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In situ forming injectable MSC-loaded GelMA hydrogels combined with PD for vascularized sweat gland regeneration
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作者 Enhe Jirigala Bin Yao +11 位作者 Zhao Li Yi-Jie Zhang Chao Zhang Li-Ting Liang Fan-Liang Zhang Xing-Yu Yuan Xian-Lan Duan Wei Song Meng-De Zhang Yi Kong Xiao-Bing Fu Sha Huang 《Military Medical Research》 SCIE CAS CSCD 2024年第1期152-155,共4页
Dear Editor,Three dimensional(3D)bioprinted extracellular matrix(ECM)can be used to provide both biochemical and biophysical cues to direct mesenchymal stem cells(MSCs)differentiation,and then differentiated cells wer... Dear Editor,Three dimensional(3D)bioprinted extracellular matrix(ECM)can be used to provide both biochemical and biophysical cues to direct mesenchymal stem cells(MSCs)differentiation,and then differentiated cells were isolated for implantation in vivo using surgical procedures.However,the reduced cell activity after cell isolation from 3D constructs and low cell retention in injured sites limit its application[1].Methacrylated gelatin(GelMA)hydrogel has the advantage of fast crosslinking,which could resemble complex architectures of tissue construct in vivo[2].Here,we adopted a noninvasive bioprinting procedure to imitate the regenerative microenvironment that could simultaneously direct the sweat gland(SG)and vascular differentiation from MSCs and ultimately promote the replacement of glandular tissue in situ(Fig.1a). 展开更多
关键词 sweat gland GelMA In situ niche Cell differentiation Tissue incorporation VASCULARIZATION
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Comparison of biological behavior of lacrimal gland adenoid cystic carcinoma with high-grade transformation cells 被引量:2
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作者 Chuan-Li Zhang Li-Min Zhu +3 位作者 Xun Liu Mei-Xia Jiang Ting-Ting Lin Yan-Jin He 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第2期163-171,共9页
AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformati... AIM:To evaluate the differences between human lacrimal gland adenoid cystic carcinoma with high-grade transformation(LACC-HGT)primar y cells cultured by high-grade transformation tissue and non-high-grade transformation(non-HGT)primary cells cultured by non-highgrade transformation tissue in proliferation,metastasis,drug susceptibility,and genes.METHODS:LACC-HGT primary cells were established by tissue block culture,and the 4^(th)to 10^(th)generation primary cells were selected as research objects.The cells were preliminarily identified by immunofluorescent staining.The differences between non-HGT and LACC-HGT primary cells in terms of proliferation,metastasis,and drug susceptibility were compared by cell counting kit-8(CCK-8)assay,wound healing,and drug sensitivity experiments.Differentially expressed genes were screened using mRNA array.Gene expression was analyzed using real-time quantitative polymerase chain reaction(RT-qPCR).RESULTS:LACC-HGT primary cells were successfully cultured by tissue block culture.Immunofluorescence staining results showed that cytokeratin(CK)and CK7 expression levels were positive in LACC-HGT primary cells.CCK-8 results showed that the proliferation ability of LACCHGT cells was significantly higher than that of non-HGT cells.Wound healing experiment showed that the migration ability of LACC-HGT cells was significantly higher than that of non-HGT cells.LACC-HGT cells were also less sensitive to cisplatin and paclitaxel than non-HGT cells.Compared with non-HGT cells,9566 differentially expressed genes were found in LACC-HGT primary cells,of which 5162 were upregulated and 4404 were down-regulated.The expression of N-acetylneuraminate pyruvate lyase(NPL),MARVEL domain containing 3(MARVELD3),syntabulin(SYBU),and allograft inflammatory factor 1(AIF1)was higher in LACCHGT cells than in non-HGT cells,whereas that of periostin(POSTN)was lower.CONCLUSION:LACC-HGT primary cells have faster proliferation,stronger migration ability,and poorer sensitivity to chemotherapy drugs than non-HGT primary cells.The expression of mRNAs in non-HGT and LACC-HGT primary cells are significantly different.These features are speculated to be the reasons why high-grade transformation tissues exhibit higher malignant degree and poorer prognosis than their counterparts. 展开更多
关键词 lacrimal gland lacrimal gland adenoid cystic carcinoma high-grade transformation primary cell culture biological behavior mRNA array
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Conversion of Adipose-Derived Stem Cells into Sweat Gland-Like Cells: An In Vitro Phenotypic Study
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作者 Shuai Qiang Xiao-Meng Wang +5 位作者 Cheng-Kun Zhang Ying Liu Zhi-Bo Xiao Qiang Li Feng-Yong Li Yu Zhou 《International Journal of Dermatology and Venereology》 2020年第3期166-170,共5页
Objective:This study was performed to explore the conversion of adipose-derived stem cells(ADSCs)into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland(hSG)cells we... Objective:This study was performed to explore the conversion of adipose-derived stem cells(ADSCs)into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland(hSG)cells were isolated,cultured,and identified.The ADSCs were then cultured in combination with epidermal growth factor and/or cocultured with hSG cells in a Transwell coculturing system to transform the ADSCs into hSG-like cells.Phenotypic changes of the ADSCs were examined by morphological observation and immunocytochemical analysis of specific markers.Results:The ADSCs showed sweat gland-like morphologic changes and expressed sweat gland markers(cytokeratins 7,14,and 18).Conclusion:These findings revealed that ADSCs can differentiate into hSG-like cells after coculture in a Transwell system and that epidermal growth factor can enhance the efficiency of differentiation.ADSCs may serve as a potential source of cells for sweat gland regeneration. 展开更多
关键词 adipose-derived stem cells human sweat gland-like cells DIFFERENTIATION
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Influence on Cellular Signal Transduction Pathway in Dairy Cow Mammary Gland Epithelial Cells by Galactopoietic Compound Isolated from Vaccariae segetalis 被引量:11
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作者 WAN Zhong-ying TONG Hui-li LI Qing-zhang GAO Xue-jun 《Agricultural Sciences in China》 CAS CSCD 2011年第4期619-630,共12页
The galactopoietic mechanism of Vaccaria segetalis is still unknown. Understanding dibutyl phthalate (DBP) separated from Vaccaria segetalis on the expression of lactation signal transduction genes of mammary gland ... The galactopoietic mechanism of Vaccaria segetalis is still unknown. Understanding dibutyl phthalate (DBP) separated from Vaccaria segetalis on the expression of lactation signal transduction genes of mammary gland epithelial cells, including prlr, erα, akt1, socs2, pparγ and elf5, will be helpful to reveal the molecular mechanism. Western blot and qRT- PCR were used to study the change of prlr, erα, akt, socs2, pparγ, and elf5 expression at mRNA and protein level. Co- localization expression of prolactin receptor (PRLR) and estrogen receptor α (ERα) was observed by immunofluorescence; the expression changes of miRNAs (21, 125b, 143, and 195) and the secretion of β-casein and lactose were detected by qRT-PCR and RP-HPLC. The results showed that Vaccaria segetalis active compound had similar fuctions as estrogen and/or prolactin (PRL) in dairy cow mammary gland epithelial cells (DCMECs), increased the expressions of prlr, erα, akt1, and elf5 genes, while repressed pparγ expressions. DBP promoted socs2 mRNA expression, but its protein expressions were repressed. Furthermore, both DBP and PRL could repress the expressions of miRNA-125b, miRNA-143 and miRNA- 195 in DCMECs. DBP could repress the expression of miRNA-21, while the influence of PRL on miRNA-21 was not certain. DBP could promote the lactation ability of DCMECs by regulating the ER and PRLR cellular signal transduction pathway. 展开更多
关键词 Vaccaria segetalis DBP dairy cow mammary gland epithelial cells signal transduction MIRNAS
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Bone marrow-derived mesenchymal stem cells migrate to healthy and damaged salivary glands following stem cell infusion 被引量:7
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作者 Silke Schwarz Ralf Huss +4 位作者 Michaela Schulz-Siegmund Breda Vogel Sven Brandau Stephan Lang Nicole Rotter 《International Journal of Oral Science》 SCIE CAS CSCD 2014年第3期154-161,共8页
Xerostomia is a severe side effect of radiation therapy in head and neck cancer patients. To date, no satisfactory treatment option has been established. Because mesenchymal stem cells(MSCs) have been identified as ... Xerostomia is a severe side effect of radiation therapy in head and neck cancer patients. To date, no satisfactory treatment option has been established. Because mesenchymal stem cells(MSCs) have been identified as a potential treatment modality, we aimed to evaluate stem cell distribution following intravenous and intraglandular injections using a surgical model of salivary gland damage and to analyse the effects of MSC injections on the recruitment of immune cells. The submandibular gland ducts of rats were surgically ligated. Syngeneic adult MSCs were isolated, immortalised by simian virus 40(SV40) large T antigen and characterized by flow cytometry. MSCs were injected intravenously and intraglandularly. After 1, 3 and 7 days, the organs of interest were analysed for stem cell recruitment. Inflammation was analysed by immunohistochemical staining. We were able to demonstrate that, after intravenous injection, MSCs were recruited to normal and damaged submandibular glands on days 1, 3 and 7. Unexpectedly, stem cells were recruited to ligated and non-ligated glands in a comparable manner. After intraglandular injection of MSCs into ligated glands, the presence of MSCs, leucocytes and macrophages was enhanced, compared to intravenous injection of stem cells. Our data suggest that injected MSCs were retained within the inflamed glands, could become activated and subsequently recruited leucocytes to the sites of tissue damage. 展开更多
关键词 duct ligation mesenchymal stem cells submandibular gland injury XEROSTOMIA
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Improved sweat gland function during active heating in tennis athletes
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作者 Jeong-Beom Lee Soon-Bok Na Tae-Wook Kim 《Journal of Sport and Health Science》 SCIE 2016年第4期443-447,共5页
Background:Relatively few studies on the peripheral sweating mechanisms of trained tennis athletes have been conducted.The purpose of this study was to compare the sweating capacities of tennis athletes against untra... Background:Relatively few studies on the peripheral sweating mechanisms of trained tennis athletes have been conducted.The purpose of this study was to compare the sweating capacities of tennis athletes against untrained subjects(controls).Methods:Thirty-fi e healthy male volunteers participated including 15 untrained subjects and 20 trained tennis athletes(nationally ranked).Active heat generation was performed for 30 min(running at 60%VO2max) in a climate chamber(temperature,25.0°C ± 0.5°C;relative humidity,60% ± 3%,termed active heating).Sweating data(local sweat onset time,local sweat volume,activated sweat glands,sweat output per gland,whole body sweat loss volume) were measured by the capacitance hygrometer-ventilated capsule method and starch-iodide paper.Mean body temperature was calculated from tympanic and skin temperatures.Results:Local sweat onset time was shorter for tennis athletes(p〈0.001).Local sweat volume,activated sweat glands of the torso and limbs,sweat output per gland,and whole body sweat loss volume were significant y higher for tennis athletes than control subjects after active heating(p〈0.001).Tympanic and mean body temperatures were lower among tennis athletes than controls(p〈0.05).Conclusion:These results indicate that tennis athletes had increased regulatory capacity of their sweat gland function. 展开更多
关键词 Activated sweat glands Active heating sweat onset time sweat output sweating function Tennis athletes
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Comparison on In-Vitro Culture Methods of Yak Endometrial Gland Epithelial Cells
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作者 WU Qing-xia DONG Hal-long RUI Ya-pei 《Animal Husbandry and Feed Science》 CAS 2011年第4期9-12,共4页
[ Objective] To develop in-vitro culture methods of yak endometrial gland epithelial cells. [Method] The gland epithelial cells were isolated from yak endometrium by explant culture method and digestion culture method... [ Objective] To develop in-vitro culture methods of yak endometrial gland epithelial cells. [Method] The gland epithelial cells were isolated from yak endometrium by explant culture method and digestion culture method, respectively. [ Result] In the first method, the cells isolated from the endometrium explant could merge into monolayer after 8-d culture, and they could be purified by gradation digestion with trypsin. In the second method, the endometrium explant were first digested by collagenase II by incubation at 37℃ for 2.5 h and then further digested in fresh 2 g/L colla- genase II for another 2.5 h. The cell suspension was leached through 74 pm filter and centrifuged at 400 r/min for 5 min. Then the cell pellet was re-suspended, followed by natural sedimentation to collect purified gland epithelial cells. The isolated cells were cytokeratin-positive as detected by immunocytochemical staining, and the positive rate could reach 95%. [Conclusion] The yak endometrial gland epithelial cells can be isolated and purified by both the explant culture method and digestion culture method. 展开更多
关键词 YAK ENDOMETRIUM gland epithelial cells In-vitro culture
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Calcium Participates in Secretion of Porphyrin from Shell Gland Epithelial Cells of Japanese Quail (<i>Coturnix coturnix japonica</i>)
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作者 Osamu Kai 《Open Journal of Animal Sciences》 2019年第1期151-161,共11页
To investigate factors involved in the secretion of protoporphyrin IX (PpIX), a superficial eggshell pigment, from shell gland epithelial cells of Japanese quail, we cultured cells in Ham’s F12 medium with calcium ch... To investigate factors involved in the secretion of protoporphyrin IX (PpIX), a superficial eggshell pigment, from shell gland epithelial cells of Japanese quail, we cultured cells in Ham’s F12 medium with calcium chloride and quail plasma. The addition of hormones (prostaglandin F2α, progesterone, estradiol-17β) to the medium did not change the PpIX concentration in the culture supernatant, but changing the calcium chloride (CaCl2) concentration did: a lower concentration of CaCl2 led to a higher PpIX concentration;0 mM CaCl2 enhanced the secretion of PpIX from epithelial cells prepared at 5 or 7 mM CaCl2. The result suggests that a drop in concentration of CaCl2 mimics the end of shell calcification and stimulates rapid secretion of PpIX in vivo. Bovine serum albumin was almost as effective as quail plasma for PpIX secretion in culture, and would facilitate further study of the mechanism of PpIX secretion. 展开更多
关键词 Japanese Quail SHELL gland Epithelial cells In Vitro Culture CALCIUM Chloride PROTOPORPHYRIN
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<i>In Vitro</i>Culture of Shell Gland Epithelial Cells in Japanese Quail (<i>Coturnix coturnix japonica</i>)
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作者 Osamu Kai Kaoru Mineo 《Open Journal of Animal Sciences》 2018年第2期184-190,共7页
To investigate the secretion of protoporphyrin IX (PpIX), superficial eggshell pigment, from shell gland cells of Japanese quail, the epithelial cells of the gland were collected and isolated for cultivation in vitro.... To investigate the secretion of protoporphyrin IX (PpIX), superficial eggshell pigment, from shell gland cells of Japanese quail, the epithelial cells of the gland were collected and isolated for cultivation in vitro. An analysis of a peak for PpIX in the cells was performed using a fluorescence microplate reader. The measurement showed that PpIX has a peak of excitation wavelength at 410 nm and emission wavelength at 606 nm in the culture medium (HamF12 + 4% HCl). Volumes of PpIX in the medium after 4 hour culture of the cells were measured with a microplate reader using filter set of excitation wavelength 400/30nm and emission wavelength 620/40nm. However the cells did not secrete significantly PpIX during 4 hour incubation in this culture system, addition of quail plasma to the medium resulted in significantly higher secretion. A cultivation system in this study is able to use for the study on the mechanism of the secretion of eggshell pigment, PpIX from Japanese quail shell gland epithelial cells. 展开更多
关键词 Japanese Quail SHELL gland Epithelial cells In Vitro CULTURE Protoporphyrin Fluorescence Microplate Reader
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Transplantation of human bone marrow-derived mesenchymal stem cells transfected with ectodysplasin for regeneration of sweat glands 被引量:20
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作者 CAI Sa PAN Yu +3 位作者 HAN Bing SUN Tong-zhu SHENG Zhi-yong FU Xiao-bing 《Chinese Medical Journal》 SCIE CAS CSCD 2011年第15期2260-2268,共9页
Background Patients with severe full-thickness burn injury suffer from their inability to maintain body temperature through perspiration because the complete destructed sweat glands can not be regenerated. Bone marrow... Background Patients with severe full-thickness burn injury suffer from their inability to maintain body temperature through perspiration because the complete destructed sweat glands can not be regenerated. Bone marrow-derived mesenchymal stem cells (BM-MSCs) represent an ideal stem-cell source for cell therapy because of their easy purification and multipotency. In this study, we attempted to induce human BM-MSCs to differentiate into sweat gland cells for sweat gland regeneration through ectodysplasin (EDA) gene transfection. Methods The dynamic expression of EDA and EDA receptor (EDAR) were firstly observed in the sweat gland formation during embryological development. After transfection with EDA expression vector, human BM-MSCs were transplanted into the injured areas of burn animal models. The regeneration of sweat glands was identified by perspiration test and immunohistochemical analysis. Results Endogenous expression of EDA and EDAR correlated with sweat gland development in human fetal skin. After EDA transfection, BM-MSC acquired a sweat-gland-cell phenotype, evidenced by their expression of sweat gland markers by flow cytometry analysis. Immunohistochemical staining revealed a markedly contribution of EDA-transfected BM-MSCs to the regeneration of sweat glands in the scalded paws. Positive rate for perspiration test for the paws treated with EDA-transfected BM-MSCs was significantly higher than those treated with BM-MSCs or EDA expression vector (P 〈0.05). Conclusions Our results confirmed the important role of EDA in the development of sweat gland. BM-MSCs transfected with EDA significantly improved the sweat-gland regeneration. This study suggests the potential application of EDA-modified MSCs for the repair and regeneration of injured skin and its appendages. 展开更多
关键词 bone marrow-derived mesenchymal stem cells sweat gland ECTODYSPLASIN REGENERATION
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Future application of hair follicle stem cells: capable in differentiation into sweat gland cells 被引量:9
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作者 WANG Yao LIU Zhi-yue +3 位作者 ZHAO Qing SUN Tong-zhu MA Kui FU Xiao-bing 《Chinese Medical Journal》 SCIE CAS CSCD 2013年第18期3545-3552,共8页
Background Sweat glands (SGs) can not regenerate after complete destruction in the severe skin injury, so it is important to find a ideal stem cell source in order to regenerate functional SGs. Hair follicle stem ce... Background Sweat glands (SGs) can not regenerate after complete destruction in the severe skin injury, so it is important to find a ideal stem cell source in order to regenerate functional SGs. Hair follicle stem cells (HFSCs) possess the obvious properties of the adult stem cells, which are multipotent and easily accessible. In this research, we attempted to direct the HFSCs suffered from the sweat gland cells (SGCs) special differentiation by a cooperative co- culture system in vitro. Methods The designed co-culture microenvironment in the transwell was consist of two critial factors: heat shocked SGCs and dermis-like mesenchymal tissue, which appeared independently in the two control groups; after induction, the purified induced SGC-like cells were transplanted into the full-thickness scalded wounds of the nude mice, after 4 weeks, the reconstructed SG-like structures were identified by immunohistochemical and immunofluorescence analysis. Results A part of HFSCs in experimental group finally expressed SGCs phenotypes, by contrast, the control group 1 which just containing dermis-like mesenchymal tissue failed and the control group 2 consisted of heat shocked SGCs was in a poor efficiency; by immunofluorescence staining and flow cytometry analysis, the expression of HFSCs special biomarkers was down regulated, instead of the positive efficiency of SGCs special antigens increased; besides, the induced SGCs displayed a high expression of ectodysplasin A (EDA) and ectodysplasin A receptor (EDAR) genes and proteins; after cell transplantation, the youngest SG-like structures formed and be positive in SGCs special antigens, which never happened in untreated wounds (P 〈0.05). Conclusion The HFSCs are multipotential and capable in differentiating into SGCs which promise a potential stem cells reservoir for future use; our special co-culture microenvironment is promising for HFSCs differentiating; the induced SGCs are functional and could work well in the regeneration of SGs. 展开更多
关键词 sweat gland hair follicle stem cells INDUCTION DIFFERENTIATION REGENERATION
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Redirecting differentiation of mammary progenitor cells by 3D bioprinted sweat gland microenvironment 被引量:9
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作者 Rui Wang Yihui Wang +8 位作者 Bin Yao Tian Hu Zhao Li Yufan Liu Xiaoli Cui Liuhanghang Cheng Wei Song Sha Huang Xiaobing Fu 《Burns & Trauma》 SCIE 2019年第1期269-278,共10页
Background:Mammary progenitor cells(MPCs)maintain their reproductive potency through life,and their specific microenvironments exert a deterministic control over these cells.MPCs provides one kind of ideal tools for s... Background:Mammary progenitor cells(MPCs)maintain their reproductive potency through life,and their specific microenvironments exert a deterministic control over these cells.MPCs provides one kind of ideal tools for studying engineered microenvironmental influence because of its accessibility and continually undergoes postnatal developmental changes.The aim of our study is to explore the critical role of the engineered sweat gland(SG)microenvironment in reprogramming MPCs into functional SG cells.Methods:We have utilized a three-dimensional(3D)SG microenvironment composed of gelatin-alginate hydrogels and components from mouse SG extracellular matrix(SG-ECM)proteins to reroute the differentiation of MPCs to study the functions of this microenvironment.MPCs were encapsulated into the artificial SG microenvironment and were printed into a 3D cell-laden construct.The expression of specific markers at the protein and gene levels was detected after cultured 14 days.Results:Compared with the control group,immunofluorescence and gene expression assay demonstrated that MPCs encapsulated in the bioprinted 3D-SG microenvironment could significantly express the functional marker of mouse SG,sodium/potassium channel protein ATP1a1,and tend to express the specific marker of luminal epithelial cells,keratin-8.When the Shh pathway is inhibited,the expression of SG-associated proteins in MPCs under the same induction environment is significantly reduced.Conclusions:Our evidence proved the ability of differentiated mouse MPCs to regenerate SG cells by engineered SG microenvironment in vitro and Shh pathway was found to be correlated with the changes in the differentiation.These results provide insights into regeneration of damaged SG by MPCs and the role of the engineered microenvironment in reprogramming cell fate. 展开更多
关键词 3D bioprinting Artificial microenvironment DIFFERENTIATION Mammary progenitor cells sweat gland Extracellular matrix MPC ECM
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The interaction between epidermal growth factor (EGF) and matrix metalloproteinase induces the development of sweat glands in human fetal skin
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作者 Li Jianfu Fu Xiaobing Sheng Zhiyong 《感染.炎症.修复》 2001年第4期189-193,2,共6页
Objective:The development of sweat glands is a very complicated biological process involving many factors. In this study, we explore the inter-relationship between epidermal growth factor (EGF), matrix metalloproteina... Objective:The development of sweat glands is a very complicated biological process involving many factors. In this study, we explore the inter-relationship between epidermal growth factor (EGF), matrix metalloproteinases (MMP-2,MMP-7) and development of sweat glands in human embryos. Furthermore, we hope to elucidate the mechanism(s) underlying the induction of epidermal stem cells into sweat gland cells. 展开更多
关键词 Morphogenesis sweat gland Matrix metalloproteinase Epidermal growth factor
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Metabolic Regulation of Mammary Gland Epithelial Cells of Dairy Cow by Galactopoietic Compound Isolated from Vaccariae segetalis 被引量:10
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作者 TONG Hui-li GAO Xue-jun LI Qing-zhang LIU Jie LI Nan WAN Zhong-ying 《Agricultural Sciences in China》 CAS CSCD 2011年第7期1106-1116,共11页
In previous experiment, we isolated a compound dibutyl phthalate (DBP) from Vaccaria segetalis which had galactopoietic function on mammary gland epithelial cells of dairy cow (DCMECs). In this experiment, we asce... In previous experiment, we isolated a compound dibutyl phthalate (DBP) from Vaccaria segetalis which had galactopoietic function on mammary gland epithelial cells of dairy cow (DCMECs). In this experiment, we ascertained the metabolic regulation function of DBP on DCMECs. Many genes related to lactation including Stat5, AMPK, b-casein, Glut1, SREBP-1, PEPCK, and ACC were detected by real-time PCR. Furthermore, Stat5 and AMPK were detected by Western blot and immunofluorescence co-localization, respectively. The results showed that DBP stimulates the expression of Stat5 and p-Stat5, thus activates Stat5 cell signal transduction pathway and stimulates b-casein synthesis. DBP also raises the activities of Glut1 and AMPK to stimulate glucose uptake and glycometabolism and activates the expression of AMPK downstream target genes PEPCK and ACC and expression of SREBP-1 to stimulate milk fat synthesis. In addition, the activities of HK, G-6-PDH, ICDH, ATPase, and energy charges were stimulated by DBP to increase the energy metabolism level of DCMECs. The results showed DBP stimulates energy metabolism related to galactopoietic function in DCMECs. 展开更多
关键词 Vaccaria segetalis DBP dairy cow mammary gland epithelial cell metabolic regulation
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Small molecules facilitate single factor-mediated sweat gland cell reprogramming 被引量:4
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作者 Shuai-Fei Ji Lai-Xian Zhou +8 位作者 Zhi-Feng Sun Jiang-Bing Xiang Shao-Yuan Cui Yan Li Hua-Ting Chen Yi-Qiong Liu Huan-Huan Gao Xiao-Bing Fu Xiao-Yan Sun 《Military Medical Research》 SCIE CAS CSCD 2022年第6期655-667,共13页
Background: Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands(SGs), thus impairing the skin’s physiological function. This study aims to develop a stepwise reprog... Background: Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands(SGs), thus impairing the skin’s physiological function. This study aims to develop a stepwise reprogramming strategy to convert fibroblasts into SG lineages, which may provide a promising method to obtain desirable cell types for the functional repair and regeneration of damaged skin.Methods: The expression of the SG markers cytokeratin 5(CK5), cytokeratin 10(CK10), cytokeratin 18(CK18), carcinoembryonic antigen(CEA), aquaporin 5(AQP5) and α-smooth muscle actin(α-SMA) was assessed with quantitative PCR(qPCR), immunofluorescence and flow cytometry. Calcium activity analysis was conducted to test the function of induced SG-like cells(iSGCs). Mouse xenograft models were also used to evaluate the in vivo regeneration of iSGCs.BALB/c nude mice were randomly divided into normal group, SGM treatment group and iSGC transplantation group.Immunocytochemical analyses and starch-iodine sweat tests were used to confirm the in vivo regeneration of iSGCs.Results: Ectodermal dysplasia antigen(EDA) overexpression drove human dermal fibroblast(HDF) conversion into i SGCs in SG culture medium(SGM). qPCR indicated significantly increased mRNA levels of the SG markers CK5, CK18and CEA in iSGCs, and flow cytometry data demonstrated(4.18±0.04)% of iSGCs were CK5 positive and(4.36±0.25)%of iSGCs were CK18 positive. The addition of chemical cocktails greatly accelerated the SG fate program. qPCR results revealed significantly increased mRNA expression of CK5, CK18 and CEA in iSGCs, as well as activation of the duct marker CK10 and luminal functional marker AQP5. Flow cytometry indicated, after the treatment of chemical cocktails,(23.05±2.49)% of iSGCs expressed CK5^(+) and(55.79±3.18)% of iSGCs expressed CK18^(+), respectively. Calcium activity analysis indicated that the reactivity of iSGCs to acetylcholine was close to that of primary SG cells [(60.79±7.71)% vs.(70.59±0.34)%, ns]. In vivo transplantation experiments showed approximately(5.2±1.1)% of the mice were sweat test positive, and the histological analysis results indicated that regenerated SG structures were present in iSGCs-treated mice.Conclusions: We developed a SG reprogramming strategy to generate functional iSGCs from HDFs by using the single factor EDA in combination with SGM and small molecules. The generation of iSGCs has important implications for future in situ skin regeneration with SG restoration. 展开更多
关键词 Direct reprogramming Human dermal fibroblasts sweat gland REGENERATION
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Metastatic apocrine sweat gland adenocarcinoma in a terrier dog 被引量:1
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作者 Akhtardanesh Baharak Kheirandish Reza +3 位作者 Dabiri Shahriar Azari Omid Vosoogh Daruoosh Askari Nasrin 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2012年第8期670-672,共3页
This report describes the clinical and pathological aspects of an apocrine sweat gland carcinoma with distant metastasis in an aged dog.A 7-year-old male terrier dog was referred to small animal hospital of Shuhid Bah... This report describes the clinical and pathological aspects of an apocrine sweat gland carcinoma with distant metastasis in an aged dog.A 7-year-old male terrier dog was referred to small animal hospital of Shuhid Bahonar University of Kerman with a 5.5×3.5 centimeter pedunculated mass on its head near left auricular region which had been progressively growing since tliree months ago.The radiography showed no local and distant metastasis.Surgical excision and histological evaluation was done.Histologically,the mass was composed of epithelial cells arranged in glandular and solid patterns.The morphologic findings suggested either a primary or metastatic apocrine-gland carcinoma.Immunohistochemically,the tumor cells were intensely positive for cytokeratin 7 and 20 and negative for S100 protein.On the basis of histopathological and clinical findings,the tumor was diagnosed as a malignant apocrine gland tumor,arising from apocrine sweat glands of the skin.Local tumor recurrence with anorexia and weight loss was reported by the owner nine month later.Severe submandibular and prescapular lymphadenomegaly was noted in clinical examination.Several large pulmonary nodules were noted in chest radiographs resembling mediastinal lymph node metastasis.Second surgery and chemotherapy was rejected by the owner due to grave prognosis of the patient.The animal was died 45 days later due to respiratory complications.Tumors of apocrine sweat glands are relatively uncommon in dogs whereas apocrine gland adenocarcinoma with distant metastasis is extremely rare. 展开更多
关键词 APOCRINE sweat gland ADENOCARCINOMA DOG Metastasis Tumor recurrence Diagnosis
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Capacity of human umbilical cord-derived mesenchymal stem cells to differentiate into sweat gland-like cells:a preclinical study
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作者 Siming Yang Kui Ma +4 位作者 Changjiang Feng Yan Wu Yao Wang Sha Huang Xiaobing Fu 《Frontiers of Medicine》 SCIE CSCD 2013年第3期345-353,共9页
Human umbilical cord-derived mesenchymal stem cells(hUC-MSCs)possess various advantageous properties,including self-renewal,extended proliferation potential,multi-lineage differentiation potential and capacity for dif... Human umbilical cord-derived mesenchymal stem cells(hUC-MSCs)possess various advantageous properties,including self-renewal,extended proliferation potential,multi-lineage differentiation potential and capacity for differentiating into sweat gland-like cells in certain conditions.However,little is known about the effect of clinical-grade culture conditions on these properties and on the differentiative potential of hUC-MSCs.In this study,we sought to investigate the properties of hUC-MSCs expanded with animal serum free culture media(ASFCM)in order to determine their potential for differentiation into sweat gland-like cells.We found that primary cultures of hUC-MSCs could be established with ASFCM.Moreover,cells cultured in ASFCM showed vigorous proliferation comparable to those of cells grown in classical culture conditions containing fetal bovine serum(FBS).Morphology of hUC-MSCs cultured in ASFCM was comparable to those of cells grown under classical culture conditions,and hUC-MSCs grown in both of the two culture conditions tested showed the typical antigen profile of MSCs—positive for CD29,CD44,CD90,and CD105,and negative for CD34 and CD45,as expected.Chromosomal aberration assay revealed that the cells were stable after long-term culture under both culture conditions.Like normal cultured MSCs,hUC-MSCs induced under ASFCM conditions exhibited expression of the same markers(CEA,CK14 and CK19)and developmental genes(EDA and EDAR)that are characteristic of normal sweat gland cells.Taken together,our findings indicate that the classical culture medium used to differentiate hUC-MSCs into sweat gland-like cells can be replaced safely by ASFCM for clinical purposes. 展开更多
关键词 umbilical cord mesenchymal stem cells sweat gland PRECLINICAL
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Effect of Flos Buddlejae Granules on Apoptosis Factors Bax,Bcl-2,Fas and FasL in Lacrimal Gland Cells of Castrated Male Rabbits 被引量:2
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作者 Yun OU-YANG Jun PENG +3 位作者 Han-Yu TAN Wen-Juan LI Quan-Long WU Qing-Hua PENG 《Digital Chinese Medicine》 2018年第2期115-121,共7页
Objective To observe the effect of different concentration Flos Buddlejae(Mi Meng Hua,密蒙花)granules on apoptosis factors of lacrimal gland cells of castrated male rabbits,and study the treatment effects of Flos Budd... Objective To observe the effect of different concentration Flos Buddlejae(Mi Meng Hua,密蒙花)granules on apoptosis factors of lacrimal gland cells of castrated male rabbits,and study the treatment effects of Flos Buddlejae(Mi Meng Hua,密蒙花)granules in the dry eye model of castrated male rabbits.Methods Flos Buddlejae(Mi Meng Hua,密蒙花)raw material was made into granules.Thirty healthy adult New Zealand rabbits were randomly divided into five groups,six rabbits in each group.Group A:blank group,Group B:model group,Group C:Flos Buddlejae(Mi Meng Hua,密蒙花)granule group,Group D:placebo group,Group E:testosterone group.Except for group A,all rabbits underwent removal of bilateral testis and epididymis.Rabbits in group C were administered Flos Buddlejae(Mi Meng Hua,密蒙花)granules(100 mg/kg),three times per day;rabbits in group D were administered normal saline,three times per day.Rabbits in group E were injected with testosterone propionate(0.5 mL/kg)in the thigh muscle,every 3 days.All rabbits were tested by Schirmer I test(SIT)and tear film break-up time(BUT)before operation and 4 weeks after operation.After 4 weeks,all rabbits were sacrificed by air embolism and clipping of the lacrimal gland.Apoptosis factors,including Bax,Bcl-2,Fas and FasL of lacrimal gland cells were characterized by immunohistochemistry,and resulting data were statistically analyzed.Results(1)Comparison of SIT and BUT before and after operation:There were statistically significant differences between groups B and D(P<0.01),but not among other groups(P>0.05).(2)Comparison of apoptosis factors Bax,Bcl-2 Fas and FasL:In a comparison of groups B and D,there was no statistically significant difference after operation(P>0.05).In a comparison of the other groups,there were statistically significant differences(P<0.01).In comparisons among A,C and E groups,there were no statistically significant differences(P>0.05).Conclusion Compared with androgen,Flos Buddlejae(Mi Meng Hua,密蒙花)granules caused similar but slightly weaker depression of Bax,Fas and FasL,and increased expression of Bcl-2. 展开更多
关键词 Flos Buddlejae Granules Dry eye Castrated male rabbits Lacrimal gland cell Apoptosis factors
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Malignant sweat gland tumor of breast arising in pre-existing benign tumor: A case report
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作者 Jin Kyung An Jeong Joo Woo Young Ok Hong 《World Journal of Clinical Cases》 SCIE 2019年第19期3033-3038,共6页
BACKGROUND Sweat glands belong to skin appendages.Sweat gland tumors are uncommon,especially when they occur as malignant tumors in the breast.We report a case of malignant sweat gland tumor of the breast,including im... BACKGROUND Sweat glands belong to skin appendages.Sweat gland tumors are uncommon,especially when they occur as malignant tumors in the breast.We report a case of malignant sweat gland tumor of the breast,including imaging and pathological findings.CASE SUMMARY A 47-year-old woman visited our hospital with a non-tender palpable lesion in her left breast.The lesion had not shown changes for 10 years.However,it recently increased in size.Sonography showed a well circumscribed cystic lesion with internal debris and fluid-fluid level.Magnetic resonance imaging showed a well circumscribed oval mass with T1 hyper-intensity compared to muscle and T2 high signal intensity.There was a small enhancing mural component in the inner wall of the mass.The tumor was resected.Its pathologic result was a malignant transformation of benign sweat gland tumor such as hidradenoma.The lesion was treated with excision and radiation therapy.At 1-year follow up,there was no local recurrence or metastasis in the patient.CONCLUSION In the case of a rapid growing cystic mass in the nipple and subareola,it is necessary to distinguish it from a malignant sweat gland tumor. 展开更多
关键词 sweat gland NEOPLASM BREAST ULTRASONOGRAPHY Magnetic resonance imaging
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Construction of Antibacterial Peptide CecropinB Eukaryotic Recombinant Vector and Its Expression in Dairy Goat Mammary Gland Epithelial Cells
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作者 GAO Xuejun TONG Huili YIN Deyun ZHANG Li 《Journal of Northeast Agricultural University(English Edition)》 CAS 2008年第4期15-19,共5页
To investigate the expression of antibacterial peptide CecropinB cDNA in dairy goat mammary gland epithelial cells,the CecropinB gene was cloned and was inserted into a eukaryotic vector pECFP-C1 to construct the reco... To investigate the expression of antibacterial peptide CecropinB cDNA in dairy goat mammary gland epithelial cells,the CecropinB gene was cloned and was inserted into a eukaryotic vector pECFP-C1 to construct the recombinant plasmid pECFP-B by genetic engineering technique.Recombinant plasmid pECFP-B was transfected into dairy goat mammary gland epithelial to detect the bactericidal activity of CecropinB.The expression of CecropinB was also detected.The result of RT-PCR demonstrated CecropinB gene was expressed in transfected cells.CecropinB recombinant plasmid DNA was injected into udders and CecropinB was expressed in mammary gland,exhibiting bactericidal activity to Staphylococcus aureus in vivo experiments. 展开更多
关键词 Cecropin B goat mammary epithelial gland cell TRANSFECTION bactericidal activity
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