In many areas of China Cirsium setosum is used as Cirsiumjaponicum DC. Although the two herbs have similar appearance and many similar compounds, they are totally different medicinal material, and have different pharm...In many areas of China Cirsium setosum is used as Cirsiumjaponicum DC. Although the two herbs have similar appearance and many similar compounds, they are totally different medicinal material, and have different pharmacodynamic actions. The fingerprint spectrum can be a good tool to distinguish the two herbs and control the quality of Cirsium japonicum DC. In this paper, the chemical fingerprint of Cirsiumjaponicum DC was established using raw materials from 15 origins in China. The chromatographic separations were obtained by a SHIM-PACK VP-ODS column （150 mm ~ 4.6 mm i.d., 5 ~tm） using gradient elution, and run time of 80 min. The peak of linarin was considered as the control peak. The experimental data were analyzed with the software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （Version 2004A） and the quality control system of both the overall qualitative similari- ties and the overall quantitative similarities of traditional Chinese medicine chromatographic fingerprints.展开更多
Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water ex...Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied. Results EST electrophoresis showed that EST was an important antidotal enzyme system and reacted strongly to environment EST changed greatly during the whole exposure period so that it could be viewed as a pathological index of toxicity. Extracts decreased the glycogen content of the snails＇ soft tissues greatly, and also the protein content. ConcIusion All extracts show strong molluscicidal activity. The LD50 value of the water extract of N. indicum Mill is as low as 13.2 mg/L. EST can be viewed as a pathological index of toxicity. The energy metabolism abnormity is the key reason fnr the molluscicidal activities. The biochemical mechanism needs further research.展开更多
Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects...Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects of Daji （Cirsiumjaponicum） polysaccharide extracts （CJP） against hydrogen peroxide （H2O2） shock in rat H9c2 myocardial cells. First,CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquidchromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cellinjury model. The 3- （4,5- dimethylthiazol- 2-yl）-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatmentsignificantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. Inaddition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP onmyocardial cells. Therefore, we conclude that polysaccharide extracts of Daji （Cirsium japonicum） protect rat H9c2myocardial cells from oxidative stress induced by H2O2.展开更多
基金supported by the Main Program of Autonomous Region College Scientific Research Program (XJEDU2008145)
文摘In many areas of China Cirsium setosum is used as Cirsiumjaponicum DC. Although the two herbs have similar appearance and many similar compounds, they are totally different medicinal material, and have different pharmacodynamic actions. The fingerprint spectrum can be a good tool to distinguish the two herbs and control the quality of Cirsium japonicum DC. In this paper, the chemical fingerprint of Cirsiumjaponicum DC was established using raw materials from 15 origins in China. The chromatographic separations were obtained by a SHIM-PACK VP-ODS column （150 mm ~ 4.6 mm i.d., 5 ~tm） using gradient elution, and run time of 80 min. The peak of linarin was considered as the control peak. The experimental data were analyzed with the software of Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （Version 2004A） and the quality control system of both the overall qualitative similari- ties and the overall quantitative similarities of traditional Chinese medicine chromatographic fingerprints.
基金This work was supported by the National Natural Science Foundation of China (No. 30471506).
文摘Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied. Results EST electrophoresis showed that EST was an important antidotal enzyme system and reacted strongly to environment EST changed greatly during the whole exposure period so that it could be viewed as a pathological index of toxicity. Extracts decreased the glycogen content of the snails＇ soft tissues greatly, and also the protein content. ConcIusion All extracts show strong molluscicidal activity. The LD50 value of the water extract of N. indicum Mill is as low as 13.2 mg/L. EST can be viewed as a pathological index of toxicity. The energy metabolism abnormity is the key reason fnr the molluscicidal activities. The biochemical mechanism needs further research.
文摘Daji （Cirsium japonicum） has been applied against gastric disorders, lung diseases, and cardiovascular problems in thetraditional Chinese medicinal system. The present study was to investigate the protective effects of Daji （Cirsiumjaponicum） polysaccharide extracts （CJP） against hydrogen peroxide （H2O2） shock in rat H9c2 myocardial cells. First,CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquidchromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cellinjury model. The 3- （4,5- dimethylthiazol- 2-yl）-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatmentsignificantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. Inaddition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP onmyocardial cells. Therefore, we conclude that polysaccharide extracts of Daji （Cirsium japonicum） protect rat H9c2myocardial cells from oxidative stress induced by H2O2.
