With the increase in antimicrobial resistance,it has become necessary to explore alternative approaches for combating and preventing diseases.DB-cinnamaldehyde(CNM)and Benzyl4-amino(B4AM)are bioactive compounds derive...With the increase in antimicrobial resistance,it has become necessary to explore alternative approaches for combating and preventing diseases.DB-cinnamaldehyde(CNM)and Benzyl4-amino(B4AM)are bioactive compounds derived from chalcones but with restricted solubility in aqueous media.Nanoemulsions can enhance the solubility of compounds and can be a promising alternative in the development of novel antimicrobials,with reduced side effects and prolonged release.The objective of this study was to evaluate the stability of oil-in-water nanoemulsions loaded with two distinct types of chalcones at two different dosages,to propose a stable formulation with antimicrobial properties.Results showed that nanoemulsions presented high encapsulation efficiency,low polydispersity index(PDI)and particle size below 200 nm,indicating that emulsification was a suitable method for nanoemulsion preparation.Nanoemulsions with higher dosages exhibited significant antimicrobial effects when compared to free chalcones and positive controls.Notably,B4AM nanoemulsions at higher dosages showed expressive activity against Salmonella minnesota,with a 420%greater inhibitory response compared to the free form and showing equivalence to the positive control.CNM nanoemulsions showed excellent inhibitory activity at the highest dosage,equivalent to the positive control against S.minnesota and Staphylococcus aureus.The greater number of conjugated bonds in CNM increased the antimicrobial activity in comparison with B4AM,and the formation of nanometric domains enhanced the bioavailability,being a promising alternative for antimicrobial applications.展开更多
UV-VIS spectroscopic investigations of interaction of bovine and human serum albumin with selected chalcones (1) and their cyclic chalcone analogues: (E)-2-(4’-X-benzylidene-1-tetralones (3), benzosuberones (4) with ...UV-VIS spectroscopic investigations of interaction of bovine and human serum albumin with selected chalcones (1) and their cyclic chalcone analogues: (E)-2-(4’-X-benzylidene-1-tetralones (3), benzosuberones (4) with dimethylamino and methoxy substituents and (E)-2-(2’,4’-dimethox- ybenzylidene)-1-indanone (2) were performed in polar respiration medium. Absorption maxima of the tested compounds were investigated in the presence of bovine and human serum albumin at the 0, 10, 30 and 60 minute timepoints of the interaction. The absorbance of all studied compounds in the presence of proteins decreased after one hour of the reaction. Molecule 4a showed the strongest and fastest kinet initial interaction with both albumins.展开更多
Objective: To investigate in vitro antimalarial activity of chalcone derivative compounds against Plasmodum falciparum 3D7(Pf3D7) strain and in silico antimalarial activity.Methods: Synthesis of the chalcone derivativ...Objective: To investigate in vitro antimalarial activity of chalcone derivative compounds against Plasmodum falciparum 3D7(Pf3D7) strain and in silico antimalarial activity.Methods: Synthesis of the chalcone derivatives was conducted via Claisen-Schmidt method using NaOH 60% base as catalyst. An in vitro antimalarial activity assay was carried out according to the Rieckmann method against the chloroquine-sensitive Pf3D7 strain. Molecular docking studies of the prepared compounds were performed using Discovery Studio 3.1(Accelrys, Inc., San Diego, USA) software to dihydrofolate reductases-thymidylate synthase(PfDHFR-TS) protein with Protein Data Bank ID of 1J3I.pdb(sensitive-protein) and ID: 4DP3.pdb(resistance-protein).Results: This work has successfully synthesized seven chalcone derivatives with a great antimalarial activity. It has been revealed that allyloxy, hydroxy and alkoxy functional groups could increase the antimalarial activity of the chalcone derivatives. The best antimalarial activity of the prepared compounds was possessed by 3b with an IC_(50) value of 0.59 μM and categorized as an excellent antiplasmodial. Molecular docking studies of 3b showed binding interaction with the amino acid residues such as Ala16. Ile 164. Phe58.Tyr170 of the 1J3I.pdb protein and also Ala16, Phe58, Ile 112, Met55 of the 4DP3.pdb protein.Conclusions: An in vitro antimalarial assay of the prepared chalcone derivative(3a-g)showed an excellent and good antiplasmodial activity against the chloroquine-sensitive Pf3D7 strain. In silico antimalarial studies revealed that 3a-g made binding interaction with both sensitive-protein(IJ3I.pdb) and resistance-protein(4DP3.pdb), which means that they were both active against chloroquine-sensitive and resistant plasmodium strain.展开更多
In the flavonoid biosynthesis pathway, Chalcone synthase (CHS) is involved in the formation of the pigment and has been shown to be a rate-limiting enzyme for the synthesis of flavonoids. In this study, a PCR approach...In the flavonoid biosynthesis pathway, Chalcone synthase (CHS) is involved in the formation of the pigment and has been shown to be a rate-limiting enzyme for the synthesis of flavonoids. In this study, a PCR approach was used to clone a Chalcone synthases cDNA from flower of sweet osmanthus “Chenghong Dangui” and it was designated as OfCHS (O. fragrans, CHS). The cDNA was 1383 bp long and a coding sequence (CDS) of 1173 bp encoding a polypeptide of 391 amino acids with an estimated molecular mass of 39.9 kDa. The theoretical isoelectric point was 6.23. Phylogenetic analysis demonstrated that OfCHS clustered with Olea europaea, Solenostemon scutellarioides, Perilla frutescens, Antirrhinum majus and Digitalis lanata. We also detected the expression of OfCHS in different tissues in “Dangui” and in two cultivars with varied coloration, “Zi Yingui” and “Chenghong Dangui” at different floral stages using quantitative real-time PCR. We observed that OfCHS transcript was higher in leaves than in petals in “Dangui”. The transcripts of OfCHS in “Zi Yingui” petals were higher than those in “Dangui” at three stages especially at xianyan stage and there was no significant difference between the two cultivars in the full flowering stage. “Chenghong Dangui” has a relatively high anthocyanin content compared to “Zi Yingui”. The relative amount of anthocyanin of “Chenghong Dangui” initially increases, and then decreases during the bloom period. However, the expression of CHS is the highest at the initial flowering stage. These data suggest that the OfCHS does not play a key role in the accumulation of total flavonoid in this cultivar. These data could contribute to explain the different accumulation of flavonoids in petals of the two cultivars.展开更多
An ionic liquid(IL)catalyzed solvent-free process was developed for the direct synthesis of chalcone and its derivatives by using substituted acetophenones and benzaldehydes via aldol reaction under mild conditions.A ...An ionic liquid(IL)catalyzed solvent-free process was developed for the direct synthesis of chalcone and its derivatives by using substituted acetophenones and benzaldehydes via aldol reaction under mild conditions.A series of acidic and basic ILs were selected and screened.The influences of cations and reaction conditions on product yield and selectivity were systematically investigated.The[Bmim]OH was identified as the optimal IL,with the highest yield and selectivity reaching up to 96.7% and 100%,respectively.A reaction mechanism-based kinetic model was established and regressed with experimental data,revealing the β-Hydroxylketone dehydrolysis with activation barrier of 37.8 kJ·mol^(-1) was observed as the ratecontrolling step.展开更多
Chalcone synthases (CHS, EC 2.3.1.74) are key enzymes that catalyze the first committed step in flavonoid biosynthesis. In this study, we isolated a chalcone synthase, named NtCHS6, from Nicotiana tabacum. This synt...Chalcone synthases (CHS, EC 2.3.1.74) are key enzymes that catalyze the first committed step in flavonoid biosynthesis. In this study, we isolated a chalcone synthase, named NtCHS6, from Nicotiana tabacum. This synthase shared high homology with the NSCHSL (Y14507) gene and contained most of the conserved active sites that are in CHS proteins. The phylogenetic analysis suggested that NtCHS6 protein shared a large genetic distance with other Solanaceae CHS proteins and was the most closely-related to an uncharacterized CHS from Solanum lycopersicum. The expression analysis indicated that NtCHS6 was abundantly expressed in leaves, especially in mature leaves. By scrutinizing its upstream promoter sequences, multiple cis-regulatory elements involved in light and drought responsive were detected. Furthermore, NtCHS6 expression decreased significantly under dark treatment and increased significantly under drought stress suggested that NtCHS6 expression exhibited both light responsiveness and drought responsiveness, and important roles in ultraviolet protection and drought tolerance. Our results might play展开更多
Chalcone is a plant metabolite widely found in fruits,vegetables,spices and tea,and has anti-tumor,anti-inflammation,immunomodulation,antibacterial and anti-oxidation activities,as well as many other pharmacological a...Chalcone is a plant metabolite widely found in fruits,vegetables,spices and tea,and has anti-tumor,anti-inflammation,immunomodulation,antibacterial and anti-oxidation activities,as well as many other pharmacological and biological effects.Our team has shown that its analogs have antioxidant activity,and oxidative stress is a pathological hallmark of retinal ischemia/reperfusion injury that can lead to retinal damage and visual loss.This investigation aims to identify a chalcone that protects retinal ganglion cells in vitro from the effects of oxidative stress and examine its mechanism.Rat retinal ganglion cell-5 cells were pretreated with chalcones and then exposed to tert-butyl hydroperoxide that causes oxidative damage.Controls received dimethyl sulfoxide only or tert-butyl hydroperoxide in dimethyl sulfoxide.Only(E)-3,4-dihydroxy-2′-methylether ketone(L2 H17),of the five chalcone analogs,markedly increased the survival rate of oxidatively injured RGC-5 cells.Thus,subsequent experiments only analyzed the results of the L2 H17 intervention.Cell viability and apoptosis were measured.Intracellular superoxide dismutase and reactive oxygen species levels were used to assess induced oxidative stress.The mechanism of action by L2 H17 was explored by measuring the ER stress/UPR pathway and the expression and localization of Nrf2.All results demonstrated that L2 H17 could reduce the apoptosis of oxidatively injured cells,inhibit caspase-3 activity,increase Bcl-2 expression,decrease Bad expression,increase the activity of superoxide dismutase,inhibit the production of reactive oxygen species,increase Nrf2 immunoreactivity,and reduce the activating transcription factor 4,phospho-eukaryotic initiation factor 2 and CHOP expression.L2 H17 protects retinal ganglion cells induced by oxidative stress by regulating Nrf2,which indicates that it has the potential to become a drug for retinal ischemia/reperfusion.展开更多
In order to accelerate the development of relatively inexpensive antimalarials that are effective against chloroquine-resistant strains of Plasmodium falciparum, a methodology for the solid phase synthesis of chalcone...In order to accelerate the development of relatively inexpensive antimalarials that are effective against chloroquine-resistant strains of Plasmodium falciparum, a methodology for the solid phase synthesis of chalcone (1, 3-diphenyl-2-propen-1-one) analogues in reasonably high yields has been developed.展开更多
Corrosion inhibition of Al and Al-3.5Mg alloy by organic compounds, namely chalcones in hydrochloric acid solutions has been investigated by rapid polarization technique and weight loss method. Polarization measuremen...Corrosion inhibition of Al and Al-3.5Mg alloy by organic compounds, namely chalcones in hydrochloric acid solutions has been investigated by rapid polarization technique and weight loss method. Polarization measurements show that, the inhibitors act cathodically both in case of Al and Al-3.5Mg alloy. It was found from the weight loss measurements that, the inhibition efficiency depends on the substituent in the chalcone compound. The relative inhibitive efficiency of these compounds has been explained on the basis of structure dependent electron donor properties of the inhibitors and the metal inhibitor interaction on the surface. The inhibition efficiency ranges from 16 to 64% for Al and from 30% to 91% for Al-3.5Mg alloy展开更多
Several dimethylamino-substituted chalcone (i .e. dimethylaminobenzal acetophenone) (DBA) derivatives with intramolecular charge transfer transition character were used as visible light sensitizers for radical photopo...Several dimethylamino-substituted chalcone (i .e. dimethylaminobenzal acetophenone) (DBA) derivatives with intramolecular charge transfer transition character were used as visible light sensitizers for radical photopolymerization initiated by iodonium salt (DPIO). Initiating radical species is produced from DBA sensitized photolysis of DPIO through the single electron transfer, accompanying the bleaching of DBA, The activity of DBA decreases as a function of substituent attached to phenyl ring in the order: DBA-2 (OCH_3) >DBA-1(H)> DBA-3 (Cl). The kinetic study on photopolymerization of MMA was carried out in CH_3CN solution at 30 ℃ by dilatometry. The polymerization rate was determined to be proportional to the concentration with exponents of 0.42, 0.25 and 0.86 for DPIO, DBA-1 and MMA, respectively.展开更多
A novel chalcone derivative was isolated from Onychium japonicum and its structure was elucidated by spectroscopic methods.The isolated compound showed cytotoxic activity against the human carcinoma cell lines Hela an...A novel chalcone derivative was isolated from Onychium japonicum and its structure was elucidated by spectroscopic methods.The isolated compound showed cytotoxic activity against the human carcinoma cell lines Hela and BEL-7402 in vitro.展开更多
Reaction of heterocycle substituted telluronium salts with aroma- tic aldehydes under mild conditions gave corresponding chalcones.In the presence of dibutyl telluride,2-bromomethyl-5-nitrofuran condensed easily with ...Reaction of heterocycle substituted telluronium salts with aroma- tic aldehydes under mild conditions gave corresponding chalcones.In the presence of dibutyl telluride,2-bromomethyl-5-nitrofuran condensed easily with aromatic aldehydes in THF to afford corresponding olefin derivatives as one-pot reaction.展开更多
The SHG effects of substituted 3-thienyl chalcone compounds weremeasured. The relationship between structure and effect of substituted 3-thienyl chal-cone derivatives and substituted 2-thienyl chalcone derivatives wer...The SHG effects of substituted 3-thienyl chalcone compounds weremeasured. The relationship between structure and effect of substituted 3-thienyl chal-cone derivatives and substituted 2-thienyl chalcone derivatives were discussed. It isshown that their properties of high SHG efficient are similar to each other and the sub-stituted 3-thienyl chalcone molecules are easier to form non-centrosymmetric crystalstructure than substituted 2-thienyl chalcone molecules.展开更多
A simple and rapid method was developed using cyclic, differential pulse and square wave voltammetric techniques for the determination of trace-level chalcone at a glassy carbon electrode. Chalcone could produce two a...A simple and rapid method was developed using cyclic, differential pulse and square wave voltammetric techniques for the determination of trace-level chalcone at a glassy carbon electrode. Chalcone could produce two anodic peaks at about 0.514 V and 1.478 V and a cathodic peak at about -0.689 V. The differential pulse voltammerty presents a good linear response as compared to square wave voltammetry in the range of 0.2 - 10 μM with a detection limit of 0.18 μM. The proposed method was used successfully for its quantitative determination in spiked human plasma and urine as real samples.展开更多
Based on the antitumor activity of chalcone derivatives and their structure, the structure-activity relationship of chalcone derivatives was analyzed by CoMFA (comparative molecular field analysis) method and then the...Based on the antitumor activity of chalcone derivatives and their structure, the structure-activity relationship of chalcone derivatives was analyzed by CoMFA (comparative molecular field analysis) method and then the 3D-QSAR (the three dimensional quantitative structures activity relationship) model was established. The analytical results showed that the model had good stability and prediction ability with cross-validated Q2 and non-cross-validated R2 values of 0.527 and 0.995, respectively. The contour map of the model explained the relationship between the structure of chalcone derivatives and antitumor activity, and could be analyzed to design antitumor chalcone derivatives. We designed some structure of chalcone derivatives and calculated their antitumor activity. In this paper, 24 chalcone derivatives were studied by CoMFA, 3D-QSAR, molecular design, which provided theoretical for designing good activities chalcone derivatives.展开更多
A systematic conceptual density functional theory (DFT) analysis was performed on a series of chlorinated chalcones to study the effect of electron distribution on antimicrobial activity. In our previous work, a serie...A systematic conceptual density functional theory (DFT) analysis was performed on a series of chlorinated chalcones to study the effect of electron distribution on antimicrobial activity. In our previous work, a series of 16 chlorinated chalcones were synthesized to determine the antimicrobial effects of varying the location of the halogen substituent on each aromatic ring of the chalcone. Herein is reported a DFT investigation of those 16 chalcones and a comparison of quantum chemical properties to their antimicrobial activity. DFT global chemical reactivity descriptors (chemical hardness/softness, chemical potential/electronegativity, and electrophilicity) and local reactivity descriptors (Fukui functions and dual descriptor) were calculated for all compounds using Spartan’18 software. All calculations were carried out at the B3LYP/6-31G* level of theory. Reactivity analysis of the Fukui dual descriptor calculations reveals sites of nucleophilic and electrophilic attack. These in-silico results provide a foundation for further synthetic optimization of the chalcone skeleton to serve as novel antimicrobial agents.展开更多
This paper summarizes the synthesis, growth and the effect of the position of the substituent in the thienyl ring and also the properties of the grown chalcone crystals, 2-CTP and 3-CTP. The formation of compound is c...This paper summarizes the synthesis, growth and the effect of the position of the substituent in the thienyl ring and also the properties of the grown chalcone crystals, 2-CTP and 3-CTP. The formation of compound is confirmed by the re- corded H1NMR spectra. A FT-IR spectrum confirms the presence of all functional groups in both of the crystals. Single crystal XRD reports that even though these two compounds crystallize in monoclinic crystal system, 2-CTP has centro- symmetric P21/c space group and 3-CTP has non-centrosymmetric space group P21. Thermal properties of grown crys- tals analyzed by TG/DTA study explain that the 3-CTP compound is slightly more stable than 2-CTP. The transparency of these isomers in the vis-IR region has been studied. Second and third order nonlinear optical properties of 3-CTP and 2-CTP crystals have been investigated by powder SHG and Z-scan technique respectively.展开更多
Background: Chalcones are open-chain flavonoids which display a large number of pharmacological activities such as cytotoxic, anti-inflammatory including antioxidant. The objective of this study was to assess antioxid...Background: Chalcones are open-chain flavonoids which display a large number of pharmacological activities such as cytotoxic, anti-inflammatory including antioxidant. The objective of this study was to assess antioxidant and cytotoxic activity of six synthesized chalcones. Methodology: For the current experiments, 1,3-diphenylpropenone (compound R) was used as molecular model to synthetize six compounds, namely three benzyl-benzimidazolyl-chalcones (U1, U2, WAC1) and three imidazopyridinyl-chalcones (V1, V2, V3). All the compounds were evaluated for their ability to scavenge the stable free ABTS.+ radical cation, according to the method develop by Choong et al. In addition, the cytotoxicity test described by Price et al., was performed using healthy human cell line, then in human malignant cell lines (HEP-2, A549). Results: All synthesized chalcones reduced the ABTS.+ radical cation. Indeed, benzyl benzimidazolyl compounds WAC1, U1, U2, by developing respectively 39.61%, 66.09%, and 84.20% percentages of reduction, showed an antioxidant effect 6, 11 and 14 times greater than the compound R (6.14%). As a result, imidazopyridinyl-chalcones compounds, namely V1, V2 and V3 reduced the ABTS.+ radical cation at 91.62%, 99.84% and 97.45% respectively, being 15 and 16 times more active than the compound R. About cytotoxicity, V2 inhibited not significantly HEP-2 malignant cells growth at 48.64%, compared to the standard product, i.e. doxorubicin that inhibited the growth of the same cells at 42.37%. WAC1 inhibited significantly the growth of A549 malignant cells at 89.53%, more than doxorubicin which percentage of growth inhibition was 71.58%. Conclusion: The presence of the α, β-unsaturated carbonyl system (or 1,3-diphenylpropenone) along with a benzimidazole or imidazopyridine heterocyclic ring is likely to contribute to both cytotoxic and antioxidant activities of these compounds.展开更多
基金supported by the National Council for Scientific Development-CNPQ/MCTI/FNDCT N18/2021,by the Concession of Research Funding Grant Number 406522/2021-9-1 to F.O.M.S.Abreu.Helcio Silva dos Santos acknowledges financial support from CNPq-PQ (Grant Number 306008/2022-0).
文摘With the increase in antimicrobial resistance,it has become necessary to explore alternative approaches for combating and preventing diseases.DB-cinnamaldehyde(CNM)and Benzyl4-amino(B4AM)are bioactive compounds derived from chalcones but with restricted solubility in aqueous media.Nanoemulsions can enhance the solubility of compounds and can be a promising alternative in the development of novel antimicrobials,with reduced side effects and prolonged release.The objective of this study was to evaluate the stability of oil-in-water nanoemulsions loaded with two distinct types of chalcones at two different dosages,to propose a stable formulation with antimicrobial properties.Results showed that nanoemulsions presented high encapsulation efficiency,low polydispersity index(PDI)and particle size below 200 nm,indicating that emulsification was a suitable method for nanoemulsion preparation.Nanoemulsions with higher dosages exhibited significant antimicrobial effects when compared to free chalcones and positive controls.Notably,B4AM nanoemulsions at higher dosages showed expressive activity against Salmonella minnesota,with a 420%greater inhibitory response compared to the free form and showing equivalence to the positive control.CNM nanoemulsions showed excellent inhibitory activity at the highest dosage,equivalent to the positive control against S.minnesota and Staphylococcus aureus.The greater number of conjugated bonds in CNM increased the antimicrobial activity in comparison with B4AM,and the formation of nanometric domains enhanced the bioavailability,being a promising alternative for antimicrobial applications.
基金thanks to the Austrian grant(ASO)SK-06/07-14/2007the Faculty of Mediccine Research Fund(University of Pécs)AOK-KA-213/20.
文摘UV-VIS spectroscopic investigations of interaction of bovine and human serum albumin with selected chalcones (1) and their cyclic chalcone analogues: (E)-2-(4’-X-benzylidene-1-tetralones (3), benzosuberones (4) with dimethylamino and methoxy substituents and (E)-2-(2’,4’-dimethox- ybenzylidene)-1-indanone (2) were performed in polar respiration medium. Absorption maxima of the tested compounds were investigated in the presence of bovine and human serum albumin at the 0, 10, 30 and 60 minute timepoints of the interaction. The absorbance of all studied compounds in the presence of proteins decreased after one hour of the reaction. Molecule 4a showed the strongest and fastest kinet initial interaction with both albumins.
基金Ministry of Research,Technology,and Higher Education-Indonesia for the financial support of this work through Hibah Penelitian Disertasi Doktor (PDD)T.A 2017
文摘Objective: To investigate in vitro antimalarial activity of chalcone derivative compounds against Plasmodum falciparum 3D7(Pf3D7) strain and in silico antimalarial activity.Methods: Synthesis of the chalcone derivatives was conducted via Claisen-Schmidt method using NaOH 60% base as catalyst. An in vitro antimalarial activity assay was carried out according to the Rieckmann method against the chloroquine-sensitive Pf3D7 strain. Molecular docking studies of the prepared compounds were performed using Discovery Studio 3.1(Accelrys, Inc., San Diego, USA) software to dihydrofolate reductases-thymidylate synthase(PfDHFR-TS) protein with Protein Data Bank ID of 1J3I.pdb(sensitive-protein) and ID: 4DP3.pdb(resistance-protein).Results: This work has successfully synthesized seven chalcone derivatives with a great antimalarial activity. It has been revealed that allyloxy, hydroxy and alkoxy functional groups could increase the antimalarial activity of the chalcone derivatives. The best antimalarial activity of the prepared compounds was possessed by 3b with an IC_(50) value of 0.59 μM and categorized as an excellent antiplasmodial. Molecular docking studies of 3b showed binding interaction with the amino acid residues such as Ala16. Ile 164. Phe58.Tyr170 of the 1J3I.pdb protein and also Ala16, Phe58, Ile 112, Met55 of the 4DP3.pdb protein.Conclusions: An in vitro antimalarial assay of the prepared chalcone derivative(3a-g)showed an excellent and good antiplasmodial activity against the chloroquine-sensitive Pf3D7 strain. In silico antimalarial studies revealed that 3a-g made binding interaction with both sensitive-protein(IJ3I.pdb) and resistance-protein(4DP3.pdb), which means that they were both active against chloroquine-sensitive and resistant plasmodium strain.
文摘In the flavonoid biosynthesis pathway, Chalcone synthase (CHS) is involved in the formation of the pigment and has been shown to be a rate-limiting enzyme for the synthesis of flavonoids. In this study, a PCR approach was used to clone a Chalcone synthases cDNA from flower of sweet osmanthus “Chenghong Dangui” and it was designated as OfCHS (O. fragrans, CHS). The cDNA was 1383 bp long and a coding sequence (CDS) of 1173 bp encoding a polypeptide of 391 amino acids with an estimated molecular mass of 39.9 kDa. The theoretical isoelectric point was 6.23. Phylogenetic analysis demonstrated that OfCHS clustered with Olea europaea, Solenostemon scutellarioides, Perilla frutescens, Antirrhinum majus and Digitalis lanata. We also detected the expression of OfCHS in different tissues in “Dangui” and in two cultivars with varied coloration, “Zi Yingui” and “Chenghong Dangui” at different floral stages using quantitative real-time PCR. We observed that OfCHS transcript was higher in leaves than in petals in “Dangui”. The transcripts of OfCHS in “Zi Yingui” petals were higher than those in “Dangui” at three stages especially at xianyan stage and there was no significant difference between the two cultivars in the full flowering stage. “Chenghong Dangui” has a relatively high anthocyanin content compared to “Zi Yingui”. The relative amount of anthocyanin of “Chenghong Dangui” initially increases, and then decreases during the bloom period. However, the expression of CHS is the highest at the initial flowering stage. These data suggest that the OfCHS does not play a key role in the accumulation of total flavonoid in this cultivar. These data could contribute to explain the different accumulation of flavonoids in petals of the two cultivars.
基金We thank the financial support of Major Program of National Natural Science Foundation of China(21890762)General Program of National Natural Science Foundation of China(21878293)+2 种基金National Natural Science Foundation of China(21676270)Key Research Program of Frontier Sciences,CAS(QYZDY-SSW-JSC011)the K.C.Wong Education Foundation(GJTD-2018-04).
文摘An ionic liquid(IL)catalyzed solvent-free process was developed for the direct synthesis of chalcone and its derivatives by using substituted acetophenones and benzaldehydes via aldol reaction under mild conditions.A series of acidic and basic ILs were selected and screened.The influences of cations and reaction conditions on product yield and selectivity were systematically investigated.The[Bmim]OH was identified as the optimal IL,with the highest yield and selectivity reaching up to 96.7% and 100%,respectively.A reaction mechanism-based kinetic model was established and regressed with experimental data,revealing the β-Hydroxylketone dehydrolysis with activation barrier of 37.8 kJ·mol^(-1) was observed as the ratecontrolling step.
基金supported by the Agricultural Science and Technology Innovation Program, China (ASTIP-TRIC01)
文摘Chalcone synthases (CHS, EC 2.3.1.74) are key enzymes that catalyze the first committed step in flavonoid biosynthesis. In this study, we isolated a chalcone synthase, named NtCHS6, from Nicotiana tabacum. This synthase shared high homology with the NSCHSL (Y14507) gene and contained most of the conserved active sites that are in CHS proteins. The phylogenetic analysis suggested that NtCHS6 protein shared a large genetic distance with other Solanaceae CHS proteins and was the most closely-related to an uncharacterized CHS from Solanum lycopersicum. The expression analysis indicated that NtCHS6 was abundantly expressed in leaves, especially in mature leaves. By scrutinizing its upstream promoter sequences, multiple cis-regulatory elements involved in light and drought responsive were detected. Furthermore, NtCHS6 expression decreased significantly under dark treatment and increased significantly under drought stress suggested that NtCHS6 expression exhibited both light responsiveness and drought responsiveness, and important roles in ultraviolet protection and drought tolerance. Our results might play
基金supported by the National Natural Science Foundation of China,No.81473295(to ZMS),81373312(to XZ)and 81371028(to WCW)
文摘Chalcone is a plant metabolite widely found in fruits,vegetables,spices and tea,and has anti-tumor,anti-inflammation,immunomodulation,antibacterial and anti-oxidation activities,as well as many other pharmacological and biological effects.Our team has shown that its analogs have antioxidant activity,and oxidative stress is a pathological hallmark of retinal ischemia/reperfusion injury that can lead to retinal damage and visual loss.This investigation aims to identify a chalcone that protects retinal ganglion cells in vitro from the effects of oxidative stress and examine its mechanism.Rat retinal ganglion cell-5 cells were pretreated with chalcones and then exposed to tert-butyl hydroperoxide that causes oxidative damage.Controls received dimethyl sulfoxide only or tert-butyl hydroperoxide in dimethyl sulfoxide.Only(E)-3,4-dihydroxy-2′-methylether ketone(L2 H17),of the five chalcone analogs,markedly increased the survival rate of oxidatively injured RGC-5 cells.Thus,subsequent experiments only analyzed the results of the L2 H17 intervention.Cell viability and apoptosis were measured.Intracellular superoxide dismutase and reactive oxygen species levels were used to assess induced oxidative stress.The mechanism of action by L2 H17 was explored by measuring the ER stress/UPR pathway and the expression and localization of Nrf2.All results demonstrated that L2 H17 could reduce the apoptosis of oxidatively injured cells,inhibit caspase-3 activity,increase Bcl-2 expression,decrease Bad expression,increase the activity of superoxide dismutase,inhibit the production of reactive oxygen species,increase Nrf2 immunoreactivity,and reduce the activating transcription factor 4,phospho-eukaryotic initiation factor 2 and CHOP expression.L2 H17 protects retinal ganglion cells induced by oxidative stress by regulating Nrf2,which indicates that it has the potential to become a drug for retinal ischemia/reperfusion.
基金grants from the Advanced Research Projects Agency (MDA-972-9l-J1013, N000l4-90-2032) and the World Health Organization (WHO 940l
文摘In order to accelerate the development of relatively inexpensive antimalarials that are effective against chloroquine-resistant strains of Plasmodium falciparum, a methodology for the solid phase synthesis of chalcone (1, 3-diphenyl-2-propen-1-one) analogues in reasonably high yields has been developed.
文摘Corrosion inhibition of Al and Al-3.5Mg alloy by organic compounds, namely chalcones in hydrochloric acid solutions has been investigated by rapid polarization technique and weight loss method. Polarization measurements show that, the inhibitors act cathodically both in case of Al and Al-3.5Mg alloy. It was found from the weight loss measurements that, the inhibition efficiency depends on the substituent in the chalcone compound. The relative inhibitive efficiency of these compounds has been explained on the basis of structure dependent electron donor properties of the inhibitors and the metal inhibitor interaction on the surface. The inhibition efficiency ranges from 16 to 64% for Al and from 30% to 91% for Al-3.5Mg alloy
文摘Several dimethylamino-substituted chalcone (i .e. dimethylaminobenzal acetophenone) (DBA) derivatives with intramolecular charge transfer transition character were used as visible light sensitizers for radical photopolymerization initiated by iodonium salt (DPIO). Initiating radical species is produced from DBA sensitized photolysis of DPIO through the single electron transfer, accompanying the bleaching of DBA, The activity of DBA decreases as a function of substituent attached to phenyl ring in the order: DBA-2 (OCH_3) >DBA-1(H)> DBA-3 (Cl). The kinetic study on photopolymerization of MMA was carried out in CH_3CN solution at 30 ℃ by dilatometry. The polymerization rate was determined to be proportional to the concentration with exponents of 0.42, 0.25 and 0.86 for DPIO, DBA-1 and MMA, respectively.
文摘A novel chalcone derivative was isolated from Onychium japonicum and its structure was elucidated by spectroscopic methods.The isolated compound showed cytotoxic activity against the human carcinoma cell lines Hela and BEL-7402 in vitro.
文摘Reaction of heterocycle substituted telluronium salts with aroma- tic aldehydes under mild conditions gave corresponding chalcones.In the presence of dibutyl telluride,2-bromomethyl-5-nitrofuran condensed easily with aromatic aldehydes in THF to afford corresponding olefin derivatives as one-pot reaction.
文摘The SHG effects of substituted 3-thienyl chalcone compounds weremeasured. The relationship between structure and effect of substituted 3-thienyl chal-cone derivatives and substituted 2-thienyl chalcone derivatives were discussed. It isshown that their properties of high SHG efficient are similar to each other and the sub-stituted 3-thienyl chalcone molecules are easier to form non-centrosymmetric crystalstructure than substituted 2-thienyl chalcone molecules.
文摘A simple and rapid method was developed using cyclic, differential pulse and square wave voltammetric techniques for the determination of trace-level chalcone at a glassy carbon electrode. Chalcone could produce two anodic peaks at about 0.514 V and 1.478 V and a cathodic peak at about -0.689 V. The differential pulse voltammerty presents a good linear response as compared to square wave voltammetry in the range of 0.2 - 10 μM with a detection limit of 0.18 μM. The proposed method was used successfully for its quantitative determination in spiked human plasma and urine as real samples.
文摘Based on the antitumor activity of chalcone derivatives and their structure, the structure-activity relationship of chalcone derivatives was analyzed by CoMFA (comparative molecular field analysis) method and then the 3D-QSAR (the three dimensional quantitative structures activity relationship) model was established. The analytical results showed that the model had good stability and prediction ability with cross-validated Q2 and non-cross-validated R2 values of 0.527 and 0.995, respectively. The contour map of the model explained the relationship between the structure of chalcone derivatives and antitumor activity, and could be analyzed to design antitumor chalcone derivatives. We designed some structure of chalcone derivatives and calculated their antitumor activity. In this paper, 24 chalcone derivatives were studied by CoMFA, 3D-QSAR, molecular design, which provided theoretical for designing good activities chalcone derivatives.
文摘A systematic conceptual density functional theory (DFT) analysis was performed on a series of chlorinated chalcones to study the effect of electron distribution on antimicrobial activity. In our previous work, a series of 16 chlorinated chalcones were synthesized to determine the antimicrobial effects of varying the location of the halogen substituent on each aromatic ring of the chalcone. Herein is reported a DFT investigation of those 16 chalcones and a comparison of quantum chemical properties to their antimicrobial activity. DFT global chemical reactivity descriptors (chemical hardness/softness, chemical potential/electronegativity, and electrophilicity) and local reactivity descriptors (Fukui functions and dual descriptor) were calculated for all compounds using Spartan’18 software. All calculations were carried out at the B3LYP/6-31G* level of theory. Reactivity analysis of the Fukui dual descriptor calculations reveals sites of nucleophilic and electrophilic attack. These in-silico results provide a foundation for further synthetic optimization of the chalcone skeleton to serve as novel antimicrobial agents.
文摘This paper summarizes the synthesis, growth and the effect of the position of the substituent in the thienyl ring and also the properties of the grown chalcone crystals, 2-CTP and 3-CTP. The formation of compound is confirmed by the re- corded H1NMR spectra. A FT-IR spectrum confirms the presence of all functional groups in both of the crystals. Single crystal XRD reports that even though these two compounds crystallize in monoclinic crystal system, 2-CTP has centro- symmetric P21/c space group and 3-CTP has non-centrosymmetric space group P21. Thermal properties of grown crys- tals analyzed by TG/DTA study explain that the 3-CTP compound is slightly more stable than 2-CTP. The transparency of these isomers in the vis-IR region has been studied. Second and third order nonlinear optical properties of 3-CTP and 2-CTP crystals have been investigated by powder SHG and Z-scan technique respectively.
文摘Background: Chalcones are open-chain flavonoids which display a large number of pharmacological activities such as cytotoxic, anti-inflammatory including antioxidant. The objective of this study was to assess antioxidant and cytotoxic activity of six synthesized chalcones. Methodology: For the current experiments, 1,3-diphenylpropenone (compound R) was used as molecular model to synthetize six compounds, namely three benzyl-benzimidazolyl-chalcones (U1, U2, WAC1) and three imidazopyridinyl-chalcones (V1, V2, V3). All the compounds were evaluated for their ability to scavenge the stable free ABTS.+ radical cation, according to the method develop by Choong et al. In addition, the cytotoxicity test described by Price et al., was performed using healthy human cell line, then in human malignant cell lines (HEP-2, A549). Results: All synthesized chalcones reduced the ABTS.+ radical cation. Indeed, benzyl benzimidazolyl compounds WAC1, U1, U2, by developing respectively 39.61%, 66.09%, and 84.20% percentages of reduction, showed an antioxidant effect 6, 11 and 14 times greater than the compound R (6.14%). As a result, imidazopyridinyl-chalcones compounds, namely V1, V2 and V3 reduced the ABTS.+ radical cation at 91.62%, 99.84% and 97.45% respectively, being 15 and 16 times more active than the compound R. About cytotoxicity, V2 inhibited not significantly HEP-2 malignant cells growth at 48.64%, compared to the standard product, i.e. doxorubicin that inhibited the growth of the same cells at 42.37%. WAC1 inhibited significantly the growth of A549 malignant cells at 89.53%, more than doxorubicin which percentage of growth inhibition was 71.58%. Conclusion: The presence of the α, β-unsaturated carbonyl system (or 1,3-diphenylpropenone) along with a benzimidazole or imidazopyridine heterocyclic ring is likely to contribute to both cytotoxic and antioxidant activities of these compounds.