China’s domestic animation industry is deeply rooted in its rich traditional cultural heritage.While continuously exploring and showcasing the unique charm of such cultural heritage through storytelling,imagery estab...China’s domestic animation industry is deeply rooted in its rich traditional cultural heritage.While continuously exploring and showcasing the unique charm of such cultural heritage through storytelling,imagery establishment,and spirit creation,Chinese animation also seamlessly integrates modern aesthetic characteristics and cultural values into its development within the context of the new era.By adopting a contemporary perspective,it innovatively expresses the essence of traditional culture while striving to shape and present a reliable,admirable and respectable image of China.Chinese animation aims to create credible contemporary animation images by drawing inspiration from traditional Chinese cultural archetypes.It also seeks to revitalize admirable traditional cultural imagery using vibrant and prevailing ACGN visuals,while shaping credible characters with richer cultural connotations and crafting stories with more enchanting plots to convey the spiritual essence of the Chinese nation.By establishing reliable,admirable and respectable animation images,the Chinese animation industry strives to enhance its capability to“tell China’s stories well and make the voice of China heard,”to better promote the image of China in the new era from a global perspective.展开更多
By contrast to a slowdown in the world economy, China kept up a rapid growth rate, a relatively high quality of growth and low inflation for the year 2001. This is mainly due to the result of proactive fiscal policies...By contrast to a slowdown in the world economy, China kept up a rapid growth rate, a relatively high quality of growth and low inflation for the year 2001. This is mainly due to the result of proactive fiscal policies that the Chinese Government adopted to stimulate domestic demand. Domestic demand can be divided into investment demand and consumption demand,展开更多
China＇s first wave of economic globalization is characterized by its full utilization of foreign advanced, especially innovative, production factors in the domestic market to develop its innovation economy. China＇s ...China＇s first wave of economic globalization is characterized by its full utilization of foreign advanced, especially innovative, production factors in the domestic market to develop its innovation economy. China＇s participation in global economy can be described as fully utilizing domestic low-end production factors through foreign markets. This type of participation has been overdone. As China aims to engage in a second wave of economic globalization, the focus is to implement an in-depth globalization strategy under the condition of expanding domestic demands, namely and to develop the global economy based on domestic demands, The purpose is to achieve an economic rebalance with the world and help China out of its overcapacity predicament in weaker global economic climates. Moreover, it will bring China a chance to introduce foreign advanced factors to accelerate growth and develop innovation economy by turning economic crisis in the West into opportunities.展开更多
In 2009, China’s growth of national textile and apparel sales reached 20 percent, so that by 2010, domestic consumption has already become the bright spot in
Entering into the 1990s, Chinese scientists have made mice, drosophila and silkworm eggs experiments in space with satellites and achieved remarkable results. These animals were put in space environment units with the...Entering into the 1990s, Chinese scientists have made mice, drosophila and silkworm eggs experiments in space with satellites and achieved remarkable results. These animals were put in space environment units with the ability of adjusting pressure, temperature and moisture control, air conditioning and purifying as well as foods and water supply. After 8-days’ flight, all performance parameters were normal and met the design requirements. The two mice kept alive for 5 days and 10 hours展开更多
AIM: To study the purifying method and characteristics of new gosling viral enteritis virus (NGVEV),the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the patho...AIM: To study the purifying method and characteristics of new gosling viral enteritis virus (NGVEV),the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the pathomorphological development in goslings infected artificially with NGVEV. METHODS: (1)NGVEV virions were purified by the procedure of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfate radical and ammonium ion and separation by gel filtration chromatography, and SDS-PAGE. (2)Forty-2-day-old White Sichuan goslings were orally administered with NGVEV and 24 hr later 2 birds were randomly selected and killed at 24hr intervals until death occurred. Specimens(duodenum, ileum, liver, heart, kidney, spleen, lung, proventriculus, pancreas, esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and eosin and studied by light microscope. RESULTS: NGVEV shared the typical characteristics of Adenovirus and which structural proteins consisted of 15 polypeptides. Necrosis and sloughing of the epithelial cells covering the villus tips of the duodenum were first observed in goslings 2 days postinfection artificially with NGVEV. With the progress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of mucosa necrosis and inflammatory exudation of the small intestine, fibrinonecrotic enteritis was further developed and embolus composed of either intestinal contents wrapped by pseudo-membrane or of the mixture of fibrous exudate and necrotic intestinal mucosa were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen. Hemorrhage and hyperemia could be observed on the lung and kidney. Epithelial cells of the renal tubular underwent degeneration. In some cases, granular degeneration and fatty degeneration could be found in the liver and in some cases at a later stage of this disease the epithelial cells of trachea and proventriculus might be found sloughed. In some cases at an early stage of this disease, cardiac hyperemia and hemorrhage could be observed. Esophagus, pancreas and brain were found normal. Analyses and comparisons between the pathologic lesions of NGVE and Gosling Plague (GP) were available in this paper as well. CONCLUSION: (1)NGVEV is adenovirus. (2)Pathological characteristic could be as the data for NGVE diagnosis.展开更多
AIM: Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9(CYP2C9) is a polymorphic enzyme respon...AIM: Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9(CYP2C9) is a polymorphic enzyme responsible for the metabolism of a large number of clinically important drugs. It ranks among the most important drug metabolizing enzymes in humans. In order to provide a sufficient amount of the enzyme for drug metabolic research, the CYP2C9 cDNA was cloned and expressed stably in CHL cells. METHODS: After extraction of total RNA from human liver tissue, the human CYP2C9 cDNA was amplified with reverse transcription-polymerase chain reaction (RT-PCR), and cloned into cloning vector pGEM-T. The cDNA fragment was identified by DNA sequencing and subcloned into a mammalian expression vector pREP9. A transgenic cell line was established by transfecting the recombinant vector of pREP9-CYP2C9 into CHL cells. The enzyme activity of CYP2C9 catalyzing oxidation of tolbutamide to hydroxy tolbutamide in S9 fraction of the cell was determined by high performance liquid chromatography(HPLC). RESULTS: The amino acid sequence predicted from the cDNA segment was identical to that of CYP2C9*1, the wild type CYP2C9. However, there were two base differences, i.e. 21T】C, 1146C】T, but the encoding amino acid sequence was the same, L7, P382. The S9 fraction of the established cell line metabolizes tolbutamide to hydroxy tolbutamide; tolbutamide hydroxylase activity was found to be 0.465 +/- 0.109 micromol.min(-1).g(-1) S9 protein or 8.62 +/- 2.02mol.min(-1).mol(-1) CYP, but was undetectable in parental CHL cell. CONCLUSION: The cDNA of human CYP2C9 was successfully cloned and a cell line of CHL- CYP2C9, efficiently expressing the protein of CYP2C9, was established.展开更多
AIM: To explore the relevance of Maotai liquor and liver diseases. METHODS: Epidemiological study was conducted on groups of subjects, each consisting of 3 subjects from the Maotai liquor group consisting of 99 indivi...AIM: To explore the relevance of Maotai liquor and liver diseases. METHODS: Epidemiological study was conducted on groups of subjects, each consisting of 3 subjects from the Maotai liquor group consisting of 99 individuals and one from the non-alcoholic control group consisting of 33 individuals. Liver biopsy was performed on 23 volunteers from Guizhou Maotai Distillery who had a constant and long history of drinking Maotai liquor. Experimental histopathological study was conducted as follows: sixty male Wistar rats were divided into 3 groups randomly and fed with Maotai liquor, ordinary white wine, and physiological saline respectively for a period of 8 and 12 weeks. The rats were sacrificed in batches, then serum ALT, AST, TBil, and AKP were measured. Rat livers were harvested to measure the liver indexes, GSH, and MDA. Histopathological examinations were also performed. Another eighty mice were randomly divided into 4 groups and fed with Maotai (at different dosages of 10 ml.kg(-1) and 20 ml.kg(-1)), ethanol, and physiological saline. The animals were sacrificed after 4 weeks and serum ALT was determined. Then the livers were harvested and liver indexes and MDA were measured. RESULTS: The incidence rate of hepatic symptoms, splenomegaly, liver function impairment, reversal of Albumin/Globulin and increased diameter of portal veins in the Maotai liquor group were 1.0% 1/99 , 1.0% 1/99 , 1.0% 1/99 , 1.0% 1/99 , 0 0/99 and 0 0/99 , 0 0/99 ,0 0/99 , 0 0/99 , 0 0/99 , respectively. There was no significant difference between the Maotai group and the non-alcoholic control group P】0.05 . Various degree of fatty infiltration of hepatocytes was found in the 23 volunteers receiving liver biopsy, but there was no obvious hepatic fibrosis or cirrhosis. A comparison was made between the Maotai liquor group and the ordinary white wine group. It was found that hepatic MDA in rats and mice were 0.33+/-0.10 and 0.49+/-0.23 respectively in Maotai group and 0.61+/-0.22 and 0.66+/-0.32 in the ordinary white wine group; MDA had an obvious decrease in the Maotai liquor group (P【0.05); hepatic GSH were 0.12 mg.g(-1)+/-0.06 mg.g(-1) in rats of the Maotai liquor group and (0.08+/-0.02)mg.g(-1) in white wine group, it was obviously increased in the Maotai liquor group (P【0.05). After the 20 rats had been fed with ordinary white wine for 8 weeks consecutively, disarranged hepatocyte cords, fatty infiltration of hepatocytes, and fibrous septa of varying widths due to hepatic connective tissues proliferation were observed; after 12 weeks, the fibrous tissue proliferation continued and early cirrhosis appeared. Compared with the ordinary white wine group, fatty infiltration was observed in the 8-week and 12-week groups, but no necrosis or fibrosis or cirrhosis was found in the Maotai liquor group (P【0.05). CONCLUSION: Maotai liquor may cause fatty liver but not hepatic fibrosis or cirrhosis, and it can strengthen lipid peroxidation in the liver.展开更多
In the lOth Five-Year-Plan period, the textile industry will further underline the structural rationalization, and intensify the reform and renovation to set us a modern enterprise system, and encourage and direct the...In the lOth Five-Year-Plan period, the textile industry will further underline the structural rationalization, and intensify the reform and renovation to set us a modern enterprise system, and encourage and direct the growth of the non-stateowned economy. And new & high-tech, will be applied to the traditional industry and the enough attention will be given to sourcing the new potentials for economic growth, and also to actively exploring the international and domestic market展开更多
You can’t open a newspaper or turn on a TV in China without hearing about the rise of China,but,it’s easy to forget that China is a changed nation where successes aren’t simply dictated by the central authorities a...You can’t open a newspaper or turn on a TV in China without hearing about the rise of China,but,it’s easy to forget that China is a changed nation where successes aren’t simply dictated by the central authorities anymore.Today,there are any number of Chinese citizens changing the world in constructive and fascinating ways—scientists,entrepreneurs,journalists,activists,and so many more.This issue,we discuss six very special展开更多
Investment surged up continuously From Jan. to Nov. 2009, total statistics-worthy fixed-assets investment (over CNY 5 million) in the textile industry was up 9.53%
基金funded by “Multi-dimensional Reconstruction and Symbol Hybridity:Research on Innovative Expression of Traditional Culture in Domestic Animated Films”(WHCY2023B28)a project funded by the Cultural Industry Development Research Center of Sichuan Provincial Key Research Base of Philosophy and Social Sciences+1 种基金funded by “An Intertextual Analysis of Li Bing’s Story from a Cross-media Perspective”(RX2300002875)a research project funded by the Li Bing Research Center at the Sichuan Provincial Key Research Base of Philosophy and Social Sciences
文摘China’s domestic animation industry is deeply rooted in its rich traditional cultural heritage.While continuously exploring and showcasing the unique charm of such cultural heritage through storytelling,imagery establishment,and spirit creation,Chinese animation also seamlessly integrates modern aesthetic characteristics and cultural values into its development within the context of the new era.By adopting a contemporary perspective,it innovatively expresses the essence of traditional culture while striving to shape and present a reliable,admirable and respectable image of China.Chinese animation aims to create credible contemporary animation images by drawing inspiration from traditional Chinese cultural archetypes.It also seeks to revitalize admirable traditional cultural imagery using vibrant and prevailing ACGN visuals,while shaping credible characters with richer cultural connotations and crafting stories with more enchanting plots to convey the spiritual essence of the Chinese nation.By establishing reliable,admirable and respectable animation images,the Chinese animation industry strives to enhance its capability to“tell China’s stories well and make the voice of China heard,”to better promote the image of China in the new era from a global perspective.
文摘By contrast to a slowdown in the world economy, China kept up a rapid growth rate, a relatively high quality of growth and low inflation for the year 2001. This is mainly due to the result of proactive fiscal policies that the Chinese Government adopted to stimulate domestic demand. Domestic demand can be divided into investment demand and consumption demand,
文摘China＇s first wave of economic globalization is characterized by its full utilization of foreign advanced, especially innovative, production factors in the domestic market to develop its innovation economy. China＇s participation in global economy can be described as fully utilizing domestic low-end production factors through foreign markets. This type of participation has been overdone. As China aims to engage in a second wave of economic globalization, the focus is to implement an in-depth globalization strategy under the condition of expanding domestic demands, namely and to develop the global economy based on domestic demands, The purpose is to achieve an economic rebalance with the world and help China out of its overcapacity predicament in weaker global economic climates. Moreover, it will bring China a chance to introduce foreign advanced factors to accelerate growth and develop innovation economy by turning economic crisis in the West into opportunities.
文摘In 2009, China’s growth of national textile and apparel sales reached 20 percent, so that by 2010, domestic consumption has already become the bright spot in
文摘Entering into the 1990s, Chinese scientists have made mice, drosophila and silkworm eggs experiments in space with satellites and achieved remarkable results. These animals were put in space environment units with the ability of adjusting pressure, temperature and moisture control, air conditioning and purifying as well as foods and water supply. After 8-days’ flight, all performance parameters were normal and met the design requirements. The two mice kept alive for 5 days and 10 hours
基金Supported by the Mational Mature Scientific Foundation of China,No.39970561Funds for the Leeders in Academia of Sichuan Province,No.9900009240018.
文摘AIM: To study the purifying method and characteristics of new gosling viral enteritis virus (NGVEV),the etiological agent of new gosling viral enteritis (NGVE) which was first recognized in China, as well as the pathomorphological development in goslings infected artificially with NGVEV. METHODS: (1)NGVEV virions were purified by the procedure of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfate radical and ammonium ion and separation by gel filtration chromatography, and SDS-PAGE. (2)Forty-2-day-old White Sichuan goslings were orally administered with NGVEV and 24 hr later 2 birds were randomly selected and killed at 24hr intervals until death occurred. Specimens(duodenum, ileum, liver, heart, kidney, spleen, lung, proventriculus, pancreas, esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and eosin and studied by light microscope. RESULTS: NGVEV shared the typical characteristics of Adenovirus and which structural proteins consisted of 15 polypeptides. Necrosis and sloughing of the epithelial cells covering the villus tips of the duodenum were first observed in goslings 2 days postinfection artificially with NGVEV. With the progress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jejunum tended to be involved. With the intensification of mucosa necrosis and inflammatory exudation of the small intestine, fibrinonecrotic enteritis was further developed and embolus composed of either intestinal contents wrapped by pseudo-membrane or of the mixture of fibrous exudate and necrotic intestinal mucosa were observed in the middle-lower part of the small intestine. This structure occluded the intestinal tract and made the intestine dilated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen. Hemorrhage and hyperemia could be observed on the lung and kidney. Epithelial cells of the renal tubular underwent degeneration. In some cases, granular degeneration and fatty degeneration could be found in the liver and in some cases at a later stage of this disease the epithelial cells of trachea and proventriculus might be found sloughed. In some cases at an early stage of this disease, cardiac hyperemia and hemorrhage could be observed. Esophagus, pancreas and brain were found normal. Analyses and comparisons between the pathologic lesions of NGVE and Gosling Plague (GP) were available in this paper as well. CONCLUSION: (1)NGVEV is adenovirus. (2)Pathological characteristic could be as the data for NGVE diagnosis.
基金National Natural Science Foundation of China,No.39770868Natural Science Foundation of Zhejiang Province,No.397490
文摘AIM: Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9(CYP2C9) is a polymorphic enzyme responsible for the metabolism of a large number of clinically important drugs. It ranks among the most important drug metabolizing enzymes in humans. In order to provide a sufficient amount of the enzyme for drug metabolic research, the CYP2C9 cDNA was cloned and expressed stably in CHL cells. METHODS: After extraction of total RNA from human liver tissue, the human CYP2C9 cDNA was amplified with reverse transcription-polymerase chain reaction (RT-PCR), and cloned into cloning vector pGEM-T. The cDNA fragment was identified by DNA sequencing and subcloned into a mammalian expression vector pREP9. A transgenic cell line was established by transfecting the recombinant vector of pREP9-CYP2C9 into CHL cells. The enzyme activity of CYP2C9 catalyzing oxidation of tolbutamide to hydroxy tolbutamide in S9 fraction of the cell was determined by high performance liquid chromatography(HPLC). RESULTS: The amino acid sequence predicted from the cDNA segment was identical to that of CYP2C9*1, the wild type CYP2C9. However, there were two base differences, i.e. 21T】C, 1146C】T, but the encoding amino acid sequence was the same, L7, P382. The S9 fraction of the established cell line metabolizes tolbutamide to hydroxy tolbutamide; tolbutamide hydroxylase activity was found to be 0.465 +/- 0.109 micromol.min(-1).g(-1) S9 protein or 8.62 +/- 2.02mol.min(-1).mol(-1) CYP, but was undetectable in parental CHL cell. CONCLUSION: The cDNA of human CYP2C9 was successfully cloned and a cell line of CHL- CYP2C9, efficiently expressing the protein of CYP2C9, was established.
基金The primary sciences and technology project of Guizhou province,No.19992015
文摘AIM: To explore the relevance of Maotai liquor and liver diseases. METHODS: Epidemiological study was conducted on groups of subjects, each consisting of 3 subjects from the Maotai liquor group consisting of 99 individuals and one from the non-alcoholic control group consisting of 33 individuals. Liver biopsy was performed on 23 volunteers from Guizhou Maotai Distillery who had a constant and long history of drinking Maotai liquor. Experimental histopathological study was conducted as follows: sixty male Wistar rats were divided into 3 groups randomly and fed with Maotai liquor, ordinary white wine, and physiological saline respectively for a period of 8 and 12 weeks. The rats were sacrificed in batches, then serum ALT, AST, TBil, and AKP were measured. Rat livers were harvested to measure the liver indexes, GSH, and MDA. Histopathological examinations were also performed. Another eighty mice were randomly divided into 4 groups and fed with Maotai (at different dosages of 10 ml.kg(-1) and 20 ml.kg(-1)), ethanol, and physiological saline. The animals were sacrificed after 4 weeks and serum ALT was determined. Then the livers were harvested and liver indexes and MDA were measured. RESULTS: The incidence rate of hepatic symptoms, splenomegaly, liver function impairment, reversal of Albumin/Globulin and increased diameter of portal veins in the Maotai liquor group were 1.0% 1/99 , 1.0% 1/99 , 1.0% 1/99 , 1.0% 1/99 , 0 0/99 and 0 0/99 , 0 0/99 ,0 0/99 , 0 0/99 , 0 0/99 , respectively. There was no significant difference between the Maotai group and the non-alcoholic control group P】0.05 . Various degree of fatty infiltration of hepatocytes was found in the 23 volunteers receiving liver biopsy, but there was no obvious hepatic fibrosis or cirrhosis. A comparison was made between the Maotai liquor group and the ordinary white wine group. It was found that hepatic MDA in rats and mice were 0.33+/-0.10 and 0.49+/-0.23 respectively in Maotai group and 0.61+/-0.22 and 0.66+/-0.32 in the ordinary white wine group; MDA had an obvious decrease in the Maotai liquor group (P【0.05); hepatic GSH were 0.12 mg.g(-1)+/-0.06 mg.g(-1) in rats of the Maotai liquor group and (0.08+/-0.02)mg.g(-1) in white wine group, it was obviously increased in the Maotai liquor group (P【0.05). After the 20 rats had been fed with ordinary white wine for 8 weeks consecutively, disarranged hepatocyte cords, fatty infiltration of hepatocytes, and fibrous septa of varying widths due to hepatic connective tissues proliferation were observed; after 12 weeks, the fibrous tissue proliferation continued and early cirrhosis appeared. Compared with the ordinary white wine group, fatty infiltration was observed in the 8-week and 12-week groups, but no necrosis or fibrosis or cirrhosis was found in the Maotai liquor group (P【0.05). CONCLUSION: Maotai liquor may cause fatty liver but not hepatic fibrosis or cirrhosis, and it can strengthen lipid peroxidation in the liver.
文摘In the lOth Five-Year-Plan period, the textile industry will further underline the structural rationalization, and intensify the reform and renovation to set us a modern enterprise system, and encourage and direct the growth of the non-stateowned economy. And new & high-tech, will be applied to the traditional industry and the enough attention will be given to sourcing the new potentials for economic growth, and also to actively exploring the international and domestic market
文摘You can’t open a newspaper or turn on a TV in China without hearing about the rise of China,but,it’s easy to forget that China is a changed nation where successes aren’t simply dictated by the central authorities anymore.Today,there are any number of Chinese citizens changing the world in constructive and fascinating ways—scientists,entrepreneurs,journalists,activists,and so many more.This issue,we discuss six very special
文摘Investment surged up continuously From Jan. to Nov. 2009, total statistics-worthy fixed-assets investment (over CNY 5 million) in the textile industry was up 9.53%
