The difference of composition in extracellular fluid in brain parenchyma and CSF suggests that CSF-brain barrier(CBB)is objective,which is necessary to maintain the stability of their internal environment.However,the ...The difference of composition in extracellular fluid in brain parenchyma and CSF suggests that CSF-brain barrier(CBB)is objective,which is necessary to maintain the stability of their internal environment.However,the information exchange between brain and CSF is also objective.For example,CSF is changes during diseases,the application of drugs(such as lumbar anesthesia)or tissue transplantation via the CSF pathway may also produce central(cerebral)effects.Who breaks through the barrier and mediates brain-CSF communication?Since 1992,we have found that the peripheral nerve tracer CB-HRP injected into the lateral ventricle of mice,rats and macaques is blocked in the ventricle wall and can only run in CSF,but a large number of CB-HRP-positive labeled neurons can be seen in specific parts of the brain stem parenchyma.Understandably,these neurons touch markers in CSF.Otherwise,they cannot be tagged.展开更多
Objective: To probe into the significance of Nitric Oxide (NO) in ischemic cerebral damage and effect of ligustrazine on it. Methods: The NO contents in cerebrospinal fluid (CSF) and plasma of 20 controls and 62 patie...Objective: To probe into the significance of Nitric Oxide (NO) in ischemic cerebral damage and effect of ligustrazine on it. Methods: The NO contents in cerebrospinal fluid (CSF) and plasma of 20 controls and 62 patients with arteriosclerotic thrombotic cerebral infarction (ligustrazine group and common treatment group) were determined with Griess method before and after treatment. Results:The NO content in CSF before treatment was higher in severe type, it was higher than that in moderate type, and than that in mild type,again higher than that in control group (all P < 0. 05) and was positively correlated with the size of infarction (P < 0. 01 ). There was no significant difference between the plasma NO content of patients and that of control group (P < 0. 05), but there was a trend that plasma NO content decreased as the patient's condition worsened. After treatment, the curative effect and plasma NO content of ligustrazine group were both markedly higher than those of common treatment group (P < 0. 05, 0. 05 ).There were no significant differences between CSF NO contents of the two groups, respectively, and that of control group (P > 0. 05,0. 05). Conclusion: Excessive NO produced in brain tissue and insufficient plasma NO participate the course of ischemic brain damage.Ligustrazine could increase the contents of plasma NO selectively.Original article on CJIM(Chin) 1998; 18(6). 342展开更多
Drugs that lack the ability to cross the blood- brain barrier （BBB） need to be placed directly into the central nervous system. Our laboratory studies the involve- ment of the glutamatergic system in the aggressiven...Drugs that lack the ability to cross the blood- brain barrier （BBB） need to be placed directly into the central nervous system. Our laboratory studies the involve- ment of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebel- lomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solu- tion. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.展开更多
文摘The difference of composition in extracellular fluid in brain parenchyma and CSF suggests that CSF-brain barrier(CBB)is objective,which is necessary to maintain the stability of their internal environment.However,the information exchange between brain and CSF is also objective.For example,CSF is changes during diseases,the application of drugs(such as lumbar anesthesia)or tissue transplantation via the CSF pathway may also produce central(cerebral)effects.Who breaks through the barrier and mediates brain-CSF communication?Since 1992,we have found that the peripheral nerve tracer CB-HRP injected into the lateral ventricle of mice,rats and macaques is blocked in the ventricle wall and can only run in CSF,but a large number of CB-HRP-positive labeled neurons can be seen in specific parts of the brain stem parenchyma.Understandably,these neurons touch markers in CSF.Otherwise,they cannot be tagged.
文摘Objective: To probe into the significance of Nitric Oxide (NO) in ischemic cerebral damage and effect of ligustrazine on it. Methods: The NO contents in cerebrospinal fluid (CSF) and plasma of 20 controls and 62 patients with arteriosclerotic thrombotic cerebral infarction (ligustrazine group and common treatment group) were determined with Griess method before and after treatment. Results:The NO content in CSF before treatment was higher in severe type, it was higher than that in moderate type, and than that in mild type,again higher than that in control group (all P < 0. 05) and was positively correlated with the size of infarction (P < 0. 01 ). There was no significant difference between the plasma NO content of patients and that of control group (P < 0. 05), but there was a trend that plasma NO content decreased as the patient's condition worsened. After treatment, the curative effect and plasma NO content of ligustrazine group were both markedly higher than those of common treatment group (P < 0. 05, 0. 05 ).There were no significant differences between CSF NO contents of the two groups, respectively, and that of control group (P > 0. 05,0. 05). Conclusion: Excessive NO produced in brain tissue and insufficient plasma NO participate the course of ischemic brain damage.Ligustrazine could increase the contents of plasma NO selectively.Original article on CJIM(Chin) 1998; 18(6). 342
基金supported by Coordenacao de Aperfeicoamento de Pessoal de Nível Superior(CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)-Edital Doencas Neurodegenerativas+1 种基金Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul(FAPERGS)Financiadora de Estados e Projetos(FINEP)
文摘Drugs that lack the ability to cross the blood- brain barrier （BBB） need to be placed directly into the central nervous system. Our laboratory studies the involve- ment of the glutamatergic system in the aggressiveness of glioma, and some ligands of glutamate receptors cannot permeate the BBB. Here, glioma-implanted rats were treated by a technique that delivers ligands directly into the cerebrospinal fluid by puncture into the cisterna cerebel- lomedullaris. Rats were anesthetized and fixed in a rodent stereotactic device. The head was gently tilted downwards at an angle that allowed exposure of the cisterna. Injection into the cisterna was done freehand using a gingival needle coupled to a microsyringe. The efficiency of intracisternal injection was demonstrated using a methylene blue solu- tion. This type of injection is adaptable for any rodent model using small volumes of a variety of other drugs, and is an interesting method for neuroscience studies.
