AIM: To evaluate the effect of antisense oligonucleotide targeting midkine (MK-AS) on angiogenesis in chick chorioallantoic membrane (CAM) and in situ human hepatocellular carcinoma (HCC). METHODS: An in situ human he...AIM: To evaluate the effect of antisense oligonucleotide targeting midkine (MK-AS) on angiogenesis in chick chorioallantoic membrane (CAM) and in situ human hepatocellular carcinoma (HCC). METHODS: An in situ human hepatocellular carcinoma (HCC) model and CAM assay were used in this experiment. The effect of MK-AS on angiogenesis was evaluated by cell proliferation assay and hematoxylin- eosin (HE) staining. RESULTS: MK-AS significantly inhibited human umbilical vein endothelial cells (HUVEC) and in situ human HCC growth. At the same time, MK-AS suppressed the angiogenesis both in human hepatocellular carcinoma cell line (HEPG2)-induced CAM and in situ human HCC tissues. CONCLUSION: MK-AS is an effective antiangiogenesis agent in vivo.展开更多
Objective:To determine the toxicity profile,anti-angiogenic and antibacterial activity of the crude and semi-crude leaf extracts of Tinomiscium philippinense(T.philippinense).Methods:The leaves of T.philippinense were...Objective:To determine the toxicity profile,anti-angiogenic and antibacterial activity of the crude and semi-crude leaf extracts of Tinomiscium philippinense(T.philippinense).Methods:The leaves of T.philippinense were extracted with methanol and partitioned with solvents of different polarities,namely,hexane,dichloromethane and butanol.The extracts were subjected to duck chorioallantoic membrane assay to establish its anti-angiogenic property.Microwell assay was utilized to determine the minimum inhibitory concentration and minimum bactericidal concentration of the different extracts of the plant.Results:The dichloromethane leaf extract of T.philippinense at 1000μg/disc showed the highest anti-angiogenic activity with 37.46%inhibition.All the fractions exhibited a bacteriostatic and bactericidal effect on the three bacterial strains with Pseudomonas aeruginosa,a Gram negative lactose fermenter exhibiting a higher sensitivity to dichloromethane semi-crude extract among the treatment groups.For the toxicity test,no mortality and no change in behavior were observed in the Sprague-Dawley rats 14 days after the oral administration of the plant extracts.The methanolic leaf extract of T.philippinense is non-toxic at a maximum dose of 5000 mg/kg.Conclusions:The dichloromethane leaf extract of T.philippinense is a potential antiangiogenic endemic plant species.This plant extract is also a potential antibacterial candidate as determined by microwell assay.The anti-angiogenic and antibacterial activity of the plant may be attributed to the essential oil,steroid,flavonoid,sterol and triterpene content of the plant.展开更多
基金grants from Medical and Sanitary Research Foundation of Zhejiang Province, (No. 2003A077)Huzhou Natural Science Foundation, (No. 2004SZX07-11)
文摘AIM: To evaluate the effect of antisense oligonucleotide targeting midkine (MK-AS) on angiogenesis in chick chorioallantoic membrane (CAM) and in situ human hepatocellular carcinoma (HCC). METHODS: An in situ human hepatocellular carcinoma (HCC) model and CAM assay were used in this experiment. The effect of MK-AS on angiogenesis was evaluated by cell proliferation assay and hematoxylin- eosin (HE) staining. RESULTS: MK-AS significantly inhibited human umbilical vein endothelial cells (HUVEC) and in situ human HCC growth. At the same time, MK-AS suppressed the angiogenesis both in human hepatocellular carcinoma cell line (HEPG2)-induced CAM and in situ human HCC tissues. CONCLUSION: MK-AS is an effective antiangiogenesis agent in vivo.
基金Supported by the Research Center for the Natural and Applied Sciences and the DOST-National Science Consortium.
文摘Objective:To determine the toxicity profile,anti-angiogenic and antibacterial activity of the crude and semi-crude leaf extracts of Tinomiscium philippinense(T.philippinense).Methods:The leaves of T.philippinense were extracted with methanol and partitioned with solvents of different polarities,namely,hexane,dichloromethane and butanol.The extracts were subjected to duck chorioallantoic membrane assay to establish its anti-angiogenic property.Microwell assay was utilized to determine the minimum inhibitory concentration and minimum bactericidal concentration of the different extracts of the plant.Results:The dichloromethane leaf extract of T.philippinense at 1000μg/disc showed the highest anti-angiogenic activity with 37.46%inhibition.All the fractions exhibited a bacteriostatic and bactericidal effect on the three bacterial strains with Pseudomonas aeruginosa,a Gram negative lactose fermenter exhibiting a higher sensitivity to dichloromethane semi-crude extract among the treatment groups.For the toxicity test,no mortality and no change in behavior were observed in the Sprague-Dawley rats 14 days after the oral administration of the plant extracts.The methanolic leaf extract of T.philippinense is non-toxic at a maximum dose of 5000 mg/kg.Conclusions:The dichloromethane leaf extract of T.philippinense is a potential antiangiogenic endemic plant species.This plant extract is also a potential antibacterial candidate as determined by microwell assay.The anti-angiogenic and antibacterial activity of the plant may be attributed to the essential oil,steroid,flavonoid,sterol and triterpene content of the plant.
