[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm...[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm,5 μm),mobile phase of acetonitrile-0. 2% phosphoric acid aqueous solution gradient elution,flow rate of 0. 7 m L/min,column temperature of 30 ℃,detection wavelength of 320nm; the chromatographic fingerprint similarity evaluation software( Version 2004 A) was used for similarity evaluation and data processing on 10 origin Thlaspi arvense L. spectra,and the median method was used to generate control Thlaspi arvense L. fingerprint. [Results] The similarity of 10 origin Thlaspi arvense L. spectra was greater than 0. 90,19 common peaks were separated from different origin Thlaspi arvense L. and isovitexin,apigenin,luteolin and acacetin were identified. [Conclusions] The established HPLC fingerprint contained a lot of information and showed good specificity for Thlaspi arvense L.,which can provide a scientific basis for Thlaspi arvense L. quality evaluation system.展开更多
Horsetail (Equisetum arvense L.) is a perennial herb which creates during the life cycle spring and summer stems. The selected species and populations were monitored in the years 2009-2011 in three different natural l...Horsetail (Equisetum arvense L.) is a perennial herb which creates during the life cycle spring and summer stems. The selected species and populations were monitored in the years 2009-2011 in three different natural locations in Laborecká vrchovina (Slovakia). Samples were collected by destructive methods in all three locations. Silicon content was determined in dry biomass by AAS. Silicon content in plants ranged from 21.11 ± 3.24 g·kg-1 to 32.80 ± 8.03 g·kg-1. The highest content of silicon exhibited samples of the September collection. We found that the location and the year in terms of silicon content were not statistically significant. The main sources for statistical variability in the accumulation of silicon were during the collections.展开更多
Objective:The chemical components of the essential oil from Thlaspi arvense L.were firstly studied.Methods:The essential oil was extracted by steam distillation and analyzed by GC/MS technique.Results:32 compositions ...Objective:The chemical components of the essential oil from Thlaspi arvense L.were firstly studied.Methods:The essential oil was extracted by steam distillation and analyzed by GC/MS technique.Results:32 compositions were isolated and identified representing 99.38% of the total contents of the essential.Conclusion:The essential oil from Thlaspi arvense L.mainly consists of allyl sulfide.展开更多
文摘[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm,5 μm),mobile phase of acetonitrile-0. 2% phosphoric acid aqueous solution gradient elution,flow rate of 0. 7 m L/min,column temperature of 30 ℃,detection wavelength of 320nm; the chromatographic fingerprint similarity evaluation software( Version 2004 A) was used for similarity evaluation and data processing on 10 origin Thlaspi arvense L. spectra,and the median method was used to generate control Thlaspi arvense L. fingerprint. [Results] The similarity of 10 origin Thlaspi arvense L. spectra was greater than 0. 90,19 common peaks were separated from different origin Thlaspi arvense L. and isovitexin,apigenin,luteolin and acacetin were identified. [Conclusions] The established HPLC fingerprint contained a lot of information and showed good specificity for Thlaspi arvense L.,which can provide a scientific basis for Thlaspi arvense L. quality evaluation system.
基金The work was supported by the Agency of Ministry of Education,Science,Research and Sport of the Slovak Republic,the project:00162-0001(MS SR-3634/2010-11).
文摘Horsetail (Equisetum arvense L.) is a perennial herb which creates during the life cycle spring and summer stems. The selected species and populations were monitored in the years 2009-2011 in three different natural locations in Laborecká vrchovina (Slovakia). Samples were collected by destructive methods in all three locations. Silicon content was determined in dry biomass by AAS. Silicon content in plants ranged from 21.11 ± 3.24 g·kg-1 to 32.80 ± 8.03 g·kg-1. The highest content of silicon exhibited samples of the September collection. We found that the location and the year in terms of silicon content were not statistically significant. The main sources for statistical variability in the accumulation of silicon were during the collections.
文摘Objective:The chemical components of the essential oil from Thlaspi arvense L.were firstly studied.Methods:The essential oil was extracted by steam distillation and analyzed by GC/MS technique.Results:32 compositions were isolated and identified representing 99.38% of the total contents of the essential.Conclusion:The essential oil from Thlaspi arvense L.mainly consists of allyl sulfide.
