过量表达LAR3和Bbchit1以提高毛白杨对叶枯病的抗性

利用转基因技术将多种抗病基因共同转入毛白杨中以提高其抗性,从而获得毛白杨抗病新品种是目前解决杨树真菌病害的主要研究方向之一。本研究通过根癌农杆菌介导的二次遗传转化,将来源于球孢白僵菌几丁质酶基因Bbchit1转入过量表达无色花色素还原酶基因LAR3转基因毛白杨中,实时定量PCR显示Bbchit1与LAR3均能有效表达,离体抗病试验显示Bbchit1+LAR3共表达转基因毛白杨细胞粗提液对杨树叶枯病菌具有明显抑制作用,进一步将叶枯病菌接种在转基因和非转基因毛白杨叶片上培养30天,转基因植株的感病面积均低于非转基因植株且Bbchit1+LAR3共表达转基因株系抗病效果更明显。上述抗病试验结果表明:LAR3和Bbchit1在杨树中共表达可提高其对叶枯病的抗性。

毛白杨二次遗传转化体系的建立

为了建立有效的毛白杨二次遗传转化体系,优化了培养基激素配比、抗生素筛选浓度和乙酰丁香酮浓度。采用卡那霉素和潮霉素筛选,GUS组织染色和基因组DNA的PCR检测,通过二次遗传转化将GUS基因和潮霉素磷酸转移酶基因Hpt导入到毛白杨中,获得了60.7%二次转化频率。

受限直方图均衡化的低频DCT系数重变换算法

针对现实人脸识别中由于光照、表情、姿态或其他物体引起的面部遮挡而严重影响识别率的问题,提出了受限直方图均衡化的低频DCT系数重变换算法。首先,将图像划分成多个互不重叠的局部小块,使用受限直方图均衡化对局部子块进行局部对比拉伸以实现去噪;然后,通过缩减适当数目的低频DCT系数来消除人脸图像中的光照变化;最后,利用核主成分分析进行特征提取,最近邻分类器完成最终的人脸识别。在ORL、扩展Yale B及1个户外人脸数据库上的实验验证了所提算法的有效性及鲁棒性,表明相比几种线性表示算法,本文算法在处理鲁棒人脸识别时取得了更高的识别率。

CLAHE融合低频DCT系数重变换的人脸识别算法

针对现实人脸识别中由于伪装(如围巾、太阳镜和头发)或其它物体引起的面部遮挡而严重影响识别率的问题,提出了CLAHE融合低频DCT系数重变换的人脸识别算法。首先将图像划分成多个互不重叠的局部小块,使用受限直方图均衡化对局部子块进行局部对比拉伸以实现去噪;然后,通过缩减适当数目的低频DCT系数来消除人脸图像中的光照变化;最后,利用核主成分分析进行特征提取,最近邻分类器完成最终的人脸识别。在扩展Yale B、FRGC V2.0及一个户外人脸数据库上的实验验证了所提算法的有效性及鲁棒性。实验结果表明,相比几种线性表示算法,改进算法在处理鲁棒人脸识别时取得了更高的识别率。

资源型城市的再次转型研究——以焦作市为例

以焦作市为例,剖析了其在资源型城市转型成绩背后存在的产业比例失调、能源消耗偏高、生态赤字严重等问题。为实现可持续发展,须再次转型。统筹产业、城市空间结构、交通3要素,确立再次转型的总体思路,分别提出优化产业结构、重塑城市空间、梳理内外交通等策略,以期助力焦作市未来发展。

大别山杂岩区深层韧性剪切带研究

大别山杂岩区存在NWW向右行逆冲型和NNE向左行逆冲型深层韧性剪切带,其中发育一套具糜棱岩流变构造（宏观）和片麻岩结构（中粗粒）特征的岩石组合,本文称之为剪切片麻岩。研究认为,NWW向和NNE向深层韧性剪切带组成了近南北向强烈挤压背景下形成的韧性共轭剪切系统,其成因可能与早前寒武纪期间中国统一古陆与Laurentia超大陆相互作用相关。

以G418为筛选标记的籼稻二次遗传转化方法建立

为了研究籼稻有效的农杆菌介导二次遗传转化方法,以T-DNA插入突变体M1和M2幼胚为受体材料,通过G418浓度分别为100 mg/L和150 mg/L(20 d/代)的筛选培养基筛选获得的抗性胚性愈伤,转化率最高分别为10.91%和11.05%;分化生根获得M1和M2基因互补转基因水稻植株各143株和180株,经PCR检测阳性率分别达到72.0%和76.1%。试验结果表明,以G418为筛选标记的籼稻二次遗传转化方法,可以成为水稻遗传改良及基因功能研究的新方法。

消化液回输对重症急性胰腺炎患者疗效及营养状况分析

[目的]探究消化液回输对重症急性胰腺炎患者治疗效果及营养状况的改善。[方法]选取我院2016年8月至2018年8月收治的58例重症急性胰腺炎患者作为研究对象,将所有患者随机分成对照组和实验组两组,各29例。其中实验组患者进行消化液回输,对照组患者进行等量等渗盐水输入。比较治疗后两组患者的营养状况和治疗结局。[结果]治疗后,实验组患者的SOFA评分、APACHEⅡ评分均明显优于对照组,差异具有统计学意义(P<0.01);实验组患者的腹腔感染率小于对照组,差异有统计学意义(P<0.01);肠内营养达标率及BMI明显优于对照组,差异具有统计学意义(P<0.01);治疗后实验组患者的ALB、TP、Hb水平均明显优于对照组,差异具有统计学意义(P<0.01)。[结论]重症急性胰腺炎患者消化液回输,可有效改善患者的营养状况,促进肠道功能尽快恢复,值得临床推广应用。

弹性黏弹性对应原理在黏弹性固结有限层方法中的应用--数值篇

在黏弹性介质地基的比奥固结和基坑开挖问题有限层求解格式基础上,运用Laplace逆变换,编制了黏弹性介质地基位移计算的比奥固结和基坑开挖问题有限层求解程序,通过计算黏弹性地基沉降的算例,验证了计算方法与程序的正确性,随着黏滞性系数的增加,该模型的黏性起到更大的作用,符合所选取黏弹性模型的蠕变变化规律.

对称原理的进一步推广及研讨

利用分式线性照射,把关于两圆周对称的区域,分别照射成关于实轴对称的两区域,然后再建立后面这两个区域间的分式线性照射,最后利用代入法化简整理,便于得到关于两圆周对称的区域间的照射;同时还证得了在扩充的定义区域内上述函数的解析性态,以便于研究这些解析函数.

CLAHE优化低频DCT系数重变换的鲁棒人脸识别

针对光照、表情、姿态、遮挡等变化显著影响人脸识别系统性能的问题,提出了基于限制对比度自适应直方图均衡化(CLAHE)的低频离散余弦变换(DCT)系数重变换算法。将图像划分成多个互不重叠的局部小块,使用CLAHE对每个局部小块进行局部对比拉伸以实现去噪,通过缩减适当数目的低频DCT系数来消除人脸图像中的光照变化;利用核主成分分析进行特征提取,采用K-最近邻分类器以完成最终的人脸识别。在ORL、扩展YaleB和AR人脸数据库上的实验验证了所提算法的有效性和鲁棒性,实验结果表明,相比其他几种较为先进的人脸识别技术,所提算法取得了更高的识别率,同时大大降低了识别所用时间。

北约再转型悖论及中国与北约关系

当今世界正处于百年未有之大变局,中国日益走近世界舞台的中央,美国公开将中国视为战略敌手,北约开始寻求利用中国这一新的话题来增强其合法性。北约在伦敦首脑峰会中首次将中国议题纳入议程,凸显了其对中国崛起的关注,中国与北约关系的走向备受瞩目。北约自冷战结束后一直寻求通过转型应对生存危机,应对中国崛起能否成为其再转型过程中解决合法性危机的新选择。传统与非传统安全的双重回归,中国崛起的"威胁感知"的确使北约开始重新审视自己的战略并思考再转型之路,但是北约的再转型却面临三大悖论,涉及北约扩大的悖论(即不扩大,合法性不足;扩大,合法性受损),欧洲追求战略自主与美国保持战略优势的悖论,以及北约核心任务的全球扩张与聚焦成员安全的悖论。在面临再转型悖论的情况下,北约很可能将中国当成特定领域的竞争对手,而非整体上的敌人。中国要灵活主动地处理与北约的关系,在合作中趋利避害,共同解决人类面临的安全威胁,以人类命运共同体超越集体安全与军事联盟的逻辑。

CLAHE低频DCT系数重变换在人脸识别中的应用

针对光照变化显著影响自动人脸识别系统性能的问题,为了规范化光照变化以提高人脸识别率,提出基于对比度限制自适应直方图均衡化CLAHE(Contrast Limiting Adaptive Histogram Equalization)的低频离散余弦变换DCT(Discrete Cosine Transform)系数重变换算法。首先将图像划分成多个互不重叠的局部小块,使用CLAHE对局部小块进行局部对比拉伸以实现去噪;然后,通过缩减适当数目的低频DCT系数来消除人脸图像中的光照变化;最后,利用核主成分分析进行特征提取,稀疏系数重建和k-近邻分类器完成最终的人脸识别。在扩展Yale B及AR人脸数据库上的实验验证了算法的有效性。实验结果表明,算法在识别非常困难的Yale B子集5上的识别率可高达98.20%,相比其他几种规范化技术,该算法取得了更高的识别率,同时大大降低了识别所耗时间。

PREPATATION OF SPODUMENE-DERIVED LOW EXPANSION COEFFICIENT GLASS CERAMIC IN THE SYSTEM Li_2O-Al_2O_3-SiO_2

In this paper ,spodumene mineral was used as raw material for fabricating glass ceramic with the addition of MgO,ZnO and TiO2,ZrO2. The expansion coefficient of the materials is 5. 5×107/℃ . Also the effects of the heat treatment on the crystallzing behaviour has been discussed by XRD and DTA.

Self-excited vibration of driveline for vehicle launch

The launch shudder phenomenon induced by self-excited vibration of driveline was stud- ied with a compact car equipped with AMT as research object. The research showed that self-excited vibration was closely related with damping of driveline, the variation of friction coefficient, equiva- lent radius of friction plate and applied force of pressure plate. Six DOFs torsional vibration model of vehicle driveline was established according to the parameters of the certain compact car. The simula- tion was carried out and the result was compared with test data. It was found that the negative slope of friction coefficient with relative slip speed does not necessarily lead to self-excited vibration and the frequency of self-excited vibration on 1st gear is near to the 1st order of torsional natural frequen- cy. The influence of each viscous damping in driveline on self-excited vibration was analyzed by sim- ulation and the results showed that increasing the torsional dampings of half-axles and tires properly was effective to improve launch shudder phenomenon.

Phenol Oxidation by Combined Cavitation Water Jet and Hydrogen Peroxide

The paper presents results of phenol oxidized under the conditions of high temperature created during collapse of cavitation bubbles.The degradation efficiency has been greatly improved by using cavitation water jets combined with H2O2 as demonstrated in laboratory tests.Various factors affecting phenol removal ratio were ex-amined and the degradation mechanism was revealed by high performance liquid chromatography(HPLC).The re-sults showed that 99.85% of phenol was mineralized when phenol concentration was 100 mg·L-1 with pH value of 3.0,H2O2 concentration of 300 mg·L-1,confining pressure of 0.5 MPa,and pumping pressure of 20 MPa.The in-termediate products after phenol oxidation were composed of catechol,hydroquinone and p-benzoquinone.Finally,phenol was degraded into maleic acid and acetic acid.Furthermore,a dynamic model of phenol oxidation via cavi-tation water jets combined with H2O2 has been developed.

CLAHE融合低频DCT系数重变换的人脸识别算法

针对人脸识别中由于伪装(如围巾、太阳镜和头发)或其他物体引起的面部遮挡而严重影响识别率的问题,提出了CLAHE融合低频DCT系数重变换的人脸识别算法。首先将图像划分成多个互不重叠的局部小块,使用受限直方图均衡化对局部子块进行局部对比拉伸以实现去噪;然后,通过缩减适当数目的低频DCT系数来消除人脸图像中的光照变化;最后,利用核主成分分析进行特征提取,最近邻分类器完成最终的人脸识别。在扩展Yale B、FRGC V2.0及一个户外人脸数据库上的实验验证了所提算法的有效性及鲁棒性。实验结果表明,相比几种线性表示算法,本文算法在处理鲁棒人脸识别时取得了更高的识别率。

Slide stability of hydraulic structures on subbed soil

The study on slide stability of hydraulic structures on subbed soil was made. Using the slide test results of dragged concreting base plates on subbed soil pits, the decreased value of bearing capacity on slide after re- bound and repression influence of subbed soil was determined, and the envelope of ultimate slide shear resistance was also quantitatively determined. Due to the lack of similar mechanisms of slide stability on subbed soil and base plate of hydraulic structures, different safety coefficients for the slide stability were adopted. It was suggested to use the maximum compressive stress O＇m~ of eccentric load to predict structure displacement, slide and creepy slippage of subbed soil, to determine the sliding creepy contour and limit the maximum load on subbed soil. Two hydraulic structures that had been put into operation were reviewed by this method, and the results accorded with the real conditions.

腹水自虑回输治疗肝硬化顽固性腹水疗效观察

目的:探索腹水回输自滤的治疗方法在肝硬化伴顽固性腹水患者中的治疗效果,不良反应。方法:对54例肝硬化伴顽固性腹水患者,采取腹水静脉回输自滤的治疗方法,进行回顾性总结分析。向患者家属介绍该疗法的目的、方法及如何配合治疗,并正确评价治疗效果。治疗中要严格无菌操作,做好管道护理,注意观察尿量、生命体征和腹痛变化。加强对不良反应的观察及进行有效的干预,预防并发症。结果:54例患者中显效32例,有效17例,无效5例,总有效率90.7%,治疗期间未出现严重并发症。结论:腹水静脉回输自滤对肝硬化伴顽固性腹水患者疗效肯定。

Experimental research on thermal transport properties of crystallized palladium-based alloys

Palladium-based alloy is a kind of material with a high glass forming ability and can be easily formed into an amorphous state. After an annealing process, it can also be maintained at a crystallized state. To study the thermal and electrical transport properties of crystallized palladium-based alloys, the steady-state T-type method, standard four-probe method, and AC heating-DC detecting T-type method were used to measure the thermal conductivity, electrical conductivity, and Seebeck coeffi- cient of crystallized Pd4oNiloCu3oP2o and Pd43Nilo- Cu27P2o alloys respectively. The results show that compared to amorphous samples, the thermal conductivity and electrical conductivity of crystallized palladium-based alloys are significantly higher, while the Seebeck coeffi- cient is lower. The ratio of crystallized and amorphous thermal conductivity is higher for Pd43Ni10Cu27P2o alloy fiber which has a higher glass forming ability, while the ratio of electronic thermal conductivity almost remains constant for both alloy fibers. The results also show that the slope of electrical resistivity to temperature is a function of elemental composition for crystallized quaternary palla- dium-based alloy fibers. The sensitivity of thermal conductivity and electrical conductivity to the composition is high, while the correlation between Seebeck coefficient and composition is relatively weak.