Objective: The tumor tissues used in molecular pathological detection were usually obtained by surgery, which would cause trauma and may not be suitable for the terminal cancer patients. This paper evaluated the value...Objective: The tumor tissues used in molecular pathological detection were usually obtained by surgery, which would cause trauma and may not be suitable for the terminal cancer patients. This paper evaluated the value of the non-small cell lung cancer(NSCLC) pleural effusion cell blocks as tumor tissues replacement materials in the application of molecular pathological detection. Methods: Tumor cells were made into cell blocks through stratified centrifugal from 30 NSCLC patients with the pleural effusion. The immunohistochemistry, fluorescence in situ hybridization(FISH) and gene sequencing methods were employed in our experiments. Results: The tumor cells of cell block section were rich and could keep part of histological structure. Immunohistochemistry staining could assist diagnosis and tumor parting. Epidermal growth factor receptor(EGFR) FISH-positive was found in 33.33% of the group, high polysomy in 6 cases, amplification in 4 cases. EGFR gene mutations were found in 8 cases of 30 samples, with an incidence of 26.67%, 6 cases were detected in the exon 19, and 2 cases were detected in the exon 21. Conclusion: The NSCLC pleural effusion cell blocks are useful for the diagnosis and determining the primary source of tumor, instructed targeted therapy.展开更多
Programmed cell death （PCD） signaling pathways are import- ant contributors to acute neurological insults such as hypox- ic-ischemic brain damage, traumatic brain injury, stroke etc. The pathogenesis of all these di...Programmed cell death （PCD） signaling pathways are import- ant contributors to acute neurological insults such as hypox- ic-ischemic brain damage, traumatic brain injury, stroke etc. The pathogenesis of all these diseases is closely linked with ab- erration of apoptotic cell death pathways. Mitochondria play a crucial role during PCD, acting as both sensors of death signals, and as initiators of biochemical path- ways, which cause cell death （Bras et al., 2005）. Cytochrome c was the firstly identified apoptogenic factor released from mitochondria into the cytosol, where it induces apoptosome formation through the activation of caspases. Other proteins, such as apoptosis inducing factor （AIF）, have been subsequently identified as mitochondrial released factors. AIF contributes to apoptotic nuclear DNA damage （Bras et al., 2005）. in a caspase-independent way展开更多
Endoscopic transpapillary brush cytology and forceps biopsy during endoscopic retrograde cholangiopancreatology are generally used to obtain pathological evidence of biliary strictures. Recently, the new endoscopic sc...Endoscopic transpapillary brush cytology and forceps biopsy during endoscopic retrograde cholangiopancreatology are generally used to obtain pathological evidence of biliary strictures. Recently, the new endoscopic scraper Trefle~? has been reported and demonstrated high cancer detectability in malignant biliary strictures. This device is used to scrape the stricture over the guidewire, and, in the original method, the tissue and/or cell samples obtained are subjected to histological and/or cytological analysis separately. However, discrimination of chunks of tissue is hampered by the opacity of the surrounding fluid. We have developed a cell block technique for the Trefle~? device without dividing obtained specimens into tissue and cellular components, which is the simplest method and enables immunohistochemical analysis. We present a case of obstructive jaundice diagnosed immunohistochemically as pancreatic metastasis from colon cancer using cell block sections obtained with the Trefle~? device, which procedure is as easy as conventional brush cytology.展开更多
Aim To Put forward an improve algorithm for real-time connection admission control (CAC) decision. Methods converted equations of Cell loss ratio (CLR) upper bound were derived based on typical approaches to reduce co...Aim To Put forward an improve algorithm for real-time connection admission control (CAC) decision. Methods converted equations of Cell loss ratio (CLR) upper bound were derived based on typical approaches to reduce computing load. Moreover, the effects of usage parameter control (UPC) policing and bandwidth allocation functions were combined to deduce improver equations. computing load and admission region of the new algorithm were analyzed, while numerical examples and comparisons with classical non-parametic method were also included. Results Computing burden was reduced. More accurate estimation of CLR was obtained. The admission region of new algorithm was larger than typical non-parametric approach and was variable according to the burst length.Conclusion The calculation burden of new algorithm for CLR estimation is lighter than classical non-parametric approach. In addition to that, just as many information as what we can get in connection setup process are used for CAC decision. So it is not only a practicable but also an effective method that can be used in real-time processing.展开更多
Visible light positioning （VLP） is an emerging candidate for indoor positioning, which carl simultaneously meet the requirements for accuracy, cost, coverage area, and security. However, intercell interference causc...Visible light positioning （VLP） is an emerging candidate for indoor positioning, which carl simultaneously meet the requirements for accuracy, cost, coverage area, and security. However, intercell interference causcd by light intensity superposition linfits the application of VLP. In this Letter, we propose a united block sequence mapping （UBSM）-based VLP that utilizes superposition to integrate the multidimensional information from dense small cells into 2D information. The experimental result shows that UBSM-based VLP can achieve an accuracy of 1.5 cm with a 0.4 m row spacing and 0.35 m column spacing of LED lights.展开更多
文摘Objective: The tumor tissues used in molecular pathological detection were usually obtained by surgery, which would cause trauma and may not be suitable for the terminal cancer patients. This paper evaluated the value of the non-small cell lung cancer(NSCLC) pleural effusion cell blocks as tumor tissues replacement materials in the application of molecular pathological detection. Methods: Tumor cells were made into cell blocks through stratified centrifugal from 30 NSCLC patients with the pleural effusion. The immunohistochemistry, fluorescence in situ hybridization(FISH) and gene sequencing methods were employed in our experiments. Results: The tumor cells of cell block section were rich and could keep part of histological structure. Immunohistochemistry staining could assist diagnosis and tumor parting. Epidermal growth factor receptor(EGFR) FISH-positive was found in 33.33% of the group, high polysomy in 6 cases, amplification in 4 cases. EGFR gene mutations were found in 8 cases of 30 samples, with an incidence of 26.67%, 6 cases were detected in the exon 19, and 2 cases were detected in the exon 21. Conclusion: The NSCLC pleural effusion cell blocks are useful for the diagnosis and determining the primary source of tumor, instructed targeted therapy.
文摘Programmed cell death （PCD） signaling pathways are import- ant contributors to acute neurological insults such as hypox- ic-ischemic brain damage, traumatic brain injury, stroke etc. The pathogenesis of all these diseases is closely linked with ab- erration of apoptotic cell death pathways. Mitochondria play a crucial role during PCD, acting as both sensors of death signals, and as initiators of biochemical path- ways, which cause cell death （Bras et al., 2005）. Cytochrome c was the firstly identified apoptogenic factor released from mitochondria into the cytosol, where it induces apoptosome formation through the activation of caspases. Other proteins, such as apoptosis inducing factor （AIF）, have been subsequently identified as mitochondrial released factors. AIF contributes to apoptotic nuclear DNA damage （Bras et al., 2005）. in a caspase-independent way
文摘Endoscopic transpapillary brush cytology and forceps biopsy during endoscopic retrograde cholangiopancreatology are generally used to obtain pathological evidence of biliary strictures. Recently, the new endoscopic scraper Trefle~? has been reported and demonstrated high cancer detectability in malignant biliary strictures. This device is used to scrape the stricture over the guidewire, and, in the original method, the tissue and/or cell samples obtained are subjected to histological and/or cytological analysis separately. However, discrimination of chunks of tissue is hampered by the opacity of the surrounding fluid. We have developed a cell block technique for the Trefle~? device without dividing obtained specimens into tissue and cellular components, which is the simplest method and enables immunohistochemical analysis. We present a case of obstructive jaundice diagnosed immunohistochemically as pancreatic metastasis from colon cancer using cell block sections obtained with the Trefle~? device, which procedure is as easy as conventional brush cytology.
文摘Aim To Put forward an improve algorithm for real-time connection admission control (CAC) decision. Methods converted equations of Cell loss ratio (CLR) upper bound were derived based on typical approaches to reduce computing load. Moreover, the effects of usage parameter control (UPC) policing and bandwidth allocation functions were combined to deduce improver equations. computing load and admission region of the new algorithm were analyzed, while numerical examples and comparisons with classical non-parametic method were also included. Results Computing burden was reduced. More accurate estimation of CLR was obtained. The admission region of new algorithm was larger than typical non-parametric approach and was variable according to the burst length.Conclusion The calculation burden of new algorithm for CLR estimation is lighter than classical non-parametric approach. In addition to that, just as many information as what we can get in connection setup process are used for CAC decision. So it is not only a practicable but also an effective method that can be used in real-time processing.
基金supported in part by the National 973Program of China(No.2013CB329205)the National Science Foundation of China(No.61401032)
文摘Visible light positioning （VLP） is an emerging candidate for indoor positioning, which carl simultaneously meet the requirements for accuracy, cost, coverage area, and security. However, intercell interference causcd by light intensity superposition linfits the application of VLP. In this Letter, we propose a united block sequence mapping （UBSM）-based VLP that utilizes superposition to integrate the multidimensional information from dense small cells into 2D information. The experimental result shows that UBSM-based VLP can achieve an accuracy of 1.5 cm with a 0.4 m row spacing and 0.35 m column spacing of LED lights.
