Objective:To investigate the diuretic and renal effects of Silybum marianum L.and Cistus ladaniferus L.in normal rats.Methods:Four groups of rats were used in each experiment.The first group received water,the second ...Objective:To investigate the diuretic and renal effects of Silybum marianum L.and Cistus ladaniferus L.in normal rats.Methods:Four groups of rats were used in each experiment.The first group received water,the second group received Cistus ladaniferus L.extract(100 mg/kg b.wt),the third group received Silybum marianum L.extract(100 mg/kg b.wt),and the fourth group received furosemide(10 mg/kg b.wt).Variables including urine volume,plasma and urine sodium,potassium and creatinine,and creatinine clearance were measured.Two experiments were conducted.A single dose of each intervention was used and the variables were measured during 24 h,and the interventions were given daily for a total of 8 d and the variables were measured during various intervals.Results:The single dose of each plant extract increased urine volume at all-time intervals and increased urine sodium and potassium excretion without affecting plasma sodium and potassium(P<0.05).On the day 8 after daily administration,the plant extracts induced a significant diuresis and natriuresis without affecting serum electrolytes(P<0.05),while furosemide caused hypokalemia.Both plant extracts significantly increased creatinine clearance(P<0.05).Conclusions:Silybum marianum L.and Cistus ladaniferus L Increase creatinine clearance and have a significant diuretic effect without affecting serum electrolytes.Silybum marianum L.is more potent than furosemide or Cistus ladaniferus L.展开更多
Objective:To evaluate the effect of aqueous leaf extract of Cistus ladaniferus(C.ladaniferus) on glycemic and lipidemic status in diabetic rats.Methods:Overnight fasted rats were injected intraperitoneally with strept...Objective:To evaluate the effect of aqueous leaf extract of Cistus ladaniferus(C.ladaniferus) on glycemic and lipidemic status in diabetic rats.Methods:Overnight fasted rats were injected intraperitoneally with streptozotocin(45 mg/kg) to induce experimental diabetes.The aqueous extract of the leaves of C.ladaniferus was administered orally at the dose of 500 mg/kg body weight to diabetic rats for a period of 28 days.Hypoglycemic effect,body weight,oral glucose tolerance,change in lipid parameters,urea,creatinine,aspartate aminotransferase and alanine aminotransferase levels of diabetic rats treated with aqueous extract were evaluated in experimental animals.Results:Administration of 500 mg/kg of C.ladaniferus extract to diabetic rats for 28 days resulted in a significant reduction in the levels of blood glucose,alanine aminotransferase,aspartate aminotransferase,urea and creatinine.Furthermore,the extract of C.ladaniferus improved glucose tolerance in diabetic rats,and its antidiabetic effect was similar to the one obtained with glibenclamide.The hypolipidemic effect was demonstrated by an important decrease in plasma total cholesterol,triglycerides and low density lipoprotein-cholesterol levels.Conclusions:It is concluded that C.ladaniferus leaf extract showed an antidiabetic activity in experimental diabetes which was similar to the one obtained with glibenclamide.展开更多
The purpose of this study was to investigate the primary mode of action of Cistus ladaniferus essential oil active fractions on Staphylococcus aureus strain ATCC6538P (CIP 53.156). The mode of inhibition of the active...The purpose of this study was to investigate the primary mode of action of Cistus ladaniferus essential oil active fractions on Staphylococcus aureus strain ATCC6538P (CIP 53.156). The mode of inhibition of the active fractions was assessed by determining the minimum inhibitory concentration (MIC). The effects of time on cell integrity were determined by time-kill, bacteriolysis and loss of 260 and 280-nm-absorbing material assays. Measurement of intra- and extracellular ATP was used to evaluate the energy remaining in the cells after treatment. A bacteriostatic and a bactericidal mode of inhibition were established respectively for acetate and alcohol fractions at their MIC. No intracellular material leakage and no lysis occurred after treatments with these fractions. In both cases, we observed a decrease of the ATP level within S. aureus cells whilst there was no proportional increase outside the cells. However, the effects induced by alcohols are more pronounced than those provoked by acetates. Indeed, marked structural changes were observed by transmission electron microscopy (TEM). The septal material of cells undergoing division became thicker and stained more lightly. The proportion of septa is also markedly increased and defective with respect to placement. These observations suggest a blocking in cell division, probably caused by the inhibition of ATPase or a disturbance in proton motrice force by the hydrophobic molecules viridiflorol and ledol, mainly present in alcohol fraction.展开更多
文摘Objective:To investigate the diuretic and renal effects of Silybum marianum L.and Cistus ladaniferus L.in normal rats.Methods:Four groups of rats were used in each experiment.The first group received water,the second group received Cistus ladaniferus L.extract(100 mg/kg b.wt),the third group received Silybum marianum L.extract(100 mg/kg b.wt),and the fourth group received furosemide(10 mg/kg b.wt).Variables including urine volume,plasma and urine sodium,potassium and creatinine,and creatinine clearance were measured.Two experiments were conducted.A single dose of each intervention was used and the variables were measured during 24 h,and the interventions were given daily for a total of 8 d and the variables were measured during various intervals.Results:The single dose of each plant extract increased urine volume at all-time intervals and increased urine sodium and potassium excretion without affecting plasma sodium and potassium(P<0.05).On the day 8 after daily administration,the plant extracts induced a significant diuresis and natriuresis without affecting serum electrolytes(P<0.05),while furosemide caused hypokalemia.Both plant extracts significantly increased creatinine clearance(P<0.05).Conclusions:Silybum marianum L.and Cistus ladaniferus L Increase creatinine clearance and have a significant diuretic effect without affecting serum electrolytes.Silybum marianum L.is more potent than furosemide or Cistus ladaniferus L.
基金Supported by University of Sidi Mohamed Ben Abdellah(USMBA)grant from the National Center for Scientific and Technical Research of Morocco(CNRST)to Mohamed El Kabbaoui(Fellowship number N°:021USMBA2014)
文摘Objective:To evaluate the effect of aqueous leaf extract of Cistus ladaniferus(C.ladaniferus) on glycemic and lipidemic status in diabetic rats.Methods:Overnight fasted rats were injected intraperitoneally with streptozotocin(45 mg/kg) to induce experimental diabetes.The aqueous extract of the leaves of C.ladaniferus was administered orally at the dose of 500 mg/kg body weight to diabetic rats for a period of 28 days.Hypoglycemic effect,body weight,oral glucose tolerance,change in lipid parameters,urea,creatinine,aspartate aminotransferase and alanine aminotransferase levels of diabetic rats treated with aqueous extract were evaluated in experimental animals.Results:Administration of 500 mg/kg of C.ladaniferus extract to diabetic rats for 28 days resulted in a significant reduction in the levels of blood glucose,alanine aminotransferase,aspartate aminotransferase,urea and creatinine.Furthermore,the extract of C.ladaniferus improved glucose tolerance in diabetic rats,and its antidiabetic effect was similar to the one obtained with glibenclamide.The hypolipidemic effect was demonstrated by an important decrease in plasma total cholesterol,triglycerides and low density lipoprotein-cholesterol levels.Conclusions:It is concluded that C.ladaniferus leaf extract showed an antidiabetic activity in experimental diabetes which was similar to the one obtained with glibenclamide.
文摘The purpose of this study was to investigate the primary mode of action of Cistus ladaniferus essential oil active fractions on Staphylococcus aureus strain ATCC6538P (CIP 53.156). The mode of inhibition of the active fractions was assessed by determining the minimum inhibitory concentration (MIC). The effects of time on cell integrity were determined by time-kill, bacteriolysis and loss of 260 and 280-nm-absorbing material assays. Measurement of intra- and extracellular ATP was used to evaluate the energy remaining in the cells after treatment. A bacteriostatic and a bactericidal mode of inhibition were established respectively for acetate and alcohol fractions at their MIC. No intracellular material leakage and no lysis occurred after treatments with these fractions. In both cases, we observed a decrease of the ATP level within S. aureus cells whilst there was no proportional increase outside the cells. However, the effects induced by alcohols are more pronounced than those provoked by acetates. Indeed, marked structural changes were observed by transmission electron microscopy (TEM). The septal material of cells undergoing division became thicker and stained more lightly. The proportion of septa is also markedly increased and defective with respect to placement. These observations suggest a blocking in cell division, probably caused by the inhibition of ATPase or a disturbance in proton motrice force by the hydrophobic molecules viridiflorol and ledol, mainly present in alcohol fraction.
