Background:The chronic unpredictable mild stress(CUMS)model has long been considered the best model for exploring the pathophysiological mechanisms underlying depression.However,there are no widely recognised standard...Background:The chronic unpredictable mild stress(CUMS)model has long been considered the best model for exploring the pathophysiological mechanisms underlying depression.However,there are no widely recognised standards for strategies for modeling and for behavioral testing.The present study aimed to optimize the protocols for food deprivation and the sucrose preference test(SPT)for the CUMS model.Methods:We first evaluated the effects of different long periods of food deprivation on the body weight of Sprague Dawley(SD)rats by testing food deprivation for 24 hours(8:00-8:00^+),food deprivation for 12 hours during the daytime(8:00-20:00)and food deprivation for 12 hours at night(20:00-8:00^+).Next,we established a SD rat CUMS model with 15 different stimulations,and used body weight measurement,SPT,forced swim test(FST),open field test(OFT)and Morris water maze(MWM)test to verify the success of the modeling.In the SPT,consumption of sucrose and pure water within 1 and 12 hours was measured.Results:Twelve hours of food deprivation during the daytime(8:00-20:00)had no effect on body weight,while 12 hours of food deprivation at night(20:00-8:00^+)and 24 hours of food deprivation(8:00-8:00^+)significantly reduced the mean body weight of the SD rats.When SPT was used to verify the successful establishment of the CUMS rat model,sucrose consumption measured within 12 hours was less variable than that measured within 1 hour.Conclusions:Twelve hours of food deprivation in the daytime(8:00-20:00)may be considered a mild stimulus for the establishment of a CUMS rat model.Measuring sucrose consumption over 12 hours is recommended for SPT.展开更多
[Objectives]To explore the antidepressant effect of Shenwei Ningyu Tablet,a new antidepressant traditional Chinese medicine,on rat chronic stress depression model and mouse tail suspension models.[Methods]Rat chronic ...[Objectives]To explore the antidepressant effect of Shenwei Ningyu Tablet,a new antidepressant traditional Chinese medicine,on rat chronic stress depression model and mouse tail suspension models.[Methods]Rat chronic stress model:except for the normal group,the rats in other groups were given corresponding chronic stress,and administered by gavage 1 h before modeling,for a total of 21 d.The changes of each indicator before and after the experiment were observed through the body weight change,the sugar water test,and open field test.The relevant hormone levels were detected by radioimmunoassay.Mouse tail suspension depression model:after continuous administration for 7 d,the activity times was recorded with the mouse automatic recorder,and the mouse immobility time was recorded after tail suspension,to explore the effects of each administration group on the tail suspension immobility time of mice.[Results]Chronic stress depression model:21 d after modeling,compared with the normal group,rats in the model group exhibited significantly reduced body weight,sucrose preference index,and horizontal and vertical movement scores(P<0.05).Compared with the model group,the low-dose Shenwei Ningyu Tablets group had significant differences in the sugar water test,horizontal and vertical movement scores(P<0.05).In addition,all three dose groups of Shenwei Ningyu Tablets could effectively reduce the content of CRF in chronic stress model rats,and the low dose group could significantly reduce the ACTH level in model rats(P<0.05).Mouse tail suspension depression model:the immobility time after tail suspension in each administration group was significantly different from that in the model group(P<0.05).[Conclusions]Shenwei Ningyu Tablets has a certain anti-depression effect on both the rat chronic stress depression model and the mouse tail suspension depression model.展开更多
目的:研究不同时程低强度束缚水浸应激对大鼠Cajal间质细胞(interstitial cells of Cajal,ICC)数量的影响,探讨慢性应激导致胃动力改变与ICC的相关性.方法:雄性SPF级SD大鼠48只被随机分为6组,即实验3d、7d、28d组和对照3d、7d、28d组,每...目的:研究不同时程低强度束缚水浸应激对大鼠Cajal间质细胞(interstitial cells of Cajal,ICC)数量的影响,探讨慢性应激导致胃动力改变与ICC的相关性.方法:雄性SPF级SD大鼠48只被随机分为6组,即实验3d、7d、28d组和对照3d、7d、28d组,每组8只.实验组23℃水域箱内束缚水浸1h/d,对照组自由摄食饮水.4d、8d、29d晨序贯脱颈处死.取膜胃小弯侧(ICC-1)、腺胃小弯侧(ICC-3)、腺胃大弯侧上1/3处(ICC-5)、腺胃幽门旁0.5cm处(ICC-7)组织各一块放入固定液中,制成石蜡切片;抗c-kit免疫组织化学染色,观察不同层次ICC计数.结果:正常大鼠胃内ICC主要分布在肌内(ICC-MY)和肌间(ICC-IM),而黏膜下(ICC-SM)和深肌丛(ICC-DMP)较少.实验3d组的各层ICC数量与对照组相比无明显差异,实验7d和28d组大鼠不同部位、不同层次及同一部位不同层次ICC数量明显异于同期对照组.应激时间长短对不同部位不同层次及同一部位不同层次ICC总数有明显影响,其大致规律是先正常后增加再减少,即先升高后降低.应激主要引起ICC-MY及ICC-IM数量发生变化,而本来较少的ICC-SM和ICC-DMP受影响较轻.结论:长时间低强度的慢性束缚水浸应激可以使大鼠胃窦ICC数量发生变化.展开更多
基金This work was supported by Social livelihood projects of Chongqing Science and Technology Bureau(cstc2017shms-zdyfX0048,csts2017shmsA00007).
文摘Background:The chronic unpredictable mild stress(CUMS)model has long been considered the best model for exploring the pathophysiological mechanisms underlying depression.However,there are no widely recognised standards for strategies for modeling and for behavioral testing.The present study aimed to optimize the protocols for food deprivation and the sucrose preference test(SPT)for the CUMS model.Methods:We first evaluated the effects of different long periods of food deprivation on the body weight of Sprague Dawley(SD)rats by testing food deprivation for 24 hours(8:00-8:00^+),food deprivation for 12 hours during the daytime(8:00-20:00)and food deprivation for 12 hours at night(20:00-8:00^+).Next,we established a SD rat CUMS model with 15 different stimulations,and used body weight measurement,SPT,forced swim test(FST),open field test(OFT)and Morris water maze(MWM)test to verify the success of the modeling.In the SPT,consumption of sucrose and pure water within 1 and 12 hours was measured.Results:Twelve hours of food deprivation during the daytime(8:00-20:00)had no effect on body weight,while 12 hours of food deprivation at night(20:00-8:00^+)and 24 hours of food deprivation(8:00-8:00^+)significantly reduced the mean body weight of the SD rats.When SPT was used to verify the successful establishment of the CUMS rat model,sucrose consumption measured within 12 hours was less variable than that measured within 1 hour.Conclusions:Twelve hours of food deprivation in the daytime(8:00-20:00)may be considered a mild stimulus for the establishment of a CUMS rat model.Measuring sucrose consumption over 12 hours is recommended for SPT.
基金National Major Scientific and Technological Special Project for"Significant New Drugs Development"(2019ZX09301-005).
文摘[Objectives]To explore the antidepressant effect of Shenwei Ningyu Tablet,a new antidepressant traditional Chinese medicine,on rat chronic stress depression model and mouse tail suspension models.[Methods]Rat chronic stress model:except for the normal group,the rats in other groups were given corresponding chronic stress,and administered by gavage 1 h before modeling,for a total of 21 d.The changes of each indicator before and after the experiment were observed through the body weight change,the sugar water test,and open field test.The relevant hormone levels were detected by radioimmunoassay.Mouse tail suspension depression model:after continuous administration for 7 d,the activity times was recorded with the mouse automatic recorder,and the mouse immobility time was recorded after tail suspension,to explore the effects of each administration group on the tail suspension immobility time of mice.[Results]Chronic stress depression model:21 d after modeling,compared with the normal group,rats in the model group exhibited significantly reduced body weight,sucrose preference index,and horizontal and vertical movement scores(P<0.05).Compared with the model group,the low-dose Shenwei Ningyu Tablets group had significant differences in the sugar water test,horizontal and vertical movement scores(P<0.05).In addition,all three dose groups of Shenwei Ningyu Tablets could effectively reduce the content of CRF in chronic stress model rats,and the low dose group could significantly reduce the ACTH level in model rats(P<0.05).Mouse tail suspension depression model:the immobility time after tail suspension in each administration group was significantly different from that in the model group(P<0.05).[Conclusions]Shenwei Ningyu Tablets has a certain anti-depression effect on both the rat chronic stress depression model and the mouse tail suspension depression model.
文摘目的:研究不同时程低强度束缚水浸应激对大鼠Cajal间质细胞(interstitial cells of Cajal,ICC)数量的影响,探讨慢性应激导致胃动力改变与ICC的相关性.方法:雄性SPF级SD大鼠48只被随机分为6组,即实验3d、7d、28d组和对照3d、7d、28d组,每组8只.实验组23℃水域箱内束缚水浸1h/d,对照组自由摄食饮水.4d、8d、29d晨序贯脱颈处死.取膜胃小弯侧(ICC-1)、腺胃小弯侧(ICC-3)、腺胃大弯侧上1/3处(ICC-5)、腺胃幽门旁0.5cm处(ICC-7)组织各一块放入固定液中,制成石蜡切片;抗c-kit免疫组织化学染色,观察不同层次ICC计数.结果:正常大鼠胃内ICC主要分布在肌内(ICC-MY)和肌间(ICC-IM),而黏膜下(ICC-SM)和深肌丛(ICC-DMP)较少.实验3d组的各层ICC数量与对照组相比无明显差异,实验7d和28d组大鼠不同部位、不同层次及同一部位不同层次ICC数量明显异于同期对照组.应激时间长短对不同部位不同层次及同一部位不同层次ICC总数有明显影响,其大致规律是先正常后增加再减少,即先升高后降低.应激主要引起ICC-MY及ICC-IM数量发生变化,而本来较少的ICC-SM和ICC-DMP受影响较轻.结论:长时间低强度的慢性束缚水浸应激可以使大鼠胃窦ICC数量发生变化.
