期刊文献+
共找到6篇文章
< 1 >
每页显示 20 50 100
Deep chemical identification of phytoecdysteroids in Achyranthes bidentata Blume by UHPLC coupled with linear ion trap-Orbitrap mass spectrometry and targeted isolation
1
作者 WANG Ying-Ying LI Jia-Yuan +8 位作者 YAO Chang-Liang ZHANG Jian-Qing YU Yang YAO Shuai GAO Min WU Shi-Fei WEI Wen-Long BI Qi-Rui GUO De-An 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2022年第7期551-560,共10页
Achyranthes bidentata Blume is widely used as a traditional Chinese medicine with the effects of nourishing the liver and kidneys and strengthening muscles and bones.In this work,a rapid and simple strategy was develo... Achyranthes bidentata Blume is widely used as a traditional Chinese medicine with the effects of nourishing the liver and kidneys and strengthening muscles and bones.In this work,a rapid and simple strategy was developed for characterizing phytoecdysteroids by ultra-high-performance liquid chromatography coupled with liner ion trap-Orbitrap mass spectrometry using electrospray ionization in the negative mode.As a result,47 phytoecdysteroids were unambiguously or tentatively characterized.Among them,seven known compounds were identified according to the reference standards along with molecular formula,retention time and fragmentation patterns,while others were mostly potential new compounds.Through targeted isolation,the structures of three new compounds were determined by NMR spectra,which were consistent with LC-MS characterization.The present study provides an efficient method to deeply characterize phytoecdysteroids. 展开更多
关键词 Achyranthes bidentata Blume chemical identification Fragmentation behaviors PHYTOECDYSTEROIDS Targeted isola-tion
原文传递
A Report on Identification, Segregation, Disposal and Reduction of Chemical Wastes
2
作者 Joanna V. Toralba Joanna J. Orejola Myra Fe S. Tacadao Karen C. Tan Levi Letlet H. Larcia II Cherry Caroline S. Chua Raquel C. Jadulco-Koch 《Journal of Chemistry and Chemical Engineering》 2011年第9期783-792,共10页
The accumulation of generated chemical wastes posed a serious problem for the UP College of Pharmacy. The faculty from the Department of Pharmaceutical Chemistry devised a scheme for the identification and segregation... The accumulation of generated chemical wastes posed a serious problem for the UP College of Pharmacy. The faculty from the Department of Pharmaceutical Chemistry devised a scheme for the identification and segregation of the chemical wastes and created a system for proper disposal of future chemical wastes. There were a total of 1,142 of accumulated chemical waste bottles, 804 of which were subjected to identification and segregation procedures. The wastes were preliminarily tested for its solubility/miscibility in water and acidity or basicity. The identification was done by qualitative tests for cyanide, sulfide, halogenated, non-halogenated, oxidizing, nitro, and heavy metal compounds. The final segregation was based on the presence of the most hazardous component or on pH and water-miscibility. The Department then developed and implemented a scheme for the proper disposal of the chemical wastes generated in laboratory experiments done in the College. Laboratory experiments were also modified to use less toxic and less amounts of chemicals. 展开更多
关键词 chemical waste identification chemical waste treatment chemical waste disposal chemical wastes.
下载PDF
Systematic Evaluation of Pharmacognostic Identification of Polygonum capitatum 被引量:1
3
作者 Bo TU Xu ZHANG +3 位作者 Minghui HE Shanggao LIAO Yongqin ZENG Yan LIN 《Medicinal Plant》 CAS 2023年第4期9-13,共5页
[Objectives] To investigate the systematic evaluation of pharmacognostic identification of Polygonum capitatum . [Methods] 10 batches of P. capitatum cultivated in Guizhou were chosen for plant samples. Macroscopical ... [Objectives] To investigate the systematic evaluation of pharmacognostic identification of Polygonum capitatum . [Methods] 10 batches of P. capitatum cultivated in Guizhou were chosen for plant samples. Macroscopical identification was conducted on plant roots, stems, leaves, flowers and fruits. The P. capitatum powder was processed for physical and chemical distinction by FeCl 3 chromogenic reaction, hydrochloric acid magnesium powder reaction, AlCl 3 color development reaction and thin-layer chromatography.Microscope identification was carried out on the powder. Plant genome DNeasy Plant Kit was adopted for DNA molecular marker identification. [Results] The results showed that the stem of P. capitatum was tufted, the leaves were oval, 2 to 5 cm long, and 1 to 2 cm wide;the leaf apex was acute and cuneate at the base, the inflorescence was capitate, paired or solitary;the raceme was erect and nearly spherical, and the perianth was light red. Furthermore, for the chromogenic reaction of FeCl 3 ethanol extract of P. capitatum , appeared blue and turned to dark blue after long time storing at room temperature. For the reaction of hydrochloric acid magnesium powder, the alcohol extract of P. capitatum , exhibited deep red. In the color reaction of AlCl 3, the alcohol extract revealed yellow fluorescence under 360 nm UV lamp. Microscope identification of the powder displayed pollen grains, crystal sheath fibers, cellulose, vessels, starch grains, cork cells, and other characteristic fragments. In addition, DNA barcoding electrophoresis results showed that P. capitatum showed a clear and bright single band near 500 bp, and further sequencing results showed that the sequence differences were mainly concentrated in ITS1 and ITS2 region. [Conclusions] Systematic evaluation for the identification of P. capitatum is established, which combines with macroscopic identification, physicochemical identification, powder microscope identification, and DNA molecular identification. Finally, the original medicinal material is identified as P. capitatum Buch.-Ham. ex D. Don. 展开更多
关键词 Chinese herbal medicine Polygonum capitatum Pharmacognostic identification Character identification Physical and chemical identification Microscopic identification DNA molecular marker identification
下载PDF
Separation and Preliminary Identification of Intestinal Probiotics of Laying Hens
4
作者 Guo Shijin Zhang Zhimei +4 位作者 Fu Shijun Mo Ling Zhang Ying Shen Zhiqiang Huang Baohua 《Animal Husbandry and Feed Science》 CAS 2018年第1期72-74,共3页
[Objective] The paper was to isolate and identify probiotics in the intestine of laying hens. [Method] The intestinal probiotics in laying hens at peak period were isolated using conventional separation methods; the p... [Objective] The paper was to isolate and identify probiotics in the intestine of laying hens. [Method] The intestinal probiotics in laying hens at peak period were isolated using conventional separation methods; the physical and chemical properties of target strains and in vitro antibacterial effects were measured. Moreover, the safety test of chicks was conducted. [Result] Four strains of lactobacillus were isolated from the jejunum of laying hens, including Lactobacillus plantarum, Bacillus acidophilus, L. delbrueckii subsp. Delbrueckii and L. delbrueckii subsp. Lactis, and their inhibition zone diameters were 18.30, 16.07, 11.45, 17.26 mm, respectively. One strain of Lactobacillus, L. brevis, was isolated from the cecum, with the inhibition zone diameter of 10.26 mm. Three strains of bacillus were isolated from the cecum, including Bacillus subtilis, B. cereus and B. licheniformis, and their inhibition zone diameters were 9.25, 8.46 and 8.37 mm, respectively. Daily drinking 2 billion units of viable bacteria was the safe dosage for chicks. [Conclusion] Eight strains of probiotics had certain inhibitory effect on Escherichia coli O;, and had no toxic side effects to chicks. Lactobacillus had strong antibacterial effect on E. coli O;, while the antibacterial effect of bacillus was relatively weak. 展开更多
关键词 Laying hen Intestine Probiotics Isolation identification Physical and chemical properties Antibacterial Safety test
下载PDF
Study on HPLC-DAD Fingerprint of Xueshuan Xinmaining Capsules
5
作者 Chuang ZHAO Yuling LIU +3 位作者 Longfei LIN Chunmin WANG Hui LI Yujie YANG 《Medicinal Plant》 2024年第5期31-34,49,共5页
[Objectives]To establish the HPLC-DAD fingerprint of Xueshuan Xinmaining Capsule(XXC).[Methods]The chromatographic conditions for the analysis of XXC solution were as follows:XSelect HSS T3 column;acetonitrile-0.1%pho... [Objectives]To establish the HPLC-DAD fingerprint of Xueshuan Xinmaining Capsule(XXC).[Methods]The chromatographic conditions for the analysis of XXC solution were as follows:XSelect HSS T3 column;acetonitrile-0.1%phosphoric acid water was used as mo-bile phase,gradient elution;flow rate:1.0 mL/min;column temperature 30℃;The injection volume is 10μL.The quality of XXC samples produced by different manufacturers was evaluated by similarity evaluation and cluster analysis.[Results]In theHPLC-dad fingerprints of 15 batches of XXC,23 common peaks were identified and 9 peaks were identified,and the similarity was greater than 0.95.According to the re-sults of cluster analysis,15 batches of XXC samples could be divided into two categories,S2,S5,S6,S7 and S8 batches belonged to category Ⅰ,and the rest batches belonged to category Ⅱ.[Conclusions]In this study,a representative and universal identification method of Xxc HPLC-DAD fingerprint was established.The method has high precision,stability and repeatability,is simple and reliable,and provides a pow-erful reference for further improving the quality evaluation system of XXC. 展开更多
关键词 Xueshuan Xinmaining Capsule(XXC) HPLC-DAD FINGERPRINT identification of chemical component Quality evaluation
下载PDF
Identification and Preliminary Characterization of a New Chemical Affecting Glucosyltransferase Activities Involved in Plant Cell Wall Biosynthesis 被引量:1
6
作者 Olga Zabotina Erik Maim +2 位作者 Georgia Drakakaki Vincent Bulone Natasha Raikhel 《Molecular Plant》 SCIE CAS CSCD 北大核心 2008年第6期977-989,共13页
Chemical genetics as a part of chemical genomics is a powerful and fast developing approach to dissect biological processes that may be difficult to characterize using conventional genetics because of gene redundancy ... Chemical genetics as a part of chemical genomics is a powerful and fast developing approach to dissect biological processes that may be difficult to characterize using conventional genetics because of gene redundancy or lethality and, in the case of polysaccharide biosynthesis, plant flexibility. Polysaccharide synthetic enzymes are located in two main compartments--the Golgi apparatus and plasma membrane-and can be studied in vitro using membrane fractions. Here, we first developed a high-throughput assay that allowed the screening of a library of chemicals with a potential effect on glycosyltransferase activities. Out of the 4800 chemicals screened for their effect on Golgi glucosyltransferases, 66 compounds from the primary screen had an effect on carbohydrate biosynthesis. Ten of these compounds were confirmed to inhibit glucose incorporation after a second screen. One compound exhibiting a strong inhibition effect (ID 6240780 named chemical A) was selected and further studied. It reversibly inhibits the transfer of glucose from UDP-glucose by Golgi membranes, but activates the plasma membrane-bound callose synthase. The inhibition effect is dependent on the chemical structure of the compound, which does not affect endomembrane morphology of the plant cells, but causes changes in cell wall composition. Chemical A represents a novel drug with a great potential for the study of the mechanisms of Golgi and plasma membrane-bound glucosyltransferases. 展开更多
关键词 cell walls callose synthase chemical screening and identification GLYCOSYLTRANSFERASE GOLGI plasma membrane
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部