Chestnut blight caused by Cryphonectria parasitica is one of the major chestnut diseases occurred on stems and branches. Oriental chestnut has more resistance than American one. Twenty-five cultivars of chestnut treei...Chestnut blight caused by Cryphonectria parasitica is one of the major chestnut diseases occurred on stems and branches. Oriental chestnut has more resistance than American one. Twenty-five cultivars of chestnut treeincluding various cultivars such as Tsukuba, Okkwang, Rihei, Ginyose, Eunsan, etc. were tested with a rapid, simple and reliable method. The cultivar Banseki is one of the most resistant cultivars tested and has the necrotic area of 0.40 cm2. However, the necrotic areas of the cultivars of American chestnut, Eunsan, Kwangjujoyul and Eaton, ranges from 3.35 to 2.61 cm2 and these cultivars are relatively susceptible to this pathogen. Many pycnidia of this pathogen were observed on the inner and outer bark tissues after incubating for 3 weeks.展开更多
用酿酒酵母(Saccharyomyces cerevisae)泛素融合蛋白基因从COGEME(Consortium for the functional ge-nomics of microbial eukaryotes)植物病原菌EST(Expressed sequence tag)库中BLAST(Basic local alignment searchtool)得到栗疫病...用酿酒酵母(Saccharyomyces cerevisae)泛素融合蛋白基因从COGEME(Consortium for the functional ge-nomics of microbial eukaryotes)植物病原菌EST(Expressed sequence tag)库中BLAST(Basic local alignment searchtool)得到栗疫病菌的泛素融合蛋白EST,设计引物从栗疫病菌cDNA中克隆到该基因并测序,得到在进化上高度保守的基因,该基因开放阅读框为387 bp,编码128个氨基酸残基组成的泛素融合蛋白前体;由cDNA序列推定的氨基酸序列分析结果表明,泛素融合蛋白前体包括氮末端的泛素结构域(76个氨基酸残基)和碳末端的核糖体蛋白L40结构域(52个氨基酸残基)。该蛋白为高碱性蛋白,碳末端含有一个"锌指"模式结构。与19个物种比较的结果表明,栗疫病菌与真菌泛素融合蛋白氨基酸序列相似度较高,具有高度的保守性。展开更多
The results showed that the contents of free serine\,glutamate\,proline\,alanine\,cystine\,valine\,isoleucine and total amino acid in the resistant varieties after inoculation during incubation period gradually decrea...The results showed that the contents of free serine\,glutamate\,proline\,alanine\,cystine\,valine\,isoleucine and total amino acid in the resistant varieties after inoculation during incubation period gradually decreased and reached the lowest point at 168 h after inoculation,the contents of the free aspartate\,threonine\,serine\,glycine\,alanine\,cystine\,valine\,isoleucine\,leucine\,phenylalanine and total amino acid in the middle resistant varieties gradually decreased and reached the lowest point at 168 h,the content of free glutamate in the middle susceptible varieties after inoculation during incubation period gradually increased and reached the highest point at 168 h,other kinds of amino acid in the middle susceptible varieties didn′t show regular changes,the content of free proline in the susceptible varieties after inoculation during incubation period gradually increased and the other kinds of amino acid didn′t show regular changes.Before inoculation the contents of total free amino acid in different resistant varieties didn′t show regular changes,but those of free alanine and cystine were positively relative to the susceptibility of the host,and that of the free phenylalanine were positively relative to the resistance of the variety.At 168h after inoculation the contents of free proline\,glutamate\,serine\,glycine\,alanine and cystine were negatively relative to the resistance of variety.展开更多
文摘Chestnut blight caused by Cryphonectria parasitica is one of the major chestnut diseases occurred on stems and branches. Oriental chestnut has more resistance than American one. Twenty-five cultivars of chestnut treeincluding various cultivars such as Tsukuba, Okkwang, Rihei, Ginyose, Eunsan, etc. were tested with a rapid, simple and reliable method. The cultivar Banseki is one of the most resistant cultivars tested and has the necrotic area of 0.40 cm2. However, the necrotic areas of the cultivars of American chestnut, Eunsan, Kwangjujoyul and Eaton, ranges from 3.35 to 2.61 cm2 and these cultivars are relatively susceptible to this pathogen. Many pycnidia of this pathogen were observed on the inner and outer bark tissues after incubating for 3 weeks.
文摘用酿酒酵母(Saccharyomyces cerevisae)泛素融合蛋白基因从COGEME(Consortium for the functional ge-nomics of microbial eukaryotes)植物病原菌EST(Expressed sequence tag)库中BLAST(Basic local alignment searchtool)得到栗疫病菌的泛素融合蛋白EST,设计引物从栗疫病菌cDNA中克隆到该基因并测序,得到在进化上高度保守的基因,该基因开放阅读框为387 bp,编码128个氨基酸残基组成的泛素融合蛋白前体;由cDNA序列推定的氨基酸序列分析结果表明,泛素融合蛋白前体包括氮末端的泛素结构域(76个氨基酸残基)和碳末端的核糖体蛋白L40结构域(52个氨基酸残基)。该蛋白为高碱性蛋白,碳末端含有一个"锌指"模式结构。与19个物种比较的结果表明,栗疫病菌与真菌泛素融合蛋白氨基酸序列相似度较高,具有高度的保守性。
文摘The results showed that the contents of free serine\,glutamate\,proline\,alanine\,cystine\,valine\,isoleucine and total amino acid in the resistant varieties after inoculation during incubation period gradually decreased and reached the lowest point at 168 h after inoculation,the contents of the free aspartate\,threonine\,serine\,glycine\,alanine\,cystine\,valine\,isoleucine\,leucine\,phenylalanine and total amino acid in the middle resistant varieties gradually decreased and reached the lowest point at 168 h,the content of free glutamate in the middle susceptible varieties after inoculation during incubation period gradually increased and reached the highest point at 168 h,other kinds of amino acid in the middle susceptible varieties didn′t show regular changes,the content of free proline in the susceptible varieties after inoculation during incubation period gradually increased and the other kinds of amino acid didn′t show regular changes.Before inoculation the contents of total free amino acid in different resistant varieties didn′t show regular changes,but those of free alanine and cystine were positively relative to the susceptibility of the host,and that of the free phenylalanine were positively relative to the resistance of the variety.At 168h after inoculation the contents of free proline\,glutamate\,serine\,glycine\,alanine and cystine were negatively relative to the resistance of variety.
