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Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk 被引量:5
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作者 LUO Peng Jie ZHANG Jian Bo +8 位作者 WANG Hua Li CHEN Xia WU Nan ZHAO Yun Feng WANG Xiao Mei ZHANG Hong ZHANG Ji Yue ZHU Lei JIANG Wen Xiao 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2016年第5期374-378,共5页
Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIE... Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC50 value was 2.4 ng/mL in buffer. 展开更多
关键词 CLEIA Rapid and Sensitive chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk
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Determination of theophylline concentration in serum by chemiluminescent immunoassay 被引量:2
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作者 周美霞 管茶英 +2 位作者 陈光 谢鑫友 吴盛海 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第12期1148-1152,共5页
Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and eval... Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay.Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 μmol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study oftheophylline. 展开更多
关键词 THEOPHYLLINE chemiluminescent immunoassay (CLIA) Fluorescence polarization immunoassay (FPIA)
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Determination of Progesterone Receptor by Chemiluminescent Enzyme Immunoassay
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作者 殷铁军 顾美皎 +3 位作者 周宜开 郑文 胡伟 芦运萍 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2003年第1期60-61,93,共3页
A new method of chemiluminescent enzyme immunoassay (CLEIA) was developed and the standard curve and regression equation for determination of progesterone receptor (PR) made. The luminosity of tissue samples was teste... A new method of chemiluminescent enzyme immunoassay (CLEIA) was developed and the standard curve and regression equation for determination of progesterone receptor (PR) made. The luminosity of tissue samples was tested and PR level was calculated by the regression equation. Correlation analysis revealed that there was a linear relationship between different concentrations of the standard PR samples and the corresponding values of luminosity: Y=3748+463.77X, γ=0 9958. The values of the luminosity in 38 cases of tumor tissues were determined with the highest being 267.32 fmol/mg, the lowest 3.69 fmol/mg and the mean 78.53 fmol/mg. The new method of CLEIA was a stable, creditable,specific and sensitive assay for determination of PR. 展开更多
关键词 progesterone receptor chemiluminescent enzyme immunoassay
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Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein 被引量:5
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作者 Qian-Yun Zhang a,b,Hui Chen a,Zhen Lin a,Jin-Ming Lin a a Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Department of Chemistry,Tsinghua University,Beijing 100029,China b Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China 《Journal of Pharmaceutical Analysis》 SCIE CAS 2012年第2期130-135,共6页
A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum a-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELI... A chemiluminescence enzyme immunoassay based on magnetic microparticles (MmPs-CLEIA) was developed to evaluate serum a-fetoprotein (AFP) in parallel with traditional colorimetric enzyme-linked immunosorbent assay (ELISA).A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents,less total assay time,and better linearity,recovery,precision,sensitivity and validity.AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA,and the results were compared with commercial electrochemiluminescence immunoassay (ECLIA) kit.The correlation coefficient between MPs-CLEIA and ELISA was obtained with R 2 0.6703;however,the correlation between MPs-CLEIA and ECLIA (R 2 0.9582) was obviously better than that between colorimetric ELISA and ECLIA (R 2 0.6866). 展开更多
关键词 a-Fetoprotein Hepatocellular carcinoma Chemiluminescence enzyme immunoassay Magnetic microparticles Colorimetric enzyme-linked immunosorbent assay
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Summary of the Detection Kits for SARS-CoV-2 Approved by the National Medical Products Administration of China and Their Application for Diagnosis of COVID-19 被引量:6
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作者 Ruhan A Huijuan Wang +1 位作者 Wenling Wang Wenjie Tan 《Virologica Sinica》 SCIE CAS CSCD 2020年第6期699-712,共14页
The on-going global pandemic of coronavirus disease 2019(COVID-19)caused by a novel coronavirus called severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has been underway for about 11 months.Through November ... The on-going global pandemic of coronavirus disease 2019(COVID-19)caused by a novel coronavirus called severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has been underway for about 11 months.Through November 20,2020,51 detection kits for SARS-CoV-2 nucleic acids(24 kits),antibodies(25 kits),or antigens(2 kits)have been approved by the National Medical Products Administration of China(NMPA).Convenient and reliable SARS-CoV-2 detection assays are urgently needed worldwide for strategic control of the pandemic.In this review,the detection kits approved in China are summarised and the three types of tests,namely nucleic acid,serological and antigen detection,which are available for the detection of COVID-19 are discussed in detail.The development of novel detection kits will lay the foundation for the control and prevention of the COVID-19 pandemic globally. 展开更多
关键词 Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) Detection kits Real-time RT-PCR Lateral flow immunoassay(LFIA) Chemiluminescence immunoassay(CLIA)
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Serologic Response to SARS-CoV-2 in COVID-19 Patients with Different Severity 被引量:5
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作者 Wen-Hua Kong Rong Zhao +5 位作者 Jun-Bo Zhou Fang Wang De-Guang Kong Jian-Bin Sun Qiong-Fang Ruan Man-Qing Liu 《Virologica Sinica》 SCIE CAS CSCD 2020年第6期752-757,共6页
The immense patient number caused by coronavirus disease 2019(COVID-19)global pandemic brings the urge for more knowledge about its immunological features,including the profile of basic immune parameters.In this study... The immense patient number caused by coronavirus disease 2019(COVID-19)global pandemic brings the urge for more knowledge about its immunological features,including the profile of basic immune parameters.In this study,eighty-eight reported COVID-19 patients in Wuhan were recruited from January to February,2020,including 32 severe/critical cases and56 mild/moderate cases.Their mean age was 56.43 years(range 17–83)and gender ratio(male/female)was 43:45.We tested SARS-CoV-2 RNA with commercial kits,investigated the level of serologic IgM and IgG antibodies against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)using magnetic particle chemiluminescence immunoassays,and compared the results of serologic tests and nucleic acid test(NAT).Among 88 patients,95.45%were confirmed as positive by the combination of NAT and antibody test,which was significantly higher(P<0.001)than by single nucleic acid test(73.86%)or serologic test(65.91%).Then the correlation between temporal profile and the level of antibody response was analyzed.It showed that seroconversion started on day 5 after disease onset and IgG level was rose earlier than IgM.Comparison between patients with different disease severity suggested early seroconversion and high antibody titer were linked with less severe clinical symptoms.These results supported the combination of serologic testing and NAT in routine COVID-19 diagnosis and provided evidence on the temporal profile of antibody response in patients with different disease severity. 展开更多
关键词 COVID-19 Nucleic acid test(NAT) Serologic test Real-time polymerase chain reaction(RT-PCT) Chemiluminescence immunoassay(CLIA)
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Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin Ⅰ in whole blood samples 被引量:2
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作者 Huan Zhao Enben Su +7 位作者 Li Huang Yunfeng Zai Yuan Liu Zhu Chen Song Li Lian Jin Yan Deng Nongyue He 《Chinese Chemical Letters》 SCIE CAS CSCD 2022年第2期743-746,共4页
Chemiluminescence immunoassay(CLⅠA) has always been a great challenge in detecting cardiac troponin Ⅰ(c Tn Ⅰ) in whole blood samples without centrifugation because of the interference of red blood cells and low sen... Chemiluminescence immunoassay(CLⅠA) has always been a great challenge in detecting cardiac troponin Ⅰ(c Tn Ⅰ) in whole blood samples without centrifugation because of the interference of red blood cells and low sensitivity. Ⅰn this study, the antigens and erythrocytes in the blood were captured by the antibodies immobilized on the magnetic particles, recognized by another biotinconjugated c Tn Ⅰ antibody and detected by streptavidin/acridine aster-conjugated polychloromethylstyrene microspheres(PCMS). After magnetic separation, the supernatant was transferred and measured. No significant difference was noted between the c Tn Ⅰ concentrations of the serum samples,plasma samples and whole blood. The prepared PCMS provided more functional areas to conjugate streptavidin and acridinium ester, so the immunoassay has highly sensitive, the limits of blank at0.012 ng/mL, and functional sensitivity at 0.019 ng/mL with a CV of 20%, and 0.058 ng/mL with a CV of 10%. Total precision of any sample type ranged from 2.62%~5.67%. The assay was linear over the studied range of 0.01-50.00 ng/mL, and no hook effect was found when c Tn Ⅰ concentrations reached 1900 ng/mL. No significant interference was noted with the potential endogenous interfering substances. Compared with the commercial kit(Abbott assay kit), the correlation coefficient was 0.9859. A washing-free CLⅠA was established for the rapid detection of c Tn Ⅰ in human whole blood, using erythrocyte capture antibodies-conjugated magnetic nanoparticles for eliminating the influence of erythrocytes and PCMS for signal amplification, which showed great potential in clinical application. 展开更多
关键词 Washing-free Whole blood Chemiluminescence immunoassay Polychloromethylstyrene microspheres
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N-(β-Carboxypropionyl)luminol as a new chemiluminescence label in immunoassay
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作者 ZHUANG,Hui-Sheng WANG,Qiong-E ZHANG,FanDepartment of Chemistry,Fuzhou University,Fuzhou,Fujian 350002,China.HUANG,Qiao-Jia TANG,Yu-ChaiFuzhou General Hospital,Nanjing Military Region,Fuzhou,Pujian 350002,China 《Chinese Journal of Chemistry》 SCIE CAS CSCD 1997年第2期123-129,共7页
A new chemilurninescence label N-(β-carboxypropionyl)luminol (CPL) was used to label sheep anti-human IgG (SaHIgG).The labeled antibody was stable and could be detected at least down to 10-17~10-16 mol.The molar inco... A new chemilurninescence label N-(β-carboxypropionyl)luminol (CPL) was used to label sheep anti-human IgG (SaHIgG).The labeled antibody was stable and could be detected at least down to 10-17~10-16 mol.The molar incorporation ratio was estimated to be 0.26 mol of CPL per mol of SaHIgG.There were no apparent changes in the immunoreactivity of the labeled SaHIgG and in the quantum efficiency of the CPL after labeling. 展开更多
关键词 Chemiluminescence immunoassay N-(β-carbaxypropionyl)luminol sheep anti-human IgG
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