Hollow cerium nanoparticles synthesized by one-step method for multienzyme activity to reduce colitis in mice

BACKGROUND Inflammatory bowel disease(IBD)is a common chronic intestinal inflammatory disease.High oxidative stress is a treatment target for IBD.Cerium oxide(CeO2)nanomaterials as nanozymes with antioxidant activity are potential drugs for the treatment of colitis.AIM To synthesize hollow cerium(H-CeO2)nanoparticles by one-step method and to validate the therapeutic efficacy of H-CeO2 in IBD.METHODS H-CeO2 was synthesized by one-step method and examined its characterization and nanoenzymatic activity.Subsequently,we constructed dextran sulfate so-dium(DSS)-induced colitis in mice to observe the effects of H-CeO2 on colonic inflammation.The effects of H-CeO2 on colon inflammation and reactive oxygen species(ROS)levels in IBD mice were detected by hematoxylin and eosin staining and dichlorofluorescein diacetate staining,respectively.Finally,the biological sa-fety of H-CeO2 on mice was evaluated by hematoxylin and eosin staining,blood routine,and blood biochemistry.RESULTS H-CeO2 nanoparticles prepared by the one-step method were uniform,monodi-sperse and hollow.H-CeO2 had a good ability to scavenge ROS,∙OH and∙OOH.H-CeO2 reduced DSS-induced decreases in body weight and colon length,colonic epithelial damage,inflammatory infiltration,and ROS accumulation.H-CeO2 administration reduced the disease activity index of DSS-induced animals from about 8 to 5.H-CeO2 had no significant effect on body weight,total platelet count,hemoglobin,white blood cell,and red blood cell counts in healthy mice.No significant damage to major organs was observed in healthy mice following H-CeO2 administration.CONCLUSION The one-step synthesis of H-CeO2 nanomaterials had good antioxidant activity,biosafety,and inhibited deve-lopment of DSS-induced IBD in mice by scavenging ROS.

Improved super-elastic Ti-Ni alloy wire for the angle class III patient with anterior open bite:A case report

BACKGROUND Orthodontic treatment for open bite and crossbite cases is always challenging.In this paper,we demonstrate a skeletal class III patient with anterior open bite and crossbite whose problem was successfully corrected with improved super-elastic Ti-Ni alloy wire(ISW).CASE SUMMARY A 19 years old male came to our clinic with a chief complaint of anterior open bite and crossbite and not able to chew food well.Clinical examination revealed an angle class III malocclusion with anterior open bite,crossbite and spaced arch.Ra-diographic and clinical examination showed a skeletal class III pattern.We used ISW to level the upper and lower arch and to correct the anterior open bite and crossbite.Intermaxillary elastics were also used to achieve a better interdigitation.Finally,adequate overbite,overjet and a desirable occlusion were achieved.The active treatment time took 2 years and 2 months.CONCLUSION In a case of class III angular malocclusion with open bite and crossbite in the ante-rior teeth,ideal results were achieved using the ISW technique and the patient was satisfied with the outcome.

共表达分子伴侣蛋白提高III型普鲁兰水解酶在短小芽孢杆菌中的分泌表达及发酵优化

本研究旨在通过共表达分子伴侣蛋白以及优化发酵条件来提高III型普鲁兰水解酶(TK-PUL)在短小芽孢杆菌中的分泌表达水平。通过构建共表达TK-PUL和分子伴侣蛋白的多种重组短小芽孢杆菌,并以胞外酶活为指标对其进行筛选,确定最有利于TK-PUL分泌表达的分子伴侣蛋白及对应的重组短小芽孢杆菌。在此基础上采用单因素实验和响应面法优化重组短小芽孢杆菌的发酵条件。结果表明,共表达分子伴侣蛋白PrsA^(Ba)的重组短小芽孢杆菌的胞外酶活达到98.79 U/mL,提高了0.31倍。该重组短小芽孢杆菌的最佳培养基包括19.65 g/L葡萄糖、21.46 g/L酵母提取物、12.01 g/L MgCl_(2)·6H_(2)O、9.02 g/L脯氨酸、0.01 g/L FeSO4·7H_(2)O、0.01 g/L MnSO4·4H_(2)O、0.001 g/L ZnSO4·7H_(2)O。在发酵温度为35℃、起始发酵pH为7.0的条件下,该重组短小芽孢杆菌于最佳培养基中培养66 h时,其胞外酶活达到192.68 U/mL,提高了1.56倍。本研究通过共表达分子伴侣蛋白和发酵优化实现了TK-PUL在短小芽孢杆菌的高效分泌表达,为TK-PUL的应用提供了基础。

基于NSGA-III算法求解柔性作业车间调度问题

针对多目标柔性作业车间调度问题,提出一种改进NSGA-Ⅲ算法,以完工时间、机器总负荷、瓶颈机器负荷为目标建立调度模型。首先,为提高种群的多样性,提出一种基于惩罚的边界相交距离定义关联操作中种群个体与参考向量之间的距离;其次,为提高环境选择的计算效率,利用基于惩罚的边界相交距离消除机制来保护个体,降低了个体保护策略的计算成本;最后,为避免种群陷入局部最优,在遗传算子中采用一种改进的变异策略。运用两个评价指标与NSGA-Ⅲ算法进行比较,其收敛性与多样性均由于NSGA-Ⅲ算法。同时在4个Kacem算例上进行测试得出改进NSGA-Ⅲ算法解的质量较高,最后通过实际的生产实例证明改进的NSGA-Ⅲ算法优于或等同于现存在的方法,也证明了该方法在解决多目标柔性作业车间调度难题上的可操作性。

混合牙列期骨性III类错合与骨性I类错合基骨及牙弓对比研究

目的:通过测量混合牙列期骨性III类错合患者与骨性I类错合患者的锥形束计算机断层扫描(Cone beam computed tomography, CBCT),对比分析上下颌牙弓、基骨弓长度与宽度差异及协调性,以期为替牙期骨性III类错合畸形治疗提供参考依据。方法:选择替牙列期骨性III类错合与骨性I类错合各30例,通过Dolphin软件处理收集到的DICOM (Digital Imaging and Communications in Medicine)医学图像数据,行三维重建并测量上下颌牙弓、基骨弓的长度与宽度,计算同一牙段牙弓宽度与基骨弓宽度之间的差值,比较骨性III类与骨性I类错牙合畸形牙弓与基骨弓宽度差值的差异。结果:1) 骨性III类错合组上颌牙弓及基骨弓长度均小于骨性I类错合组,下颌基骨弓长度大于骨性I类错合组,且差异有统计学意义(P 0.05)。2) 骨性III类错合组下颌前段及中段基骨弓宽度大于骨性I类错合组,差异有统计学意义(P 0.05)。3) 骨性III类错合组上颌牙弓与基骨弓宽度的差值均大于骨性I类错合组,下颌牙弓与基骨弓宽度的差值均小于骨性I类错合组,其中上颌后段牙弓宽度与基骨弓宽度差值差异有统计学意义(P < 0.05)。结论:混合牙列期骨性III类错合存在牙弓、基骨弓不调,上颌可扩大基骨弓,下颌可扩大牙弓,以期改善牙弓与基骨弓的协调性。

Synthesis and Structure of One Novel Polyoxometalate Compound Connected via Cerium(III) Cation with Three-dimensional Framework H_2{[K(H_2O)_2]_2[Ce(H_2O)_5]_2(H_2Mo_(1.16)W_(10.84)O_(42))}·8H_2O

One novel polyoxometalate compound connected via trivalent cerium cation as bridge H2{ [K（H2O）212[Ce（H2O）5]2（H2Mo1.16W10.84O42）}.8H2O 1 was designed and synthesized in aqueous solution. X-ray diffraction analysis reveals that the structure of 1 is a three-dimensional framework assembled from the arrangement of H2Mo1.16W10.84O42 （named paradodecmetalate-B） and Ce（H2O）5^3＋ containing two planes, which are constructed through the unification of H2Mo1.16W10.84O42^10- and Ce（H2O）5^3＋ along the [100] and [001] directions. Crystal data： H96Ce4KaMo2.32O128W21.68, Mr = 7074.89, monoclinic, P21/n, a = 12.5037（17）, b = 17.002（2）, c = 12.7473（17）A, β = 105.966（2）°, V = 2605.4（6）A^3, Z = 1, Dc = 4.509 g/cm^3, F（000） = 3132,μ = 26.098 mm^-1, R = 0.0377 and wR = 0.0789 （I 〉 2σ（I））.

基于改进NSGA-III算法的多无人机协同目标分配

武器-目标分配问题是战场环境下无人机对敌方执行打击任务的关键,其目的是基于目标的威胁、价值和我方武器的毁伤概率,寻找合理的武器目标分配方案,以提高作战效率。针对当前多目标优化算法解决静态武器-目标分配问题时收敛速度慢、收敛稳定性差,难以适应当前战场高度实时性的问题,提出一种改进的基于参考点的非支配排序遗传算法。通过二进制编码打击方案并优化初始种群,引入自适应变异与交叉策略以及种群寻优更新策略,基于对战场态势进行评估得到的威胁矩阵和优势矩阵,种群多次迭代后生成目标打击方案。最后计算满足约束条件的Pareto解集,并将Pareto前沿中的相对最优解作为多无人机的打击方案。多次实验证明,在较好情况下改进算法相比于原始算法的收敛时间减少46.74%,目标威胁值降低50.5%,总飞行航程减少26.46%,杀伤目标数增加11.76%,证明该算法在解决多无人机空对地打击任务目标分配问题时具有合理性和高效性。

III型胶原蛋白在乳腺癌中的研究进展

乳腺癌(Breast cancer, BC)于2020年在全球新增癌症病例成为了最常见癌症,且发病率逐年升高。胶原蛋白是构成细胞外基质(Extracellular Matrix, ECM)的主要成分,近年来的研究发现III型胶原蛋白(Collagen Type III, COLIII)在乳腺癌的发生、发展过程中发挥重要作用,并对肿瘤微环境(Tumor micro-environment, TME)的维持有重要意义。其中,癌症相关成纤维细胞(Cancer-associated fibroblasts, CAFs)在建立和重塑ECM结构中扮演关键角色,调控肿瘤细胞通过TME入侵的进程。但由于该体系庞大,通过分泌细胞因子和趋化因子与癌细胞相互作用的机制复杂,各成分及其亚型在癌症进展中的角色尚存争议。本文基于TME在乳腺恶性肿瘤中的研究现状以及作为ECM中重要成分的COLIII的性质结构、形态分布,分析COLIII通过改变ECM的机械力与刚度影响乳腺癌行为的机制,同时充分讨论特殊结构、来源的COLIII在微环境中的不同功能及作用方式,评估其应用于癌症治疗的可能性。

Observation of Curative Effect of Surgical Nursing in Patients with Grade III Hand-Foot Syndrome

Objective: To investigate the effect of surgical nursing on grade III Hand-Foot Syndrome (HFS) induced by Doxorubicin hydrochloride liposome chemotherapy after breast cancer surgery. Method: From January 2019 to December 2019, 10 patients with HFS grade III caused by Doxorubicin hydrochloride liposome chemotherapy after breast cancer surgery in the Breast Department of Cancer Prevention and Treatment Center of Sun Yat-sen University were selected, and surgical nursing methods were used to intervene and observe the therapeutic effects of the patients. Results: One patient was cured within 7 days, the cure rate was 10% in 7 days, 8 patients were cured within 10 days, the cure rate was 80% in 10 days, 10 patients were cured within 15 days, the cure rate was 100% in 15 days. Conclusion: The surgical nursing method is effective for patients with grade III HFS caused by Doxorubicin hydrochloride liposome chemotherapy after breast cancer surgery, shortening the treatment time of HFS, and is worthy of clinical promotion.

Phase composition,conductivity,and sensor properties of cerium-doped indium oxide

The hydrothermal synthesis of In_(2)O_(3)and CeO_(2)–In_(2)O_(3)is investigated as well as the properties of sensor layers based on these compounds.During the synthesis of In_(2)O_(3),intermediate products In(OH)_(3)and InOOH are formed,which are the precursors of stable cubic(c-In_(2)O_(3))and metastable rhombohedral(rh-In_(2)O_(3))phases,respectively.A transition from c-In_(2)O_(3)to rh-In_(2)O_(3)is observed with the addition of CeO_(2).The introduction of cerium into rh-In_(2)O_(3)results in a decrease in the sensor response to hydrogen,while it increases in composites based on c-In_(2)O_(3).The data on the sensor activity of the composites correlate with XPS results in which CeO_(2)causes a decrease in the concentrations of chemisorbed oxygen and oxygen vacancies in rh-In_(2)O_(3).The reverse situation is observed in composites based on c-In_(2)O_(3).Compared to In_(2)O_(3)and CeO_(2)–In_(2)O_(3)obtained by other methods,the synthesized composites demonstrate maximum response to H_(2)at low temperatures by 70–100℃,and have short response time(0.2–0.5 s),short recovery time(6–7 s),and long-term stability.A model is proposed for the dependence of sensitivity on the direction of electron transfer between In_(2)O_(3)and CeO_(2).

Value of negative pressure sealing drainage on wound healing of Gustilo IIIB and IIIC open fractures

BACKGROUND Gustilo III fractures have a high incidence and are difficult to treat.Patients often experience difficulty in wound healing.Negative pressure drainage technology can help shorten wound healing time and has positive value in improving patient prognosis.AIM To explore the clinical value of the negative pressure sealing drainage technique in wound healing of Gustilo IIIB and IIIC open fractures.METHODS Eighty patients with Gustilo IIIB and IIIC open fractures with skin and soft tissue injuries who were treated in the Second People’s Hospital of Dalian from March 2019 to December 2021 were selected as the research subjects.They were divided into a study group(n=40,healed with negative pressure closed drainage)and a control group(n=40,healed with conventional dressing changes)according to the variation in the healing they received.The efficacy of the clinical interventions,the variations in the regression indicators(time to wound healing,time to fracture healing,time to hospitalization),and the conversion and healing of bacterial wounds were compared 1-3 mo after the intervention.RESULTS The total effective rate of patients among the study group was 95.00%(38/40),which was notably higher than 75.00%(30/40)among the control group(P<0.05).The wound healing time,fracture healing time,and hospital stay of the patients in the study group was shorter than the control group(P<0.05).After the intervention,the negative bacterial culture at the wound site rate and wound healing rate of the patients among the study group increased compared to the control group(P<0.05).CONCLUSION Negative pressure sealing and drainage technology has a good therapeutic effect on patients with Gustilo IIIB and IIIC open fracture wounds with skin and soft tissue injury.It can notably enhance the wound healing rate and the negative rate of bacteria on the wound surface and help to speed up the recovery process of patients.

Target-induced Trivalent G-quadruplex/hemin DNAzyme for Colorimetric Detection of Hg^(2+) Based on an Exonuclease III Assisted Catalytic Hairpin Assembly

Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg^(2+).A hairpin DNA(Hr)was designed with thymine-Hg^(2+)-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg^(2+).A released DNA fragment triggers the catalytic assembly of other three hairpins(H1,H2,and H3)to form many trivalent G-quadruplex/hemin DNA enzymes for signal output.The developed sensor shows a dynamic range from 2 pM to 2μM,with an impressively low detection limit of 0.32 pM for Hg^(2+)detection.Such a sensor also has good selectivity toward Hg^(2+)detection in the presence of other common metal ions.This strategy shows the great potential for visual detection with portable type.

Geobacter-associated prophages confer beneficial effect on dissimilatory reduction of Fe(III)oxides

The dissimilatory reduction of Fe(III)oxides driven by Fe(III)-reducing bacteria(FRB)is an important biogeo-chemical process that influences not only iron cycling but also the biogeochemical cycles of carbon,trace metals,nutrients and contaminants.Phages have central roles in modulating the population and activity of FRB,but the mechanism for phage-involved Fe(III)oxide reduction is still unclear.This work used a common FRB,Geobacter soli,to explore the roles and underlying mechanisms of FRB-harboring prophages in the dissimilatory reduction of Fe(III)oxides.Bioinformatic analysis predicted 185 phage-related genes in the G.soli genome,comprising functional prophages that were verified to be induced to form tailed phage particles.Ferrihydrite reduction was facilitated as prophage induction was stimulated and declined as prophage induction was inhibited,which indi-cated a positive role of G.soli-harboring prophages in Fe(III)oxide reduction.A comparison of gene expression and released phage particles in the cells grown with Fe(III)-citrate and ferrihydrite suggested that microbial fer-rihydrite reduction would activate the SOS response and consequently induce the prophages to enter lytic cycles.The prophage-mediated lysis of the subpopulation resulted in an increased release of extracellular DNA and mem-brane vesicles that were conducive to Fe(III)oxide reduction,which might explain the positive role of prophages in ferrihydrite reduction.In summary,our results revealed the functional roles and underlying mechanisms of FRB-associated prophages in facilitating the dissimilatory reduction of Fe(III)oxides,which will advance our understanding of iron cycling in natural ecosystems.

中青年视网膜静脉阻塞与同型半胱氨酸、抗凝血酶III相关研究

目的:研究分析中青年视网膜静脉阻塞(Retinal Vein Occlusion, RVO)患者同型半胱氨酸(Homocysteine, Hcy)及抗凝血酶III (Antithrombin III, AT-III)水平,两者是否作为致病危险因素参与RVO发病。分析视网膜中央静脉阻塞(CRVO)和视网膜分支静脉阻塞(BRVO)危险因素的差异,为治疗和预防该病提供客观依据。方法:1) 所有研究对象选自2021年10月至2023年12月期间就诊于内蒙古自治区人民医院眼科的年龄 P > 0.05)。三组患者之间在Hcy、AT-III、胆固醇(CHOL)、叶酸、维生素B12、D-二聚体(D-D)、纤维蛋白降解产物(FDP)水平差异均有统计学意义(P P P < 0.001)。结论:1) Hcy水平升高和AT-III活性缺乏为中青年CRVO和BRVO的危险因素。2) 相对于BRVO患者,CRVO组中Hcy水平升高和AT-III缺乏的值更为显著。

影响II-III期结直肠癌患者生存的因素分析

分析影响II-III期结直肠癌患者生存的因素。方法 回顾性分析柳州市鹿寨县人民医院进行治疗的II-III期结直肠癌患者(110例),2017年5月至2021年8月为病例选取时间,并对所有纳入人员进行为期2年的随访,以分析影响其生存时间的因素。结果 性别、年龄、文化程度、烟酒史、家族史、肿瘤位置均P>0.05,非影响此类患者生存的高危指标;肿瘤分化程度、TNM分期、脉管侵犯、神经侵犯及手术方式均P<0.05,为影响此类患者生存的高危指标;COX逐步回归法结果显示,低肿瘤分化程度、TNM Ⅲ期、姑息性切除术、有脉管侵犯及神经侵犯均可作为影响II-III期结直肠癌患者生存的独立危险因素,P<0.05。结论 在II-III期结直肠癌患者中,低肿瘤分化程度、TNM Ⅲ期、姑息性切除术、有脉管侵犯及神经侵犯均可作为此疾病患者生存时间的影响因素,并可作为临床评估此疾病的相关指标。

经皮闭合复位四枚空心钉菱形内固定治疗PauwelsIII型股骨颈骨折

观察经皮闭合复位四枚空心钉菱形内固定治疗Pauwels III型股骨颈骨折的临床疗效。方法 将在我院(2022年1月至2023年12月)收治以Pauwels III型股骨颈骨折为诊断的患者总计60例,以随机数字表法分成两组各30例,其中以三枚空心钉行倒三角固定治疗患者30例为对照组,另30例经皮闭合复位四枚空心钉菱形内固定为研究组。比较两组手术前、后6个月髋关节Harris评分,手术指标,术后并发症。结果 术后6个月研究组Harris评分更高,研究组并发症率更低,差异有统计学意义(P<0.05)。结论 Pauwels III型股骨颈骨折以经皮闭合复位四枚空心钉菱形内固定,可改善髋关节功能,减少术后并发症,效果理想。

种植体支抗对快速扩弓后III类错颌患者上合骨A点的即刻变化影响研究

探讨种植体支抗对快速扩弓后III类错颌患者上合骨A点的即刻变化影响。方法 将受试者随机分为种植体支抗组和横腭杆组。对比分析不同组别治疗前后各项测量值的变化量以及所需治疗时间。结果 种植体支抗组的U1-Y, U6-Y, UL-EP, U1-SN各指标变化量与横腭杆组相比,差异具有统计学意义;反之,SNA, SNB, ANB各指标差异无统计学意义。在治疗时间上,种植体支抗组的结果与横腭杆组接近,差异不显著。结论 种植体支抗带来的即刻变化影响显著,有效改善了III类错颌患者的上合骨A点情况,而总体的治疗时间对比横腭杆组并无显著增加。这些建议种植体支抗应为快速扩弓后III类错颌患者的有效治疗手段。

改良前正中入路微型钢板内固定治疗II、III型尺骨冠状突骨折

对II、III型尺骨冠状突骨折通过改良前正中入路复位冠状突骨折块,微型钢板内固定的临床效果进行探讨。方法 分析我院骨伤科2018年8月至2020年8月的12例接受改良前正中入路,微型钢板内固定II、III型尺骨冠状突骨折患者的术中、术后等情况,并通过梅奥肘关节功能评分进行评定,以探讨该入路方式的临床效果。结果 手术后的随访时间平均超过6个月,达到平均8.2个月。所有患者的手术切口均成功愈合,骨折愈合时间在7-11周之间,平均为8.1周。肘部的活动度表现良好,平均屈曲活动度达到135.4°(110°~144°),伸直活动度平均为6°(-4°~13°),前臂的旋前和旋后活动度分别平均为76.2°(65°~86°)和80.3°(72°~84°)。根据梅奥肘关节功能评分,平均分为87.2分(74~100),其中有7例评为优,4例为良,1例为可,优良率达到91.7%。改良前正中入路治疗尺骨冠状突骨折表现出创伤小、暴露充分、安全性高、操作简单、关节功能恢复良好等优势,值得在临床中推广和应用。

Ni-CeO_(2)纳米复合材料的制备及其催化肼硼烷产氢的性能分析

开发高效的非贵金属催化剂用于肼硼烷(N2H4BH3,HB)制氢是实现其作为燃料电池氢源的关键。本文通过共沉淀法在活性中心Ni周围引入可以分散和稳定金属纳米粒子的CeO_(2),生成非贵金属催化剂Ni-CeO_(2),其通过调节金属和载体之间的相互作用,实现从肼硼烷中高效产氢。实验结果表明:共沉淀法制备得到的Ni-CeO_(2)相较于传统浸渍法制备得到的Ni/CeO_(2)-IMP有更优异的催化活性;Ni-CeO_(2)在323 K碱性条件下对肼硼烷产氢的TOF值为31.6 h^(-1),是Ni/CeO_(2)-IMP的3.2倍;Ni-CeO_(2)优异的催化活性归因于其更强的金属-载体相互作用(MSI),催化剂中存在更多的氧缺陷和碱性位点。通过本方法制备得到的Ni-MOx(M=Ce、La、Nd、Y、Mg、Zr和Al)可以很好地提升Ni对于肼硼烷产氢的活性以及氢气选择性,表明这种催化剂的合成策略具有普适性,有利于其在燃料电池中的实际应用。

大型三甲医院医疗信息化设计研究

为了提升大型三甲医院医疗服务效率与质量,优化患者就医体验,本文通过介绍大型三甲医院建设概况,深入剖析了医疗信息化技术架构的搭建原则与关键要素。进一步,文章详细设计了医疗信息化方案,包括医院信息集成平台的构建、信息化IT服务的全面覆盖,以及医院医疗信息化推进策略与逻辑衔接机制。作者提出,通过高度集成的信息系统与智能化的服务流程,能够有效促进医疗资源的合理配置与高效利用。研究结果表明,科学合理的医疗信息化设计对于推动大型三甲医院向智慧医疗转型、提升整体医疗服务水平具有显著作用。