Objective:To explore effect of Pemetrexed combined with cis-platinum chemotherapy on matrix metalloproteinase (MMPs), vascular esandothelial growth factor (VEGF), NK cells and immune function in patients with non-squa...Objective:To explore effect of Pemetrexed combined with cis-platinum chemotherapy on matrix metalloproteinase (MMPs), vascular esandothelial growth factor (VEGF), NK cells and immune function in patients with non-squamous non-small cell lung cancer.Method:A total of86 cases of non-squamous non-small cell lung cancer patients were divided into control group (n=44) and observation group (n=42), control group was given docetaxel combined cis-platinum chemotherapy, pemetrexed combined cis-platinum chemotherapy, was applied for observation group. Compared MMP-2, MMP-9, VEGF, NK cells and immune function level before and after treatment in both groups.Results: MMP-2, MMP-9, VEGF, NK cells, CD3+, CD4+, CD8+, CD4+/CD8+ level in both groups before treatment was no significant difference. After treatment, MMP-2, MMP-9, VEGF, CD8+level in both groups was significant lower than before treatment intra-group, and observation was lower than control group, there was significant difference. After treatment, NK cells, CD3+, CD4+, CD8+, CD4+/CD8+ level in both groups was increased dramatically than before treatment of intra-group, moreover, NK cells, CD3+, CD4+, CD8+, CD4+/CD8+level in observation group after treatment was obvious higher than in control group after treatment, there was significant difference.Conclusion:Pemetrexed combined with cis-platinum chemotherapy for non-squamous non-small cell lung cancer could effectively decrease serum MMPs, VEGF level and increase NK cell level, regulate immune function, with definite clinical significance.展开更多
Copolymer of divinyl ether and maleic anhydride (DVE-co-MA) derivatives of cis-platinum complexes were synthesized and characterized by elementary analysis, IR and XPS ( X-ray photoelectron spectroscopy). The behavior...Copolymer of divinyl ether and maleic anhydride (DVE-co-MA) derivatives of cis-platinum complexes were synthesized and characterized by elementary analysis, IR and XPS ( X-ray photoelectron spectroscopy). The behavior of the products in biological environment was also studied. UV-visible and fluorescence spectra show that these polymer derivatives are able to exchange ligands with selected nucleophilic groups in biological environment.展开更多
Objective: To discuss the the effect of FGFR4 gene silencing on growth, metastasis and the cis-platinum (DDP) chemotherapy sensitivity of Hela cervical cancer cells. Methods: The Hela cervical cancer cells were cultur...Objective: To discuss the the effect of FGFR4 gene silencing on growth, metastasis and the cis-platinum (DDP) chemotherapy sensitivity of Hela cervical cancer cells. Methods: The Hela cervical cancer cells were cultured and divided into 5 groups: blank control group, negative control group, FGFR4-siRNA group, DDP group and FGFR4-siRNA+DDP group. The siRNA targeted to FGFR4 was constructed and transfected into the cells in FGFR4-siRNA group and FGFR4-siRNA+DDP group, and DDP (10 μg/mL) was added into the DDP group and FGFR4-siRNA+DDP group. The growth inhibition ratio was detected by MTT and the apoptosis rate was detected by FCM after 24 h, 48 h and 72 h culturing respectively. The mRNA and protein expression level of FGFR4, VEGF and MMP2 were detected by RT-PCR and western blot respectively. Results: Compared with the blank control, the proliferative activity of FGFR4-siRNA group decreased after the FGFR4 being silenced, and the growth inhibition ratio and apoptosis rate increased, the FGFR4 did almost not express and the expression level of VEGF and MMP2 decreased.The growth inhibition ratio and apoptosis rate of FGFR4-siRNA+DDP group were higher than that of FGFR4-siRNA group and DDP group, the FGFR4 did almost not express either and the the expression level of VEGF and MMP2 were lower than the FGFR4-siRNA group and DDP group. Conclusion: It can be obtained that silencing the FGFR4 of cervical cancer could inhibit cell growth, promote cell apoptosis and increase the chemosensitivity, down-regulate the expression of VEGF and MMP2 to inhibit the ability of invasion and metastasis, which could be one of the targeted therapeutic targets of cervical cancer.展开更多
Objective:To investigate the effect of Delisheng Injection（得力生注射液 DLS）,a Chinese medicinal compound,DLS combined with cis-platinum（DDP）,an active agent used in lung cancer chemotherapy,on a human highly meta...Objective:To investigate the effect of Delisheng Injection（得力生注射液 DLS）,a Chinese medicinal compound,DLS combined with cis-platinum（DDP）,an active agent used in lung cancer chemotherapy,on a human highly metastatic giant lung carcinoma cell line PGCL3.Methods:The suspended PGCL3 cells at10;/mL cultured in 96-well tissue culture plates were divided into 4 groups:DLS treatment group（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）,DDP treatment group（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL）,combined DLS with DDP treatment group（DLS:DDP 2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL）and a control group.The cytotoxicity of DLS with different concentrations（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）on PGCL3 cells was determined by 3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide（MTT） assay.Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay.Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.Results:Compared with the control group,DLS（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL） could significantly decrease cell proliferation,adhesion,invasion and migration abilities（P<0.05）.Cell adhesion,invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP（2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL） compared with DDP single-agent treatment（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL,P<0.05）,respectively.Conclusions:DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis.Our research provided a experimental basis about the treatment on highly metastatic lung caner.展开更多
Background A phase Ⅲ trial involving docetaxel, cisplatin, and fluorouracil （DCF） in the treatment of advanced gastric cancer was shown to have superior efficacy compared to cisplatin and fluorouracil alone, but wi...Background A phase Ⅲ trial involving docetaxel, cisplatin, and fluorouracil （DCF） in the treatment of advanced gastric cancer was shown to have superior efficacy compared to cisplatin and fluorouracil alone, but with a high rate of hematologic toxicity. To reduce toxicity while maintaining the efficacy of DCF, we reduced the doses of docetaxel （D） and cis-platinum （CDDP）, and administered 5-fluorouracil （5-FU） via a continuous intravenous （CIV） infusion.Methods Chemotherapy-naive patients with gastric adenocarcinomas received D （60 mg/m2 1 hour on day 1）, CDDP （30 mg/m2on days 1 and 2）, and 5-FU （1500 mg·m-2·24 h-1 CIV on days 1 and 8 every 3 weeks）. The primary endpoint was the response rate.Results Fourteen patients were enrolled. Based on the efficacy evaluation following at least 2 cycles of treatment, there was 7.1% complete remission （CR）, 71% partial remission （PR）, 14% stable disease （NC/SD）, and 7.1% progressive disease （PD）. The median survival time was 13 months. Nine patients （64%） had grade Ⅲ-Ⅳ neutropenia, and 4 patients （29%） had grade Ⅳ neutropenia, among whom 1 had grade Ⅳ neutropenia with grade Ⅲ nausea and vomiting.Conclusion The modified DCF regimen is highly active and has a favorable toxicity profile in Chinese patients with gastric cancer.展开更多
文摘Objective:To explore effect of Pemetrexed combined with cis-platinum chemotherapy on matrix metalloproteinase (MMPs), vascular esandothelial growth factor (VEGF), NK cells and immune function in patients with non-squamous non-small cell lung cancer.Method:A total of86 cases of non-squamous non-small cell lung cancer patients were divided into control group (n=44) and observation group (n=42), control group was given docetaxel combined cis-platinum chemotherapy, pemetrexed combined cis-platinum chemotherapy, was applied for observation group. Compared MMP-2, MMP-9, VEGF, NK cells and immune function level before and after treatment in both groups.Results: MMP-2, MMP-9, VEGF, NK cells, CD3+, CD4+, CD8+, CD4+/CD8+ level in both groups before treatment was no significant difference. After treatment, MMP-2, MMP-9, VEGF, CD8+level in both groups was significant lower than before treatment intra-group, and observation was lower than control group, there was significant difference. After treatment, NK cells, CD3+, CD4+, CD8+, CD4+/CD8+ level in both groups was increased dramatically than before treatment of intra-group, moreover, NK cells, CD3+, CD4+, CD8+, CD4+/CD8+level in observation group after treatment was obvious higher than in control group after treatment, there was significant difference.Conclusion:Pemetrexed combined with cis-platinum chemotherapy for non-squamous non-small cell lung cancer could effectively decrease serum MMPs, VEGF level and increase NK cell level, regulate immune function, with definite clinical significance.
基金Project supported by the Science Fund of the Chinese Academy of Sciences.
文摘Copolymer of divinyl ether and maleic anhydride (DVE-co-MA) derivatives of cis-platinum complexes were synthesized and characterized by elementary analysis, IR and XPS ( X-ray photoelectron spectroscopy). The behavior of the products in biological environment was also studied. UV-visible and fluorescence spectra show that these polymer derivatives are able to exchange ligands with selected nucleophilic groups in biological environment.
文摘Objective: To discuss the the effect of FGFR4 gene silencing on growth, metastasis and the cis-platinum (DDP) chemotherapy sensitivity of Hela cervical cancer cells. Methods: The Hela cervical cancer cells were cultured and divided into 5 groups: blank control group, negative control group, FGFR4-siRNA group, DDP group and FGFR4-siRNA+DDP group. The siRNA targeted to FGFR4 was constructed and transfected into the cells in FGFR4-siRNA group and FGFR4-siRNA+DDP group, and DDP (10 μg/mL) was added into the DDP group and FGFR4-siRNA+DDP group. The growth inhibition ratio was detected by MTT and the apoptosis rate was detected by FCM after 24 h, 48 h and 72 h culturing respectively. The mRNA and protein expression level of FGFR4, VEGF and MMP2 were detected by RT-PCR and western blot respectively. Results: Compared with the blank control, the proliferative activity of FGFR4-siRNA group decreased after the FGFR4 being silenced, and the growth inhibition ratio and apoptosis rate increased, the FGFR4 did almost not express and the expression level of VEGF and MMP2 decreased.The growth inhibition ratio and apoptosis rate of FGFR4-siRNA+DDP group were higher than that of FGFR4-siRNA group and DDP group, the FGFR4 did almost not express either and the the expression level of VEGF and MMP2 were lower than the FGFR4-siRNA group and DDP group. Conclusion: It can be obtained that silencing the FGFR4 of cervical cancer could inhibit cell growth, promote cell apoptosis and increase the chemosensitivity, down-regulate the expression of VEGF and MMP2 to inhibit the ability of invasion and metastasis, which could be one of the targeted therapeutic targets of cervical cancer.
基金Supported by the Project of Science and Technology Development in Shaanxi Province of China(No.2008KG10-01)
文摘Objective:To investigate the effect of Delisheng Injection（得力生注射液 DLS）,a Chinese medicinal compound,DLS combined with cis-platinum（DDP）,an active agent used in lung cancer chemotherapy,on a human highly metastatic giant lung carcinoma cell line PGCL3.Methods:The suspended PGCL3 cells at10;/mL cultured in 96-well tissue culture plates were divided into 4 groups:DLS treatment group（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）,DDP treatment group（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL）,combined DLS with DDP treatment group（DLS:DDP 2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL）and a control group.The cytotoxicity of DLS with different concentrations（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）on PGCL3 cells was determined by 3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide（MTT） assay.Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay.Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.Results:Compared with the control group,DLS（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL） could significantly decrease cell proliferation,adhesion,invasion and migration abilities（P<0.05）.Cell adhesion,invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP（2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL） compared with DDP single-agent treatment（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL,P<0.05）,respectively.Conclusions:DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis.Our research provided a experimental basis about the treatment on highly metastatic lung caner.
文摘Background A phase Ⅲ trial involving docetaxel, cisplatin, and fluorouracil （DCF） in the treatment of advanced gastric cancer was shown to have superior efficacy compared to cisplatin and fluorouracil alone, but with a high rate of hematologic toxicity. To reduce toxicity while maintaining the efficacy of DCF, we reduced the doses of docetaxel （D） and cis-platinum （CDDP）, and administered 5-fluorouracil （5-FU） via a continuous intravenous （CIV） infusion.Methods Chemotherapy-naive patients with gastric adenocarcinomas received D （60 mg/m2 1 hour on day 1）, CDDP （30 mg/m2on days 1 and 2）, and 5-FU （1500 mg·m-2·24 h-1 CIV on days 1 and 8 every 3 weeks）. The primary endpoint was the response rate.Results Fourteen patients were enrolled. Based on the efficacy evaluation following at least 2 cycles of treatment, there was 7.1% complete remission （CR）, 71% partial remission （PR）, 14% stable disease （NC/SD）, and 7.1% progressive disease （PD）. The median survival time was 13 months. Nine patients （64%） had grade Ⅲ-Ⅳ neutropenia, and 4 patients （29%） had grade Ⅳ neutropenia, among whom 1 had grade Ⅳ neutropenia with grade Ⅲ nausea and vomiting.Conclusion The modified DCF regimen is highly active and has a favorable toxicity profile in Chinese patients with gastric cancer.
