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共培养条件对农杆菌转化大豆子叶节的影响 被引量:5
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作者 李桂兰 刘晨光 +3 位作者 乔潇 杨晓倩 王迪 乔亚科 《核农学报》 CAS CSCD 北大核心 2014年第9期1567-1575,共9页
为提高大豆的遗传转化效率,利用根癌农杆菌对大豆成熟种子的子叶节进行遗传转化,从影响农杆菌和大豆子叶节外植体双方的因素出发,重点研究共培养阶段培养基的pH值、光照条件和共培养时间三个因素对转化的影响,以寻找适宜农杆菌和外植体... 为提高大豆的遗传转化效率,利用根癌农杆菌对大豆成熟种子的子叶节进行遗传转化,从影响农杆菌和大豆子叶节外植体双方的因素出发,重点研究共培养阶段培养基的pH值、光照条件和共培养时间三个因素对转化的影响,以寻找适宜农杆菌和外植体的平衡点。分析单因素试验和正交试验的研究结果表明,在共培养阶段,培养基pH值适宜范围为6.0~6.6,pH值以6.6为佳;提供10~16h·d-1光照均提高了转化效率,与完全黑暗差异显著;延长共培养时间显著提高转化效率,以7~10d为宜。本研究范围内以冀豆12、豫豆22和秦黄小粒12三个基因型大豆获得了较高转化效率,供试的6个大豆品种均获得了转基因植株,PCR和Southern分析表明植酸酶基因phyA基因已经整合到大豆基因组中。该结果对提高大豆的遗传转化效率有重要参考价值。 展开更多
关键词 农杆菌 大豆子叶节 共培养基pH值 共培养时间 共培养光照
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Flares before and after coronal mass ejections
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作者 Gadikere Sheshagiriyappa Suryanarayana 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2018年第3期99-104,共6页
Flare characteristics such as the flare occurrence number density and the distribution of peak flux as well as duration of flares occurring on either side of a coronal mass ejection(CME) onset time are studied. Whil... Flare characteristics such as the flare occurrence number density and the distribution of peak flux as well as duration of flares occurring on either side of a coronal mass ejection(CME) onset time are studied. While the flares are rather evenly distributed statistically on either side of the CME onset time,the flare peak flux and duration tend to decrease depending upon their occurrence either before or after the CME onset. This is consistent with the earlier findings that flares emit higher energy before a CME whereas the energy is less in flares occurring after a CME. 展开更多
关键词 SUN flares - Sun flare durations - Sun peak flux of flares - Sun coronal mass ejections
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