In this work, the interaction between chelerythrine （CHE） and guanosine is studied using UV-vis and fluorescence measurements at various temperatures. The UV-vis spectra show that the increasing guanosine concentrat...In this work, the interaction between chelerythrine （CHE） and guanosine is studied using UV-vis and fluorescence measurements at various temperatures. The UV-vis spectra show that the increasing guanosine concentrations result in the decreasing absorption intensity and red shift of CHE E absorption band （267 nm）. The fluorescence spectra are fitted to linear analysis, yielding a binding constant of 1.04×10^4 L/tool at 298.15 K of CHE with guanosine. Besides, with △rHm^θ = - 8.26 kJ/mol, △rGm^θ = -22.90 kJ/mol, and △rSm^θ = 49.38 J/（mol K） the interaction should be entropy-driven and exterothermic.展开更多
The binding of chelerythrine （CHE） iminium to calf-thymus （CT） DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polariz...The binding of chelerythrine （CHE） iminium to calf-thymus （CT） DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polarization, and the perturbation of the CD spectra of DNA. These changes are characteristic of intercalation. Scatchard plots constructed from fluorescence titration data gave a binding constant of 5.77×10^5 and a binding site size of 3,4 base pairs at 25 ℃. The intercalative interaction is exothermic with a Van＇t Hoff enthalpy of -33.7 kJ/mol. In addition, △G =-32.9 kJ/mol, and △S =-2.9 J/mol/K. The intercalation of CHE into the DNA helix is enthalpy-driven.展开更多
To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV–vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculat...To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV–vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculations. The binding of CHE to cytidine could be characterized by the hypochromic and bathochromic effects in the absorption bands, and the quenching of fluorescence intensity. The spectral data were fit by linear analysis, yielding a binding constant of 2.49 × 10(4) L mol(?1)at 25 °C of CHE and cytidine, and a van’t Hoff enthalpy of ?20.02 kJ/mol for the exothermic interaction in the standard state. In addition, with and , the interactions should be entropy-driven.展开更多
[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Be...[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Besides,the effects of water quality,light source,oxidant,temperature,and pH on stability were investigated.[Results]The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good;the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable,but they are unstable in tap water whether they are protected from light or not;oxidants have a great influence on the stability of sanguinarine and chelerythrine;sanguinarine and chelerythrine are stable at room temperature lower than 54℃;sanguinarine is stable in pH 2.5-7.0,while chelerythrine is stable in pH 2.5-8.0.[Conclusions]The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.展开更多
Nitidine, Chelerythrine and Sanguinarine, all these three alkaloids are benzophenanthridine alkaloids. Nitidine was used as an anti-HIV, anti-malarial and anti-cancer. Chelerythrine had anti-cancer and anti-inflammato...Nitidine, Chelerythrine and Sanguinarine, all these three alkaloids are benzophenanthridine alkaloids. Nitidine was used as an anti-HIV, anti-malarial and anti-cancer. Chelerythrine had anti-cancer and anti-inflammatory activities. Sanguinarine was widely used as an anti-plaquestic and anti-cancer. High performance thin layer chromatography (HPTLC) method was used for simultaneous quantification of Nitidine, Chelerythrine and Sanguinarine in callus extract of Zanthoxylum rhetsa by using Silica gel 60 F254 as stationary phase and ethyl acetate:methanol:water:diethylamine (30:5:2:0.5 v/v) as mobile phase at 280 nm. The linearity concentration range was 5 - 160 μg/band of each alkaloid. The Rf values of Nitidine, Chelerythrine and Sanguinarine were found to be 0.28, 0.49 and 0.73. The limit of detection and limit of quantification were found to be 0.026, 0.088 μg/spot and 0.010 and 0.033 μg/spot, 0.0104 and 0.035 μg/spot respectively for Nitidine, Chelerythrine and Sanguinarine. HPTLC method was developed and validated according to ICH guidelines for simultaneous estimation of Nitidine, Chelerythrine and Sanguinarine and proved to be simple, specific, accurate, robust and rapid.展开更多
目的 分析降钙素原(PCT)、血清胆碱酯酶(CHE)及C-反应蛋白(CRP)与急性有机磷中毒(AOPP)患者呼吸衰竭的关系。方法 选取太和县人民医院2018年7月至2022年4月收治的AOPP患者102例,根据有无呼吸衰竭发生分为无呼衰组33例(无呼吸衰竭)和呼衰...目的 分析降钙素原(PCT)、血清胆碱酯酶(CHE)及C-反应蛋白(CRP)与急性有机磷中毒(AOPP)患者呼吸衰竭的关系。方法 选取太和县人民医院2018年7月至2022年4月收治的AOPP患者102例,根据有无呼吸衰竭发生分为无呼衰组33例(无呼吸衰竭)和呼衰组69例(呼吸衰竭)。分析两组入院即刻、治疗后1 d、治疗后3 d PCT、CHE及CRP水平,对比不同中毒程度AOPP的PCT、CHE及CRP水平及呼吸衰竭发生率。结果 两组在入院即刻、治疗后1 d、治疗后3 d PCT水平呈上升,且呼衰组在各时间段PCT均高于无呼衰组,差异均有统计学意义(P<0.05)。两组在入院即刻、治疗后1 d、治疗后3 d CHE水平呈上升,且呼衰组在各时间段CHE均低于无呼衰组,差异均有统计学意义(P<0.05)。两组在入院即刻、治疗后1 d、治疗后3 d CRP水平呈上升,且呼衰组各时间段CRP水平高于无呼衰组,差异均有统计学意义(P<0.05)。轻度中毒AOPP患者32例,中度中毒AOPP患者14例,重度中毒AOPP患者56例。AOPP患者PCT、CHE及CRP水平及呼吸衰竭发生率:重度>轻度>中度,差异均有统计学意义(P<0.05)。结论 PCT、CHE及CRP与急性有机磷中毒患者呼吸衰竭存在密切联系,对其后续治疗和预后有一定指导意义。展开更多
基金This project is financed by Jiangxi Boyuan Industry Co.,Ltd.(Jiangxi province,China)
文摘In this work, the interaction between chelerythrine （CHE） and guanosine is studied using UV-vis and fluorescence measurements at various temperatures. The UV-vis spectra show that the increasing guanosine concentrations result in the decreasing absorption intensity and red shift of CHE E absorption band （267 nm）. The fluorescence spectra are fitted to linear analysis, yielding a binding constant of 1.04×10^4 L/tool at 298.15 K of CHE with guanosine. Besides, with △rHm^θ = - 8.26 kJ/mol, △rGm^θ = -22.90 kJ/mol, and △rSm^θ = 49.38 J/（mol K） the interaction should be entropy-driven and exterothermic.
文摘The binding of chelerythrine （CHE） iminium to calf-thymus （CT） DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polarization, and the perturbation of the CD spectra of DNA. These changes are characteristic of intercalation. Scatchard plots constructed from fluorescence titration data gave a binding constant of 5.77×10^5 and a binding site size of 3,4 base pairs at 25 ℃. The intercalative interaction is exothermic with a Van＇t Hoff enthalpy of -33.7 kJ/mol. In addition, △G =-32.9 kJ/mol, and △S =-2.9 J/mol/K. The intercalation of CHE into the DNA helix is enthalpy-driven.
文摘To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV–vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculations. The binding of CHE to cytidine could be characterized by the hypochromic and bathochromic effects in the absorption bands, and the quenching of fluorescence intensity. The spectral data were fit by linear analysis, yielding a binding constant of 2.49 × 10(4) L mol(?1)at 25 °C of CHE and cytidine, and a van’t Hoff enthalpy of ?20.02 kJ/mol for the exothermic interaction in the standard state. In addition, with and , the interactions should be entropy-driven.
基金Supported by Post-doctoral Foundation Project of Heilongjiang Province(LBHZ16094)Special Research Fund Project for Scientific and Technological Innovation Talents in Harbin City(2017RAQXJ115)Doctoral Starting up Project of Harbin University of Commerce(2019DS114).
文摘[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Besides,the effects of water quality,light source,oxidant,temperature,and pH on stability were investigated.[Results]The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good;the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable,but they are unstable in tap water whether they are protected from light or not;oxidants have a great influence on the stability of sanguinarine and chelerythrine;sanguinarine and chelerythrine are stable at room temperature lower than 54℃;sanguinarine is stable in pH 2.5-7.0,while chelerythrine is stable in pH 2.5-8.0.[Conclusions]The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.
文摘Nitidine, Chelerythrine and Sanguinarine, all these three alkaloids are benzophenanthridine alkaloids. Nitidine was used as an anti-HIV, anti-malarial and anti-cancer. Chelerythrine had anti-cancer and anti-inflammatory activities. Sanguinarine was widely used as an anti-plaquestic and anti-cancer. High performance thin layer chromatography (HPTLC) method was used for simultaneous quantification of Nitidine, Chelerythrine and Sanguinarine in callus extract of Zanthoxylum rhetsa by using Silica gel 60 F254 as stationary phase and ethyl acetate:methanol:water:diethylamine (30:5:2:0.5 v/v) as mobile phase at 280 nm. The linearity concentration range was 5 - 160 μg/band of each alkaloid. The Rf values of Nitidine, Chelerythrine and Sanguinarine were found to be 0.28, 0.49 and 0.73. The limit of detection and limit of quantification were found to be 0.026, 0.088 μg/spot and 0.010 and 0.033 μg/spot, 0.0104 and 0.035 μg/spot respectively for Nitidine, Chelerythrine and Sanguinarine. HPTLC method was developed and validated according to ICH guidelines for simultaneous estimation of Nitidine, Chelerythrine and Sanguinarine and proved to be simple, specific, accurate, robust and rapid.
文摘目的 分析降钙素原(PCT)、血清胆碱酯酶(CHE)及C-反应蛋白(CRP)与急性有机磷中毒(AOPP)患者呼吸衰竭的关系。方法 选取太和县人民医院2018年7月至2022年4月收治的AOPP患者102例,根据有无呼吸衰竭发生分为无呼衰组33例(无呼吸衰竭)和呼衰组69例(呼吸衰竭)。分析两组入院即刻、治疗后1 d、治疗后3 d PCT、CHE及CRP水平,对比不同中毒程度AOPP的PCT、CHE及CRP水平及呼吸衰竭发生率。结果 两组在入院即刻、治疗后1 d、治疗后3 d PCT水平呈上升,且呼衰组在各时间段PCT均高于无呼衰组,差异均有统计学意义(P<0.05)。两组在入院即刻、治疗后1 d、治疗后3 d CHE水平呈上升,且呼衰组在各时间段CHE均低于无呼衰组,差异均有统计学意义(P<0.05)。两组在入院即刻、治疗后1 d、治疗后3 d CRP水平呈上升,且呼衰组各时间段CRP水平高于无呼衰组,差异均有统计学意义(P<0.05)。轻度中毒AOPP患者32例,中度中毒AOPP患者14例,重度中毒AOPP患者56例。AOPP患者PCT、CHE及CRP水平及呼吸衰竭发生率:重度>轻度>中度,差异均有统计学意义(P<0.05)。结论 PCT、CHE及CRP与急性有机磷中毒患者呼吸衰竭存在密切联系,对其后续治疗和预后有一定指导意义。
