Objective:To investigate the correlation of cingulin and claudin-2 expression in ulcerative colitis (UC) tissues with intestinal flora disturbance, oxidative stress and inflammation. Methods: 70 patients with UC who w...Objective:To investigate the correlation of cingulin and claudin-2 expression in ulcerative colitis (UC) tissues with intestinal flora disturbance, oxidative stress and inflammation. Methods: 70 patients with UC who were diagnosed in our hospitals between July 2013 and February 2018 were selected as the UC group, and 90 patients with colonic polyps who underwent colonoscopy in our hospitals during the same period were selected as the colon polyps group. The differences in cingulin and claudin-2 protein expression in intestinal lesions, intestinal flora distribution in feces specimens as well as indexes related to oxidative stress and inflammation in serum were compared between the two groups.Results: cingulin and claudin-2 protein expression in colon lesions of UC group were higher than those of colon polyps group;Escherichia coli count in intestinal tract was higher than that of colon polyps group whereas bifidobacterium and lactobacillus count were lower than those of colon polyps group;oxidative stress indexes GSH-Px and T-AOC contents in serum were lower than those of colon polyps group whereas ROS and LHP contents were higher than those of colon polyps group;inflammatory factors IL-4, IL-6, IL-17, IL-23 and TGF-β contents in serum were higher than those of colon polyps group. Correlation analysis showed that the cingulin and claudin-2 protein expression in UC lesion tissues were directly correlated with the intestinal flora disturbance, oxidative stress response indexes and inflammatory response indexes. Conclusion: Abnormal high expression of cingulin and claudin-2 in UC lesions can promote the intestinal flora disturbance and systemic inflammatory stress response.展开更多
文摘Objective:To investigate the correlation of cingulin and claudin-2 expression in ulcerative colitis (UC) tissues with intestinal flora disturbance, oxidative stress and inflammation. Methods: 70 patients with UC who were diagnosed in our hospitals between July 2013 and February 2018 were selected as the UC group, and 90 patients with colonic polyps who underwent colonoscopy in our hospitals during the same period were selected as the colon polyps group. The differences in cingulin and claudin-2 protein expression in intestinal lesions, intestinal flora distribution in feces specimens as well as indexes related to oxidative stress and inflammation in serum were compared between the two groups.Results: cingulin and claudin-2 protein expression in colon lesions of UC group were higher than those of colon polyps group;Escherichia coli count in intestinal tract was higher than that of colon polyps group whereas bifidobacterium and lactobacillus count were lower than those of colon polyps group;oxidative stress indexes GSH-Px and T-AOC contents in serum were lower than those of colon polyps group whereas ROS and LHP contents were higher than those of colon polyps group;inflammatory factors IL-4, IL-6, IL-17, IL-23 and TGF-β contents in serum were higher than those of colon polyps group. Correlation analysis showed that the cingulin and claudin-2 protein expression in UC lesion tissues were directly correlated with the intestinal flora disturbance, oxidative stress response indexes and inflammatory response indexes. Conclusion: Abnormal high expression of cingulin and claudin-2 in UC lesions can promote the intestinal flora disturbance and systemic inflammatory stress response.
