Objective: To study the effects of 24 Chinese medicinal herbs on nucleic acid, protein and cellcycle of human lung adenocarcinoma cells. Methods: Using flow cytometry the authors analysed the effects of 24Chinese medi...Objective: To study the effects of 24 Chinese medicinal herbs on nucleic acid, protein and cellcycle of human lung adenocarcinoma cells. Methods: Using flow cytometry the authors analysed the effects of 24Chinese medicinal herbs (CMH) in compound recipe on proliferation index (PI), DNA index, protein index andratio of various phases in cell cycle of human lung adenocarcinoma cells (SPC-A-1). Results: The PI was morethan 20%, in 4 CMH, while 3 CMH, Gynostemma Pentaphylla, Radix Glehnia, and Radix Ginseng couldstrengthen the body resistance. That suggested using CMH of strengthening body resistance could not only serveas conventional ionic but also as tumor cell inhibitor. Meanwhile the action points of 24 CMH on cell cycle weredifferent from one another. Conclusions: According to these results, Chinese recipes were more effective in clinical practice.展开更多
From the first application of the Boolean model to the cell cycle regulation network of budding yeast,new regulative pathways have been discovered,par-ticularly in the G1/S transition circuit.This discovery called for...From the first application of the Boolean model to the cell cycle regulation network of budding yeast,new regulative pathways have been discovered,par-ticularly in the G1/S transition circuit.This discovery called for finer modeling to study the essential biology,and the resulting outcomes are first introduced in the ar-ticle.A traditional Boolean network model set up for the new G1/S transition circuit shows that it cannot correctly simulate real biology unless the model parameters are fine tuned.The deficiency is caused by an overly coarse-grained description of the inhibitor binding process,which shall be overcome by a two-vector model proposed whose robustness is surveyed using random perturba-tions.Simulations show that the proposed two-vector model is much more robust in describing inhibitor binding processes within the Boolean framework.展开更多
文摘Objective: To study the effects of 24 Chinese medicinal herbs on nucleic acid, protein and cellcycle of human lung adenocarcinoma cells. Methods: Using flow cytometry the authors analysed the effects of 24Chinese medicinal herbs (CMH) in compound recipe on proliferation index (PI), DNA index, protein index andratio of various phases in cell cycle of human lung adenocarcinoma cells (SPC-A-1). Results: The PI was morethan 20%, in 4 CMH, while 3 CMH, Gynostemma Pentaphylla, Radix Glehnia, and Radix Ginseng couldstrengthen the body resistance. That suggested using CMH of strengthening body resistance could not only serveas conventional ionic but also as tumor cell inhibitor. Meanwhile the action points of 24 CMH on cell cycle weredifferent from one another. Conclusions: According to these results, Chinese recipes were more effective in clinical practice.
基金supported by the Chinese Ministry of Science and Technology(2003CB715900)the National Natural Science Foundation of China(Grant No.20640120446).
文摘From the first application of the Boolean model to the cell cycle regulation network of budding yeast,new regulative pathways have been discovered,par-ticularly in the G1/S transition circuit.This discovery called for finer modeling to study the essential biology,and the resulting outcomes are first introduced in the ar-ticle.A traditional Boolean network model set up for the new G1/S transition circuit shows that it cannot correctly simulate real biology unless the model parameters are fine tuned.The deficiency is caused by an overly coarse-grained description of the inhibitor binding process,which shall be overcome by a two-vector model proposed whose robustness is surveyed using random perturba-tions.Simulations show that the proposed two-vector model is much more robust in describing inhibitor binding processes within the Boolean framework.
