Three bacterial strains Bacillus pumilis, Cellulosimicrobium cellulans and ExtguoOactertum were investigated when grown in Luria-Bertani （LB） medium at 500 μg/mL Cr（VI）. The hexavalent chromium reduction was meas...Three bacterial strains Bacillus pumilis, Cellulosimicrobium cellulans and ExtguoOactertum were investigated when grown in Luria-Bertani （LB） medium at 500 μg/mL Cr（VI）. The hexavalent chromium reduction was measured by growing the strains in DeLeo and Ehrlich （1994） medium at 200 and 400 μg/mL K2CrO4. The optimal Cr （VI） reduction by strains B. pumilis, Exigubacterium and C. cellulans was 51%, 39%, and 41%, respectively, at an initial KzCrO4 concentration of 200 ~tg/mL at pH 3 and temperature 37℃. At an initial chromate concentration of 400 gg/mL, the Cr（VI） reduction by strains B. purnilis, Exigubacterium and C. cellulans was 24%, 19%, and 18%, respectively at pH 3 at 37℃ after 24 h. These strains have ability to reduce toxic hexavalent chromium to the less mobile trivalent chromium at a wide range of different environmental conditions and can be useful for the treatment of contaminated wastewater and soils.展开更多
在纤维素刚果红培养基中筛选具有透明圈的方法,从肥沃的土壤中筛选获得一株溶解圈直径与菌落直径比值(D/d)可达4.75的细菌,命名为DBJ。该菌最适产纤维素酶的培养时间是60 h,培养温度是35℃,起始p H 7.0。通过形态学、生理生化试验和16S ...在纤维素刚果红培养基中筛选具有透明圈的方法,从肥沃的土壤中筛选获得一株溶解圈直径与菌落直径比值(D/d)可达4.75的细菌,命名为DBJ。该菌最适产纤维素酶的培养时间是60 h,培养温度是35℃,起始p H 7.0。通过形态学、生理生化试验和16S r DNA序列比对分析对DBJ进行了鉴定。结果表明,革兰氏阳性菌DBJ为芬氏纤维微菌(Cellulosimicrobium funkei),将其命名为Cellulosimicrobium funkei DBJ,DBJ的16S r DNA序列的Gen Bank登录号为JX392404。展开更多
文摘Three bacterial strains Bacillus pumilis, Cellulosimicrobium cellulans and ExtguoOactertum were investigated when grown in Luria-Bertani （LB） medium at 500 μg/mL Cr（VI）. The hexavalent chromium reduction was measured by growing the strains in DeLeo and Ehrlich （1994） medium at 200 and 400 μg/mL K2CrO4. The optimal Cr （VI） reduction by strains B. pumilis, Exigubacterium and C. cellulans was 51%, 39%, and 41%, respectively, at an initial KzCrO4 concentration of 200 ~tg/mL at pH 3 and temperature 37℃. At an initial chromate concentration of 400 gg/mL, the Cr（VI） reduction by strains B. purnilis, Exigubacterium and C. cellulans was 24%, 19%, and 18%, respectively at pH 3 at 37℃ after 24 h. These strains have ability to reduce toxic hexavalent chromium to the less mobile trivalent chromium at a wide range of different environmental conditions and can be useful for the treatment of contaminated wastewater and soils.
文摘在纤维素刚果红培养基中筛选具有透明圈的方法,从肥沃的土壤中筛选获得一株溶解圈直径与菌落直径比值(D/d)可达4.75的细菌,命名为DBJ。该菌最适产纤维素酶的培养时间是60 h,培养温度是35℃,起始p H 7.0。通过形态学、生理生化试验和16S r DNA序列比对分析对DBJ进行了鉴定。结果表明,革兰氏阳性菌DBJ为芬氏纤维微菌(Cellulosimicrobium funkei),将其命名为Cellulosimicrobium funkei DBJ,DBJ的16S r DNA序列的Gen Bank登录号为JX392404。
文摘为实现对黄曲霉毒素M1(Aflatoxin M1,AFM1)的生物降解,建立从环境中筛选AFM1降解菌株的方法并得到降解菌株。结果表明:对采自农田、粮库、牛场的300个土壤或粪便样品,初筛选出能在以香豆素为唯一碳源的培养基上生长的菌株37株;用液相色谱法测定降解率进行复筛,得到3株降解率在50%以上的菌株;进而对复筛菌株进行生长条件和耐受性实验,筛选出降解率为78.6%,生长速度快、长势好、稳定期长,且降解过程中热稳定性好的菌株102807。经过菌落细胞形态学、生理生化特征及16S r DNA序列系统发育分析,鉴定为纤维纤维微菌(Cellulosimicrobium cellulans)。首次发现了纤维纤维微菌对AFM1的生物降解功能。
