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Distribution properties of airflow in the full envelope type maize stalk chopping chamber
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作者 Peng Liu Jin He +4 位作者 Changchang Yu Shangyi Lou Chunlei Wang Mingxu Wang Huipeng Shen 《International Journal of Agricultural and Biological Engineering》 SCIE 2024年第5期137-150,共14页
Chopped and spread maize stalks improve soil structure and fertility. However, because of the absence of research on airflow distribution in the chopping chamber, improvement of the spreading uniformity of chopped sta... Chopped and spread maize stalks improve soil structure and fertility. However, because of the absence of research on airflow distribution in the chopping chamber, improvement of the spreading uniformity of chopped stalks has been limited. Therefore, in this study, computational fluid dynamics (CFD) technology was applied to analyze the influence of structural and operational parameters of the chopping and spreading machine on the velocity, pressure, and turbulent kinetic energy distribution of airflow in the chopping chamber. The experimental factors considered were the relative position angle (RPA) between the collecting-chopping shaft and the sliding-supporting shaft, working velocity (WV) of the chopping chamber, and rotational velocity of the collecting-chopping blade (RVCCB). The results revealed that RPA and RVCCB had a significant influence on the maximum negative pressure in the inlet (MNPI), the proportion of negative pressure area at inlet (PNPAI), and the maximum pressure drop at inlet and outlet (MPDIO). Additionally, RVCCB had a strong influence on the maximum velocity, average velocity, and velocity variation coefficient of airflow at the outlet. Moreover, maximum turbulence (MT) and maximum turbulent kinetic energy dissipation rate (MTKEDR) showed a positive relationship with RVCCB. To determine the values of RPA, RVCCB, and WV, a multivariate parameters optimization regression model was constructed, which yielded the optimal values of 15°, 1800 r/min, and 0.50 m/s, respectively. Subsequently, a hyperbolic spiral-type guiding shell with an arc length of 90° was designed to enhance the uniform distribution of airflow in the chopping chamber. Finally, a validation experiment of airflow distribution was conducted. The results showed that the velocity difference between the simulation and the validation experiment was less than 15%, indicating the accuracy of CFD simulation, and the spreading uniformities of the chopped stalks were better than national standards. These findings can serve as technical and theoretical support for the design and optimization of chopping and spreading machines. 展开更多
关键词 maize stalk retention conservation tillage spreading process airflow distribution full envelope-type chopping chamber
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一种Java程序Chopping方法 被引量:2
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作者 蒋曹清 应时 +1 位作者 倪友聪 管华 《计算机科学》 CSCD 北大核心 2011年第1期150-155,161,共7页
程序Chopping对于程序理解、分析、调试、测试等具有重要的意义。已有的Chopping方法主要基于相互连接的系统依赖图(SDG),对于大程序这种SDG描述通常非常复杂,易导致程序Chopping结果不准确。针对这一问题,基于带标签的Java程序描述方法... 程序Chopping对于程序理解、分析、调试、测试等具有重要的意义。已有的Chopping方法主要基于相互连接的系统依赖图(SDG),对于大程序这种SDG描述通常非常复杂,易导致程序Chopping结果不准确。针对这一问题,基于带标签的Java程序描述方法,提出一种Java程序Chopping方法。该方法利用这种描述附带的程序依赖信息,分析参数依赖关系,并在此基础上给出Chopping算法。此程序Chopping方法能把Java程序方法间的程序Chop-ping问题转换到Java程序方法内进行分析,程序依赖图具有结点少、可重用、可并发构造等优点。最后通过实例和实验给出程序Chopping方法的实施过程及有效性。 展开更多
关键词 程序chopping chopping算法 JAVA程序 chopping方法
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Chopping-peaking开关形成高功率超宽带双极脉冲的实验研究 被引量:12
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作者 樊亚军 石磊 +4 位作者 刘国治 汪文秉 周金山 刘峰 朱郁丰 《强激光与粒子束》 EI CAS CSCD 北大核心 2004年第4期501-504,共4页
 设计研制了一种chopping peaking组合开关型高功率双极脉冲形成装置,通过调节两个开关的导通时刻来得到不同形状的双极脉冲。理想情况下,形成的双极脉冲电压峰 峰值等于入射脉冲峰值电压的2倍,实验中入射脉冲峰值电压为205kV的单极脉...  设计研制了一种chopping peaking组合开关型高功率双极脉冲形成装置,通过调节两个开关的导通时刻来得到不同形状的双极脉冲。理想情况下,形成的双极脉冲电压峰 峰值等于入射脉冲峰值电压的2倍,实验中入射脉冲峰值电压为205kV的单极脉冲,获得了最大峰 峰电压为360kV的双极脉冲,是入射脉冲峰值电压的1.76倍。双极脉冲的持续时间可以改变,最大值为单极脉冲的脉宽。入射脉冲上升时间越小,得到的双极脉冲峰 峰电压就越大。 展开更多
关键词 超宽带 双极脉冲 陡化 chopping开关 peaking开关
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基于Chopping的Web应用SQL注入漏洞检测方法 被引量:3
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作者 尤枫 马金慧 张雅峰 《计算机系统应用》 2018年第1期86-91,共6页
随着Web应用的不断普及,其安全问题越来越显突出,特别是SQL注入漏洞攻击,给用户的安全体验造成了巨大的威胁.针对二阶SQL注入漏洞,本文提出了一种基于chopping技术的二阶SQL注入漏洞检测方法.首先通过对待测应用程序进行chopping,获取... 随着Web应用的不断普及,其安全问题越来越显突出,特别是SQL注入漏洞攻击,给用户的安全体验造成了巨大的威胁.针对二阶SQL注入漏洞,本文提出了一种基于chopping技术的二阶SQL注入漏洞检测方法.首先通过对待测应用程序进行chopping,获取到一阶SQL注入疑似路径;然后对一阶SQL注入疑似路径中的SQL语句进行分析,确定二阶SQL注入操作对,进而得到二阶SQL注入疑似路径;最后通过构造攻击向量并运行,确认二阶SQL注入疑似路径中漏洞是否实际存在.实验结果表明,本方法能够有效地检测出二阶SQL注入漏洞. 展开更多
关键词 二阶SQL注入 污点分析 WEB应用 漏洞检测 chopping 程序切片
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Efficacy of wet-lab training versus surgical-simulator training on performance of ophthalmology residents during chopping in cataract surgery 被引量:5
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作者 Ya-Guang Hu Qiu-Ping Liu +4 位作者 Ning Gao Chang-Rui Wu Jian Zhang Li Qin Jing-Ming Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2021年第3期366-370,共5页
AIM: To analyze whether wet-lab training(WLT) or surgical-simulator training(SST) is better for ophthalmology residents to master the chopping technique.METHODS: Sixty ophthalmology residents(in their second year) and... AIM: To analyze whether wet-lab training(WLT) or surgical-simulator training(SST) is better for ophthalmology residents to master the chopping technique.METHODS: Sixty ophthalmology residents(in their second year) and three cataract surgeons participated in the study. The residents were randomly separated into two groups, WLT group and SST group. The residents in WLT group were asked to perform 10 trials of chopping using pig eyes and scored by the surgeons, and then they performed and scored using simulator for one time. The residents in SST group underwent 10 trials of chopping using simulator, and the simulator scored each trail. Then, this group were asked to perform the chopping using pig eyes and scored by the surgeons. At last, we investigated the residents’ satisfaction about the training.RESULTS: The demographic characteristics had no significant differences between the two groups. Recorded by the simulator, the residents in SST group got significantly higher overall score(83.90±1.31) than WLT group(78.73±1.92, P=0.03). And the residents in SST group got less corner area injured, and they spend less time than WLT group(P<0.05). Moreover, the residents in WLT group used more ultrasonic energy value than SST group(P=0.03). However, scored by the surgeons, the residents in two groups got nearly the same overall score. The residents in WLT group performed better on the frequencies of posterior capsule torn and incisional stress(P=0.03, 0.008, respectively). In the survey, the residents in two groups held the same opinion that the training was helpful and they strongly recommended this training. And all of them enjoyed the training, and enjoyed being randomized in their own group. However, with respect to the realistic character, the residents thought that WLT was better than SST(P<0.001).CONCLUSION: Both of the Eyesi surgical-stimulator and the wet-lab improve the residents’ chopping ability and each has its own advantages. The combination of the two training ways could be considered to be a part of the training curriculum for new residents. 展开更多
关键词 chopping in cataract simulator wet-lab
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SRG POSITION SENSORLESS TECHNIQUE WITH CURRENT CHOPPING MODE 被引量:2
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作者 ZhuXuezhong LiuDiji +1 位作者 GuiXiaojie LiuChuang 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2002年第1期14-18,共5页
A onvel current chopping strategy for switched reluc-tance generator(SRG)-a full conducted current chopping(FCCC)scheme is presented,According to characteristics lf phase current wave of SRG generating operation,it ca... A onvel current chopping strategy for switched reluc-tance generator(SRG)-a full conducted current chopping(FCCC)scheme is presented,According to characteristics lf phase current wave of SRG generating operation,it can be generated under sensorless condition without an addi-tional circuit or a position signal algorithm.Simulational results show the feasibility of this scheme.Experimental results of a 6kW6/4configuration SRG show its simplic-ity and high reliability with little decrease in efficiency.Soit will be widely used. 展开更多
关键词 SRG position SENSORLESS current chopping control 开关磁阻发电机 无位置传感器 电流斩波控制
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Reduction of current chopping noise with DSP controller in switched reluctance motor drive system
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作者 郭伟 詹琼华 马志源 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2002年第4期419-423,共5页
A novel current chopping mode was used in a switched reluctance motor drive system to make full use of the characteristics of digital signal processor (DSP) TMS320F240. The necessity of this 180° phase-shift curr... A novel current chopping mode was used in a switched reluctance motor drive system to make full use of the characteristics of digital signal processor (DSP) TMS320F240. The necessity of this 180° phase-shift current control (PSCC) mode is introduced first and then the principle of PSCC covering both hardware requirement and software programming is described in detail. The analysis made indicated that with this mode, the chopping frequency in winding can reach 20 kHz with 10 kHz power switches and the control frequency can reach 40 kHz at the same time. Subsequently, based on the linear and nonlinear mathematical models of the switched reluctance motor (SRM), some simulation work has been done. The simulation results show that when this mode is applied to SRM drive (SRD) system, the current waveform becomes better. So the ripple of the torque is reduced simultaneously and the vibration and acoustic noise are reduced involuntarily. Stationary tests show that the acoustic noise is greatly diminished. Finally, some experiments were made using a 50 kW SRD system for electric vehicle (EV). Experimental results indicate that this mode can be implemented feasibly and it has a good action on the SRD system. 展开更多
关键词 SWITCHED reluctance MOTOR current chopping control DSP VIBRATION ACOUSTIC noise INVERTER
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CHOPPING WAVE METHOD FOR REMOVING ELECTROMAGNETIC INDUCTION COUPLING
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作者 He Jishan Wang +1 位作者 Shaowu Tang Jingtian(Central South University of technology , Changsha 410083) 《中国有色金属学会会刊:英文版》 CSCD 1995年第1期1-10,共10页
CHOPPINGWAVEMETHODFORREMOVINGELECTROMAGNETICINDUCTIONCOUPLINGHe;JishanWang;ShaowuTang;Jingtian(CentralSouthU... CHOPPINGWAVEMETHODFORREMOVINGELECTROMAGNETICINDUCTIONCOUPLINGHe;JishanWang;ShaowuTang;Jingtian(CentralSouthUniversityoftechno... 展开更多
关键词 induced polarization effect electromagnetic INDUCTION COUPLING chopping WAVE METHOD DECOUPLING METHOD
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Design of New Farm Machine for Chopping Field Straw,Pressing it into Soil and Cleaning up Plastic Film
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作者 Jiqiang CUI Lele HE 《Asian Agricultural Research》 2017年第3期110-111,共2页
In this paper,we design a farm machine integrating the functions of chopping field straw,pressing field straw into soil,breaking stubble,and cleaning up plastic film,in order to improve the efficiency of plastic film ... In this paper,we design a farm machine integrating the functions of chopping field straw,pressing field straw into soil,breaking stubble,and cleaning up plastic film,in order to improve the efficiency of plastic film cleaning,enrich the function of straw chopping equipment,reduce the noise generated at work,and further realize the intelligent harmony. The remote control button is used to control the work of the entire system,and the bluetooth remote control module and matrix keyboard are used to control the vehicle. With STM32 microcontroller as the main control chip,the machine controls the motion of the electric motor by relay,and employs LCD screen to realize real-time display of system work. It is convenient and easy to operate,with good human-computer interaction. 展开更多
关键词 Field straw chopping Pressing straw into soil Plastic film clean-up MICROCONTROLLER
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Optimization design and experiment on feeding and chopping device of silage maize harvester
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作者 Meizhou Chen Guangfei Xu +5 位作者 Maojian Wei Xiaowei Li Yuanzhen Wei Peisong Diao Peide Cui Shaomin Teng 《International Journal of Agricultural and Biological Engineering》 SCIE 2023年第3期64-77,共14页
Horizontal feeding devices and plate hob chopping devices are the key component of silage maize harvester.To solve the problem of feeding blockage,reduce energy consumption,and improve the chopping quality of the chop... Horizontal feeding devices and plate hob chopping devices are the key component of silage maize harvester.To solve the problem of feeding blockage,reduce energy consumption,and improve the chopping quality of the chopping device a horizontal different diameter five-rollers device(HDDFD)was designed and the plate hob chopping device was simultaneously optimized and analyzed.Through the dynamic analysis,the feeding conveying speed was determined to be 2.0-4.5 m/s.The distance equation of the actual and theoretical cutting-edge curve and the position of the fixed blade were finally obtained.Single factor and response surface orthogonal tests in the bench site were carried out with feeding speed,rotating speed of chopping cylinder,feeding amount,and feeding direction as influencing factors,standard grass length rate(SGLR),and energy consumption per unit mass(ECPUM)as evaluation indexes.The optimal working parameters for chopping performance could be concluded as a feeding speed of 3.39 m/s,rotating speed of the chopping cylinder of 1016.17 r/min,feeding amount of 8.04 kg/s,and feeding direction of 52.2°.In addition,the SGLR and ECPUM were obtained as 95.35%and 37.63 kJ/kg,respectively.The relative error between the experimental results with round parameter combination and the predicted value was verified to be less than 5%.Field tests verified the reliability of the optimized feeding and chopping device.It can be seen that the HDDFD and optimized plate hob chopping device can meet the requirements of mechanized silage harvesting which obviously improves the working quality and reduce the energy consumption of chopping. 展开更多
关键词 silage maize feeding device chopping device stand grass length ratio energy consumption
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温阳生肌膏调控GRP78/CHOP通路抑制过度内质网应激促糖尿病难愈合创面修复 被引量:3
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作者 丁雅容 郑时旭 +4 位作者 王君 谢晨磊 奉水华 周忠志 陈丽 《中国病理生理杂志》 CAS CSCD 北大核心 2024年第3期493-501,共9页
目的:通过分析葡萄糖调节蛋白78(CRP78)/CCAAT/增强子结合蛋白同源蛋白(CHOP)通路研究温阳生肌膏对内质网应激(ERS)的调控,以探讨温阳生肌膏促进糖尿病难愈合创面修复的相关机制。方法:SD大鼠高脂饲料喂养联合链脲霉素腹腔注射后手术制... 目的:通过分析葡萄糖调节蛋白78(CRP78)/CCAAT/增强子结合蛋白同源蛋白(CHOP)通路研究温阳生肌膏对内质网应激(ERS)的调控,以探讨温阳生肌膏促进糖尿病难愈合创面修复的相关机制。方法:SD大鼠高脂饲料喂养联合链脲霉素腹腔注射后手术制备背部全层皮肤缺损建立大鼠糖尿病难愈合创面模型;实验分为正常组、模型组、温阳生肌膏组和贝复新(重组牛碱性成纤维细胞生长因子外用凝胶)组;正常组及模型组消毒后予以生理盐水涂抹,温阳生肌膏组及贝复新组分别以温阳生肌膏、贝复新涂抹创面,每日换药1次;处理14 d后观察创面愈合情况,计算创面愈合率;HE染色观察创面组织显微形态;Western blot法检测创面组织GRP78、CHOP和cas pase-12含量;免疫组化法检测创面GRP78、CHOP和caspase-12表达及分布情况;透射电子显微镜观察创面内质网数量及肿胀情况;ELISA检测创面促炎因子白细胞介素1β(IL-1β)含量。结果:各组相应干预14 d后检测各项指标。与正常组相比,模型组创面愈合率显著降低(P<0.01),炎症渗出较多,肉芽组织生长情况差,GRP78、CHOP和caspase-12蛋白表达升高(P<0.01),炎症因子IL-1β含量显著增加(P<0.01);与模型组相比,温阳生肌膏组创面愈合率显著增加(P<0.01),炎症渗出减少,肉芽组织生长良好,创面组织GRP78、CHOP和caspase-12蛋白表达显著降低(P<0.01);炎症因子IL-1β含量显著降低(P<0.01)。结论:温阳生肌膏促进糖尿病难愈合创面愈合的作用,可能与其下调GRP78/CHOP通路,抑制过度ERS,降低创面细胞凋亡水平有关。 展开更多
关键词 温阳生肌膏 内质网应激 GRP78/CHOP通路 糖尿病难愈合创面
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内质网应激PERK-eIF2α-AFT4-CHOP信号通路在血液肿瘤中的研究进展 被引量:1
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作者 贺梦可 徐子真 李军民 《肿瘤防治研究》 CAS 2024年第2期140-146,共7页
在生理状态下,内质网主要负责蛋白质的生物合成和成熟。然而,当机体稳态受到内外因素干扰破坏后,引发内质网中未折叠和错误折叠蛋白的累积,进而诱导产生内质网应激和未折叠蛋白反应(UPR)。在内质网应激条件下,未折叠蛋白反应可通过不同... 在生理状态下,内质网主要负责蛋白质的生物合成和成熟。然而,当机体稳态受到内外因素干扰破坏后,引发内质网中未折叠和错误折叠蛋白的累积,进而诱导产生内质网应激和未折叠蛋白反应(UPR)。在内质网应激条件下,未折叠蛋白反应可通过不同途径来维持细胞内稳态,其中活化蛋白激酶R样内质网激酶(PERK)是其重要途径之一。活化的PERK使真核翻译起始因子2亚基α(eIF2α)磷酸化,引发活性转录因子4(ATF4)选择性翻译,并与促凋亡转录因子C/EBP同源蛋白(CHOP)的启动子直接结合诱导细胞凋亡。该信号通路也是UPR参与血液肿瘤细胞和免疫细胞调节的重要机制之一。本文阐述了PERK-eIF2α-ATF4-CHOP信号通路在血液肿瘤中的研究进展,以及靶向该信号通路在血液肿瘤治疗中的潜在价值。 展开更多
关键词 内质网应激 未折叠蛋白反应 蛋白激酶R样内质网激酶 EIF2Α ATF4 CHOP 血液肿瘤
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基于PERK/ATF4/CHOP信号通路研究滋阴明目方含药血清对衣霉素诱导的ARPE-19细胞的作用机制 被引量:1
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作者 谢薇 彭俊 +2 位作者 宋厚盼 欧晨 彭清华 《湖南中医药大学学报》 CAS 2024年第5期785-790,共6页
目的研究滋阴明目方含药血清对衣霉素诱导ARPE-19细胞的影响及其可能机制。方法构建细胞内质网应激损伤模型,将ARPE-19细胞分为空白组、模型组、空白血清组、滋阴明目方含药血清组、牛磺熊去氧胆酸组。对细胞进行形态观察,CCK-8检测细... 目的研究滋阴明目方含药血清对衣霉素诱导ARPE-19细胞的影响及其可能机制。方法构建细胞内质网应激损伤模型,将ARPE-19细胞分为空白组、模型组、空白血清组、滋阴明目方含药血清组、牛磺熊去氧胆酸组。对细胞进行形态观察,CCK-8检测细胞存活率,TUNEL法检测细胞凋亡,Western blot检测细胞蛋白激酶样内质网激酶(PERK)、活化转录因子4(ATF4)、C/EBP同源蛋白(CHOP)蛋白的表达。结果选用浓度50μmol/L衣霉素干预ARPE-19细胞造模。观察细胞形态发现,滋阴明目方含药血清组和牛磺熊去氧胆酸组ARPE-19细胞较模型组细胞数量增多,生长较均匀,漂浮的死亡ARPE-19细胞及碎片减少。与空白组相比,模型组和空白血清组的细胞存活率下降(P<0.01),凋亡率明显上升(P<0.01),PERK、ATF4、CHOP蛋白表达上调(P<0.01)。与模型组相比,滋阴明目方含药血清组细胞存活率上升(P<0.01),凋亡率明显下降(P<0.01),PERK、ATF4、CHOP蛋白表达下调(P<0.01)。结论滋阴明目方含药血清可以减少ARPE-19细胞内质网应激损伤模型的凋亡,其分子机制与调控PERK-ATF4-CHOP信号通路有关。 展开更多
关键词 滋阴明目方 ARPE-19细胞 衣霉素 内质网应激损伤模型 PERK/ATF4/CHOP信号通路
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葛根芩连汤对2型糖尿病db/db小鼠胰腺组织PERK/ATF4/CHOP信号通路的影响 被引量:1
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作者 关晓文 梁永林 +3 位作者 朱向东 苏菲 张媛媛 翟艳会 《中国中医药信息杂志》 CAS CSCD 2024年第1期97-103,共7页
目的观察葛根芩连汤对2型糖尿病(T2DM)小鼠胰腺内质网应激的影响,探讨其治疗T2DM的作用机制。方法75只SPF级雄性db/db小鼠随机分为模型组、二甲双胍组和葛根芩连汤高、中、低剂量组,每组15只,15只db/m小鼠作为空白组,给药组分别予相应... 目的观察葛根芩连汤对2型糖尿病(T2DM)小鼠胰腺内质网应激的影响,探讨其治疗T2DM的作用机制。方法75只SPF级雄性db/db小鼠随机分为模型组、二甲双胍组和葛根芩连汤高、中、低剂量组,每组15只,15只db/m小鼠作为空白组,给药组分别予相应药物灌胃12周。检测小鼠体质量、空腹血糖(FBG)及糖化血红蛋白(HbA1c),HE染色观察胰腺组织病理变化,TUNEL染色检测胰岛细胞凋亡情况,Western blot检测胰腺组织葡萄糖调节蛋白78(GRP78)、蛋白激酶R样内质网激酶(PERK)、p-PERK、活化转录因子4(ATF4)、C/EBP同源蛋白(CHOP)蛋白表达,实时荧光定量PCR检测胰腺组织PERK、ATF4、CHOP mRNA表达。结果与空白组比较,模型组小鼠体质量、FBG和HbA1c含量显著增加(P<0.01);胰腺组织结构不完整,边界模糊,内有空泡,胰岛细胞凋亡明显增加(P<0.01);胰腺组织GRP78、p-PERK、ATF4、CHOP蛋白表达显著升高(P<0.01),PERK、ATF4、CHOP mRNA表达显著升高(P<0.01)。与模型组比较,各给药组小鼠体质量、FBG和HbA1c含量显著减少(P<0.05,P<0.01);胰腺组织病理改变有所减轻,胰岛细胞凋亡不同程度减少(P<0.05,P<0.01);葛根芩连汤高、中剂量组和二甲双胍组胰腺组织GRP78、p-PERK、ATF4、CHOP蛋白表达显著降低(P<0.01),PERK、ATF4、CHOP mRNA表达显著降低(P<0.05,P<0.01)。结论葛根芩连汤可能通过抑制内质网应激PERK/ATF4/CHOP信号通路,下调相关基因和蛋白表达,减少胰岛细胞凋亡,保护胰岛细胞功能,延缓T2DM进展。 展开更多
关键词 葛根芩连汤 2型糖尿病 内质网应激 PERK/ATF4/CHOP信号通路 小鼠
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FABP4沉默通过调节PERK/eIF2α/ATF4/CHOP信号通路对妊娠糖尿病大鼠内质网应激和胰岛素抵抗的影响 被引量:1
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作者 李贞 余冬平 +3 位作者 罗武 陶婷 陈辉 王宁 《中国免疫学杂志》 CAS CSCD 北大核心 2024年第4期705-713,共9页
目的:探讨脂肪酸结合蛋白4(FABP4)沉默通过调节蛋白激酶R样内质网激酶(PERK)/真核细胞启动因子2α(eIF2α)/转录因子4(ATF4)/C/EBP同源蛋白(CHOP)信号通路对妊娠糖尿病(GDM)大鼠内质网应激(ERS)和胰岛素抵抗(IR)的影响。方法:腹腔注射... 目的:探讨脂肪酸结合蛋白4(FABP4)沉默通过调节蛋白激酶R样内质网激酶(PERK)/真核细胞启动因子2α(eIF2α)/转录因子4(ATF4)/C/EBP同源蛋白(CHOP)信号通路对妊娠糖尿病(GDM)大鼠内质网应激(ERS)和胰岛素抵抗(IR)的影响。方法:腹腔注射链脲佐菌素(STZ)建立GDM大鼠模型。通过尾静脉注射FABP4 siRNA质粒(si-FABP4)、阴性对照质粒(NC)和PERK激活剂(CCT020312),将大鼠随机分为Normal组、GDM组、GDM+NC组、GDM+si-FABP4组、GDM+si-FABP4+CCT020312组。检测胰腺组织中FABP4含量、血脂水平、炎症标志物水平和氧化应激标志物水平,检测胰腺组织中PERK/eIF2α/ATF4/CHOP信号通路相关蛋白表达。HTR-8/SVneo细胞分为5组:对照(Control)组、高糖(HG)组、HG+NC组、HG+si-FABP4组、HG+si-FABP4+CCT020312组,24 h后检测细胞中FABP4及PERK/eIF2α/ATF4/CHOP信号通路相关蛋白表达。结果:与Normal组相比,GDM组大鼠血清和胰腺组织中FABP4水平显著上调(P<0.05)。FABP4沉默显著降低了FBG和IR,并伴有血脂、CRP、TNF-α、IL-6及MDA水平降低和SOD、CAT水平升高(P<0.05)。此外,FABP4沉默减轻了胰腺和胎盘组织损伤。而CCT020312上调PERK、eIF2α磷酸化水平和ATF4、CHOP蛋白表达后,FABP4沉默对GDM大鼠IR、炎症、氧化应激以及胰腺和胎盘损伤的抑制作用均被逆转(P<0.05)。FABP4在HG诱导的HTR-8/SVneo细胞中上调表达,沉默FABP4可抑制PERK/eIF2α/ATF4/CHOP通路蛋白表达,CCT020312则逆转这种变化(P<0.05)。结论:FABP4沉默通过抑制炎症和氧化应激,改善GDM大鼠的IR和ERS,其机制与抑制PERK/eIF2α/ATF4/CHOP信号通路有关。 展开更多
关键词 FABP4 PERK/eIF2α/ATF4/CHOP信号通路 妊娠糖尿病 内质网应激 胰岛素抵抗
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基于内质网应激PERK/ATF4/CHOP信号通路探讨丹参多酚酸盐对膜性肾病大鼠足细胞凋亡的影响
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作者 郭明鑫 高圣柠 +4 位作者 吴怡欢 毛竞宇 唐淼 吴红红 檀金川 《江苏中医药》 CAS 2024年第9期67-73,共7页
目的:基于蛋白激酶R样内质网激酶(PERK)/活化转录因子4(ATF4)/转录因子C/EBP同源蛋白(CHOP)信号通路探讨丹参多酚酸盐对膜性肾病(MN)大鼠内质网应激的影响。方法:将80只雄性SD大鼠随机分配,其中20只为正常组,剩余60只大鼠采用尾静脉注... 目的:基于蛋白激酶R样内质网激酶(PERK)/活化转录因子4(ATF4)/转录因子C/EBP同源蛋白(CHOP)信号通路探讨丹参多酚酸盐对膜性肾病(MN)大鼠内质网应激的影响。方法:将80只雄性SD大鼠随机分配,其中20只为正常组,剩余60只大鼠采用尾静脉注射阳离子化牛血清白蛋白(C-BSA)的方法诱导MN病变。将造模成功的大鼠随机分为模型组、贝那普利组和丹参多酚酸盐低、中、高剂量组,每组10只,另取10只正常组大鼠共同进行实验。贝那普利组每日给药剂量为10 mg/kg,丹参多酚酸盐低、中、高剂量组每日给药剂量分别为16.7、33.3、66.7 mg/kg,正常组和模型组予等体积生理盐水,各组均每日灌胃1次,连续4周。检测各组大鼠治疗后24 h尿蛋白定量(24 h-UTP);腹主动脉取血分离血清,检测各组大鼠血尿素氮(BUN)、肌酐(Scr)含量;过碘酸六胺银(PASM)染色后光镜观察各组大鼠肾组织病理形态,透射电子显微镜进一步观察各组大鼠肾组织细微结构变化;免疫荧光法检测各组大鼠肾组织免疫球蛋白G(IgG)沉积情况;免疫组化(IHC)法检测各组大鼠肾组织足细胞裂隙膜蛋白(Podocin)、足细胞裂孔隔膜蛋白(Nephrin)表达水平;蛋白免疫印迹(Western blot)法检测各组大鼠肾组织PERK、ATF4、CHOP、B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)表达。结果:相较于正常组,模型组大鼠的肾组织显示出显著的病理性变化,肾小球基底膜显著增厚,并出现类似钉突的结构改变,同时伴有系膜基质的增生现象,沿毛细血管袢有IgG弥漫性沉积;各给药组大鼠肾组织上述病理改变有所缓解,足细胞损伤减少,IgG沉积减轻。模型组大鼠24 h-UTP水平显著高于正常组(P<0.01);各给药组大鼠24 h-UTP水平均显著低于模型组(P<0.05,P<0.01),其中丹参多酚酸盐中剂量组大鼠24 h-UTP水平与贝那普利组比较差异无统计学意义(P>0.05),丹参多酚酸盐高剂量组大鼠24 h-UTP水平明显低于贝那普利组(P<0.01),丹参多酚酸盐对MN模型大鼠24 h-UTP水平的降低作用具有明显的剂量依赖性(P<0.01)。各组大鼠血清BUN、Scr水平比较差异均无统计学意义(P>0.05)。模型组大鼠肾组织Podocin、Nephrin蛋白表达显著低于正常组(P<0.05);各给药组大鼠上述蛋白表达均显著高于模型组(P<0.05),其中丹参多酚酸盐中剂量组大鼠上述蛋白表达水平与贝那普利组比较差异无统计学意义(P>0.05),丹参多酚酸盐高剂量组大鼠上述蛋白表达水平明显高于贝那普利组(P<0.05),丹参多酚酸盐对MN模型大鼠上述蛋白表达水平的升高作用具有明显的剂量依赖性(P<0.05)。模型组大鼠肾组织PERK、ATF4、CHOP、Bax蛋白表达水平均明显高于正常组(P<0.01),Bcl-2蛋白表达水平明显低于正常组(P<0.01);各给药组大鼠肾组织上述蛋白表达水平均较模型组显著改善(P<0.05,P<0.01),其中丹参多酚酸盐中剂量组大鼠上述蛋白表达水平与贝那普利组比较差异无统计学意义(P>0.05),丹参多酚酸盐高剂量组大鼠上述蛋白表达的改善程度显著优于贝那普利组(P<0.01),丹参多酚酸盐对MN模型大鼠上述蛋白表达水平的改善作用具有明显的剂量依赖性(P<0.01)。结论:丹参多酚酸盐可能通过调控PERK/ATF4/CHOP信号通路,缓解肾组织内质网应激,抑制足细胞凋亡,进而保护肾脏及延缓疾病进展。 展开更多
关键词 膜性肾病 丹参多酚酸盐 内质网应激 PERK/ATF4/CHOP信号通路 凋亡 盐酸贝那普利
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降脂通脉饮对高血脂症大鼠血脂和PERK/ATF4/CHOP信号通路的影响 被引量:1
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作者 郭锦荣 黄微 +3 位作者 吕丹丹 颜靖文 周芬敏 陆瑞峰 《现代中西医结合杂志》 CAS 2024年第4期465-468,479,共5页
目的观察降脂通脉饮对高血脂症大鼠血脂及蛋白激酶样内质网激酶(PERK)/转录激活因子4(ATF4)/C/EBP同源蛋白(CHOP)信号通路的影响,探讨降脂通脉饮调节血脂的作用机制。方法将30只SD大鼠随机分为正常组、模型组及降脂通脉饮低、中、高剂量... 目的观察降脂通脉饮对高血脂症大鼠血脂及蛋白激酶样内质网激酶(PERK)/转录激活因子4(ATF4)/C/EBP同源蛋白(CHOP)信号通路的影响,探讨降脂通脉饮调节血脂的作用机制。方法将30只SD大鼠随机分为正常组、模型组及降脂通脉饮低、中、高剂量组,每组6只。正常组大鼠给予普通饲料喂养,同时灌胃生理盐水;其余组大鼠给予高脂饲料喂养,其中模型组同时灌胃生理盐水,降脂通脉饮低、中、高剂量组同时灌胃1.56 mg/(kg·d)、3.125 mg/(kg·d)、6.25 mg/(kg·d)的降脂通脉饮。连续干预8周后,生化法检测血清胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平,RT-PCR和Western blot法检测颈动脉组织中PERK、ATF4、CHOPmRNA及蛋白表达情况。结果与正常组比较,模型组大鼠血清TC、TG、LDL-C水平和PERK、ATF4、CHOP mRNA及蛋白相对表达量均明显升高(P均<0.05);与模型组比较,降脂通脉饮中、高剂量组大鼠血清TC、TG、LDL-C水平和PERK、ATF4、CHOP mRNA及蛋白相对表达量均明显降低(P均<0.05)。结论降脂通脉饮可能通过调节内质网应激PERK/ATF4/CHOP信号通路而发挥降脂作用。 展开更多
关键词 高血脂症 降脂通脉饮 PERK/ATF4/CHOP信号通路
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基于PERK/eIF2α/ATF4/CHOP通路探讨健骨颗粒对UMR-106细胞内质网应激凋亡的影响 被引量:1
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作者 陈赛楠 周芬 +1 位作者 黄云梅 林燕萍 《康复学报》 CSCD 2024年第1期34-43,共10页
目的探讨健骨颗粒含药血清对UMR-106成骨样细胞内质网应激ERS凋亡的影响和作用机制。方法选用UMR-106成骨样细胞,采用0、0.01、0.02、0.03、0.04、0.05μmol/L不同浓度的GSK2606414(PERK抑制剂)对细胞进行干预,采用CCK8法筛选GSK260641... 目的探讨健骨颗粒含药血清对UMR-106成骨样细胞内质网应激ERS凋亡的影响和作用机制。方法选用UMR-106成骨样细胞,采用0、0.01、0.02、0.03、0.04、0.05μmol/L不同浓度的GSK2606414(PERK抑制剂)对细胞进行干预,采用CCK8法筛选GSK2606414最佳干预浓度。将生长状态较好的UMR-106细胞随机分为阴性对照组(NC组)、模型组(H_(2)O_(2)组)、健骨颗粒组(H_(2)O_(2)+JG组)和阳性对照组(H_(2)O_(2)+GSK2606414组)4组。NC组和H_(2)O_(2)组采用10%生理盐水血清干预12 h,H_(2)O_(2)+JG组采用10%健骨颗粒含药血清干预12 h,H_(2)O_(2)+GSK2606414组采用0.03μmol/L的GSK2606414和10%生理盐水血清干预12 h,除NC组外,其余各组在不更换新培养基的情况下,再分别加入10μmol/L H_(2)O_(2)干预12 h。采用激光共聚焦显微镜观察细胞内NC组和H_(2)O_(2)组GRP78和Caspase-12荧光表达情况,DCFH-DA检测活性氧(ROS)含量,激光共聚焦显微镜观察细胞内钙离子实时动态变化判断ROS/ERS模型是否成立。再采用An⁃nexin V-FITC/PI检测4组细胞晚期凋亡率,qPCR和Western blot检测4组ERS相关标志指标GRP78、PERK、eIf2α、ATF4和CHOP mRNA转录水平和蛋白相对表达量。结果与0μmol/L组比较,0.03μmol/L的GSK2606414是干预UMR-106细胞12 h后对细胞活力没有影响的最大浓度,故使用该浓度作为后续H_(2)O_(2)+GSK2606414组的实验干预条件。与NC组相比,H_(2)O_(2)组ROS含量显著增高(P<0.01),GRP78和Caspase-12的蛋白荧光表达量明显增加,细胞内钙离子流动速度加快并持续增高,表明H_(2)O_(2)诱导UMR-106细胞ROS/ERS模型的成功建立。与NC组相比,H_(2)O_(2)组凋亡率显著增高(P<0.05),GRP78、PERK、eIf2α、ATF4、CHOP mRNA转录水平和蛋白相对表达量均显著增高(P<0.01)。与H_(2)O_(2)组相比,H_(2)O_(2)+JG组和H_(2)O_(2)+GSK2606414组ROS含量显著降低(P<0.01),凋亡率显著降低(P<0.05),GRP78、PERK、eIf2α、ATF4、CHOP mRNA转录水平(P<0.05)和蛋白相对表达量(P<0.01)均显著降低。结论健骨颗粒可通过PERK/eIF2α/ATF4/CHOP信号通路缓解内质网过度应激,降低成骨细胞凋亡率,发挥防治绝经后骨质疏松症的作用。 展开更多
关键词 绝经后骨质疏松症 内质网应激 健骨颗粒 PERK/eIF2α/ATF4/CHOP信号通路 成骨细胞凋亡
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Design and parameter optimization of spiral-dragon type straw chopping test rig 被引量:1
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作者 Pengbo Ma Liqiao Li +3 位作者 Baoqin Wen Yunheng Xue Za Kan Jingbin Li 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2020年第1期47-56,共10页
In order to address the problems of large chop length,high energy consumption and poor chopping effect that were associated with a horizontal total mixed rations(TMR)mixer,a straw chopping device was designed;further,... In order to address the problems of large chop length,high energy consumption and poor chopping effect that were associated with a horizontal total mixed rations(TMR)mixer,a straw chopping device was designed;further,its structure and working principle were analyzed.In this study,wheat straw was chosen as the test object.The number of processing times,number of fixed blades,cutting speed,number of tooth plates and the inclined angle of the box were considered as the test factors.The standard straw length rate and average power were used as evaluation indices for conducting the orthogonal rotation combination experiment with five factors and five levels of quadratic regression.The experimental results denoted that the order of influencing factors on the standard straw length rate can be given as follows:the number of processing times,number of fixed blades,cutting speed,number of tooth plates and inclined angle of the box.The order of the influence of each factor on the average power can be given as follows:the cutting speed,number of fixed blades and number of tooth plates.Further,the optimum structural and working parameters of the device can be determined as follows:linear cutting speed of 17-20 m/min,box inclined angle of 70°-80°,20-23 fixed blades,5-6 processing times and 4-7 tooth plates.Under such a circumstance,the standard straw length rate reached up to 70.5%-77.4%and the average power was low at 5.8-7 kW.The results can be used as reference for designing a chopping device for a TMR mixer. 展开更多
关键词 total mixed rations STRAW chopping device parameter optimization
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Ethanol extract of Abelmoschus manihot suppresses endoplasmic reticulum stress in contrast-induced nephropathy
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作者 Xin Lin Xin Lu +3 位作者 Yun-He Zhao Yi-Bei Wang Ru-Ge Niu Xiao-Hu Chen 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2024年第1期17-27,I0002,I0003,共13页
Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2... Objective:To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot(L.)Medic in contrast-induced nephropathy(CIN).Methods:CIN rat models and human renal proximal tubular cells(HK-2)with iopromide-induced injury were employed to mimic CIN conditions.The effect of Abelmoschus manihot extract on the rat models and HK-2 cells was evaluated.In rat models,kidney function,histology,oxidative stress and apoptosis were determined.In HK-2 cells,cell viability,apoptosis,mitochondrial membrane potential,and endoplasmic reticulum stress were assessed.Results:Abelmoschus manihot extract significantly improved structural and functional impairments in the kidneys of CIN rats.Additionally,the extract effectively mitigated the decline in cellular viability and reduced iopromide-induced oxidative stress and lipid peroxidation.Mechanistic investigations revealed that Abelmoschus manihot extract prominently attenuated acute endoplasmic reticulum stress-mediated apoptosis by downregulating GRP78 and CHOP protein levels.Conclusions:Abelmoschus manihot extract can be used as a promising therapeutic and preventive agent in the treatment of CIN. 展开更多
关键词 Contrast-induced nephropathy Abelmoschus manihot extract ER stress APOPTOSIS GRP78 CHOP
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