A method for in vitro culture and rapid propagation of Chirita ophio- pogoides was developed using leaves as explants in this study, The results indicat- ed that the medium MS＋6-BA 0.1 mg/L＋NAA 0.1 mg/L was suitable...A method for in vitro culture and rapid propagation of Chirita ophio- pogoides was developed using leaves as explants in this study, The results indicat- ed that the medium MS＋6-BA 0.1 mg/L＋NAA 0.1 mg/L was suitable for bud induc- tion and seedling regeneration from leaves in primary culture. The media MS＋0.5 mg/L 6-BA＋0,1 mg/L NAA＋10% banana＋5% potato and MS＋0.5 mg/L 6-BA＋0.5 mg/L NAA＋2% banana were very suitable for callus multiplication and seedling hardening in subculture, and the proliferation coefficients were 7,9 and 5.6 per 60 d respec- tively. The optimal rooting medium was MS and the rooting rate was 100% on day 30 of culture. The rooted plantlets of C. ophiopogoides were transplanted in green- house with humus soil and 92.5% survived. Theoretically, using the rapid propaga- tion system, about 20 176 seedlings can be reproduced from a sterile plantlet in a year.展开更多
A new flavone glycoside, named mahuangchiside, was isolated from the root of Chirita fimbrisepala Hand.-Mazz. The structure was elucidated as 5,4'-dihydroxy-6-methoxyflavone-7-O-beta-D-xylopyranosyl(1-->2)-beta...A new flavone glycoside, named mahuangchiside, was isolated from the root of Chirita fimbrisepala Hand.-Mazz. The structure was elucidated as 5,4'-dihydroxy-6-methoxyflavone-7-O-beta-D-xylopyranosyl(1-->2)-beta-D-xylopyranoside on the basis of spectral analysis (H-1-H-1 COSY. C-13-H-1 HETCOR and HMBC) and chemical evidence.展开更多
报道了浙江省的2个浙江分布新记录种,分别是发现于遂昌九龙山的梵净山石斛兰Dendrobium fanjingshanense Z.H.Tsi ex X.H.Jin et Y.W.Zhang和发现于永嘉龙湾潭蚂蝗七Chirita fimbrisepala Hand.-Mazz.。引证标本存于浙江林学院植物标本...报道了浙江省的2个浙江分布新记录种,分别是发现于遂昌九龙山的梵净山石斛兰Dendrobium fanjingshanense Z.H.Tsi ex X.H.Jin et Y.W.Zhang和发现于永嘉龙湾潭蚂蝗七Chirita fimbrisepala Hand.-Mazz.。引证标本存于浙江林学院植物标本馆(ZJFC)。展开更多
以条叶唇柱苣苔(Chirita ophiopogoides D Fang et W T Wang)的幼嫩叶片、种子为外植体,以MS为基本培养基,建立了条叶唇柱苣苔的组织培养和再生体系。试验结果表明:条叶唇柱苣苔叶片诱导不定芽的最佳培养基为MS+2,4-D0.5 mg/L+IAA 0.5 m...以条叶唇柱苣苔(Chirita ophiopogoides D Fang et W T Wang)的幼嫩叶片、种子为外植体,以MS为基本培养基,建立了条叶唇柱苣苔的组织培养和再生体系。试验结果表明:条叶唇柱苣苔叶片诱导不定芽的最佳培养基为MS+2,4-D0.5 mg/L+IAA 0.5 mg/L+NAA 0.2 mg/L,丛生芽分化的最佳培养基为MS+KT 1.0 mg/L+IAA 3.0 mg/L+NAA 0.5mg/L;MS+NAA 0.5 mg/L+6-BA 1.5 mg/L的培养基最适合继代,MS+IBA 2.0 mg/L+AC 0.5 mg/L培养基的生根效果最好,平均根长为4.67 cm,生根率达100%。展开更多
通过野外观察和繁育系统的实验，对黄花牛耳朵（Chirita lutea Yan Liu et Y．G．Wei）的传粉生物学进行了研究。结果表明，黄花牛耳朵的花期从7月初至8月底，单株花期约35～47d，单花花期约6～10d，花的开放无固定的时间。在花期内花...通过野外观察和繁育系统的实验，对黄花牛耳朵（Chirita lutea Yan Liu et Y．G．Wei）的传粉生物学进行了研究。结果表明，黄花牛耳朵的花期从7月初至8月底，单株花期约35～47d，单花花期约6～10d，花的开放无固定的时间。在花期内花粉活性约80％，柱头可授性约75％90％。花粉／胚珠比率（P／O）为1215．734-266．13。柱头在花药散粉时已生长至花筒口部，明显高于花药，便于接受异花花粉。黄花牛耳朵不存在无融合生殖，高度自交亲和，但较难发生自动的白花授粉，产生种子主要依靠传粉媒介。自然授粉的结实率明显低于人工授粉的结实率，存在传粉限制，蜜蜂（Apidae sp．）、方头泥蜂（Crabro sp．）、无垫蜂似megilla sp．）是主要的传粉者。展开更多
基金Supported by National Natural Science Foundation of China(31160055)Key Science and Technology Research and Development Program of Guangxi(Gui Ke Gong0992003B-31)~~
文摘A method for in vitro culture and rapid propagation of Chirita ophio- pogoides was developed using leaves as explants in this study, The results indicat- ed that the medium MS＋6-BA 0.1 mg/L＋NAA 0.1 mg/L was suitable for bud induc- tion and seedling regeneration from leaves in primary culture. The media MS＋0.5 mg/L 6-BA＋0,1 mg/L NAA＋10% banana＋5% potato and MS＋0.5 mg/L 6-BA＋0.5 mg/L NAA＋2% banana were very suitable for callus multiplication and seedling hardening in subculture, and the proliferation coefficients were 7,9 and 5.6 per 60 d respec- tively. The optimal rooting medium was MS and the rooting rate was 100% on day 30 of culture. The rooted plantlets of C. ophiopogoides were transplanted in green- house with humus soil and 92.5% survived. Theoretically, using the rapid propaga- tion system, about 20 176 seedlings can be reproduced from a sterile plantlet in a year.
文摘A new flavone glycoside, named mahuangchiside, was isolated from the root of Chirita fimbrisepala Hand.-Mazz. The structure was elucidated as 5,4'-dihydroxy-6-methoxyflavone-7-O-beta-D-xylopyranosyl(1-->2)-beta-D-xylopyranoside on the basis of spectral analysis (H-1-H-1 COSY. C-13-H-1 HETCOR and HMBC) and chemical evidence.
文摘报道了浙江省的2个浙江分布新记录种,分别是发现于遂昌九龙山的梵净山石斛兰Dendrobium fanjingshanense Z.H.Tsi ex X.H.Jin et Y.W.Zhang和发现于永嘉龙湾潭蚂蝗七Chirita fimbrisepala Hand.-Mazz.。引证标本存于浙江林学院植物标本馆(ZJFC)。
文摘通过野外观察和繁育系统的实验，对黄花牛耳朵（Chirita lutea Yan Liu et Y．G．Wei）的传粉生物学进行了研究。结果表明，黄花牛耳朵的花期从7月初至8月底，单株花期约35～47d，单花花期约6～10d，花的开放无固定的时间。在花期内花粉活性约80％，柱头可授性约75％90％。花粉／胚珠比率（P／O）为1215．734-266．13。柱头在花药散粉时已生长至花筒口部，明显高于花药，便于接受异花花粉。黄花牛耳朵不存在无融合生殖，高度自交亲和，但较难发生自动的白花授粉，产生种子主要依靠传粉媒介。自然授粉的结实率明显低于人工授粉的结实率，存在传粉限制，蜜蜂（Apidae sp．）、方头泥蜂（Crabro sp．）、无垫蜂似megilla sp．）是主要的传粉者。
