The Study of Chlamydia Pneumoniae DNA in the Peripheral Blood Mononuclear Cell of Coronary Heart Disease

Objectives To detection of chlamydia pneumoniae (Cpn) DNA in the circulating mononuclear cell fractions of coronary heart disease and to investigate the association between infection with chlamydia pneumoniae and coronary heart disease (CHD) and prospectively whether blood - based nes- ted polymerase chain reaction(nPCR ) is useful in i- dentifying Cpn infection. Methods The peripheral blood mononuclear cell (PBMC) Cpn DNA was exam- ined using nPCR technique and confirmed by electro- phoresis in 150 patients with CHD. Select 55 patients with clinical suspected CHD but angiography result are normal as control group (CG). Then we conducted a prospective , randomized, double - blind, placebo - controlled study of 6 months of azithromycin and place- bo treatment in CHD group. Patients with Cpn DNA positive were then randomized to receive azithromycin or placebo. After treatment blood sample were collect- ed for repeated measurement . Results Chlamydia pneumoniae DNA was detected in 49(32. 7% ) of 150 persons with CHD and in 1 (1. 8% ) of 55 persons with control group,odds ratio 26. 2, 95% confidence interva 13. 52 - 194. 98. The positivity rates of nPCR in CHD groups were higher than those in control group. 16 ca- ses (29. 1%) in latent coronary heart diseases (LCHD)group , 19 cases (39.6%) in unstable angi- na(UAP) group ,and 14 cases (29.9% ) in acute my- ocardial infarction (AMI) group were Cpn positive by nPCR. There were no significant difference among in AMI^UAP and LCHD group. There were significiantdifference in Cpn DNA negative rates after the azithro- mycin and the placebo treatment. Conclusions Chlamydia pneumoniae is present in PBMC of a signifi- cant proportion of persons with CHD. The potential role of chlamydia pneumoniae in coronary atherosclerosis may therefore be more related to acceleration of disease or systemic effects by persistent infection than to sud- den initiation of progressive coronary artery disease by acute infection. The detection of Cpn DNA in PBMC with nPCR may be of great value for identifying Cpn carriers and for monitoring antichlamydial therapy.

The Role of Chlamydia pneumoniae in the Atherosclerotic Process of Patients under 50 Years of Age Who Underwent Coronary Artery Bypass Graft Surgery

Background: To evaluate the presence of C. pneumoniae DNA in the tissues and C. pneumoniae DNA antibodies in the blood samples of patients who underwent CABG surgery. Material and Methods: Fifty-one patients aorta. C. pneumoniae DNA was evaluated in the tissues collected from the atrium, left internal thoracic artery and ascending aorta of patients. Results: Although, C. pneumoniae DNA was negative in the atrial and left internal thoracic artery tissues of all patients, it was positive in the tissues obtained from the ascending aortas of twelve patients. C. pneumoniae DNA positivity was significantly higher in patients with increased aortic intimal thickness compared to those without increased aortic thickness. Conclusion: The question whether C. pneumoniae is triggering atherosclerosis or is involved as a super-infection could not be clarified.

Correlation of Chlamydia pneumonias infection with primary biliary cirrhosis

AIM: To evaluate the association between Chlamydia pneumoniae (Cpn) infection and primary biliary cirrhosis (PBC).METHODS: Cpn IgG and IgM were determined by enzymelinked immunosorbent assay (ELISA) in 41 well-established PBC patients and two race-matched control groups (posthepatitis cirrhosis, n = 70; healthy controls, n = 57).RESULTS: The mean level and seroprevalence of Cpn IgG in PBC group and post-hepatitis cirrhosis (PHC) group were significantly higher than those in healthy controls (46.8±43.4 RU/mL, 49.5±45.2 RU/mL vs28.3±32.7 RU/mL;68.3%, 71.4%, 42.1%, respectively; P＜0.05). There was a remarkably elevated seroprevalence of Cpn IgM in patients with PBC (22.0%) compared to the PHC and healthy control (HC) groups. For the PBC patients versus the HCs, the odds ratios (ORs) of the presence of Cpn IgG and IgM were 2.7 (95% CI 0.9-6.1) and 5.1 (95% CI 1.4-18.5), respectively. Though there was no correlation in the level of Cpn IgG with total IgG in sera of patients with PBC (r = -0.857, P = 0.344＞0.05), Cpn IgM was related with the abnormally high concentrations of total IgM in PBC group.CONCLUSION: The results of this study do not support the hypothesis that infection with Chlamydia pneumoniae may be a triggering agent or even a causative agent in PBC, but suggest that Chlamydia pneumoniae infection probably contributes to the high level of IgM present in most patients with PBC.

Chlamydia pneumoniae replicates in Kupffer cells in mouse model of liver infection

AIM: To develop an animal model of liver infection with Chlamydia pneumoniae (C. pneumoniae) in intraperito-neally infected mice for studying the presence of chlamy-diae in Kupffer cells and hepatocytes.METHODS: A total of 80 BALB/c mice were inoculated intraperitoneally with C. pneumoniae and sacrificed at various time points after infection. Chlamydiae were looked for in liver homogenates as well as in Kupffer cells and hepatocytes separated by liver perfusion with collagenase. C. pneumoniae was detected by both isola-tion in LLC-MK2 cells and fluorescence in situ hybridiza-tion (FISH). The releasing of TNFA-α by C. pneumoniae in vitro stimulated Kupffer cells was studied by enzyme-linked immunosorbent assay.RESULTS: C. pneumoniae isolation from liver homoge-nates reached a plateau on d 7 after infection when 6 of 10 animals were positive, then decreased, and became negative by d 20. C. pneumoniae isolation from sepa-rated Kupffer cells reached a plateau on d 7 when 5 of 10 animals were positive, and became negative by d 20. The detection of C. pneumoniae in separated Kupffer cells by FISH, confirmed the results obtained by culture. Isolated hepatocytes were always negative. Stimula-tion of Kupffer cells by alive C. pneumoniae elicited high TNF-α levels. CONCLUSION: A productive infection by C. pneumo-niae may take place in Kupffer cells and C. pneumoniae induces a local pro-inflammatory activity. C. pneumoniae is therefore, able to act as antigenic stimulus when local-ized in the liver. One could speculate that C. pneumoniaeinfection, involving cells of the innate immunity such as Kupffer cells, could also trigger pathological immune re-actions involving the liver, as observed in human patients with primary biliary cirrhosis.

Relationship between Chlamydia pneumoniae infection and occurrence of bronchial asthma

Objective: To study the relationship between Chlamydia pneumoniae (C. pneumoniae) infection and asthma exacerbation. Methods: A prospective study of C. pneumoniae infection was conducted in 75 patients with asthma and 63 patients with respiratory tract infection, and 100 blood donors served as controls. The presence of infection was convinced by the polymerase chain reaction and direct immunofluorescence assay for C. pneumoniae DNA from throat swab specimens and micro-immunofluorescence testing for C. pneu-moniae-specific IgG, IgM and IgA antibodies. Results: Prevalence of specific IgG in asthma patients (81. 3%) was higher than that of the blood donors (68. 0%, P<0. 05) and was not significantly different from respiratory tract infection patients (68. 0%, P>0. 05). The acute C. pneumoniae infection rate of symptomatic asthma patients (59. 4%) was markedly higher than that of respiratory tract infection patients (34. 9% , P<0. 05). The average titer of C. pneumoniae IgG instead of IgA in asthma patients (48.

Detection of Chlamydia pneumonia DNA in nasopharyngolaryngeal swab samples from patients with rhinitis and pharyngolaryngitis with polymerase chain reaction

Objective To assess the prevalence of Chlamydia pneumomia DNA in patients with otolaryngic disease Methods PCR assay was used to detect Chlamydia pneumonia specific Pst Ⅰ 474 fragment DNA in swabs from patients with acute or subacute pharyngolaryngitis or rhinitis and sinusitis C pneumonia specific antibodies in sera were also assayed with microimmuno fluoresence (MIF) Results About 28% (49/175) of the patients were PCR positive and 25 7%(45/175) were MIF antibodies positive The accordance rate of the two methods was 91 8% Conclusion It is suggested that the C pneumonia infection was common in this group of patients and the C pneumonia Pst Ⅰ474 specific PCR was sensitive and specific for detecting C pneumonia in pharyngolaryngitis or rhinitis and sinusitis

The pathogenesis of Chlamydia pneumoniae-type pneumonitis in mice

Objective To evaluate mice as experimental animals for Chlamydia pneumoniae (C.pneumoniae) infection and investigate the pathogenesis of C.pneumoniae derived pneumonitis.Methods Icr mice were inoculated with the C.pneumoniae strain, CWL-029, either intranasally or intravenously. After a single dose inoculation, mice were killed on the 1st, 3rd, 7th, 14th, 21st, 28th and 60th days. The pathological changes in lung tissue were analyzed.Results The Icr mice were shown to be susceptible to C.pneumoniae. Inoculation into mice with C.pneumoniae induced a prolonged course of lung infection, as demonstrated by persistence of lung pathology (up to 60 days). Via intranasal inoculation of mice, lung pathology was characterized by patchy interstitial pneumonitis with predominately neutrophil leukocyte infiltration early (within the first 7 days) and lymphocyte infiltration in the later stages (14 days later) of infection. After intravenous inoculation, a similarly developed interstitial pneumonitis was observed, but it was milder and patchier, especially in early stages. C.pneumoniae DNA was detected by polymerase chain reaction (PCR) intermittently in the lung tissue. Inoculated mice developed serum IgG antibody responses.Conclusion The Icr mice were susceptible to C.pneumoniae, resulting in a pulmonary infection characterized by interstitial pneumonitis, occurring most strongly via intranasal inoculation.

流产衣原体非典型重症肺炎的诊断及临床特点

目的 探讨流产衣原体非典型重症肺炎的诊断及临床特点。方法 收集2021年1月—2022年11月某院收治并诊断为流产衣原体非典型重症肺炎4例患者的临床资料,全面分析该疾病的临床特征、诊治要点及注意事项。结果 4例患者均为男性,年龄63~73岁,急性起病,均表现为高热、咳嗽、咳痰;3例患者有家禽接触史,1例有流产山羊接触史。4例患者从有临床症状到出现急性呼吸衰竭的间隔时长为1~6 d,且入院时氧合指数(PaO_(2)/FiO_(2))均<200 mmHg,随病情发展呈进行性下降,需积极呼吸机支持。2例患者白细胞计数升高,4例中性粒细胞百分比升高,3例血小板计数轻度降低;4例患者中2例患者天冬氨酸转氨酶升高,2例患者丙氨酸转氨酶升高,3例患者肌酸激酶升高,4例患者血清肌酐均升高,2例轻度低钠血症;4例C反应蛋白、降钙素原、白介素-6明显升高。4例患者胸部CT表现以单个或多个肺叶累及为主,渗出实变,后期累及多个肺叶。支气管肺泡灌洗液宏基因组二代测序均检测出流产衣原体DNA序列。结合4例患者的临床表现、接触史、胸部CT、宏基因组二代测序等结果,诊断为流产衣原体非典型重症肺炎,通过及时调整以多西环素为基础的抗感染治疗方案后,患者病情好转出院。结论 流产衣原体也可能导致人类肺炎发生,感染后可导致严重临床后果,当患者有动物接触史时,需警惕此类疾病;宏基因组二代测序可以检出流产衣原体。

冠心病经皮冠状动脉介入术治疗前后患者血清肺炎衣原体抗体IgA、超敏C-反应蛋白、血管生成素-2变化及预后预测价值研究

目的:探讨冠心病(CHD)经皮冠状动脉介入术治疗前后患者血清肺炎衣原体抗体IgA(CP-IgA)、超敏C-反应蛋白(hs-CRP)及血管生成素-2(Ang-2)变化及预后预测价值。方法:选取CHD患者135例为观察组,根据患者预后情况分为预后良好组(119例)及预后不良组(16例)。另选取同期体检健康者135例为对照组。比较观察组和对照组、观察组PCI前后以及两个亚组血清CP-IgA、hs-CRP及Ang-2水平。分析血清CP-IgA、hs-CRP及Ang-2与PCI术后CHD患者预后的相关性。分析血清CP-IgA、 hs-CRP及Ang-2对PCI术后CHD患者预后的预测价值。结果:观察组血清CP-IgA、hs-CRP及Ang-2水平高于对照组(均P<0.05)。观察组患者PCI术后血清CP-IgA及Ang-2水平较PCI术前降低,血清hs-CRP水平较PCI术前升高(均P<0.05)。预后良好组患者血清CP-IgA、hs-CRP及Ang-2水平低于预后不良组(均P<0.05)。血清CP-IgA、hs-CRP及Ang-2与PCI术后CHD患者预后呈正相关(均P<0.05)。血清CP-IgA、hs-CRP及Ang-2单独预测PCI术后CHD患者预后的曲线下面积(AUC)小于三项联合(均P<0.05)。结论:CHD患者血清CP-IgA、hs-CRP及Ang-2水平升高,PCI术后血清CP-IgA和Ang-2水平降低,hs-CRP水平升高。预后不良患者血清CP-IgA、hs-CRP及Ang-2水平升高,且三者联合对PCI术后CHD患者预后的预测价值较高。

鹦鹉热衣原体肺炎的胸部CT影像表现

目的:探讨鹦鹉热衣原体肺炎的胸部CT影像表现。方法:回顾性分析22例鹦鹉热衣原体肺炎患者的临床及胸部CT资料,分析肺内病变的影像学特点及肺外影像表现。结果:22例鹦鹉热衣原体肺炎中,15例肺内病变表现为大叶性肺炎,5例表现为斑片结节,2例表现为支气管肺炎,常见伴随征象包括:小叶间隔增厚(12例)、小叶内网状影(11例)、铺路石征(6例),少数可见晕征(3例)、反晕征(1例)、小叶中心性结节(1例)、支气管壁增厚(2例)、支气管牵引性扩张(1例)。所有病变均未见坏死或空洞。肺内病变分布:累及单肺19例,双肺3例;累及上肺区6例,下肺区13例,上、下肺区同时受累3例;外周分布12例,支气管血管周围分布4例,弥漫分布6例。10例合并胸腔积液,3例伴纵隔淋巴结增大。结论:鹦鹉热衣原体肺炎的影像表现以大叶性肺炎最多见,典型者表现为斑片状实变伴空气支气管征,多合并磨玻璃密度影,常伴小叶间隔增厚、小叶内网状影及铺路石征等间质受累表现,可见胸腔积液、纵隔淋巴结增大。

鹦鹉热衣原体肺炎的CT表现特征

目的探讨鹦鹉热衣原体肺炎患者的CT表现特征。方法回顾性分析笔者医院经宏基因二代测序(macrogene second-generation sequencing,m NGS)技术确诊10例鹦鹉热衣原体肺炎患者的胸部CT图像。结果7例(70%)患者与鸟(禽)类或其粪便有接触史。入院胸部CT均表现为团片状渗出实变影,均位于单肺,其中累及单叶8例(80%)、双叶2例(20%);10例(100%)均伴有支气管充气征,9例(90%)有磨玻璃影,8例(80%)局部小叶间隔或小叶内间隔增厚;1例(10%)病灶呈“扇形”改变,1例(10%)病灶见“反晕症”,9d内复查9例(90%)有进展,表现为病灶融合增大呈更大范围实变或新出现病灶。结论鹦鹉热衣原体肺炎CT表现具有一定特征,结合鸟(禽)类接触史可提示诊断。

1例鹦鹉热衣原体重症肺炎患者的抗感染治疗及药学监护

目的为鹦鹉热衣原体重症肺炎的抗感染药物治疗及药学监护提供思路和用药参考。方法临床药师参与1例鹦鹉热衣原体重症肺炎患者治疗的全过程,根据患者病史、临床症状、检验结果等情况,协助临床医师动态调整抗感染药物治疗方案:针对鹦鹉热衣原体感染,给予患者替加环素联合阿奇霉素治疗,并结合病原体检查结果对治疗其他感染的用药方案进行动态调整。在治疗过程中,患者出现了QTc间期延长和脂肪酶、淀粉酶升高的可疑药物不良反应,临床药师进行了药学监护并提出合理建议。结果临床医师采纳临床药师建议,患者经治疗后好转出院。结论治疗鹦鹉热衣原体重症肺炎,应兼顾患者、药物、病原体三者的特点个性化制定抗感染药物治疗方案。四环素类抗生素和大环内酯类抗生素对鹦鹉热衣原体感染有明确疗效,但是在临床应用中需关注药物可能引起的不良反应。

四川地区24例鹦鹉热衣原体肺炎的临床特点及治疗分析

目的分析四川地区鹦鹉热衣原体肺炎患者的临床特征及治疗方案,为临床诊治提供参考。方法对成都市第二人民医院2020年12月至2023年2月收治的通过宏基因二代测序(mNGS)确诊的24例鹦鹉热衣原体肺炎患者进行回顾性分析。结果患者主要以发热为首要表现,其他临床症状包括畏寒、寒战、头晕、头痛、乏力、咳嗽、咳痰、肌肉酸痛等,主要并发症为急性呼吸衰竭和肝功能不全,胸部CT多呈单侧肺叶炎性改变,一半以上患者出现胸腔积液。采用多西环素、氟喹诺酮类单药或联合用药的方案进行抗感染治疗后预后良好,联合用药在退热时间上优于单药治疗,但是不能缩短住院时间和用药疗程。结论鹦鹉热衣原体肺炎患者多有鸟类接触史。对不明原因社区获得性肺炎患者应仔细询问其流行病学史,同时结合患者的临床表现、实验室检查和影像学检查对疑似鹦鹉热的患者尽早通过mNGS技术进行辅助诊断。多西环素和氟喹诺酮类药物在鹦鹉热衣原体肺炎患者的治疗中有效,联合用药能使患者体温更快恢复正常。

重症鹦鹉热衣原体肺炎1例

鹦鹉热衣原体肺炎是一种较为少见的人畜共患病,肺部病变重,多合并急性呼吸衰竭及多器官功能受损,因缺乏特发性,常被误诊或漏诊。该文对1例鹦鹉热衣原体肺炎的诊疗进行报道。

二代测序技术诊断鹦鹉热衣原体肺炎8例

目的探索鹦鹉热衣原体肺炎的临床表现、实验室检查、影像学表现、治疗及随访情况。方法回顾性分析2021年1月至2022年8月经mNGS确诊为鹦鹉热衣原体肺炎的8例患者的临床资料。结果8例患者中男1例,女7例,年龄为33~81岁。4例有鸟类/家禽的接触史。临床表现主要为高热(8/8)、咳嗽(8/8)、咳痰(4/8)、神经系统症状(3/8)。实验室检查提示白细胞升高(4/8)、中性粒细胞比例均升高(8/8)、淋巴细胞比例均降低(8/8);所有患者的C反应蛋白、降钙素原明显升高伴有低蛋白血症(8/8);谷丙转氨酶(8/8)、谷草转氨酶(8/8)、肌酸激酶(4/8)、乳酸脱氢酶(6/8)升高;胸部影像学多表现为斑片状渗出实变(7/8)、团块状实变(1/8),累及单侧居多(7/8)。所有患者均由肺泡灌洗液mNGS确诊,确诊后调整为多西环素(4/8)或奥玛环素(2/8)或莫西沙星(2/8),所有患者均治愈出院。结论鹦鹉热衣原体肺炎有一定的特征性,但临床诊断仍然困难。mNGS检测快、准确性高,有助于早期明确诊断、尽早选用敏感抗菌药物、改善预后。

51例社区获得性鹦鹉热衣原体肺炎的临床影像学与疗效分析

目的探讨鹦鹉热衣原体肺炎的临床影像学特点,从而指导早期诊断及治疗,改善预后。方法回顾性分析我院2019年1月至2023年4月间通过宏基因组二代测序诊断的51例鹦鹉热衣原体肺炎住院患者资料。结果51例患者主要症状为高热(47/51,92.16%)、咳嗽(35/51,68.63%)、咳痰(24/51,47.06%)、胸闷(18/51,35.29%)、呼吸衰竭(17/51,33.33%)。外周血中白细胞正常或减低(38/51,74.50%)、CRP升高(51/51,100%)、转氨酶升高(34/51,66.67%)、D-二聚体增高(48/51,94.12%)。影像学主要表现为:肺实变(46/51,90.20%)、磨玻璃样改变(15/51,29.41%)、胸腔积液(23/51,45.10%)、多叶病变(13/51,25.50%)。治疗:单药治疗(四环素类、喹诺酮类或大环内酯类)27例(52.94%),总有效率为88.89%;联合治疗(喹诺酮类联合四环素类或大环内酯类)22例(43.14%),总有效率90.90%。结论鹦鹉热衣原体肺炎以高热、咳嗽伴肺实变、磨玻璃样改变为特点,应尽早诊断加用针对性抗生素治疗,重症患者建议早期联合治疗。

鹦鹉热衣原体肺炎8例临床诊治及文献复习

目的探讨肺泡灌洗液宏基因组二代测序技术(mNGS)对鹦鹉热衣原体肺炎的诊断情况,以加深对该病的诊治认识。方法选取2020年9月至2023年9月于我院住院且经肺泡灌洗液mNGS确诊为鹦鹉热支原体肺炎的8例患者。统计分析患者的一般资料、实验室、胸部CT、支气管镜、肺泡灌洗液mNGS检查结果及治疗经过、治疗结局。结果8例患者中位年龄56(55,57)岁;临床表现为发热、咳嗽、气喘、乏力。所有患者中性粒细胞百分比及C反应蛋白(CRP)、D-二聚体(D-D)水平均升高,4例患者降钙素原(PCT)水平升高,5例患者合并呼吸衰竭,3例患者为重症肺炎。8例患者的影像学表现主要为实变影伴或不伴磨玻璃影,1例表现为单纯性磨玻璃影,4例合并胸腔积液。所有患者确诊后均接受以多西环素为基础的抗感染治疗且均好转出院,出院后复查胸部CT显示吸收好转。结论鹦鹉热衣原体肺炎具有一定的特征性,以高热多见,实验室检查通常存在中性粒细胞百分比及CRP升高,影像学主要表现为实变影,可快速进展出现呼吸衰竭,甚至重症肺炎,经针对性抗感染及支持治疗,通常预后良好。

鹦鹉热衣原体肺炎病例报告2例及文献复习

本文报道2例患者均有鸽子接触史,表现为发热、乏力、呼吸困难,1例患者病情进展较快,于发病第4天出现急性呼吸窘迫综合征,需无创呼吸机辅助通气。2例患者白细胞计数轻度升高,淋巴细胞百分比降低,血小板计数正常,降钙素原升高,C反应蛋白明显升高,丙氨酸氨基转移酶及天门冬氨酸氨基转移酶不同程度升高,血清钠、钾、白蛋白降低,1例红细胞沉降率(ESR)正常,1例轻度升高,血、肺泡灌洗液常规培养及血清学检查未能明确病原体。胸部X射线、计算机断层成像(CT)表现为一侧肺叶大片渗出和实变,严重者进展快,累及双侧肺叶,出现呼吸衰竭,经验性使用抗生素效果不佳。及时采用宏基因组二代测序(mNGS)技术并结合鸟类接触史,最终确诊为鹦鹉热衣原体肺炎,采用喹诺酮类联合四环素类抗生素治疗后,患者症状、体征和影像学异常均明显好转,但1例患者影像学吸收不佳,进一步联合大环内酯类抗生素治疗1周,随访复查胸部CT,病灶基本吸收。通过分析该2例患者的临床特点、诊断及治疗过程,为临床诊治该类疾病提供参考。

C-X-C趋化因子受体4增强Toll样受体2在肺炎衣原体感染促进动脉粥样硬化病变形成中的作用

[目的]探究C-X-C趋化因子受体4(CXCR4)在肺炎衣原体(C.pn)感染促进动脉粥样硬化(As)病变形成中的作用。[方法]以高脂饮食为基础,建立C.pn感染诱导ApoE^(-/-)、ApoE^(-/-)+Toll样受体2(TLR2)^(-/-)、ApoE^(-/-)+TLR2^(-/-)+AMD3100小鼠As模型,ELISA检测ApoE^(-/-)小鼠血清C.pn IgG、IgM抗体水平,PCR检测肺组织C.pn特异性DNA,油红O染色和HE染色观察主动脉及主动脉根部脂质沉积和As病变面积,比色法测定血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDLC)和高密度脂蛋白胆固醇(HDLC)水平,ELISA检测血清白细胞介素1β(IL-1β)和白细胞介素6(IL-6)含量。[结果]ApoE^(-/-)小鼠C.pn感染模型成功建立。与对照组相比,C.pn感染后ApoE^(-/-)小鼠主动脉及主动脉根部脂质沉积量增加89.08%和71.83%,As病变面积增加34.12%(均P<0.05);与C.pn感染组相比,TLR2^(-/-)+C.pn感染组主动脉及主动脉根部脂质沉积量减少46.16%和75.73%,As病变面积减少63.37%(均P<0.05);与TLR2^(-/-)+C.pn感染组相比,TLR2^(-/-)+AMD3100+C.pn感染组主动脉及主动脉根部脂质沉积量减少26.19%和56.94%,As病变面积则减少22.24%(均P<0.05)。与对照组相比,C.pn感染后血清TC、TG和LDLC水平分别升高0.62倍、1.43倍和1.34倍,血清IL-1β和IL-6含量分别增加4.10倍和6.00倍(均P<0.05);与C.pn感染组相比,TLR2^(-/-)+C.pn感染组血清TC、TG和LDLC水平分别降低56.96%、50.41%和66.64%,血清IL-1β和IL-6含量分别减少66.72%和69.54%(均P<0.05);与TLR2^(-/-)+C.pn感染组相比,TLR2^(-/-)+AMD3100+C.pn感染组血清TC、TG和LDLC水平分别降低52.18%、58.56%和60.61%,血清IL-1β和IL-6含量分别减少28.84%和43.18%(均P<0.05)。[结论]CXCR4可增强TLR2在升高血脂水平及炎症因子含量中的作用,进而参与C.pn感染诱导的As病变形成。

1例鹦鹉热衣原体肺炎患者的中西医结合护理体会

本文总结1例鹦鹉热衣原体肺炎患者的中西医结合护理经验。基于护理评估和中医辨证施护,在饮食护理、体温护理、情志护理等常规干预基础上,给予耳穴压丸调理脏腑、穴位贴敷止咳中医护理适宜技术,有效控制肺部感染,提高患者免疫力,促进患者康复。