Objective： To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae （Kle. p） treatment. Methods： We detected the types and expression of cytokines sec...Objective： To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae （Kle. p） treatment. Methods： We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 （TLR4） and the inhibitor factor of κBα （I-κBα） expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay （EMSA）. Results： Cefodizime increased the neutrophils production of TNF-α, IL-β3 and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion： Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p.展开更多
Background Chronic hepatic inflammation is characterized by the accumulation of lymphocytes as a consequence of increased recruitment from the blood and retention within the tissue at sites of infection. CXC chemokine...Background Chronic hepatic inflammation is characterized by the accumulation of lymphocytes as a consequence of increased recruitment from the blood and retention within the tissue at sites of infection. CXC chemokine ligand 16 （CXCL16） mRNA has been detected in both inflamed and normal liver tissues and is strongly upregulated in the injured liver tissues in a murine model. The aim of this study was to investigate the effect of cefodizime on CXCL16 mRNA of liver tissues in mice with immunological hepatic injury.Methods The murine model of immunological hepatic injury was induced by Bacillus Calmette Guerin and Lipoposaccharide. The mice with immunological hepatic injury were randomly assigned to the model group, the cefodizime group and the ceftriaxone group. The three groups were continuously given agents for seven days and CXCL16 mRNA of liver tissue was determined and contrasted with the control group treated by normal saline. Reverse transcription-polymerase chain reaction was used to assay CXCL16 mRNA levels in liver tissues.Results The expressions of CXCL16 mRNA were significantly higher in the model group and the ceftriaxone group than in the control group and the cefodizime group （P〈0.05）, indicating the mice in the model group and the ceftriaxone group were immunodeficient. There was no statistical difference in the expressions of CXCL16 mRNA between the control group and the cefodizime group. Similarly, no statistical difference in the expressions of CXCL16 mRNA between the model group and the ceftriaxone group was detected （P 〉0.05）. Conclusion Cefodizime effectively reduces the infiltration of lymphocytes into liver tissues and alleviates the liver damage by decreasing CXCL16 rnRNA in liver tissues in mice with immunological hepatic injury.展开更多
The induction time of cefodizime sodium was measured in ethanol-water at different solvent composi- tions by the laser technology measurement. The results indicate that the solvent composition played an important role...The induction time of cefodizime sodium was measured in ethanol-water at different solvent composi- tions by the laser technology measurement. The results indicate that the solvent composition played an important role in the supersaturation and the nucleation process of cefodizime sodium solution. According to the modified classical nucleation theory, the nucleation and growth mechanism were identified. The correlation results show that heterogeneous nucleation dominated the nucleation process at lower supersaturation, where homogeneous nucleation is the most important mechanism at higher supersaturation. Based on the correlated results, the 2D mediated growth mechanism had the highest correlation coefficients （R2）, so this mechanism was selected as the proper growth mechanism for cefodizime sodium.展开更多
基金the Natural Science Foundation of GuangdongProvince (5300983)
文摘Objective： To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae （Kle. p） treatment. Methods： We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 （TLR4） and the inhibitor factor of κBα （I-κBα） expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay （EMSA）. Results： Cefodizime increased the neutrophils production of TNF-α, IL-β3 and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion： Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p.
文摘Background Chronic hepatic inflammation is characterized by the accumulation of lymphocytes as a consequence of increased recruitment from the blood and retention within the tissue at sites of infection. CXC chemokine ligand 16 （CXCL16） mRNA has been detected in both inflamed and normal liver tissues and is strongly upregulated in the injured liver tissues in a murine model. The aim of this study was to investigate the effect of cefodizime on CXCL16 mRNA of liver tissues in mice with immunological hepatic injury.Methods The murine model of immunological hepatic injury was induced by Bacillus Calmette Guerin and Lipoposaccharide. The mice with immunological hepatic injury were randomly assigned to the model group, the cefodizime group and the ceftriaxone group. The three groups were continuously given agents for seven days and CXCL16 mRNA of liver tissue was determined and contrasted with the control group treated by normal saline. Reverse transcription-polymerase chain reaction was used to assay CXCL16 mRNA levels in liver tissues.Results The expressions of CXCL16 mRNA were significantly higher in the model group and the ceftriaxone group than in the control group and the cefodizime group （P〈0.05）, indicating the mice in the model group and the ceftriaxone group were immunodeficient. There was no statistical difference in the expressions of CXCL16 mRNA between the control group and the cefodizime group. Similarly, no statistical difference in the expressions of CXCL16 mRNA between the model group and the ceftriaxone group was detected （P 〉0.05）. Conclusion Cefodizime effectively reduces the infiltration of lymphocytes into liver tissues and alleviates the liver damage by decreasing CXCL16 rnRNA in liver tissues in mice with immunological hepatic injury.
文摘The induction time of cefodizime sodium was measured in ethanol-water at different solvent composi- tions by the laser technology measurement. The results indicate that the solvent composition played an important role in the supersaturation and the nucleation process of cefodizime sodium solution. According to the modified classical nucleation theory, the nucleation and growth mechanism were identified. The correlation results show that heterogeneous nucleation dominated the nucleation process at lower supersaturation, where homogeneous nucleation is the most important mechanism at higher supersaturation. Based on the correlated results, the 2D mediated growth mechanism had the highest correlation coefficients （R2）, so this mechanism was selected as the proper growth mechanism for cefodizime sodium.
