在1、3、7 d 3个时相点大鼠右侧股骨明显肿胀(P<0.01),治伤巴布剂组在1、3、7 d 3个时相点肢体肿胀度较模型组明显减轻(P<0.01)。(2)HE染色结果显示模型组中可见少量软骨细胞和肉芽组织,未见骨小梁形成;治伤巴布剂可见大量软骨细胞和肉芽组织,并可见少量骨小梁形成。(3)ELISA结果显示与空白组相比,模型组及治伤巴布剂组TNF-α水平明显升高(P<0.01);与模型组相比,治伤巴布剂组TNF-α水平明显降低(P<0.01)。治伤巴布剂可以减轻大鼠股骨中段的肿胀度,对骨折的早期愈合具有一定促进作用,且降低TNF-α浓度水平抑制炎症反应。