AIM:To study our novel caudal approach laparoscopic posterior-sectionectomy with parenchymal transection prior to mobilization under laparoscopy-specific view.METHODS:Points of the procedure are:(1) Patients are put i...AIM:To study our novel caudal approach laparoscopic posterior-sectionectomy with parenchymal transection prior to mobilization under laparoscopy-specific view.METHODS:Points of the procedure are:(1) Patients are put in left lateral position and posterior sector is not mobilized;(2) Glissonian pedicle of the sector is encircled and clamped extra-hepatically and divided afterward during the transection;(3) Dissection of inferior vena cava(IVC) anterior wall behind the liver is started from caudal.Simultaneously,liver transection is performed to search right hepatic vein(RHV) from caudal;(4) Liver transection proceeds to the bifurcation of the vessels from caudal to cranial,exposing the surfaces of IVC and RHV.Since the remnant liver sinks down,the cutting surface is well-opend;and(5) After the completion of transection,dissection of the resected liver from retroperitoneum is easily performed using the gravity.This approach was performed for a 63 years old woman with liver metastasis close to RHV.RESULTS:RHV exposure is required for R0 resection of the lesion.Although the cutting plane is horizontal in supine position and the gravity obstructs the exposure in the small subphrenic space,the use of specific characteristics of laparoscopic hepatectomy,such as the good vision for the dorsal part of the liver and IVC and facilitated dissection using the gravity with the patient positioning,made the complete RHV exposure during the liver transection easy to perform.The operation time was 341 min and operative blood loss was 1356 mL.Her postoperative hospital stay was uneventfull and she is well without any signs of recurrences 14 mo after surgery.CONCLUSION:The new procedure is feasible and useful for the patients with tumors close to RHV and the need of the exposure of RHV.展开更多
Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacteri...Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fiuorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells （MCF-7）. Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.展开更多
【目的】构建用于比较黄曲霉(Aspergillus flavus,A.flavus)菌株之间致病力差异的小鼠感染模型,并利用该模型评价真菌病毒AfPV1对宿主A.flavus致病力的影响。【方法】用不同浓度环磷酰胺腹腔注射Institute of Cancer Research(ICR)小鼠...【目的】构建用于比较黄曲霉(Aspergillus flavus,A.flavus)菌株之间致病力差异的小鼠感染模型,并利用该模型评价真菌病毒AfPV1对宿主A.flavus致病力的影响。【方法】用不同浓度环磷酰胺腹腔注射Institute of Cancer Research(ICR)小鼠,根据白细胞的数量判断小鼠免疫抑制程度;通过滴鼻和尾静脉两种感染方法接种不同浓度的A.flavus孢子量,统计14 d以内小鼠的死亡率,确定A.flavus最佳的孢子接种量;通过小鼠组织的菌落负荷量以及肺部组织的病理观察,确定A.flavus感染是否成功;最后利用该小鼠模型评价真菌病毒AfPV1对寄主A.flavus致病力的影响。【结果】腹腔注射环磷酰胺的浓度为250 mg/kg时,能够达到免疫抑制水平;小鼠组织真菌负荷和病理组织切片观察显示A.flavus成功感染接种的ICR小鼠组织;在滴鼻接种模型中,A.flavus的孢子接种量为40μL(1×10^(6) CFU/mL)时比较合适评价A.flavus菌株之间的差异;在尾静脉接种的模型中,A.flavus的孢子接种量在50μL(1×10^(6) CFU/mL)比较合适评价A.flavus菌株之间的差异;病毒AfPV1能够引起A.flavus的致病力下降,但是不影响A.flavus在小鼠肺部的定殖量。【结论】本研究构建的小鼠感染模型能够评价A.flavus菌株之间致病力的差异;真菌病毒AfPV1的感染降低了宿主A.flavus的致病力。展开更多
文摘AIM:To study our novel caudal approach laparoscopic posterior-sectionectomy with parenchymal transection prior to mobilization under laparoscopy-specific view.METHODS:Points of the procedure are:(1) Patients are put in left lateral position and posterior sector is not mobilized;(2) Glissonian pedicle of the sector is encircled and clamped extra-hepatically and divided afterward during the transection;(3) Dissection of inferior vena cava(IVC) anterior wall behind the liver is started from caudal.Simultaneously,liver transection is performed to search right hepatic vein(RHV) from caudal;(4) Liver transection proceeds to the bifurcation of the vessels from caudal to cranial,exposing the surfaces of IVC and RHV.Since the remnant liver sinks down,the cutting surface is well-opend;and(5) After the completion of transection,dissection of the resected liver from retroperitoneum is easily performed using the gravity.This approach was performed for a 63 years old woman with liver metastasis close to RHV.RESULTS:RHV exposure is required for R0 resection of the lesion.Although the cutting plane is horizontal in supine position and the gravity obstructs the exposure in the small subphrenic space,the use of specific characteristics of laparoscopic hepatectomy,such as the good vision for the dorsal part of the liver and IVC and facilitated dissection using the gravity with the patient positioning,made the complete RHV exposure during the liver transection easy to perform.The operation time was 341 min and operative blood loss was 1356 mL.Her postoperative hospital stay was uneventfull and she is well without any signs of recurrences 14 mo after surgery.CONCLUSION:The new procedure is feasible and useful for the patients with tumors close to RHV and the need of the exposure of RHV.
基金sponsored by grants from the National Natural Science Foundation of China,No.30872603the New Century Excellent Talents Program of the Ministry of Education of China,No.NCET-06-0251the Applied Basic Research Programs of Science and Technology Commission Foundation of Tianjin,China,No.07JCYBJC10200
文摘Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fiuorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells （MCF-7）. Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.
文摘【目的】构建用于比较黄曲霉(Aspergillus flavus,A.flavus)菌株之间致病力差异的小鼠感染模型,并利用该模型评价真菌病毒AfPV1对宿主A.flavus致病力的影响。【方法】用不同浓度环磷酰胺腹腔注射Institute of Cancer Research(ICR)小鼠,根据白细胞的数量判断小鼠免疫抑制程度;通过滴鼻和尾静脉两种感染方法接种不同浓度的A.flavus孢子量,统计14 d以内小鼠的死亡率,确定A.flavus最佳的孢子接种量;通过小鼠组织的菌落负荷量以及肺部组织的病理观察,确定A.flavus感染是否成功;最后利用该小鼠模型评价真菌病毒AfPV1对寄主A.flavus致病力的影响。【结果】腹腔注射环磷酰胺的浓度为250 mg/kg时,能够达到免疫抑制水平;小鼠组织真菌负荷和病理组织切片观察显示A.flavus成功感染接种的ICR小鼠组织;在滴鼻接种模型中,A.flavus的孢子接种量为40μL(1×10^(6) CFU/mL)时比较合适评价A.flavus菌株之间的差异;在尾静脉接种的模型中,A.flavus的孢子接种量在50μL(1×10^(6) CFU/mL)比较合适评价A.flavus菌株之间的差异;病毒AfPV1能够引起A.flavus的致病力下降,但是不影响A.flavus在小鼠肺部的定殖量。【结论】本研究构建的小鼠感染模型能够评价A.flavus菌株之间致病力的差异;真菌病毒AfPV1的感染降低了宿主A.flavus的致病力。
