Effect of Yiqi Huoxue Recipe(益气活血方)on Cardiac Function and Ultrastructure in Regression of Pressure Overload-induced Myocardial Hypertrophy in Rats

Objective： To investigate the effect of Yiqi Huoxue Recipe （YHR, 益气活血方） on the cardiac function and ultrastructure during the regression of myocardial hypertrophy induced by pressure overload in rats. Methods： The model of myocardial hypertrophy was established by abdominal aortic banding. Eighty male Wistar rats were divided into six groups, the normal control group Ⅰ （n=20）, the normal control group Ⅱ（n=12）, the hypertension model group Ⅰ （n=12）, the hypertension model group Ⅱ （n=12）, the YHR group （n=12） and the Captopril group （n=12）. The observation was carried out in the normal control group Ⅰ and the hypertension model groupⅠ after 4 weeks of modeling, and the other four groups were observed after 16 weeks of modeling （12 weeks of administration）. The cardiac function was measured with a multichannel biological signal analysis system, and the myocardium ultrastructure was observed by a transmission electron microscope. Results： （1） Compared with the normal control group Ⅰ, the systolic blood pressure and cardiac coefficient （left ventricular weight/body weight） in the model Ⅰ group was higher （P〈0.05, P〈0.01）. （2） In the YHR group, cardiac coefficient and -dp/dtmax were lower, left ventricular systolic pressure and ＋dp/dtmin were higher when compared with the model group Ⅱ and the Captopril group （P〈0.05 or P〈0.01）. In the Captopril group, only cardiac coefficient was lower when compared with the mode group Ⅱ （P〈0.05）. （3） Compared with the normal control group Ⅱ, ＋dp/dtmax was higher （P〈0.01）, -dp/dtmax and isovolumetric contraction time （ICT） was lower （P〈0.05, P〈0.01） in both the YHR group and the Captopril group. （4） Results of the myocardium ultrastructure showed edema under myocardium plasmalemma, enlarged sarcoplasmic reticulum and T tube, and significantly enlarged intercalated disc of the cardiac muscle in the model groups. In the Captopril group, the extension of sarcoplasmic reticulum and T tube as well as the pathological changes of intercalated disc were lighter, with slight edema under the myocardium plasmalemma. In the YHR group, the expansion of the sarcoplasmic reticulum was less than in the Captopril group, part of the pathological changes of intercalated discs was slightly more severe than that in the Captopril group, the dissolution of nuclear chromatin was not found, which was similar to that of the Captopril group, and no injury of the nucleus was found, either. Conclusion： YHR could reverse myocardial hypertrophy in rats with abdominal aortic banding and improve the systolic and diastolic function of the left ventricle. The ultrastructure of the myocardium such as arcoplasmic reticulum, intercalated disc, and cell nucleus in abdominal aortic banding rats could be partly reversed by the recipe.

益气活血方逆转压力负荷性心肌肥大过程中心肌间血管超微结构的变化

目的观察益气活血方治疗压力负荷所致大鼠心肌肥大对心肌间血管超微结构的影响。方法用腹主动脉缩窄法制备心肌肥大模型,用多道生物信号分析系统记录血压和心率,用透射电镜观察心肌的超微结构。结果高血压组微血管内皮细胞部分高度肿胀,细胞增大是正常的6-7倍;微血管内皮细胞形成指状增生、指状分叉;微血管呈扩张状,内皮细胞间隙显著开大,内质网扩张。开搏通组未见内皮细胞水肿、肿胀,未见内皮细胞肿胀所致阻塞。中药组微血管内皮损害程度大部分均接近开搏通组,仅偶见内皮水肿程度略高于开搏通组。结论压力负荷性心肌肥大出现严重的微循环紊乱,益气活血方能大部分逆转腹主动脉缩窄大鼠的心肌间血管超微结构,内皮细胞水肿、肿胀减轻。

益气活血方逆转心肌肥大过程中心肌超微结构的变化

【目的】观察益气活血方治疗腹主动脉缩窄大鼠所致心肌肥大对心肌超微结构的影响。【方法】用多道生物信号系统记录血压和心率,用电子显微镜观察心肌超微结构的变化。【结果】(1)压力负荷所致心肌肥大表现肌膜下水肿,肌质网及T管扩张,心肌闰盘间隙显著扩大。(2)开搏通组的肌质网及T管扩张程度减轻,闰盘病变程度轻,大部分心肌肌膜下水肿不明显。中药组的肌质网扩张程度轻于开搏通组,部分闰盘损害略重于开搏通组。未见核染色质溶解,也未见高血压Ⅱ组的核损伤。【结论】益气活血方能部分逆转腹主动脉缩窄大鼠的心肌超微结构,如肌质网、闰盘、细胞核。

祛腐生肌膏敷料配合高压氧舱治疗感染性伤口80例临床观察

目的观察自制祛腐生肌膏敷料配合高压氧舱治疗感染性伤口的临床疗效。方法将156例感染性伤口患者随机分为2组。2组均予0.2%碘伏稀释液、0.9%氯化钠注射液及3%双氧水消毒冲洗伤口,祛除坏死组织,并根据实验室细菌培养加药敏试验结果,常规予敏感抗生素。治疗组80例予祛腐生肌膏敷料敷于创面或填塞入脓腔直至底部并配合高压氧舱治疗,对照组76例以0.5%碘伏纱块或纱条湿敷创面或填塞脓腔。2组均治疗9日后观察伤口面积的变化,2周后观察临床疗效及愈合时间。结果2组治疗9日后伤口面积比较差异有统计学意义(P<0.01);2组治疗2周后临床疗效及愈合时间比较差异有统计学意义(P<0.05),治疗组明显优于对照组。结论自制祛腐生肌膏敷料配合高压氧舱治疗感染性伤口疗效确切。