Long non-coding RNA H19 regulates neurogenesis of induced neural stem cells in a mouse model of closed head injury

Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.

3轨式横向对称分布6-PSS并联机构及性能优化

针对多滑块共轨式6-PSS并联机构存在的姿态工作空间小、容易奇异等问题,课题组提出一种3轨式分布的6-PSS并联机构(P为移动副,S为球副)。该并联机构杆件长度和轨道间距呈不均匀布置,以提升姿态工作空间;采用闭环矢量法进行位置分析,通过极限边界搜索法求解工作空间;基于运动/力传递指标对机构性能进行评估,并采用性能图谱法基于全域指标GTI进行机构尺寸优化。结果表明优化后机构运动/力传递性能有显著提升,同时姿态工作空间也得到提升。该机构在工程上有一定的实用性。

The determination of 52 elements in marine geological samples by an inductively coupled plasma optical emission spectrometry and an inductively coupled plasma mass spectrometry with a high-pressure closed digestion method

An improved analytical method to determine the content of 52 major, minor and trace elements in marine geological samples, using a HF-HCl-HNO_3 acid system with a high-pressure closed digestion method（HPCD）, is studied by an inductively coupled plasma optical emission spectrometry（ICP-OES） and an inductively coupled plasma mass spectrometry（ICP-MS）. The operating parameters of the instruments are optimized, and the optimal analytical parameters are determined. The influences of optical spectrum and mass spectrum interferences, digestion methods and acid systems on the analytical results are investigated. The optimal spectral lines and isotopes are chosen, and internal standard element of rhodium is selected to compensate for matrix effects and analytical signals drifting. Compared with the methods of an electric heating plate digestion and a microwave digestion, a high-pressure closed digestion method is optimized with less acid, complete digestion,less damage for digestion process. The marine geological samples are dissolved completely by a HF-HCl-HNO_3 system, the relative error（RE） for the analytical results are all less than 6.0%. The method detection limits are 2–40μg/g by the ICP-OES, and 6–80 ng/g by ICP-MS. The methods are used to determine the marine sediment reference materials（GBW07309, GBW07311, GBW07313）, rock reference materials（GBW07103, GBW07104,GBW07105）, and cobalt-rich crust reference materials（GBW07337, GBW07338, GBW07339）, the obtained analytical results are in agreement with the certified values, and both of the relative standard deviation（RSD） and the relative error（RE） are less than 6.0%. The analytical method meets the requirements for determining 52 elements contents of bulk marine geological samples.

Two-dimensional Parameter Relationships for W UMa-type Systems Revisited

Reviewing the empirical and theoretical parameter relationships between various parameters is a good way to understand more about contact binary systems.In this investigation,two-dimensional(2D)relationships for P–MV(system),P–L1,2,M1,2–L1,2,and q–Lratiowere revisited.The sample used is related to 118 contact binary systems with an orbital period shorter than 0.6 days whose absolute parameters were estimated based on the Gaia Data Release 3 parallax.We reviewed previous studies on 2D relationships and updated six parameter relationships.Therefore,Markov chain Monte Carlo and Machine Learning methods were used,and the outcomes were compared.We selected 22 contact binary systems from eight previous studies for comparison,which had light curve solutions using spectroscopic data.The results show that the systems are in good agreement with the results of this study.

Design and analysis of an advanced thermal management system for the solar close observations and proximity experiments spacecraft

In this paper,the mission and the thermal environment of the Solar Close Observations and Proximity Experiments(SCOPE)spacecraft are analyzed,and an advanced thermal management system(ATMS)is designed for it.The relationship and functions of the integrated database,the intelligent thermal control system and the efficient liquid cooling system in the ATMS are elaborated upon.For the complex thermal field regulation system and extreme space thermal environment,a modular simulation and thermal field planning method are proposed,and the feasibility of the planning algorithm is verified by numerical simulation.A solar array liquid cooling system is developed,and the system simulation results indicate that the temperatures of the solar arrays meet the requirements as the spacecraft flies by perihelion and aphelion.The advanced thermal management study supports the development of the SCOPE program and provides a reference for the thermal management in other deep-space exploration programs.

基于熵权-TOPSIS多目标评价模型的幸福河湖评价

为科学合理评价幸福河湖,从安澜之河、富民之河、宜居之河、生态之河、文化之河5个方面建立评价指标体系,筛选出具有代表性和可靠性的指标进行定量评价,并检验所构建幸福河湖评价指标体系的可靠性。建立基于熵权-TOPSIS的多目标评价模型,利用层次分析法、德尔菲法、熵权法(EWM)等得到综合权重,计算评价指标贴近度,得出河湖幸福度得分。以湖北省3条重要河流为评价对象,采用该模型进行评价,得到各条河流的幸福度得分,对标“幸福”标准找出差距。研究成果为明确河流存在的主要问题以及其解决提供了依据。

高层结构-混联Ⅱ型惯容阻尼耗能体系减震性分析

针对高层结构中设置混联Ⅱ型惯容阻尼器组成耗能体系的随机响应分析复杂的问题,提出了结构位移及阻尼器阻尼力响应的封闭解,并基于所获得的封闭解探究了实模态振型数对分析精度的影响和在原结构层间位移超限楼层设置阻尼器能有效减震的布置策略。首先,根据混联Ⅱ型惯容阻尼器力学构造图及设置方式,建立阻尼器阻尼力与结构节点水平位移的微分型本构关系进而重构耗能体系的地震动方程。其次,利用实模态解耦法获得高层结构简明等效的动力学参数,运用功率谱二次式分解法推导出结构节点相对于地面的绝对位移、竖向构件层间位移及阻尼器阻尼力等响应的功率谱精确二次式解进而推导出耗能体系基于双过滤白噪声激励下系列响应的0~2阶谱矩简明封闭解。最后,通过算例,验证了所提封闭解的正确性;研究了实模态振型数对系列响应谱矩分析精度的影响和阻尼器设置位置对结构减震效果的影响。研究表明:对于多自由度结构响应分析时,建议采用原结构自由振动分析时质量参与系数累计达100%时所对应的振型数,可获得稳定的精度和提高大型复杂结构设置混联Ⅱ型惯容阻尼器的响应的分析效率;通过在原结构层间位移超限楼层或连续多层层间位移超限的楼层中间隔一层设置参数合适的混联Ⅱ型惯容阻尼器可有效降低结构的地震动响应,所提惯容阻尼器设置策略具有较好的经济性。所提解析解和惯容系统在高层结构的设置策略可为混联Ⅱ型惯容阻尼器在实际工程应用中提供有益的参考价值。

覆岩应力-漏风综合影响下浅埋煤层群下煤层开采巷道布置研究

针对浅埋近距离煤层群上部遗留煤柱影响下的下部工作面巷道合理布置位置确定问题,以活鸡兔井为例,采用离散元方法(DEM)模拟研究了遗留煤柱底板应力分布与上、下煤层开采采空区裂隙发育特征。结果表明:12号煤层开采过后,22号煤层水平距上覆煤柱20 m内为垂向应力与主应力差增高区,22号煤层水平应力在区段煤柱内侧较低;下煤层巷道布置采用外错式与重叠式时,巷道两侧围岩主应力差较大,且外错式与重叠式布置方式下22号煤层工作面开采时夹层与上部老空区覆岩二次运移剧烈,采空区两侧斜向裂缝带发育与贯通较好;采用内错式,巷道两侧围岩主应力差随与煤柱距离增大先降低、后升高,在距煤柱25~35 m处主应力差达到最低,且该布置方式下上部采空区覆岩沉降均衡,裂缝带闭合性较好,极大阻止了地表与层间贯通漏风通道的发育。

Effects of Different Density-reducing Methods on Canopy Microenvironment, Tree Growth and Fruit Quality in Closed Apple Orchard

In order to optimize and transform closed mature apple orchards with standard rootstocks and improve the quality of fruit,taking a closed Red Fuji apple orchard as the test object,the effects of different density-reducing methods(deinterlacing,removing every other plant in each row,removing every other plant every other row)on the canopy microenvironment,tree structure,leaf photosynthesis and fruit quality were studied.The results showed that different density-reducing methods significantly reduced the orchard coverage and increased the crown transmittance.Among them,the deinterlacing treatment was the best in improving the population structure of the closed orchard,as it reduced the orchard coverage rate by 55.68 percentage points and the canopy transmittance by 82.38 percentage points,compared with the control(CK).Different density-reducing methods all could significantly reduce the branch amount in the closed orchard and optimized the branch composition.The three density-reducing methods decreased the number of branches per plant by 18.96%,12.41%and 19.58%,respectively,compared with the CK.And compared with the CK,the proportion of short branches and leafy branches to the total branches was increased by 17.13%,14.27%and 7.37%,respectively,and the proportion of long branches and developmental branches to the total branches was decreased by 24.47%,18.04%and 10.79%,respectively.The effects of the different density-reducing methods on the temperature,relative light intensity,SPAD and leaf photosynthetic rate in canopies all followed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing>removing every other plant in each row>removing every other plant every other row>CK,while those on the relative humidity showed an order of deinterlacing<removing every other plant in each row<removing every other plant every other row<CK.The average single fruit weight(238.3 g),coloring index(89.2),smoothness index(83.2),soluble solid content(15.1%)and high quality fruit rate(82.4%)of the deinterlacing treatment were higher than those of other treatments,and the values were 18.2%,11.4%,5.85%,26.9%and 25.2%higher than the CK,respectively.The use of dein￣terlacing to reduce density is the best for improving the microenvironment of closed apple orchards and improving the photosynthetic efficiency and fruit quality.

Subsystem model-based close-loop grey-box identification method for hydraulic stewart platform

In order to solve the problem of difficult modeling and identification caused by time-variable parameters,multiple inputs and outputs and unstable open loop,a subsystem model-based close-loop grey-box identification method was put forward when consider the main coupling effects of hydraulic Stewart platform.Firstly,the whole system is divided into three TITO(Two Input Two Output) subsystems according to the characteristics of the pseudo-mass matrix,hence transfer function matrix model of the subsystem can also be found.Secondly,since the Stewart platform is unstable,the close-loop transfer model of the subsystem is derived under the proportional controllers.The inverse M serial is adopted as the identification signal to get the experimental data.All parameters of the subsystem are determined in close-loop indirect identification by PEM(Prediction Error Method).Finally,a case study validates the correctness and effectiveness of the subsystem model-based close-loop grey-box identification method for hydraulic Stewart platform.

Adequate Closed Form Wave Solutions to the Generalized KdV Equation in Mathematical Physics

In this paper, we consider the generalized Korteweg-de-Vries (KdV) equations which are remarkable models of the water waves mechanics, the shallow water waves, the quantum mechanics, the ion acoustic waves in plasma, the electro-hydro-dynamical model for local electric field, signal processing waves through optical fibers, etc. We determine the useful and further general exact traveling wave solutions of the above mentioned NLDEs by applying the exp(−τ(ξ))-expansion method by aid of traveling wave transformations. Furthermore, we explain the physical significance of the obtained solutions of its definite values of the involved parameters with graphic representations in order to know the physical phenomena. Finally, we show that the exp(−τ(ξ))-expansion method is convenient, powerful, straightforward and provide more general solutions and can be helping to examine vast amount of travelling wave solutions to the other different kinds of NLDEs.

基于ARCHARD磨损模型的WR-CVT非连续接触钢丝绳磨损研究

针对WR-CVT(Wire rope continuously variable transmission)弯曲段钢丝绳更易磨损问题,以6×7+IWS(Merallic wire strand core)钢丝绳为研究对象,基于Archard磨损模型结合自适应网格技术,建立WR-CVT钢丝绳非连续接触磨损有限元模型,研究磨损对钢丝绳接触压应力以及滑移幅值等接触参量的影响,并进行了试验揭示磨损机理.结果表明:随着磨损的作用,钢丝间接触区域不断增大.最大磨损深度出现在钢丝绳与绳槽边缘接触处,此处钢丝接触压应力较磨损前显著减小,滑移幅值和磨损深度在不断增大,接触压应力集中点沿钢丝轴向不断移动,接触区域形貌逐渐形成扁平状,磨损机理为磨粒磨损、黏着磨损和疲劳磨损.

基于AHP-EWM-TOPSIS模型的电梯品牌评价方法

为有效指导使用单位正确选择新装电梯的品牌,通过层次分析法(AHP法)的理念建立评价体系,含4个一级指标及20个二级指标;用AHP法和熵权法(EWM法)求得综合权重,最后采用贴近理想解排序法(TOPSIS法)分析贴近度,确定方案优劣性。结果表明:该方法能够避免主客观因素的影响及单一方法的决策失误,选择有效的评价指标,使得结果更贴近实际。可见该方法不仅对电梯品牌的选择,而且对电梯的安全监管也有一定的指导意义。

Method with reliable accuracy and fast speed for measuring operational current of HTS NI closed-loop coils in steady persistent-current-mode

This study proposes a method for measuring the operational current of high temperature superconducting(HTS)non‐insulation(NI)closed‐loop coils,which operate in the steady persistent‐current‐mode(PCM).HTS NI closed‐loop coils are promising for many easily‐quenching direct‐current(DC)applications,where their performance is determined by magnetomotive forces,total number of turns,and dimensions.As the primary interface parameter in an application system,the operational current must be accurately and rapidly measured.Generally,this is achieved by dividing the measured magnetic field by the coil constant.However,even if the influence of the screening current induced field(SCIF)is not considered,existing methods for the coil constant may be disturbed by the performance and location of Hall sensors,or experience a long measuring period.Therefore,a relatively accurate and fast method is proposed in this study,which is based on adjusting the output current of the adjustable power supply and monitoring the coil voltage as an indicator.The proposed method was validated through experiments and simulations using an equivalent circuit model coupled with a finite element method(FEM)model,and its current accuracy can be equivalent to the resolution of the employed power supply.It was demonstrated that this method reduced the requirements for Hall sensor’s performance and location,and has a more reliable accuracy in contrast to the simulation method.Compared to the experimentally conventional method,the proposed method presents a significantly faster speed.The impact of the SCIF was considered and proven to be negligible for the tested pancake coils.Even for coils whose coil constant vibrates owing to the SCIF,this method can be adapted to directly measure various operational currents.Furthermore,it was demonstrated that the measurement error can be influenced by the current discrepancy among turns when the coil is not in the steady PCM,and a procedure for reducing this error was proposed.

A hyperbolic Lindstedt-Poincare method for homoclinic motion of a kind of strongly nonlinear autonomous oscillators

A hyperbolic Lindstedt-Poincare method is presented to determine the homoclinic solutions of a kind of nonlinear oscillators, in which critical value of the homoclinic bifurcation parameter can be determined. The generalized Lienard oscillator is studied in detail, and the present method＇s predictions are compared with those of Runge-Kutta method to illustrate its accuracy.

Optical fiber positioning based on four-quadrant detector with Gaussian fitting method

With the development of large-scale spectral surveys, fiber positioning technology has been developing rapidly. Because of the performance advantages of a four-quadrant（4Q） detector, a fiber positioning and real-time monitoring system based on the 4Q detector is proposed. The detection accuracy of this system is directly determined by the precision of the center of the spot. A Gaussian fitting algorithm based on the 4Q detector is studied and applied in the fiber positioning process to improve the calculated accuracy of the spot center. The relationship between the center position of the incident spot and the detector output signal is deduced. An experimental platform is built to complete the simulated experiment. Then we use the Gaussian fitting method to process experimental data, compare the fitting value with the theoretical one and calculate the corresponding error.

Surface and Texture Properties of Tb-Doped Ceria-Zirconia Solid Solution Prepared by Sol-Gel Method

The three-way catalysts （TWCs） promoters Ce0.6Zr0.4- x TbxO2-y were prepared by sol-gel method. BET surface areas analysis indicated that an increase of the dopant Tb content from x = 0.05 to x = 0.15 favors an increase of surface area from 66.8 to 80.4 m^2· g^-1 compared with the undoped sample Ce0 .6oZr0.40O2 65.1 m^2·g^- 1 after calcination at 650℃. Transmission electron microscopy （TEM） observation indicated that the doped samples have a higher thermal stability. The XRD and Raman spectra confirmed that the Ce0.6Zr0.4-xTbxO2-y cubic solid solution is formed. XPS analysis revealed that Ce and Tb mainly existed in the form of Ce^4＋ and Tb^3 ＋ , and Zr existed in the form of Zr^4＋ on the surface of the samples. The doped samples were homogenous in composition ; the introduction of Tb into the CeO2-ZrO2 promoters resuited in the formation of a solid solution, and the concentration of surface lattice oxygen was increased.

Construction of n-sided polygonal spline element using area coordinates and B-net method

In general, triangular and quadrilateral elements are commonly applied in two-dimensional finite element methods. If they are used to compute polycrystalline materials, the cost of computation can be quite significant. Polygonal elements can do well in simulation of the materials behavior and provide greater flexibility for the meshing of complex geometries. Hence, the study on the polygonal element is a very useful and necessary part in the finite element method. In this paper, an n-sided polygonal element based on quadratic spline interpolant, denoted by PS2 element, is presented using the triangular area coordinates and the B-net method. The PS2 element is conforming and can exactly model the quadratic field. It is valid for both convex and non-convex polygonal element, and insensitive to mesh distortions. In addition, no mapping or coordinate transformation is required and thus no Jacobian matrix and its inverse are evaluated. Some appropriate examples are employed to evaluate the performance of the proposed element.

Multi-train modeling and simulation integrated with traction power supply solver using simplified Newton–Raphson method

Multi-train modeling and simulation plays a vital role in railway electrification during operation and planning phase. Study of peak power demand and energy consumed by each traction substation needs to be deter- mined to verify that electrical energy flowing in its railway power feeding system is appropriate or not. Gauss-Seidel, conventional Newton-Raphson, and current injection methods are well-known and widely accepted as a tool for electrical power network solver in DC railway power supply study. In this paper, a simplified Newton-Raphson method has been proposed. The proposed method employs a set of current-balance equations at each electrical node instead of the conventional power-balance equation used in the conventional Newton-Raphson method. This concept can remarkably reduce execution time and computing complexity for multi-train simulation. To evaluate its use, Sukhumvit line of Bangkok transit system （BTS） of Thai- land with 21.6-km line length and 22 passenger stopping stations is set as a test system. The multi-train simulation integrated with the proposed power network solver is developed to simulate 1-h operation service of selected 5-min headway. From the obtained results, the proposed method is more efficient with approximately 18 % faster than the conventional Newton-Raphson method and just over 6 % faster than the current injection method.

A hybrid vertex-centered finite volume/element method for viscous incompressible flows on non-staggered unstructured meshes

This paper proposes a hybrid vertex-centered fi- nite volume/finite element method for solution of the two di- mensional （2D） incompressible Navier-Stokes equations on unstructured grids. An incremental pressure fractional step method is adopted to handle the velocity-pressure coupling. The velocity and the pressure are collocated at the node of the vertex-centered control volume which is formed by join- ing the centroid of cells sharing the common vertex. For the temporal integration of the momentum equations, an im- plicit second-order scheme is utilized to enhance the com- putational stability and eliminate the time step limit due to the diffusion term. The momentum equations are discretized by the vertex-centered finite volume method （FVM） and the pressure Poisson equation is solved by the Galerkin finite el- ement method （FEM）. The momentum interpolation is used to damp out the spurious pressure wiggles. The test case with analytical solutions demonstrates second-order accuracy of the current hybrid scheme in time and space for both veloc- ity and pressure. The classic test cases, the lid-driven cavity flow, the skew cavity flow and the backward-facing step flow, show that numerical results are in good agreement with the published benchmark solutions.