BACKGROUND Allergic cutaneous vasculitis(ACV)is a difficult disease to treat.At present,there is no effective treatment for this condition.Traditionally,immunosuppressants and hormones have been primarily used in its ...BACKGROUND Allergic cutaneous vasculitis(ACV)is a difficult disease to treat.At present,there is no effective treatment for this condition.Traditionally,immunosuppressants and hormones have been primarily used in its management,but the treatment effect is suboptimal,and it has several side effects.CASE SUMMARY We present the case of a 19-year-old woman who presented at our hospital with a four-year history of symmetric skin lesions mainly affecting her lower extremities.She had previously undergone treatment with prednisolone acetate,cetirizine hydrochloride,and loratadine tablets but had not experienced any relief in her condition.Thereafter,she was treated with oral traditional Chinese medicine.Her skin damage gradually improved within two months of treatment initiation.After six months,the skin ulcers had completely subsided.No evidence of skin ulcer recurrence was observed during the subsequent follow-up.This report presents the first case of a female patient who received oral Danggui Sini decoction for the treatment of ACV.CONCLUSION Danggui Sini decoction may be a promising oral treatment for ACV patients.展开更多
Acne vulgaris is a chronic inflammatory skin disease damaging face,and the incidence of acne vulgaris is increasing year by year in China.Acne falls into the category of pimple in traditional Chinese medicine,and ther...Acne vulgaris is a chronic inflammatory skin disease damaging face,and the incidence of acne vulgaris is increasing year by year in China.Acne falls into the category of pimple in traditional Chinese medicine,and there are many dialectical understandings of acne in modern traditional Chinese medicine.Dr.He Aijuan has a unique diagnosis and treatment thinking in treating acne vulgaris(liver stagnation and spleen deficiency type)with traditional Chinese medicine in her clinical work for many years.Her application of Danggui Shaoyao Powder combined with Sini Powder has a remarkable effect in the clinical treatment process,so a clinical medical case is selected for discussion.展开更多
Background:Chinese medicine has been proposed as a novel approach to the prevention of metabolic disorders such as obesity.Danggui Buxue decoction,a decoction prepared from Huangqi(Astragali Radix)and Danggui(Angelica...Background:Chinese medicine has been proposed as a novel approach to the prevention of metabolic disorders such as obesity.Danggui Buxue decoction,a decoction prepared from Huangqi(Astragali Radix)and Danggui(Angelicae Sinensis Radix),has been used to nourish vitality and enhance blood circulation in traditional Chinese medicine.However,the effect of Danggui Buxue decoction on obesity is still primarily unknown.Methods:Cell proliferation,differentiation,and apolipoprotein-IV transcription were investigated to explore the function of Danggui Buxue decoction by methyl thiazolyl tetrazolium assay,alkaline phosphatase assay,and luciferase assay,respectively.Results:Danggui Buxue decoction promoted cell growth by up to 15%(P=0.034)and induced cell differentiation by up to 38%(P=0.006)at 0.3 mg/mL.Moreover,Danggui Buxue decoction enhanced the transcription of apolipoprotein-IV by 2.4 times(P=0.027),activating its promoter by 28.9%(P=0.031)at 0.3 mg/mL.In addition,Danggui Buxue decoction functioned in a dose-dependent manner.Conclusion:These results suggest that Danggui Buxue decoction promotes cell differentiation by enhancing apolipoprotein-IV transcription and alkaline phosphatase activity,and it may also have a potential anti-obesity effect because apolipoprotein-IV transcription is closely related to the reduction of food intake.展开更多
Backgroud:Danggui Buxue decoction(DBD),a classical prescription in traditional Chinese medicine,has been found to have protective effect on bleomycin-induced pulmonary fibrosis in rats by reducing alveolar inflammatio...Backgroud:Danggui Buxue decoction(DBD),a classical prescription in traditional Chinese medicine,has been found to have protective effect on bleomycin-induced pulmonary fibrosis in rats by reducing alveolar inflammation and fibrosis.However,the biological activity of individual chemical components and mechanism of action of whole formula are not clear.Methods:Potential targets of active ingredients of DBD were collected through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and SymMap database.Target genes related to idiopathic pulmonary fibrosis were obtained from the Online Mendelian Inheritance in Man database,Therapeutic Targets Database and Gkb database.Then,the common targets were obtained by overlapping the potential targets of active ingredients in DBD and diseases related targets.The selected targets were subjected to Kyoto Encyclopedia of Genes and Genomes signaling pathway and Gene Ontology analysis,and the network map of active component-target-pathway was established using Cytoscape 3.7.1 software.The active components of DBD with most targets were selected for fibrosis-related marker verification.The mRNA and protein expression of fibrosis markers,α-smooth muscle actin,collagen 1 and fibronectin,were detected in TGF-β1-induced fibroblast cell line after treatment with the active components.Results:The 14 active ingredients,such as quercetin and kaempferol,were screened from DBD.It acts on 26 targets like estrogen receptor 2 and prostaglandin-endoperoxide synthase 2,and mainly involves 38 signaling pathways such as cell inflammation and autophagy.Kaempferol and quercetin are the two compounds with the highest network regulation,which can inhibit the transformation of fibroblasts into myofibroblasts and reduce the expression of fibrosis markersα-smooth muscle actin,collagen 1 and fibronectin.Conclusion:The integration mode of multi-component,multi-target,multi-channel and mechanism of DBD in the treatment of idiopathic pulmonary fibrosis are predicted by means of network pharmacology.Our study could indicate the direction of further anti-fibrotic mechanism research.展开更多
Idiopathic pulmonary fibrosis(IPF)is a chronic,progressive,fibrotic interstitial lung disease.Current treatment options for IPF are limited.Radix Astragali(RA)and Radix Angelicae Sinensis(RAS),according to 5:1 ratio c...Idiopathic pulmonary fibrosis(IPF)is a chronic,progressive,fibrotic interstitial lung disease.Current treatment options for IPF are limited.Radix Astragali(RA)and Radix Angelicae Sinensis(RAS),according to 5:1 ratio composed of Danggui Buxue decoction(DGBXD),which have played an essential role in the treatment of IPF.This article reviewed the experimental research,clinical research,and progress of RA and RAS(DGBXD)treating IPF to provide a deeper scientific basis for the future experimental research and clinical research.展开更多
目的研究当归芍药散(Danggui Shaoyao San,DSS)对糖尿病肾病小鼠肠道菌群的作用。方法选取30只8周龄雄性db/db小鼠,6只8周龄雄性db/m小鼠。检测空腹血糖以及尿白蛋白/肌酐比值(urinary albumin to creatinine ratio,UACR),将符合要求的d...目的研究当归芍药散(Danggui Shaoyao San,DSS)对糖尿病肾病小鼠肠道菌群的作用。方法选取30只8周龄雄性db/db小鼠,6只8周龄雄性db/m小鼠。检测空腹血糖以及尿白蛋白/肌酐比值(urinary albumin to creatinine ratio,UACR),将符合要求的db/db小鼠随机分成5组:模型(MOD)组(0.1 mL/10 g蒸馏水)、达格列净(DAPA)组(1.3 mg/kg达格列净片)及DSS低剂量(DSS-L)组(8.39 g/kg)、中剂量(DSS-M)组(16.77 g/kg)、高剂量(DSS-H)组(33.54 g/kg),每组6只;6只db/m小鼠为空白对照(CON)组(0.1 m L/10 g蒸馏水)。测定小鼠空腹血糖、胰岛素耐量;ELISA法测定血尿素氮和血肌酐;全自动生化分析仪检测UACR;对小鼠的肾脏组织和小肠组织进行HE染色;取小鼠结肠粪便进行16S r RNA测序。结果与CON组比较,MOD组糖耐量下降,空腹血糖、血尿素氮、血肌酐、UACR升高(P<0.01);与MOD组比较,DSS组的糖耐量升高(P<0.01),空腹血糖、血尿素氮、血肌酐、UACR降低(P<0.01),肾脏和小肠的病理损伤程度降低。肠道菌群多样性研究结果显示:相比CON组,MOD组肠道菌群多样性降低;相比MOD组,DSS-M组与CON组的菌群差异性较小。在菌群的物种组成方面,DSS-M组小鼠嗜黏蛋白阿克曼氏菌(Akkermansia muciniphila)的相对丰度明显增加(P<0.01)。结论DSS能显著增加糖尿病肾病db/db小鼠肠道中的嗜黏蛋白阿克曼氏菌的丰度,可能是其改善糖尿病肾病的机制之一。展开更多
OBJECTIVE: To investigate the effect of the traditional Chinese herbs Astragali and Angelicae Sinensis(A & As) particle [contains Huangqi(Radix Astragali Mongolica), Danggui(Radix Angelicae Sinensis),Huzhanggeng(R...OBJECTIVE: To investigate the effect of the traditional Chinese herbs Astragali and Angelicae Sinensis(A & As) particle [contains Huangqi(Radix Astragali Mongolica), Danggui(Radix Angelicae Sinensis),Huzhanggeng(Rhizoma Polygoni Cuspidati) and Danshen(Radix Salviae Miltiorrhizae)] on proteinuria in glomerulonephritis patients with stage 2 chronic kidney disease.METHODS: A prospective, multi-center, and randomized controlled clinical trial was performed for 24 weeks. From March 2011 to April 2012, 158 patients from nine hospitals in China participated.They were randomized into the A&As group(79 cases, A&As particle 15.2 g/day) and losartan group(79 cases, losartan 50 mg/day). At each follow-up visit, clinical data including blood pressure, urinalysis, 24-h-urinary protein excretion, serum albumin and serum creatinine were collected.RESULTS: All 158 patients completed the follow-up. Proteinuria in the losartan group exhibited a biphasic time-dependent decline with a significant steady reduction from baseline to week 12(P = 0.0014), and a platform level during the remaining 12-week follow-up(P > 0.05). In contrast, there was a continual significant decrease of proteinuria in the A & As group(P < 0.001). When compared with the losartan results, proteinuria in the A & As group from week 16 to week 24 was significantly reduced(P < 0.001). Stable e GFRs and blood pressure were also observed in both groups. Medication side effects were minimal and non-fatal.CONCLUSION: For Chinese glomerulonephritis patients with stage 2 chronic kidney disease, therapy with A & As particles may provide effective anti-proteinuria treatment.展开更多
文摘BACKGROUND Allergic cutaneous vasculitis(ACV)is a difficult disease to treat.At present,there is no effective treatment for this condition.Traditionally,immunosuppressants and hormones have been primarily used in its management,but the treatment effect is suboptimal,and it has several side effects.CASE SUMMARY We present the case of a 19-year-old woman who presented at our hospital with a four-year history of symmetric skin lesions mainly affecting her lower extremities.She had previously undergone treatment with prednisolone acetate,cetirizine hydrochloride,and loratadine tablets but had not experienced any relief in her condition.Thereafter,she was treated with oral traditional Chinese medicine.Her skin damage gradually improved within two months of treatment initiation.After six months,the skin ulcers had completely subsided.No evidence of skin ulcer recurrence was observed during the subsequent follow-up.This report presents the first case of a female patient who received oral Danggui Sini decoction for the treatment of ACV.CONCLUSION Danggui Sini decoction may be a promising oral treatment for ACV patients.
文摘Acne vulgaris is a chronic inflammatory skin disease damaging face,and the incidence of acne vulgaris is increasing year by year in China.Acne falls into the category of pimple in traditional Chinese medicine,and there are many dialectical understandings of acne in modern traditional Chinese medicine.Dr.He Aijuan has a unique diagnosis and treatment thinking in treating acne vulgaris(liver stagnation and spleen deficiency type)with traditional Chinese medicine in her clinical work for many years.Her application of Danggui Shaoyao Powder combined with Sini Powder has a remarkable effect in the clinical treatment process,so a clinical medical case is selected for discussion.
基金supported by Natural Science Foundation of Guangdong Province of China(2018A030307074)Project of Educational Commission of Guangdong Province of China(2019KQNCX080)。
文摘Background:Chinese medicine has been proposed as a novel approach to the prevention of metabolic disorders such as obesity.Danggui Buxue decoction,a decoction prepared from Huangqi(Astragali Radix)and Danggui(Angelicae Sinensis Radix),has been used to nourish vitality and enhance blood circulation in traditional Chinese medicine.However,the effect of Danggui Buxue decoction on obesity is still primarily unknown.Methods:Cell proliferation,differentiation,and apolipoprotein-IV transcription were investigated to explore the function of Danggui Buxue decoction by methyl thiazolyl tetrazolium assay,alkaline phosphatase assay,and luciferase assay,respectively.Results:Danggui Buxue decoction promoted cell growth by up to 15%(P=0.034)and induced cell differentiation by up to 38%(P=0.006)at 0.3 mg/mL.Moreover,Danggui Buxue decoction enhanced the transcription of apolipoprotein-IV by 2.4 times(P=0.027),activating its promoter by 28.9%(P=0.031)at 0.3 mg/mL.In addition,Danggui Buxue decoction functioned in a dose-dependent manner.Conclusion:These results suggest that Danggui Buxue decoction promotes cell differentiation by enhancing apolipoprotein-IV transcription and alkaline phosphatase activity,and it may also have a potential anti-obesity effect because apolipoprotein-IV transcription is closely related to the reduction of food intake.
文摘Backgroud:Danggui Buxue decoction(DBD),a classical prescription in traditional Chinese medicine,has been found to have protective effect on bleomycin-induced pulmonary fibrosis in rats by reducing alveolar inflammation and fibrosis.However,the biological activity of individual chemical components and mechanism of action of whole formula are not clear.Methods:Potential targets of active ingredients of DBD were collected through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and SymMap database.Target genes related to idiopathic pulmonary fibrosis were obtained from the Online Mendelian Inheritance in Man database,Therapeutic Targets Database and Gkb database.Then,the common targets were obtained by overlapping the potential targets of active ingredients in DBD and diseases related targets.The selected targets were subjected to Kyoto Encyclopedia of Genes and Genomes signaling pathway and Gene Ontology analysis,and the network map of active component-target-pathway was established using Cytoscape 3.7.1 software.The active components of DBD with most targets were selected for fibrosis-related marker verification.The mRNA and protein expression of fibrosis markers,α-smooth muscle actin,collagen 1 and fibronectin,were detected in TGF-β1-induced fibroblast cell line after treatment with the active components.Results:The 14 active ingredients,such as quercetin and kaempferol,were screened from DBD.It acts on 26 targets like estrogen receptor 2 and prostaglandin-endoperoxide synthase 2,and mainly involves 38 signaling pathways such as cell inflammation and autophagy.Kaempferol and quercetin are the two compounds with the highest network regulation,which can inhibit the transformation of fibroblasts into myofibroblasts and reduce the expression of fibrosis markersα-smooth muscle actin,collagen 1 and fibronectin.Conclusion:The integration mode of multi-component,multi-target,multi-channel and mechanism of DBD in the treatment of idiopathic pulmonary fibrosis are predicted by means of network pharmacology.Our study could indicate the direction of further anti-fibrotic mechanism research.
基金Jiangyin Hospital of Traditional Chinese Medicine(202014 to YF Zhang)Grants from the Wuxi Health Commission’s Scientific Research Project(M202154 to YF Zhang)。
文摘Idiopathic pulmonary fibrosis(IPF)is a chronic,progressive,fibrotic interstitial lung disease.Current treatment options for IPF are limited.Radix Astragali(RA)and Radix Angelicae Sinensis(RAS),according to 5:1 ratio composed of Danggui Buxue decoction(DGBXD),which have played an essential role in the treatment of IPF.This article reviewed the experimental research,clinical research,and progress of RA and RAS(DGBXD)treating IPF to provide a deeper scientific basis for the future experimental research and clinical research.
文摘目的研究当归芍药散(Danggui Shaoyao San,DSS)对糖尿病肾病小鼠肠道菌群的作用。方法选取30只8周龄雄性db/db小鼠,6只8周龄雄性db/m小鼠。检测空腹血糖以及尿白蛋白/肌酐比值(urinary albumin to creatinine ratio,UACR),将符合要求的db/db小鼠随机分成5组:模型(MOD)组(0.1 mL/10 g蒸馏水)、达格列净(DAPA)组(1.3 mg/kg达格列净片)及DSS低剂量(DSS-L)组(8.39 g/kg)、中剂量(DSS-M)组(16.77 g/kg)、高剂量(DSS-H)组(33.54 g/kg),每组6只;6只db/m小鼠为空白对照(CON)组(0.1 m L/10 g蒸馏水)。测定小鼠空腹血糖、胰岛素耐量;ELISA法测定血尿素氮和血肌酐;全自动生化分析仪检测UACR;对小鼠的肾脏组织和小肠组织进行HE染色;取小鼠结肠粪便进行16S r RNA测序。结果与CON组比较,MOD组糖耐量下降,空腹血糖、血尿素氮、血肌酐、UACR升高(P<0.01);与MOD组比较,DSS组的糖耐量升高(P<0.01),空腹血糖、血尿素氮、血肌酐、UACR降低(P<0.01),肾脏和小肠的病理损伤程度降低。肠道菌群多样性研究结果显示:相比CON组,MOD组肠道菌群多样性降低;相比MOD组,DSS-M组与CON组的菌群差异性较小。在菌群的物种组成方面,DSS-M组小鼠嗜黏蛋白阿克曼氏菌(Akkermansia muciniphila)的相对丰度明显增加(P<0.01)。结论DSS能显著增加糖尿病肾病db/db小鼠肠道中的嗜黏蛋白阿克曼氏菌的丰度,可能是其改善糖尿病肾病的机制之一。
基金Ministry of Scientific Technology of China Grant(Optimum Proposal of Traditional Chinese Medicine on Proteinuria in Patients with Primary Glomerulonephritis,No.201007005)Shanghai Science&Technology Commission Grant(Clinical Study of Astragali Xiaobai Particle on Proteinuria in Patients with Primary Chronic Glomerulonephritis,No.14401972203)+2 种基金Three Years of Development Project for Traditional Chinese Medicine of Shanghai Municipal Commission of Health and Family Planning(Senior Chinese Integrative Medicine Talent Cultivation Project,No.ZYSNXD012-RC-ZXY003)Three Years of Development Project for Traditional Chinese Medicine of Shanghai Municipal Commission of Health and Family Planning(Effect of Astragali Xiaobai Particle on Proteinuria in Patients with Primary Chronic Glomerulonephritis,No.ZY3-JSFC-2-1029)Innovative Research Team in Universities,Shanghai Municipal Education
文摘OBJECTIVE: To investigate the effect of the traditional Chinese herbs Astragali and Angelicae Sinensis(A & As) particle [contains Huangqi(Radix Astragali Mongolica), Danggui(Radix Angelicae Sinensis),Huzhanggeng(Rhizoma Polygoni Cuspidati) and Danshen(Radix Salviae Miltiorrhizae)] on proteinuria in glomerulonephritis patients with stage 2 chronic kidney disease.METHODS: A prospective, multi-center, and randomized controlled clinical trial was performed for 24 weeks. From March 2011 to April 2012, 158 patients from nine hospitals in China participated.They were randomized into the A&As group(79 cases, A&As particle 15.2 g/day) and losartan group(79 cases, losartan 50 mg/day). At each follow-up visit, clinical data including blood pressure, urinalysis, 24-h-urinary protein excretion, serum albumin and serum creatinine were collected.RESULTS: All 158 patients completed the follow-up. Proteinuria in the losartan group exhibited a biphasic time-dependent decline with a significant steady reduction from baseline to week 12(P = 0.0014), and a platform level during the remaining 12-week follow-up(P > 0.05). In contrast, there was a continual significant decrease of proteinuria in the A & As group(P < 0.001). When compared with the losartan results, proteinuria in the A & As group from week 16 to week 24 was significantly reduced(P < 0.001). Stable e GFRs and blood pressure were also observed in both groups. Medication side effects were minimal and non-fatal.CONCLUSION: For Chinese glomerulonephritis patients with stage 2 chronic kidney disease, therapy with A & As particles may provide effective anti-proteinuria treatment.
