Objective:To investigate the protective effect of cobra venom factor(CVF)on lung tissue injury in rats with sepsis induced by cecal puncture,with a view to providing theoretical basis for the development of new anti-s...Objective:To investigate the protective effect of cobra venom factor(CVF)on lung tissue injury in rats with sepsis induced by cecal puncture,with a view to providing theoretical basis for the development of new anti-sepsis drugs.Methods:A total of 144 rats were randomly divided into sham operation group,sepsis group and CVF group,with 48 rats in each group.Cecal ligation and puncture(CLP)was used to establish a rat model of sepsis.And 8 rats were taken from each group at 2,4,8,12,16and 24 h to observe the differences in lung wet/dry weight ratio,lung histopathological changes,and serum inflammatory factor levels.Results:CVF treatment significantly alleviated the degree of pulmonary edema and swelling in septic rats,slowed down the trend of elevated lung wet/dry weight ratio,and reduced the pathological damage of lungs.The degree of vascular congestion,edema and inflammatory cell infiltration in lung tissue of the sepsis group increased with time,and the degree of lesions in the CVF group was milder than that in the sepsis group.The TNF-αlevel in lung tissue of septic rats was significantly down-regulated by CVF treatment,and the TNF-αlevels in the CVF group during the 8-24 h period ranged from[8 h:(1.47±0.14)ng/ml]to[24 h:(1.14±0.03)ng/ml],which was closer to that in sham operation group.Meanwhile,the IL-10level decreased from(91.3±5.5)ng/L to(39.8±13.4)ng/L during 8-24 h,which was higher than other groups.Conclusion:CVF can play a protective role against injury by down-regulating TNF-αand up-regulating IL-10 pathways,which can serve to alleviate focal lung injury and pulmonary edema from sepsis.展开更多
目的观察中华眼镜蛇毒活性组分(Ch ina cobra venomactive factor,CCVAF)对内皮细胞增殖的选择性抑制作用。方法实验分对照组和不同浓度的CCVAF组,应用MTT法分析CCVAF对牛肺主动脉内皮细胞(bovine arteria pu lmona-lis vascu lar endot...目的观察中华眼镜蛇毒活性组分(Ch ina cobra venomactive factor,CCVAF)对内皮细胞增殖的选择性抑制作用。方法实验分对照组和不同浓度的CCVAF组,应用MTT法分析CCVAF对牛肺主动脉内皮细胞(bovine arteria pu lmona-lis vascu lar endothelial cells,BAVEC)、SD大鼠主动脉血管平滑肌细胞(smooth musc le cells,SMC)、人胚肺成纤维细胞HLF以及人支气管上皮细胞HBE生长增殖的影响;并运用直线回归分析统计对比CCVAF在不同时间点对BAVEC的IC50及上述各细胞的24 h IC50。结果CCVAF呈剂量及时间依赖性抑制内皮细胞的增殖:6、12、24、48、72 h其IC50分别为4.955、4.932、2.443、3.048、3.133 mg.L-1,24 h抑制作用最强;观察此时浓度与抑制作用的关系,0.625、1.25、2.5、5、10、20 mg.L-1,抑制率分别是4.41%、30.94%、61.81%、86.17%、96.05%、98.68%,10 mg.L-1抑制率即达高峰,且各浓度组抑制作用与对照组相比差异有显著性(P<0.05)。CCVAF抑制内皮细胞增殖具有一定的选择性,作用24 h后,抑制BAVEC生长的作用明显高于其它3种细胞,对BAVEC、HLF、HBE、SMC的24 h IC50分别为2.443、22.233、35.318、32.810 mg.L-1。结论CCVAF能够选择性抑制内皮细胞增殖,这在抗血管生成及其靶向治疗研究中可能具有重要意义。展开更多
文摘Objective:To investigate the protective effect of cobra venom factor(CVF)on lung tissue injury in rats with sepsis induced by cecal puncture,with a view to providing theoretical basis for the development of new anti-sepsis drugs.Methods:A total of 144 rats were randomly divided into sham operation group,sepsis group and CVF group,with 48 rats in each group.Cecal ligation and puncture(CLP)was used to establish a rat model of sepsis.And 8 rats were taken from each group at 2,4,8,12,16and 24 h to observe the differences in lung wet/dry weight ratio,lung histopathological changes,and serum inflammatory factor levels.Results:CVF treatment significantly alleviated the degree of pulmonary edema and swelling in septic rats,slowed down the trend of elevated lung wet/dry weight ratio,and reduced the pathological damage of lungs.The degree of vascular congestion,edema and inflammatory cell infiltration in lung tissue of the sepsis group increased with time,and the degree of lesions in the CVF group was milder than that in the sepsis group.The TNF-αlevel in lung tissue of septic rats was significantly down-regulated by CVF treatment,and the TNF-αlevels in the CVF group during the 8-24 h period ranged from[8 h:(1.47±0.14)ng/ml]to[24 h:(1.14±0.03)ng/ml],which was closer to that in sham operation group.Meanwhile,the IL-10level decreased from(91.3±5.5)ng/L to(39.8±13.4)ng/L during 8-24 h,which was higher than other groups.Conclusion:CVF can play a protective role against injury by down-regulating TNF-αand up-regulating IL-10 pathways,which can serve to alleviate focal lung injury and pulmonary edema from sepsis.
文摘目的观察中华眼镜蛇毒活性组分(Ch ina cobra venomactive factor,CCVAF)对内皮细胞增殖的选择性抑制作用。方法实验分对照组和不同浓度的CCVAF组,应用MTT法分析CCVAF对牛肺主动脉内皮细胞(bovine arteria pu lmona-lis vascu lar endothelial cells,BAVEC)、SD大鼠主动脉血管平滑肌细胞(smooth musc le cells,SMC)、人胚肺成纤维细胞HLF以及人支气管上皮细胞HBE生长增殖的影响;并运用直线回归分析统计对比CCVAF在不同时间点对BAVEC的IC50及上述各细胞的24 h IC50。结果CCVAF呈剂量及时间依赖性抑制内皮细胞的增殖:6、12、24、48、72 h其IC50分别为4.955、4.932、2.443、3.048、3.133 mg.L-1,24 h抑制作用最强;观察此时浓度与抑制作用的关系,0.625、1.25、2.5、5、10、20 mg.L-1,抑制率分别是4.41%、30.94%、61.81%、86.17%、96.05%、98.68%,10 mg.L-1抑制率即达高峰,且各浓度组抑制作用与对照组相比差异有显著性(P<0.05)。CCVAF抑制内皮细胞增殖具有一定的选择性,作用24 h后,抑制BAVEC生长的作用明显高于其它3种细胞,对BAVEC、HLF、HBE、SMC的24 h IC50分别为2.443、22.233、35.318、32.810 mg.L-1。结论CCVAF能够选择性抑制内皮细胞增殖,这在抗血管生成及其靶向治疗研究中可能具有重要意义。
