An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow( Gobiocypris rarus), which are critical to nutr...An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow( Gobiocypris rarus), which are critical to nutrition standardization for model fi sh. Twenty-four diets were formulated to contain three gross energy(10, 12.5, 15 kJ/g), four protein(20%, 25%, 30%, 35%), and two lipid levels(3%, 6%). The results showed that optimal dietary E/P was 41.7–50 kJ/g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specifi c growth rate(SGR) increased markedly when E/P decreased from 62.5 kJ/g to 35.7 kJ/g and gross energy was 12.5 kJ/g, and from 75 kJ/g to 42.9 kJ/g when gross energy was 15.0 kJ/g. The optimal gross energy was estimated at 12.5 kJ /g and excess energy decreased food intake and growth. Dietary lipid exhibited an apparent protein-sparing eff ect. Optimal protein decreased from 35% to 25%–30% with an increase in dietary lipid from 3% to 6% without adversely ef fecting growth. Dietary lipid level af fects the optimal dietary E/P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20%–35% and 10–12.5 k J/g, respectively.展开更多
目的和方法稀有鮈鲫(Gobiocypris rarus)作为一种本土特有的小型鱼类,是我国正在标准化的化学品毒性测试生物之一。为评价稀有鮈鲫鱼类急性毒性实验中效应的稳定性和可重复性,研究优化筛选了重铬酸钾和3,4-二氯苯胺两种化学品,分别在单...目的和方法稀有鮈鲫(Gobiocypris rarus)作为一种本土特有的小型鱼类,是我国正在标准化的化学品毒性测试生物之一。为评价稀有鮈鲫鱼类急性毒性实验中效应的稳定性和可重复性,研究优化筛选了重铬酸钾和3,4-二氯苯胺两种化学品,分别在单一实验室内及不同实验室间进行急性毒性验证实验。结果对于来源相同、体长固定的稀有鮈鲫,无论在实验室内还是实验室间,两组化学品的96 h LC50值均在x珋±2s的质量控制范围之内,所有结果体现了很好的稳定性和可重复性。结论根据研究获得的数据可建立一个有效的稳定性和可重复性衡量数据库,用来评估之后其他单个实验的可靠性;稀有鮈鲫作为一种具有潜力的生态毒性测试的生物种类,具有成为标准实验动物的潜能。展开更多
基金Supported by the National Key Technology R&D Program of China(No.2011BAI15B01-41)the National High Technology Research and Development Program of China(863 Program)(No.2012AA06A302)
文摘An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the ef fects of protein to energy ratio on growth, for the rare minnow( Gobiocypris rarus), which are critical to nutrition standardization for model fi sh. Twenty-four diets were formulated to contain three gross energy(10, 12.5, 15 kJ/g), four protein(20%, 25%, 30%, 35%), and two lipid levels(3%, 6%). The results showed that optimal dietary E/P was 41.7–50 kJ/g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specifi c growth rate(SGR) increased markedly when E/P decreased from 62.5 kJ/g to 35.7 kJ/g and gross energy was 12.5 kJ/g, and from 75 kJ/g to 42.9 kJ/g when gross energy was 15.0 kJ/g. The optimal gross energy was estimated at 12.5 kJ /g and excess energy decreased food intake and growth. Dietary lipid exhibited an apparent protein-sparing eff ect. Optimal protein decreased from 35% to 25%–30% with an increase in dietary lipid from 3% to 6% without adversely ef fecting growth. Dietary lipid level af fects the optimal dietary E/P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20%–35% and 10–12.5 k J/g, respectively.
文摘目的和方法稀有鮈鲫(Gobiocypris rarus)作为一种本土特有的小型鱼类,是我国正在标准化的化学品毒性测试生物之一。为评价稀有鮈鲫鱼类急性毒性实验中效应的稳定性和可重复性,研究优化筛选了重铬酸钾和3,4-二氯苯胺两种化学品,分别在单一实验室内及不同实验室间进行急性毒性验证实验。结果对于来源相同、体长固定的稀有鮈鲫,无论在实验室内还是实验室间,两组化学品的96 h LC50值均在x珋±2s的质量控制范围之内,所有结果体现了很好的稳定性和可重复性。结论根据研究获得的数据可建立一个有效的稳定性和可重复性衡量数据库,用来评估之后其他单个实验的可靠性;稀有鮈鲫作为一种具有潜力的生态毒性测试的生物种类,具有成为标准实验动物的潜能。
