Dysregulation of mi R-124 has been reported to be involved in the pathophysiology of depression. Chaihu-Shugan-San, a traditional Chinese medicine, has antidepressive activity; however, the underlying mechanisms remai...Dysregulation of mi R-124 has been reported to be involved in the pathophysiology of depression. Chaihu-Shugan-San, a traditional Chinese medicine, has antidepressive activity; however, the underlying mechanisms remain unclear. In this study, to generate a rodent model of depression, rats were subjected to a combination of solitary confinement and chronic unpredictable mild stress for 28 days. Rats were intragastrically administered Chaihu-Shugan-San（2.835 m L/kg/d） for 4 weeks, once a day. Real-time reverse-transcription quantitative polymerase chain reaction, mi RNA microarray, western blot assay and transmission electron microscopy demonstrated that ChaihuShugan-San downregulated mi R-124 expression and upregulated the m RNA and protein levels of mitogen-activated protein kinase 14（MAPK14） and glutamate receptor subunit 3（Gria3）. Chaihu-Shugan-San also promoted synapse formation in the hippocampus. The open field test, sucrose consumption test and forced swimming test were used to assess depression-like behavior. After intragastric administration of Chaihu-Shugan-San, sucrose consumption increased, while the depressive behaviors were substantially reduced. Together, these findings suggest that Chaihu-Shugan-San exerts an antidepressant-like effect by downregulating mi R-124 expression and by releasing the inhibition of the MAPK14 and Gria3 signaling pathways.展开更多
OBJECTIVE:To investigate the mechanism underpinning the effect of Chaihu Shugan San(柴胡疏肝散,CHSGS)on major depressive disorder(MDD).METHODS:We searched the compound components of from seven herbal ingredients of CH...OBJECTIVE:To investigate the mechanism underpinning the effect of Chaihu Shugan San(柴胡疏肝散,CHSGS)on major depressive disorder(MDD).METHODS:We searched the compound components of from seven herbal ingredients of CHSGS from TCMSP,SymMap,ETCM,NPASS databases,and the chemical structure of the compound from PubChem,and collected the compound targets from TCMSP and TargetNet databases,and MDD-related targets from DiseaseGene Network.We established protein-protein interaction in the STRING database.Through gene mapping,topology analysis and enrichment analysis,the core targets and pathways of CHSGS for MDD,and the involved biological processes(BP),cell components(CC),and molecular functions(MF)were predicted.RESULTS:We collected a total of 1135 CHSGS compounds.After screening by ADME standards and the five rules of Ribinski,we obtained 99 different chemical components with different chemical structures,and related targets of 183 different CHSGS compounds.In the DiseaseGene Network,a total of 740 relevant targets for MDD were collected.Through gene mapping and topological analysis,62 related targets of CHSGS for MDD,24 targets with topological Chinese herbal medicine were obtained.Through enrichment analysis,10 relevant pathways and 3 core pathways were obtained with the involvement of 127 BP,27 CC,and 43 MF.CONCLUSION:There are multiple targets and signaling pathways are involved in the action of CHSGS in the treatment of MDD.展开更多
目的:探讨自我超越理论护理模式结合柴胡疏肝散加减在乳腺癌术后治疗中国的应用及对患者自护能力的影响。方法:选取乳腺癌术后患者72例,随机分为对照组和观察组,每组各36例。对照组采用乳腺癌术后常规护理,观察组在此基础上采用自我超...目的:探讨自我超越理论护理模式结合柴胡疏肝散加减在乳腺癌术后治疗中国的应用及对患者自护能力的影响。方法:选取乳腺癌术后患者72例,随机分为对照组和观察组,每组各36例。对照组采用乳腺癌术后常规护理,观察组在此基础上采用自我超越理论护理模式结合柴胡疏肝散加减治疗,术后共干预2周。对比干预前后两组Herth希望量表(herth hope index,HHI)、自我超越量表(Chinese version of the self-transcendence scale,CSTS)、汉密顿焦虑量表(Hamilton anxiety scale,HAMA)、汉密顿抑郁量表(Hamilton depression scale,HAMD)、自我护理能力量表评分及血清肿瘤标志物水平。结果:干预后,两组HHI、CSTS及自我护理能力评分均高于治疗前(P<0.05),HAMA、HAMD评分及血清肿瘤标志物水平均低于治疗前(P<0.05)。干预后观察组HHI、CSTS及自我护理能力评分均高于对照组(P<0.05),HAMA、HAMD评分及血清肿瘤标志物水平均低于对照组(P<0.05)。结论:自我超越理论护理模式结合柴胡疏肝散加减治疗,能有效提高乳腺癌患者术后的希望水平、自我超越水平及自我护理能力,缓解患者焦虑和抑郁情绪,降低血清肿瘤标志物水平,提高生活质量。展开更多
Objective:To analyze the effective components of Chinese medicine(CM)contained in Chaihu Shugan Powder(柴胡疏肝散,CSP)in the treatment of depressive disorders and to predict its anti-depressant mechanism by network ph...Objective:To analyze the effective components of Chinese medicine(CM)contained in Chaihu Shugan Powder(柴胡疏肝散,CSP)in the treatment of depressive disorders and to predict its anti-depressant mechanism by network pharmacology.Methods:Absorption,distribution,metabolism,excretion,and toxicity calculation method was used to screen the active components of CSP.Traditional Chinese Medicine System Pharmacological Database Analysis Platform and text mining tool(GoPuMed database)were used to predict and screen the active ingredients of CSP and anti-depressive targets.Through Genetic Association Database,Therapeutic Target Database,and PharmGkb database targets for depression were obtained.Cytoscape3.2.1 software was used to establish a network map of the active ingredients-targets of CSP,and to analyze gene function and metabolic pathways through Database for Annotation,Visualization and Integrated Discovery and the Omicshare database.Results:The 121 active ingredients and 15 depression-related targets which were screened from the database can exert antidepressant effects by improving the neural plasticity,growth,transfer condition and gene expression of neuronal cell,and the raise of the expression of gap junction protein.The 15 targets passed 14 metabolic pathways,mainly involved in the regulation of neurotransmitters(5-hydroxytryptamine,dopamine and epinephrine),inflammatory mediator regulation of TRP channels,calcium signaling pathway,cyclic adenosine monophosphate signaling pathway and neuroactive ligand-receptor interaction and other signal channels to exert anti-depressant effects.Conclusion:This article reveals the possible mechanism of CSP in the treatment of depression through network pharmacology research,and lays a foundation for further target studies.展开更多
目的:通过网络药理学方法探讨加味柴胡疏肝汤中君药和臣药配伍的抗癫痫作用机制,并通过分子对接技术预测药物有效成分与抗癫痫靶标的结合性。方法:从DisGeNET和GeneCards数据库中分别收集癫痫的靶标,取二者交集。在TCMSP数据库中检索加...目的:通过网络药理学方法探讨加味柴胡疏肝汤中君药和臣药配伍的抗癫痫作用机制,并通过分子对接技术预测药物有效成分与抗癫痫靶标的结合性。方法:从DisGeNET和GeneCards数据库中分别收集癫痫的靶标,取二者交集。在TCMSP数据库中检索加味柴胡疏肝汤中君药和臣药的有效成分及靶标。利用在线软件Venny进行映射得到加味柴胡疏肝汤君臣配伍抗癫痫作用的潜在靶标。通过STRING数据库构建基因功能关联网络。基于clusterProfiler包进行GO富集分析(gene ontology,GO)和KEGG富集分析(kyoto encyclopedia of genes and genomes,KEGG)。同时使用Cytoscape软件构建“成分-靶标-通路”关系网络。最后,通过Autodock Vina实现君臣配伍有效成分与癫痫靶标蛋白的分子对接。结果:加味柴胡疏肝汤君臣配伍的多种有效成分能够作用于癫痫的37个潜在靶标。这些潜在靶标主要影响了69个GO生物学功能。涉及了113条KEGG信号通路。此外,加味柴胡疏肝汤君臣配伍的有效成分均可与癫痫核心靶标自发地结合,且结合性良好。结论:加味柴胡疏肝汤君臣配伍的有效成分与癫痫相关的靶标具有良好的结合性。加味柴胡疏肝汤君臣配伍的有效成分可能是通过多靶标、多途径共同发挥抗癫痫作用的。展开更多
Objective： To investigate the protective effects of Chinese medicine formulation Chaihu Shugan San（柴胡疏肝散, CHSGS） on nonalcoholic fatty liver disease（NAFLD） in rats with insulin resistance（IR） and its molec...Objective： To investigate the protective effects of Chinese medicine formulation Chaihu Shugan San（柴胡疏肝散, CHSGS） on nonalcoholic fatty liver disease（NAFLD） in rats with insulin resistance（IR） and its molecular mechanisms. Methods： Male Sprague-Dawley rats were randomly divided into six groups： the control group, the model group, Dongbao Gantai group（东宝肝泰, DBGT, 0.09 g methionine/kg）, CHSGS high-dose group（CHSG-H, 12.6 g crude drug/kg）, CHSGS medium-dose group（CHSG-M, 6.3 g crude drug/kg）, and CHSGS low-dose group（CHSG-L, 3.15 g crude drug/kg）. After establishing the NAFLD rat model and treatment for 8 weeks, total cholesterol（TC）, triglyceride（TG）, high-density lipoprotein cholesterol（HDL-C）, free fatty acid（FFA）, fasting blood glucose（FBG）, fasting insulin（FINS） contents in blood serum, and TC, TG contents in the hepatic homogenate were measured by an automatic biochemical analyzer, and a homeostasis model assessment was applied to assess the status of IR, insulin sensitivity index（ISI）, and homeostasis model assessment for insulin secretion（HOMA-IS）. The expression levels of adiponectin and leptin mRNA in liver tissue were analyzed by reverse transcription polymerase chain reaction. Pathological changes of livers were observed by hematoxylineosin staining of paraffin section. Results： Compared with the model group, the serum levels of TC, TG, FFA, FBG, FINS, IRI, ISI, and the liver levels of TC and TG in CHSG-H, CHSG-M, CHSG-L groups showed significant declines（P〈0.01 or P〈0.05）; the serum levels of HDL-C, HOMA-IS were significantly increased（P〈0.01 or P〈0.05）; the expression of leptin mRNA was dramatically decreased and the expression of adiponectin mRNA was increased in the hepatic tissue（P〈0.01 or P〈0.05）. The fatty deposition of liver cells could also be alleviated. Conclusion： CHSGS could up-regulate the expression of adiponectin mRNA and down-regulate the expression of leptin mRNA on the liver, suggesting the CHSGS had positive therapeutic effect on NAFLD in rats with IR.展开更多
目的:运用网络药理学方法探讨柴胡疏肝散治疗乙型肝炎相关性肝癌的作用机制。方法:通过TCMSP平台筛选柴胡疏肝散活性成分及药物靶点,利用已知肝癌数据库(Onco DB.HCC数据库和Liverome数据库)及GEO数据库筛选出乙型肝炎相关性肝癌的疾病...目的:运用网络药理学方法探讨柴胡疏肝散治疗乙型肝炎相关性肝癌的作用机制。方法:通过TCMSP平台筛选柴胡疏肝散活性成分及药物靶点,利用已知肝癌数据库(Onco DB.HCC数据库和Liverome数据库)及GEO数据库筛选出乙型肝炎相关性肝癌的疾病靶点,用String构建蛋白质-蛋白质相互作用网络并筛选关键靶点,运用生物信息学方法对潜在靶点进行基因本体论(GO)分析和京都基因和基因组百科全书(KEGG)通路分析。利用GEPIA数据库对关键靶点进行生存预后分析。结果:获得柴胡疏肝散治疗乙型肝炎相关性肝癌的有效成分116个,潜在靶点17个,关键活性成分为槲皮素、山柰酚、β-谷甾醇、异鼠李素;关键靶点为雌激素受体1(Estrogen Receptor 1,ESR1)、细胞色素P4503A4酶(Cytochrome P450 Family 3 Subfamily A Member 4,CYP3A4)、醌氧化还原酶1[NAD(P)H Quinone Oxidoreductase 1,NQO1]。共涉及482个生物过程、5种细胞成分、57个分子功能,主要富集于12条信号通路。预测柴胡疏肝散主要通过干预类固醇激素的合成在乙型肝炎相关性肝癌的治疗中发挥重要的作用。结论:柴胡疏肝散通过多成分、多靶点、多途径治疗乙型肝炎相关性肝癌,且对抑郁也有干预作用,与中医疏肝解郁的治则相符。展开更多
基金supported by the National Natural Science Foundation of China,No.81503415,81574038,81603671the China Postdoctoral Science Foundation Grant,No.2016M600709+1 种基金a grant from the Science and Technology Planning Project of Guangdong Province of China,No.2014A020221062a grant from the Science and Technology Planning Project of Shenzhen City of China,No.JCYJ20150401170235349,JCYJ20160428105749954
文摘Dysregulation of mi R-124 has been reported to be involved in the pathophysiology of depression. Chaihu-Shugan-San, a traditional Chinese medicine, has antidepressive activity; however, the underlying mechanisms remain unclear. In this study, to generate a rodent model of depression, rats were subjected to a combination of solitary confinement and chronic unpredictable mild stress for 28 days. Rats were intragastrically administered Chaihu-Shugan-San（2.835 m L/kg/d） for 4 weeks, once a day. Real-time reverse-transcription quantitative polymerase chain reaction, mi RNA microarray, western blot assay and transmission electron microscopy demonstrated that ChaihuShugan-San downregulated mi R-124 expression and upregulated the m RNA and protein levels of mitogen-activated protein kinase 14（MAPK14） and glutamate receptor subunit 3（Gria3）. Chaihu-Shugan-San also promoted synapse formation in the hippocampus. The open field test, sucrose consumption test and forced swimming test were used to assess depression-like behavior. After intragastric administration of Chaihu-Shugan-San, sucrose consumption increased, while the depressive behaviors were substantially reduced. Together, these findings suggest that Chaihu-Shugan-San exerts an antidepressant-like effect by downregulating mi R-124 expression and by releasing the inhibition of the MAPK14 and Gria3 signaling pathways.
基金Supported by the General Program of National Natural Science Foundation of China(No.81774122)Young Teachers Project of Beijing University of Chinese Medicine(No.2017-JYB-JS-002),College Level Project of Beijing University of Chinese Medicine(No.2019-JYB-XS-002)+1 种基金Projects of Sichuan Administration of Traditional Chinese Medicine(No.2020JC0035)Xinglin Scholar Project of Chengdu University of Traditional Chinese Medicine(No.BSH2020016)。
文摘OBJECTIVE:To investigate the mechanism underpinning the effect of Chaihu Shugan San(柴胡疏肝散,CHSGS)on major depressive disorder(MDD).METHODS:We searched the compound components of from seven herbal ingredients of CHSGS from TCMSP,SymMap,ETCM,NPASS databases,and the chemical structure of the compound from PubChem,and collected the compound targets from TCMSP and TargetNet databases,and MDD-related targets from DiseaseGene Network.We established protein-protein interaction in the STRING database.Through gene mapping,topology analysis and enrichment analysis,the core targets and pathways of CHSGS for MDD,and the involved biological processes(BP),cell components(CC),and molecular functions(MF)were predicted.RESULTS:We collected a total of 1135 CHSGS compounds.After screening by ADME standards and the five rules of Ribinski,we obtained 99 different chemical components with different chemical structures,and related targets of 183 different CHSGS compounds.In the DiseaseGene Network,a total of 740 relevant targets for MDD were collected.Through gene mapping and topological analysis,62 related targets of CHSGS for MDD,24 targets with topological Chinese herbal medicine were obtained.Through enrichment analysis,10 relevant pathways and 3 core pathways were obtained with the involvement of 127 BP,27 CC,and 43 MF.CONCLUSION:There are multiple targets and signaling pathways are involved in the action of CHSGS in the treatment of MDD.
文摘目的:探讨自我超越理论护理模式结合柴胡疏肝散加减在乳腺癌术后治疗中国的应用及对患者自护能力的影响。方法:选取乳腺癌术后患者72例,随机分为对照组和观察组,每组各36例。对照组采用乳腺癌术后常规护理,观察组在此基础上采用自我超越理论护理模式结合柴胡疏肝散加减治疗,术后共干预2周。对比干预前后两组Herth希望量表(herth hope index,HHI)、自我超越量表(Chinese version of the self-transcendence scale,CSTS)、汉密顿焦虑量表(Hamilton anxiety scale,HAMA)、汉密顿抑郁量表(Hamilton depression scale,HAMD)、自我护理能力量表评分及血清肿瘤标志物水平。结果:干预后,两组HHI、CSTS及自我护理能力评分均高于治疗前(P<0.05),HAMA、HAMD评分及血清肿瘤标志物水平均低于治疗前(P<0.05)。干预后观察组HHI、CSTS及自我护理能力评分均高于对照组(P<0.05),HAMA、HAMD评分及血清肿瘤标志物水平均低于对照组(P<0.05)。结论:自我超越理论护理模式结合柴胡疏肝散加减治疗,能有效提高乳腺癌患者术后的希望水平、自我超越水平及自我护理能力,缓解患者焦虑和抑郁情绪,降低血清肿瘤标志物水平,提高生活质量。
基金Supported by Jiangsu Administration of Traditional Chinese Medicine(No.YB2015052)Scientific Research Project of Jiangsu Health Commission(No.Z2018005)Jiangsu Provincial Bureau of Traditional Chinese Medicine"Chinese Medicine Encephalopathy"Key Discipline Open Project。
文摘Objective:To analyze the effective components of Chinese medicine(CM)contained in Chaihu Shugan Powder(柴胡疏肝散,CSP)in the treatment of depressive disorders and to predict its anti-depressant mechanism by network pharmacology.Methods:Absorption,distribution,metabolism,excretion,and toxicity calculation method was used to screen the active components of CSP.Traditional Chinese Medicine System Pharmacological Database Analysis Platform and text mining tool(GoPuMed database)were used to predict and screen the active ingredients of CSP and anti-depressive targets.Through Genetic Association Database,Therapeutic Target Database,and PharmGkb database targets for depression were obtained.Cytoscape3.2.1 software was used to establish a network map of the active ingredients-targets of CSP,and to analyze gene function and metabolic pathways through Database for Annotation,Visualization and Integrated Discovery and the Omicshare database.Results:The 121 active ingredients and 15 depression-related targets which were screened from the database can exert antidepressant effects by improving the neural plasticity,growth,transfer condition and gene expression of neuronal cell,and the raise of the expression of gap junction protein.The 15 targets passed 14 metabolic pathways,mainly involved in the regulation of neurotransmitters(5-hydroxytryptamine,dopamine and epinephrine),inflammatory mediator regulation of TRP channels,calcium signaling pathway,cyclic adenosine monophosphate signaling pathway and neuroactive ligand-receptor interaction and other signal channels to exert anti-depressant effects.Conclusion:This article reveals the possible mechanism of CSP in the treatment of depression through network pharmacology research,and lays a foundation for further target studies.
文摘目的:通过网络药理学方法探讨加味柴胡疏肝汤中君药和臣药配伍的抗癫痫作用机制,并通过分子对接技术预测药物有效成分与抗癫痫靶标的结合性。方法:从DisGeNET和GeneCards数据库中分别收集癫痫的靶标,取二者交集。在TCMSP数据库中检索加味柴胡疏肝汤中君药和臣药的有效成分及靶标。利用在线软件Venny进行映射得到加味柴胡疏肝汤君臣配伍抗癫痫作用的潜在靶标。通过STRING数据库构建基因功能关联网络。基于clusterProfiler包进行GO富集分析(gene ontology,GO)和KEGG富集分析(kyoto encyclopedia of genes and genomes,KEGG)。同时使用Cytoscape软件构建“成分-靶标-通路”关系网络。最后,通过Autodock Vina实现君臣配伍有效成分与癫痫靶标蛋白的分子对接。结果:加味柴胡疏肝汤君臣配伍的多种有效成分能够作用于癫痫的37个潜在靶标。这些潜在靶标主要影响了69个GO生物学功能。涉及了113条KEGG信号通路。此外,加味柴胡疏肝汤君臣配伍的有效成分均可与癫痫核心靶标自发地结合,且结合性良好。结论:加味柴胡疏肝汤君臣配伍的有效成分与癫痫相关的靶标具有良好的结合性。加味柴胡疏肝汤君臣配伍的有效成分可能是通过多靶标、多途径共同发挥抗癫痫作用的。
基金Suppport by the National Natural Science Foundation of China(No.30973913)the Science Foundation of Science and Technology Bureau of Guangdong(No.2008A030101005)
文摘Objective： To investigate the protective effects of Chinese medicine formulation Chaihu Shugan San（柴胡疏肝散, CHSGS） on nonalcoholic fatty liver disease（NAFLD） in rats with insulin resistance（IR） and its molecular mechanisms. Methods： Male Sprague-Dawley rats were randomly divided into six groups： the control group, the model group, Dongbao Gantai group（东宝肝泰, DBGT, 0.09 g methionine/kg）, CHSGS high-dose group（CHSG-H, 12.6 g crude drug/kg）, CHSGS medium-dose group（CHSG-M, 6.3 g crude drug/kg）, and CHSGS low-dose group（CHSG-L, 3.15 g crude drug/kg）. After establishing the NAFLD rat model and treatment for 8 weeks, total cholesterol（TC）, triglyceride（TG）, high-density lipoprotein cholesterol（HDL-C）, free fatty acid（FFA）, fasting blood glucose（FBG）, fasting insulin（FINS） contents in blood serum, and TC, TG contents in the hepatic homogenate were measured by an automatic biochemical analyzer, and a homeostasis model assessment was applied to assess the status of IR, insulin sensitivity index（ISI）, and homeostasis model assessment for insulin secretion（HOMA-IS）. The expression levels of adiponectin and leptin mRNA in liver tissue were analyzed by reverse transcription polymerase chain reaction. Pathological changes of livers were observed by hematoxylineosin staining of paraffin section. Results： Compared with the model group, the serum levels of TC, TG, FFA, FBG, FINS, IRI, ISI, and the liver levels of TC and TG in CHSG-H, CHSG-M, CHSG-L groups showed significant declines（P〈0.01 or P〈0.05）; the serum levels of HDL-C, HOMA-IS were significantly increased（P〈0.01 or P〈0.05）; the expression of leptin mRNA was dramatically decreased and the expression of adiponectin mRNA was increased in the hepatic tissue（P〈0.01 or P〈0.05）. The fatty deposition of liver cells could also be alleviated. Conclusion： CHSGS could up-regulate the expression of adiponectin mRNA and down-regulate the expression of leptin mRNA on the liver, suggesting the CHSGS had positive therapeutic effect on NAFLD in rats with IR.
文摘目的:运用网络药理学方法探讨柴胡疏肝散治疗乙型肝炎相关性肝癌的作用机制。方法:通过TCMSP平台筛选柴胡疏肝散活性成分及药物靶点,利用已知肝癌数据库(Onco DB.HCC数据库和Liverome数据库)及GEO数据库筛选出乙型肝炎相关性肝癌的疾病靶点,用String构建蛋白质-蛋白质相互作用网络并筛选关键靶点,运用生物信息学方法对潜在靶点进行基因本体论(GO)分析和京都基因和基因组百科全书(KEGG)通路分析。利用GEPIA数据库对关键靶点进行生存预后分析。结果:获得柴胡疏肝散治疗乙型肝炎相关性肝癌的有效成分116个,潜在靶点17个,关键活性成分为槲皮素、山柰酚、β-谷甾醇、异鼠李素;关键靶点为雌激素受体1(Estrogen Receptor 1,ESR1)、细胞色素P4503A4酶(Cytochrome P450 Family 3 Subfamily A Member 4,CYP3A4)、醌氧化还原酶1[NAD(P)H Quinone Oxidoreductase 1,NQO1]。共涉及482个生物过程、5种细胞成分、57个分子功能,主要富集于12条信号通路。预测柴胡疏肝散主要通过干预类固醇激素的合成在乙型肝炎相关性肝癌的治疗中发挥重要的作用。结论:柴胡疏肝散通过多成分、多靶点、多途径治疗乙型肝炎相关性肝癌,且对抑郁也有干预作用,与中医疏肝解郁的治则相符。
