Polysaccharide produced from medicinal endophytic fungus not only has applications in foods,but also exhibits multiple biological activities.In this work,an endophytic fungus Chaetomium globosum CGMCC 6882 could use c...Polysaccharide produced from medicinal endophytic fungus not only has applications in foods,but also exhibits multiple biological activities.In this work,an endophytic fungus Chaetomium globosum CGMCC 6882 could use corn stover to produce a polysaccharide(GCP-SC)by consolidated bioprocess and the titer of GCP-SC reached 3.2 g/L.The transcriptional levels of genes related to cellulose degradation(cbh,cdh,glu and egl)in C.globosum CGMCC 6882 were 4.38,3.85,3.13,and 2.17 folds compared to the control group when corn stover was used as the sole carbon source.Moreover,GCP-SC showed a time-and dose-dependent manner of inhibitory effect on A549 cells and the inhibitory rate reached 93.3%.This work provides meaningful data on agricultural residues utilization and facilitation of future relative resource conversion studies.展开更多
A new endophytic antagonistic fungus, Chaetomium spirale ND35 from Populus tomentosa, was reported. The bio-control trials of C. spirale ND35 against the Valsa Canker of apple were preliminarily investigated. The resu...A new endophytic antagonistic fungus, Chaetomium spirale ND35 from Populus tomentosa, was reported. The bio-control trials of C. spirale ND35 against the Valsa Canker of apple were preliminarily investigated. The results of dual culture on PDA plate showed that C. spirale ND35 was capable of strong antagonism against Valsa ceratosperma, and for inhibiting the mycelial growth of V. ceratosperma,.the crude extract of liquid culture of corn steep powder broth was more effective than that one of malt extract broth (MEB). The results of bio-control in greenhouse and field indicated that the disease incidence of apple tree treated with C. spirale ND35 was lower significantly than that treated by other methods. The re-isolation experiment suggested that C. spirale ND35 could colonize in stems and branches of apple trees successfully, and the ND35 colonization rate of the treatment with solid wheat bran culture was higher than that of corn steep powder broth, but the field experiment result the control effect of liquid culture of C. spirale ND35 was better than that of solid culture.展开更多
基金supported by the Natural Science Foundation of Youth Support Plan of Henan University of Technology (2017QNJH10)High Level Research Fund for Qualified People of Henan University of Technology (2017BS011)the Natural Science Foundation of Henan Province Education Department (19A180015)
文摘Polysaccharide produced from medicinal endophytic fungus not only has applications in foods,but also exhibits multiple biological activities.In this work,an endophytic fungus Chaetomium globosum CGMCC 6882 could use corn stover to produce a polysaccharide(GCP-SC)by consolidated bioprocess and the titer of GCP-SC reached 3.2 g/L.The transcriptional levels of genes related to cellulose degradation(cbh,cdh,glu and egl)in C.globosum CGMCC 6882 were 4.38,3.85,3.13,and 2.17 folds compared to the control group when corn stover was used as the sole carbon source.Moreover,GCP-SC showed a time-and dose-dependent manner of inhibitory effect on A549 cells and the inhibitory rate reached 93.3%.This work provides meaningful data on agricultural residues utilization and facilitation of future relative resource conversion studies.
基金This research was supported by National Natural Science Foundation of China (Grant No. 30100143)
文摘A new endophytic antagonistic fungus, Chaetomium spirale ND35 from Populus tomentosa, was reported. The bio-control trials of C. spirale ND35 against the Valsa Canker of apple were preliminarily investigated. The results of dual culture on PDA plate showed that C. spirale ND35 was capable of strong antagonism against Valsa ceratosperma, and for inhibiting the mycelial growth of V. ceratosperma,.the crude extract of liquid culture of corn steep powder broth was more effective than that one of malt extract broth (MEB). The results of bio-control in greenhouse and field indicated that the disease incidence of apple tree treated with C. spirale ND35 was lower significantly than that treated by other methods. The re-isolation experiment suggested that C. spirale ND35 could colonize in stems and branches of apple trees successfully, and the ND35 colonization rate of the treatment with solid wheat bran culture was higher than that of corn steep powder broth, but the field experiment result the control effect of liquid culture of C. spirale ND35 was better than that of solid culture.
