For sweet cherry, the economic benefit of protected cultivation is much higher than that of open field cultivation. However, problems such as nonuniform germination, aberrant flower, abortion, bad fruit setting and de...For sweet cherry, the economic benefit of protected cultivation is much higher than that of open field cultivation. However, problems such as nonuniform germination, aberrant flower, abortion, bad fruit setting and delayed maturation appear frequently due to the inaccurate regulation of chilling requirement. In order to solve the problems, an automatic system for monitoring, selecting and recording of chilling requirement in protected cultivation of sweet cherry was designed and developed. The system, which consisted of temperature detection, effective chilling temperature selection, signal conversion and output and chilling accumulation display, had been continuously used in protected cultivation of sweet cherry for 3 years and good effects had been achieved. The system monitored chilling accumu- lation accurately and made it more reasonable for the time selection of greenhouse covering, thus avoiding risks of nonuniform germination, bad fruit setting and de- layed maturation, etc. Under the coordinate application of this system with other modern measuring and controlling techniques such as automatic detection and alarming of temperature and humidity, the environment in greenhouses was regulated precisely that the uniform fruits were obtained and the expected yield, quality and harvest time were achieved.展开更多
Determination of chilling and heat requirements of pistachio （Pistacia vera L.） cultivars is important for satisfactory growth and development, particularly when large-scale commercial production is desired. This ex...Determination of chilling and heat requirements of pistachio （Pistacia vera L.） cultivars is important for satisfactory growth and development, particularly when large-scale commercial production is desired. This experiment was conducted to determine chilling requirement inducing vegetative and flower buds of Kalle-Ghuchi, Owhadi, Ahmad-Ahgaei, and Akbari pistachio. Shoots with enough vegetative and flower buds were taken from pistachio trees during autumn 2007 and 2008 when temperature reached to 15°C. Cuttings with flower buds were kept under （5 ± 1）°C for 0, 600, 650, 700, 750, 800, 850, 900, 950, 1 000, 1 050, 1 100, 1 150, 1 200, 1 250, and 1 300 h, respectively, and cuttings with vegetative buds were kept at 51°C until 1 500 h. The results indicated that pistachio cultivars requite chilling time between 750-1 400 h and heat requirements between 8 852-15 420 growing degree hours （GDH）. Consequently, Kalle-Ghuchi had the lowest chilling （750-950 h）, and heat （8 852-9 768 GDH） requirements, Ahmad-Aghaei and Owhadi had intermediate （1 000-1 250 h, 10 656- 13 320 GDH） and Akbari had the highest chilling （1 200-1 400 h） and heat （11 863-15 420 GDH） requirement.展开更多
Bud dormancy facilitates the survival of meristems under harsh environmental conditions.To elucidate how molecular responses to chilling accumulation controlling dormancy in peach buds,chromatin immunoprecipitation se...Bud dormancy facilitates the survival of meristems under harsh environmental conditions.To elucidate how molecular responses to chilling accumulation controlling dormancy in peach buds,chromatin immunoprecipitation sequencing to identify the H3K27me3 modifications and RNA sequencing of two peach cultivars with pronounced differences in chilling requirement were carried out,the results showed that genes associated with abscisic acid and gibberellic acid signal pathways play key roles in dormancy regulation.The results demonstrated that peach flower bud differentiation occurred continuously in both cultivars during chilling accumulation,which was correlated with the transcript abundance of key genes involved in phytohormone metabolism and flower bud development under adverse conditions.The more increased strength in high chillingrequirement cultivar along with the chilling accumulation at the genome-wide level.The function of the dormancy-associated MADS-box gene PpDAM6 was identified,which is involved in leaf bud break in peach and flower development in transgenic Nicotiana tabacum(NC89).In addition,PpDAM6 was positively regulated by PpCBF,and the genes of putative dormancy-related and associated with metabolic pathways were proposed.Taken together,these results constituted a theoretical basis for elucidating the regulation of peach bud dormancy transition.展开更多
基金Supported by"New variety breeding of Sweet Cherry"belongs to National Science-technology Support Plan Projects for the 12th Five-Year Plan(2013BAD02B03-3-2)"948"Project of Agricultural Department(2011-Z40)+2 种基金Special Fund for Fruit Innovation Team of Modern Agricultural Industry Technology System in Shandong Province(SDAIT-03-022-04)Shandong Agricultural Engineering Project of Improved Variety(2013)"Introduction of Excellent Fruit Germplasm Resource and Germplasm Bank"belongs to National Science and Technology Cooperation Projects(2012DFR30700)
文摘For sweet cherry, the economic benefit of protected cultivation is much higher than that of open field cultivation. However, problems such as nonuniform germination, aberrant flower, abortion, bad fruit setting and delayed maturation appear frequently due to the inaccurate regulation of chilling requirement. In order to solve the problems, an automatic system for monitoring, selecting and recording of chilling requirement in protected cultivation of sweet cherry was designed and developed. The system, which consisted of temperature detection, effective chilling temperature selection, signal conversion and output and chilling accumulation display, had been continuously used in protected cultivation of sweet cherry for 3 years and good effects had been achieved. The system monitored chilling accumu- lation accurately and made it more reasonable for the time selection of greenhouse covering, thus avoiding risks of nonuniform germination, bad fruit setting and de- layed maturation, etc. Under the coordinate application of this system with other modern measuring and controlling techniques such as automatic detection and alarming of temperature and humidity, the environment in greenhouses was regulated precisely that the uniform fruits were obtained and the expected yield, quality and harvest time were achieved.
文摘Determination of chilling and heat requirements of pistachio （Pistacia vera L.） cultivars is important for satisfactory growth and development, particularly when large-scale commercial production is desired. This experiment was conducted to determine chilling requirement inducing vegetative and flower buds of Kalle-Ghuchi, Owhadi, Ahmad-Ahgaei, and Akbari pistachio. Shoots with enough vegetative and flower buds were taken from pistachio trees during autumn 2007 and 2008 when temperature reached to 15°C. Cuttings with flower buds were kept under （5 ± 1）°C for 0, 600, 650, 700, 750, 800, 850, 900, 950, 1 000, 1 050, 1 100, 1 150, 1 200, 1 250, and 1 300 h, respectively, and cuttings with vegetative buds were kept at 51°C until 1 500 h. The results indicated that pistachio cultivars requite chilling time between 750-1 400 h and heat requirements between 8 852-15 420 growing degree hours （GDH）. Consequently, Kalle-Ghuchi had the lowest chilling （750-950 h）, and heat （8 852-9 768 GDH） requirements, Ahmad-Aghaei and Owhadi had intermediate （1 000-1 250 h, 10 656- 13 320 GDH） and Akbari had the highest chilling （1 200-1 400 h） and heat （11 863-15 420 GDH） requirement.
基金supported by the National Natural Science Foundation of China(Grant No.32001996)Central Publicinterest Scientific Institution Basal Research Fund(Grant No.Y2022QC23)+2 种基金Agricultural Science and Technology Innovation Program(Grant No.CAAS-ASTIP-2021-ZFRI-01)the Crop Germplasm Resources Conservation Project(Grant No.2016NWB041)the Science and Technology Major Project of Yunnan(Gene mining and breeding of peach at highaltitude and low-latitude regions)。
文摘Bud dormancy facilitates the survival of meristems under harsh environmental conditions.To elucidate how molecular responses to chilling accumulation controlling dormancy in peach buds,chromatin immunoprecipitation sequencing to identify the H3K27me3 modifications and RNA sequencing of two peach cultivars with pronounced differences in chilling requirement were carried out,the results showed that genes associated with abscisic acid and gibberellic acid signal pathways play key roles in dormancy regulation.The results demonstrated that peach flower bud differentiation occurred continuously in both cultivars during chilling accumulation,which was correlated with the transcript abundance of key genes involved in phytohormone metabolism and flower bud development under adverse conditions.The more increased strength in high chillingrequirement cultivar along with the chilling accumulation at the genome-wide level.The function of the dormancy-associated MADS-box gene PpDAM6 was identified,which is involved in leaf bud break in peach and flower development in transgenic Nicotiana tabacum(NC89).In addition,PpDAM6 was positively regulated by PpCBF,and the genes of putative dormancy-related and associated with metabolic pathways were proposed.Taken together,these results constituted a theoretical basis for elucidating the regulation of peach bud dormancy transition.
