Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive s...Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive stages.This study revealed a rice-specific 24-nt miRNA,miR1868,whose accumulation was suppressed by cold stress.Knockdown of MIR1868 increased seedling survival,pollen fertility,seed setting,and grain yield under cold stress,whereas its overexpression conferred the opposite phenotype.Knockdown of MIR1868 increased reactive oxygen species(ROS)scavenging and soluble sugar content under cold stress by increasing the expression of peroxidase genes and sugar metabolism genes,and its overexpression produced the opposite effect.Thus,MIR1868 negatively regulated rice cold tolerance via ROS scavenging and sugar accumulation.展开更多
Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ ...Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings.展开更多
The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress an...The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress and the recovery after chilling stress), and the survival rate and the freezing resistance of cuttings during cold acclimation at -3℃ were investigated. Results showed that cold acclimation not only increased the contents of total soluble protein and RNA, the survival rates and the freezing resistance of cuttings, decreased the activity of RNase, but also reduced the declining degree of total soluble protein and RNA contents, and the increasing level of RNase caused by chilling stress as compared with the controls. In addition, cold acclimation augmented the increase in the level of total soluble protein and RNA, and facilitated the decrease of RNase during the recovery periods. Further analysis found that the DNA content of all treatments kept relative stability at various periods. The changes in total soluble protein, RNA and RNase were closely related to the freezing resistance of cuttings. It appears that the increase of RNA content caused by cold acclimation induced decrease of RNase activity may be involved in the accumulation of total soluble protein and the induction of freezing resistance of cuttings.展开更多
Populus tomentosa seedlings for cold-acclimating were pretreated wit h or without 20% saccharose. Changes in the concentrations of total soluble sugar , the survival rates, and freezing tolerance of seedlings during ...Populus tomentosa seedlings for cold-acclimating were pretreated wit h or without 20% saccharose. Changes in the concentrations of total soluble sugar , the survival rates, and freezing tolerance of seedlings during cold acclimatio n were investigated. The results showed that cold acclimation increased the conc entrations of total soluble sugar, the survival rates and freezing tolerance. Co ld acclimation, combined with the saccharose-pretreatment, enhanced the above- ment ioned effect of cold acclimation, and obviously increased the concentrations of total soluble sugar, the survival rates and freezing tolerance of seedlings. Fur ther analysis found that the concentrations of total soluble sugar in branches i ncreased greater than that in leaves during both cold acclimation with or withou t the pretreatment of saccharose. Moreover, an increase of the concentrations of total soluble sugar in branches and leaves was closely related to the freezing tolerance of seedlings. The results indicate the accumulation of soluble sugar i n seedlings induced by cold acclimation may be involved in the induction of free zing tolerance .展开更多
该文以结晶紫中性红胆盐培养基(violet red bile agar,VRBA)组分为体系基础,通过筛选凝胶,正交试验优化凝胶、乳糖与胆盐添加量,以及添加乳糖发酵诱导剂,进行了大肠菌群测试片的研制。研究表明,使用卡拉胶代替琼脂作为载体,将卡拉胶、...该文以结晶紫中性红胆盐培养基(violet red bile agar,VRBA)组分为体系基础,通过筛选凝胶,正交试验优化凝胶、乳糖与胆盐添加量,以及添加乳糖发酵诱导剂,进行了大肠菌群测试片的研制。研究表明,使用卡拉胶代替琼脂作为载体,将卡拉胶、乳糖与胆盐的添加量分别优化为15、10、1.2 g/L,同时添加250 mg/L乳酰基-N脂酰基鞘氨醇作为乳糖发酵诱导剂,大肠菌群测试片的理化与微生物性能达到最优。测试片性能符合国标方法对VRBA培养基的控制要求;与国标大肠菌群平板计数方法相比,测试片方法在实际样本的检测结果无明显差异的同时,将检测周期缩短了24 h,结果观察更为直观,提升了对弱产气型目标菌的检测能力。该文所研制的大肠菌群测试片便捷快速准确,可适用于食品检测,并可为商业化测试片的研制提供借鉴意义。展开更多
Recent studies have shown that the R2R3-MYB transcription factor MdMYB73 is involved in salt stress response in apple. However, no research was done whether MdMYB73 mediated cold tolerance in apple or not. In this stu...Recent studies have shown that the R2R3-MYB transcription factor MdMYB73 is involved in salt stress response in apple. However, no research was done whether MdMYB73 mediated cold tolerance in apple or not. In this study, we found that the expression of MdMYB73 was obviously induced by cold stress. Functional analysis showed that MdMYB73 significantly increased cold tolerance in transgenic apple calli and Arabidopsis. Quantitative real-time PCR （qRT-PCR） assay indicated that the expression levels of cold-responsive genes including MdCBF2, MdCBF3, MdCBF4, and MdCBF5 were obviously enhanced in MdMYB73 transgenic calli, suggesting that MdMYB73 increased cold tolerance via C-repeat binding factor （CBF） cold response pathway. Finally, we found that soluble sugar, which provides an osmoticum for cells, was increased in MdMYB73 transgenic calli compared to that in the wild type control. These findings provide a new insights into how MdMYB73 is involved in cold stress response.展开更多
The relationship between vernalization requirement and quantitative and qualitative changes in total leaf soluble proteins were determined in one spring （cv. Kohdasht） and two winter （cvs. Sardari and Norstar） cul...The relationship between vernalization requirement and quantitative and qualitative changes in total leaf soluble proteins were determined in one spring （cv. Kohdasht） and two winter （cvs. Sardari and Norstar） cultivars of wheat （Triticum aestivum L.） exposed to 4℃. Plants were sampled on days 2, 14, 21 and 35 of exposure to 4℃. The final leaf number （FLN） was determined throughout the vernalization periods （0, 7, 14, 24, and 35 d） at 4℃. The final leaf number decreased until days 24 and 35 in Sardari and Norstar eultivars, respectively, indicating the vernalization saturation at these times. No clear changes were detected in the final leaf number of Kohdash cultivar, verifying no vernalization requirement for this spring wheat cultivar. Comparing with control, clear cold-induced 2-fold increases in proteins quantity occurred after 48 h following the 4℃-treatment in the leaves of the both winter wheat cultivars but, such response was not detected in the spring cultivar. However, the electrophoretic protein patterns showed between-cultivar and between-temperature treatment differences. With increasing exposure time to 4℃, the winter cultivars tended to produce more HMW polypeptides than the spring cultivar. Similar proteins were induced in both Sardari and Norstar winter wheat cultivars, however, the long vernalization requirement in Norstar resulted in high level and longer duration of expression of cold-induced proteins compared to Sardari with a short vernalization requirement. These observations indicate that vernalization response regulates the expression of low temperature （LT） tolerance proteins and determines the duration of expression of LT- induced proteins.展开更多
Rooftop gardening or green roof establishment, one of the methods of afforestation for urban greening, has many benefits for the environment, the economy and urban landscapes. Various environmental stresses including ...Rooftop gardening or green roof establishment, one of the methods of afforestation for urban greening, has many benefits for the environment, the economy and urban landscapes. Various environmental stresses including heat, strong wind, sunshine and cold prevent most plants from growing well in extensive green roof systems. For the establishment of urban rooftop gardening in Beijing, we evaluated 13 ornamental plant species [Berberis thunbergii ‘Atropurea Nana’, Euonymus fortune （Turcz.） Hand., Viburnum dilatatum Thunb., Cotinus coggygria Scop., Swida alba Opiz., Weigela florida （Bunge.） A. DC., Jasminum nudiflorum Lindl., Euonymus japonicus Thunb., Euonymus japonicus ‘Cuzhi’, Buddleja davidii Franch., Pyracantha fortuneana （Maxim.） Li., Ligustrum lucidum Ait. and Spiraea salicifolia L.] for greening of flat rooftops. We obtained three indices concerning cold resistance, i.e., electrical conductivity of leaves, amounts of soluble sugars and amounts of free proline, providing a theoretical basis for selection of plants fit for roof and balcony greening. The results show that the six species P. fortuneana （Maxim.） Li., B. thunbergii ‘Atropurea Nana’, J. nudiflorum Lindl., E. fortune （Turcz.） Hand., E. japonicus Thunb. and E. japonicus ‘Cuzhi’ are excellent plants for rooftop gardening, judging from their strong ability in cold resistance. Plants such as L. lucidum Ait., C. coggygria Scop. and W. florida （Bunge） A. DC. showed a medium ability in cold resistance. However, these species can be used in roof and balcony greening after intensive management or acclimatization of variety. The ability to resist cold of plants such as W. florida （Bunge） A. DC., S. salicifolia L., V. rhytidophyllum and S. alba Opiz. （Cornus alba L.） was found to be rather weak. Therefore, cold-proof measures are recommended when using these species.展开更多
In order to reveal the cold resistant mechanism of tea plant,two varieties with different cold resistances were studied.The activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD) and polyphenol oxidase(...In order to reveal the cold resistant mechanism of tea plant,two varieties with different cold resistances were studied.The activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD) and polyphenol oxidase(PPO) were investigated,and the contents of soluble protein,sugar and malondialdehyde(MDA) were determined in fresh tea leaves in the autumn.The results showed that the activities of POD and the content of soluble sugar were both much higher in the variety with strong cold resistance,and the content of soluble protein was associated with the cold resistance,while the cold resistance of the plant was not related with the activities of the CAT,SOD and PPO,and the content of MDA.It is considered that the activity of POD and contents of soluble sugar could be used as indicators to identify the cold resistance of tea varieties.展开更多
The solubility of C.I. reactive blue 19 in aqueous alkali is poor, so it isn't used to dye cellulosic fiber in cold pad-batch dyeing. In order to improve the solubility of this dyestuff in alkali liquor, the right...The solubility of C.I. reactive blue 19 in aqueous alkali is poor, so it isn't used to dye cellulosic fiber in cold pad-batch dyeing. In order to improve the solubility of this dyestuff in alkali liquor, the right dispersants will be needed. A series of condensates are synthesized by changing the synthesis conditions such as the ratio of naphthalenesulfonic (N) to formaldehyde (F), acidity, and their compositions are confirmed by MS spectrum. It is found that in acidity scope of 20%-24% and the ratio of N to F 1∶0.33, the synthesized condensates can efficiently improve the solubility of C.I. reactive blue 19 in alkali liquor. In addition, the influences of the condensates on the exhaust dyeing and the cold pad-batch dyeing are tested.展开更多
基金supported by grants from the National Natural Science Foundation of China(U20A2025,32101672,31971826)the National Key Research and Development Plan of China(2021YFF1001100)+2 种基金Natural Science Foundation of Heilongjiang province(YQ2023C035)Double First-class Innovation Achievement Program of Heilongjiang Province(LJGXCG2023-072)the Graduate Student Scientific Research Innovation Projects of Heilongjiang Bayi Agricultural University(YJSCX2022-Z01)。
文摘Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive stages.This study revealed a rice-specific 24-nt miRNA,miR1868,whose accumulation was suppressed by cold stress.Knockdown of MIR1868 increased seedling survival,pollen fertility,seed setting,and grain yield under cold stress,whereas its overexpression conferred the opposite phenotype.Knockdown of MIR1868 increased reactive oxygen species(ROS)scavenging and soluble sugar content under cold stress by increasing the expression of peroxidase genes and sugar metabolism genes,and its overexpression produced the opposite effect.Thus,MIR1868 negatively regulated rice cold tolerance via ROS scavenging and sugar accumulation.
文摘Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings.
文摘The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress and the recovery after chilling stress), and the survival rate and the freezing resistance of cuttings during cold acclimation at -3℃ were investigated. Results showed that cold acclimation not only increased the contents of total soluble protein and RNA, the survival rates and the freezing resistance of cuttings, decreased the activity of RNase, but also reduced the declining degree of total soluble protein and RNA contents, and the increasing level of RNase caused by chilling stress as compared with the controls. In addition, cold acclimation augmented the increase in the level of total soluble protein and RNA, and facilitated the decrease of RNase during the recovery periods. Further analysis found that the DNA content of all treatments kept relative stability at various periods. The changes in total soluble protein, RNA and RNase were closely related to the freezing resistance of cuttings. It appears that the increase of RNA content caused by cold acclimation induced decrease of RNase activity may be involved in the accumulation of total soluble protein and the induction of freezing resistance of cuttings.
文摘Populus tomentosa seedlings for cold-acclimating were pretreated wit h or without 20% saccharose. Changes in the concentrations of total soluble sugar , the survival rates, and freezing tolerance of seedlings during cold acclimatio n were investigated. The results showed that cold acclimation increased the conc entrations of total soluble sugar, the survival rates and freezing tolerance. Co ld acclimation, combined with the saccharose-pretreatment, enhanced the above- ment ioned effect of cold acclimation, and obviously increased the concentrations of total soluble sugar, the survival rates and freezing tolerance of seedlings. Fur ther analysis found that the concentrations of total soluble sugar in branches i ncreased greater than that in leaves during both cold acclimation with or withou t the pretreatment of saccharose. Moreover, an increase of the concentrations of total soluble sugar in branches and leaves was closely related to the freezing tolerance of seedlings. The results indicate the accumulation of soluble sugar i n seedlings induced by cold acclimation may be involved in the induction of free zing tolerance .
文摘该文以结晶紫中性红胆盐培养基(violet red bile agar,VRBA)组分为体系基础,通过筛选凝胶,正交试验优化凝胶、乳糖与胆盐添加量,以及添加乳糖发酵诱导剂,进行了大肠菌群测试片的研制。研究表明,使用卡拉胶代替琼脂作为载体,将卡拉胶、乳糖与胆盐的添加量分别优化为15、10、1.2 g/L,同时添加250 mg/L乳酰基-N脂酰基鞘氨醇作为乳糖发酵诱导剂,大肠菌群测试片的理化与微生物性能达到最优。测试片性能符合国标方法对VRBA培养基的控制要求;与国标大肠菌群平板计数方法相比,测试片方法在实际样本的检测结果无明显差异的同时,将检测周期缩短了24 h,结果观察更为直观,提升了对弱产气型目标菌的检测能力。该文所研制的大肠菌群测试片便捷快速准确,可适用于食品检测,并可为商业化测试片的研制提供借鉴意义。
基金supported by the National Natural Science Foundation of China (31601728,31272142,31325024)the Natural Science Foundation of Shandong Province,China (ZR2016CQ13)the Youth Science and Technology Innovation Fund of Shandong Agricultural University,China (24024)
文摘Recent studies have shown that the R2R3-MYB transcription factor MdMYB73 is involved in salt stress response in apple. However, no research was done whether MdMYB73 mediated cold tolerance in apple or not. In this study, we found that the expression of MdMYB73 was obviously induced by cold stress. Functional analysis showed that MdMYB73 significantly increased cold tolerance in transgenic apple calli and Arabidopsis. Quantitative real-time PCR （qRT-PCR） assay indicated that the expression levels of cold-responsive genes including MdCBF2, MdCBF3, MdCBF4, and MdCBF5 were obviously enhanced in MdMYB73 transgenic calli, suggesting that MdMYB73 increased cold tolerance via C-repeat binding factor （CBF） cold response pathway. Finally, we found that soluble sugar, which provides an osmoticum for cells, was increased in MdMYB73 transgenic calli compared to that in the wild type control. These findings provide a new insights into how MdMYB73 is involved in cold stress response.
基金financially supported by a grant from Tarbiat Modares University,Tehran,Iran
文摘The relationship between vernalization requirement and quantitative and qualitative changes in total leaf soluble proteins were determined in one spring （cv. Kohdasht） and two winter （cvs. Sardari and Norstar） cultivars of wheat （Triticum aestivum L.） exposed to 4℃. Plants were sampled on days 2, 14, 21 and 35 of exposure to 4℃. The final leaf number （FLN） was determined throughout the vernalization periods （0, 7, 14, 24, and 35 d） at 4℃. The final leaf number decreased until days 24 and 35 in Sardari and Norstar eultivars, respectively, indicating the vernalization saturation at these times. No clear changes were detected in the final leaf number of Kohdash cultivar, verifying no vernalization requirement for this spring wheat cultivar. Comparing with control, clear cold-induced 2-fold increases in proteins quantity occurred after 48 h following the 4℃-treatment in the leaves of the both winter wheat cultivars but, such response was not detected in the spring cultivar. However, the electrophoretic protein patterns showed between-cultivar and between-temperature treatment differences. With increasing exposure time to 4℃, the winter cultivars tended to produce more HMW polypeptides than the spring cultivar. Similar proteins were induced in both Sardari and Norstar winter wheat cultivars, however, the long vernalization requirement in Norstar resulted in high level and longer duration of expression of cold-induced proteins compared to Sardari with a short vernalization requirement. These observations indicate that vernalization response regulates the expression of low temperature （LT） tolerance proteins and determines the duration of expression of LT- induced proteins.
文摘Rooftop gardening or green roof establishment, one of the methods of afforestation for urban greening, has many benefits for the environment, the economy and urban landscapes. Various environmental stresses including heat, strong wind, sunshine and cold prevent most plants from growing well in extensive green roof systems. For the establishment of urban rooftop gardening in Beijing, we evaluated 13 ornamental plant species [Berberis thunbergii ‘Atropurea Nana’, Euonymus fortune （Turcz.） Hand., Viburnum dilatatum Thunb., Cotinus coggygria Scop., Swida alba Opiz., Weigela florida （Bunge.） A. DC., Jasminum nudiflorum Lindl., Euonymus japonicus Thunb., Euonymus japonicus ‘Cuzhi’, Buddleja davidii Franch., Pyracantha fortuneana （Maxim.） Li., Ligustrum lucidum Ait. and Spiraea salicifolia L.] for greening of flat rooftops. We obtained three indices concerning cold resistance, i.e., electrical conductivity of leaves, amounts of soluble sugars and amounts of free proline, providing a theoretical basis for selection of plants fit for roof and balcony greening. The results show that the six species P. fortuneana （Maxim.） Li., B. thunbergii ‘Atropurea Nana’, J. nudiflorum Lindl., E. fortune （Turcz.） Hand., E. japonicus Thunb. and E. japonicus ‘Cuzhi’ are excellent plants for rooftop gardening, judging from their strong ability in cold resistance. Plants such as L. lucidum Ait., C. coggygria Scop. and W. florida （Bunge） A. DC. showed a medium ability in cold resistance. However, these species can be used in roof and balcony greening after intensive management or acclimatization of variety. The ability to resist cold of plants such as W. florida （Bunge） A. DC., S. salicifolia L., V. rhytidophyllum and S. alba Opiz. （Cornus alba L.） was found to be rather weak. Therefore, cold-proof measures are recommended when using these species.
文摘In order to reveal the cold resistant mechanism of tea plant,two varieties with different cold resistances were studied.The activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD) and polyphenol oxidase(PPO) were investigated,and the contents of soluble protein,sugar and malondialdehyde(MDA) were determined in fresh tea leaves in the autumn.The results showed that the activities of POD and the content of soluble sugar were both much higher in the variety with strong cold resistance,and the content of soluble protein was associated with the cold resistance,while the cold resistance of the plant was not related with the activities of the CAT,SOD and PPO,and the content of MDA.It is considered that the activity of POD and contents of soluble sugar could be used as indicators to identify the cold resistance of tea varieties.
文摘The solubility of C.I. reactive blue 19 in aqueous alkali is poor, so it isn't used to dye cellulosic fiber in cold pad-batch dyeing. In order to improve the solubility of this dyestuff in alkali liquor, the right dispersants will be needed. A series of condensates are synthesized by changing the synthesis conditions such as the ratio of naphthalenesulfonic (N) to formaldehyde (F), acidity, and their compositions are confirmed by MS spectrum. It is found that in acidity scope of 20%-24% and the ratio of N to F 1∶0.33, the synthesized condensates can efficiently improve the solubility of C.I. reactive blue 19 in alkali liquor. In addition, the influences of the condensates on the exhaust dyeing and the cold pad-batch dyeing are tested.
